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The map label for this gene is fusA [H]

Identifier: 183221350

GI number: 183221350

Start: 2043288

End: 2045219

Strand: Reverse

Name: fusA [H]

Synonym: LEPBI_I1967

Alternate gene names: 183221350

Gene position: 2045219-2043288 (Counterclockwise)

Preceding gene: 183221351

Following gene: 183221349

Centisome position: 56.82

GC content: 44.57

Gene sequence:

>1932_bases
ATGAAATATTACCGAACAGTAGGAATTTTTGCACACATTGATTCGGGAAAAACTACCCTCACCGAACGGATATTATATGA
GTCTGGAAAAATTTCGGCGGTGGGTTCCATTGAAGATGGAAATACGGAATCGGATTCTTTACAAGAGGAGATTGAACGTG
GGATTTCCATTCGAACCACTTTCCATTCCTTACCTTGGCAAACCGACTTCGGGACATTCCAAATCCAATTAGTAGACACG
CCCGGTCATATTGACTTTCGAAACCAAGTCACGGATCTTCTCCCTGCGATGGAAACAGCGGTTGTGGTTTTGGAAGCAGG
TACAGTGGTACAATCGCAGGCACGACTCGTGATAGAAGAACTGCGAAAGGCAAGTGTGCCCATTGTCTTTTTTATTAACA
AACTGGACCGATTTGGTGATGAATACTTGGACACGCTGGTGTCCCTCGAGGAAATTTTAGGTGTGGCACCTGTTTGCCTA
TTTCAAAAAACAGAGTCAGGAGCATTAGAATACTATCTAAACCATTCTCATTCTTTTGCGAAGAAGGTGGATGAGGAACT
TCTCTCTTGGAATGATGAACTTTTGATTTCTTCTTGGAGCCAATCTTCGTCTTCGGCAGATTTTGCGAGGATCGGTTTGA
AGGATGGTCCCAAATCCGGAAAACTTTTTCCTGTGTATGGAGGTTCTGCTAAAACAGGGGAAGGGGTTCGGGAACTTTTA
GACCTCCTCCTTTGGACAAATCCCAAAGTTCCAACTGACATAATCCCATTGGCCTATCCTCTACTTGTTTTATCCCGAAG
GATGGCGGAGGGAATTGGTCCTTATGCCGTTGTGTATCCACTAAAGGATTTGAGCGAATTAGACCTTCTTTCCATGACGG
AAACCTTTCTCCAATCCGTTCCTTTGCCTCCGAACGCTTCGAACGCTGGTCTTTTCCAATTCATCGACCCGGAGACGGAA
GAAACAACCCAAGAGATCCATAAGGGAAAACTTGTCCTCTTGAAACACAATGAACGTTTTCTTCTGTATCCAGGTGAGCC
CGTTGCCTTACGGAATGTTCAGGGAATAGGAAACGAGAACCAAAAAGACCAACGTTCCCCAAGTCCCTTTTCTCTTGTGA
TTGAACCGGAAGAATCCTCGGACAAAGAGTTTTGGTTATGTCGCCTAGAGGAACTCGTGTGGGAAGACCCGGGGTATCAG
ACGGCAATCAAGGAAGATACAGGACAAATTGTCCTCTTTGGGCGAGGGGAACTCCATTTGGAAATTGGGATCAAACGAAT
TTCCGGGAAAACAGAAAAAAAACTCACTTTTAGTTCGATAAACATTGCCAAATTAGAGCTTTTTAAAAAAATGTCTCATA
AGGTTGCCCTAGAGCATCGTGCCTTTGAAGACCAAAAGTCAAGCGGCACGCTCATCGCAGTCCTGGAAGATACTGCCGAT
TTTTCGAAGCAAATTGCCTTCGAGGTAAGTCTTCCGGAAGAAGTAAAAAATTCGATAGAAATGTCCTTTAAGGAAGCCTG
CTTACACGGGTTTTACGGTGAGGAAGTTTGTGGTCTTAGGCTTCGTATTCTCTCTTATGAGATGCCGAAGGGAGACTTAC
AAACCACTCTCACTCTTCTCAAAGTAGCAATACTTGCGGGAGTGAAGGAATGTTTTCCGTCAAACACATATTTGGTTGGT
CCCCTCACTGAAATTGAAGTGATGGTAGACTCAGACCACTTAGGTGTAGTTCTTTCTGATCTAAGTCGCAGGGACGCAAA
GGTGGTTTCCATCATTGAGGCTGTGGCAGGGAAGAGTCACTTAAAAGCCAATGCACCGGCCCAAAACCTGCTTGGCTTTT
CAGGGGCTCTTAGAAACATGACCAAAGGGATTGGCATTTCTTGGGAAAGGACTGCTTTTACCTCTGAATTTTATGCAGTT
CTAAAGGAGTAA

Upstream 100 bases:

>100_bases
AACAAGGCATTCTCTCACTACCGCTGGTAGTAGTATCCAATCGATTCCAAACATTGATGAGCCAGGTGGAAACCTGGCTT
TTTTGTTTTAACACACCAAT

Downstream 100 bases:

>100_bases
AGAACCGAATATCGGGTCTTGCACCACGATTGAGGAGTAGATTCTAACAATGGCTAAAGAAAAATTTGACCGTTCAAAAC
CACACTTAAACATCGGAACA

Product: elongation factor G

Products: GDP; phosphate

Alternate protein names: EF-G [H]

Number of amino acids: Translated: 643; Mature: 643

Protein sequence:

>643_residues
MKYYRTVGIFAHIDSGKTTLTERILYESGKISAVGSIEDGNTESDSLQEEIERGISIRTTFHSLPWQTDFGTFQIQLVDT
PGHIDFRNQVTDLLPAMETAVVVLEAGTVVQSQARLVIEELRKASVPIVFFINKLDRFGDEYLDTLVSLEEILGVAPVCL
FQKTESGALEYYLNHSHSFAKKVDEELLSWNDELLISSWSQSSSSADFARIGLKDGPKSGKLFPVYGGSAKTGEGVRELL
DLLLWTNPKVPTDIIPLAYPLLVLSRRMAEGIGPYAVVYPLKDLSELDLLSMTETFLQSVPLPPNASNAGLFQFIDPETE
ETTQEIHKGKLVLLKHNERFLLYPGEPVALRNVQGIGNENQKDQRSPSPFSLVIEPEESSDKEFWLCRLEELVWEDPGYQ
TAIKEDTGQIVLFGRGELHLEIGIKRISGKTEKKLTFSSINIAKLELFKKMSHKVALEHRAFEDQKSSGTLIAVLEDTAD
FSKQIAFEVSLPEEVKNSIEMSFKEACLHGFYGEEVCGLRLRILSYEMPKGDLQTTLTLLKVAILAGVKECFPSNTYLVG
PLTEIEVMVDSDHLGVVLSDLSRRDAKVVSIIEAVAGKSHLKANAPAQNLLGFSGALRNMTKGIGISWERTAFTSEFYAV
LKE

Sequences:

>Translated_643_residues
MKYYRTVGIFAHIDSGKTTLTERILYESGKISAVGSIEDGNTESDSLQEEIERGISIRTTFHSLPWQTDFGTFQIQLVDT
PGHIDFRNQVTDLLPAMETAVVVLEAGTVVQSQARLVIEELRKASVPIVFFINKLDRFGDEYLDTLVSLEEILGVAPVCL
FQKTESGALEYYLNHSHSFAKKVDEELLSWNDELLISSWSQSSSSADFARIGLKDGPKSGKLFPVYGGSAKTGEGVRELL
DLLLWTNPKVPTDIIPLAYPLLVLSRRMAEGIGPYAVVYPLKDLSELDLLSMTETFLQSVPLPPNASNAGLFQFIDPETE
ETTQEIHKGKLVLLKHNERFLLYPGEPVALRNVQGIGNENQKDQRSPSPFSLVIEPEESSDKEFWLCRLEELVWEDPGYQ
TAIKEDTGQIVLFGRGELHLEIGIKRISGKTEKKLTFSSINIAKLELFKKMSHKVALEHRAFEDQKSSGTLIAVLEDTAD
FSKQIAFEVSLPEEVKNSIEMSFKEACLHGFYGEEVCGLRLRILSYEMPKGDLQTTLTLLKVAILAGVKECFPSNTYLVG
PLTEIEVMVDSDHLGVVLSDLSRRDAKVVSIIEAVAGKSHLKANAPAQNLLGFSGALRNMTKGIGISWERTAFTSEFYAV
LKE
>Mature_643_residues
MKYYRTVGIFAHIDSGKTTLTERILYESGKISAVGSIEDGNTESDSLQEEIERGISIRTTFHSLPWQTDFGTFQIQLVDT
PGHIDFRNQVTDLLPAMETAVVVLEAGTVVQSQARLVIEELRKASVPIVFFINKLDRFGDEYLDTLVSLEEILGVAPVCL
FQKTESGALEYYLNHSHSFAKKVDEELLSWNDELLISSWSQSSSSADFARIGLKDGPKSGKLFPVYGGSAKTGEGVRELL
DLLLWTNPKVPTDIIPLAYPLLVLSRRMAEGIGPYAVVYPLKDLSELDLLSMTETFLQSVPLPPNASNAGLFQFIDPETE
ETTQEIHKGKLVLLKHNERFLLYPGEPVALRNVQGIGNENQKDQRSPSPFSLVIEPEESSDKEFWLCRLEELVWEDPGYQ
TAIKEDTGQIVLFGRGELHLEIGIKRISGKTEKKLTFSSINIAKLELFKKMSHKVALEHRAFEDQKSSGTLIAVLEDTAD
FSKQIAFEVSLPEEVKNSIEMSFKEACLHGFYGEEVCGLRLRILSYEMPKGDLQTTLTLLKVAILAGVKECFPSNTYLVG
PLTEIEVMVDSDHLGVVLSDLSRRDAKVVSIIEAVAGKSHLKANAPAQNLLGFSGALRNMTKGIGISWERTAFTSEFYAV
LKE

Specific function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=138, Percent_Identity=42.7536231884058, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI25306283, Length=209, Percent_Identity=33.9712918660287, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI19923640, Length=209, Percent_Identity=33.9712918660287, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI25306287, Length=280, Percent_Identity=30.7142857142857, Blast_Score=105, Evalue=2e-22,
Organism=Homo sapiens, GI4503483, Length=146, Percent_Identity=32.1917808219178, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI157426893, Length=132, Percent_Identity=32.5757575757576, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI94966754, Length=136, Percent_Identity=30.8823529411765, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1789738, Length=192, Percent_Identity=35.4166666666667, Blast_Score=119, Evalue=7e-28,
Organism=Escherichia coli, GI1790835, Length=163, Percent_Identity=33.1288343558282, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1788922, Length=159, Percent_Identity=33.9622641509434, Blast_Score=86, Evalue=9e-18,
Organism=Escherichia coli, GI48994988, Length=153, Percent_Identity=30.0653594771242, Blast_Score=79, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17556745, Length=156, Percent_Identity=39.7435897435897, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17533571, Length=152, Percent_Identity=40.7894736842105, Blast_Score=104, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17506493, Length=157, Percent_Identity=29.2993630573248, Blast_Score=73, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17557151, Length=165, Percent_Identity=29.6969696969697, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6322359, Length=145, Percent_Identity=42.7586206896552, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6323098, Length=154, Percent_Identity=38.961038961039, Blast_Score=113, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6324707, Length=145, Percent_Identity=35.1724137931034, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6320593, Length=145, Percent_Identity=35.1724137931034, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6323320, Length=170, Percent_Identity=28.8235294117647, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24582462, Length=684, Percent_Identity=24.2690058479532, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI221458488, Length=271, Percent_Identity=32.4723247232472, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI78706572, Length=132, Percent_Identity=33.3333333333333, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24585711, Length=149, Percent_Identity=30.8724832214765, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24585713, Length=149, Percent_Identity=30.8724832214765, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24585709, Length=149, Percent_Identity=30.8724832214765, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28574573, Length=141, Percent_Identity=31.9148936170213, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR004540
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 71519; Mature: 71519

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYYRTVGIFAHIDSGKTTLTERILYESGKISAVGSIEDGNTESDSLQEEIERGISIRTT
CCCEEEEEEEEEECCCCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHCCCEEEEE
FHSLPWQTDFGTFQIQLVDTPGHIDFRNQVTDLLPAMETAVVVLEAGTVVQSQARLVIEE
ECCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHH
LRKASVPIVFFINKLDRFGDEYLDTLVSLEEILGVAPVCLFQKTESGALEYYLNHSHSFA
HHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCHHHH
KKVDEELLSWNDELLISSWSQSSSSADFARIGLKDGPKSGKLFPVYGGSAKTGEGVRELL
HHHHHHHHCCCCCEEEEECCCCCCCCCEEEECCCCCCCCCCEEEEECCCCCCCHHHHHHH
DLLLWTNPKVPTDIIPLAYPLLVLSRRMAEGIGPYAVVYPLKDLSELDLLSMTETFLQSV
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHC
PLPPNASNAGLFQFIDPETEETTQEIHKGKLVLLKHNERFLLYPGEPVALRNVQGIGNEN
CCCCCCCCCCEEEEECCCCHHHHHHHHCCCEEEEEECCEEEEECCCCEEEECCCCCCCCC
QKDQRSPSPFSLVIEPEESSDKEFWLCRLEELVWEDPGYQTAIKEDTGQIVLFGRGELHL
CCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEECCEEEE
EIGIKRISGKTEKKLTFSSINIAKLELFKKMSHKVALEHRAFEDQKSSGTLIAVLEDTAD
EEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCC
FSKQIAFEVSLPEEVKNSIEMSFKEACLHGFYGEEVCGLRLRILSYEMPKGDLQTTLTLL
CCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCHHHCEEEEEEEECCCCCHHHHHHHHH
KVAILAGVKECFPSNTYLVGPLTEIEVMVDSDHLGVVLSDLSRRDAKVVSIIEAVAGKSH
HHHHHHHHHHHCCCCCEEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
LKANAPAQNLLGFSGALRNMTKGIGISWERTAFTSEFYAVLKE
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKYYRTVGIFAHIDSGKTTLTERILYESGKISAVGSIEDGNTESDSLQEEIERGISIRTT
CCCEEEEEEEEEECCCCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHCCCEEEEE
FHSLPWQTDFGTFQIQLVDTPGHIDFRNQVTDLLPAMETAVVVLEAGTVVQSQARLVIEE
ECCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHH
LRKASVPIVFFINKLDRFGDEYLDTLVSLEEILGVAPVCLFQKTESGALEYYLNHSHSFA
HHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCHHHH
KKVDEELLSWNDELLISSWSQSSSSADFARIGLKDGPKSGKLFPVYGGSAKTGEGVRELL
HHHHHHHHCCCCCEEEEECCCCCCCCCEEEECCCCCCCCCCEEEEECCCCCCCHHHHHHH
DLLLWTNPKVPTDIIPLAYPLLVLSRRMAEGIGPYAVVYPLKDLSELDLLSMTETFLQSV
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHC
PLPPNASNAGLFQFIDPETEETTQEIHKGKLVLLKHNERFLLYPGEPVALRNVQGIGNEN
CCCCCCCCCCEEEEECCCCHHHHHHHHCCCEEEEEECCEEEEECCCCEEEECCCCCCCCC
QKDQRSPSPFSLVIEPEESSDKEFWLCRLEELVWEDPGYQTAIKEDTGQIVLFGRGELHL
CCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEECCEEEE
EIGIKRISGKTEKKLTFSSINIAKLELFKKMSHKVALEHRAFEDQKSSGTLIAVLEDTAD
EEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCC
FSKQIAFEVSLPEEVKNSIEMSFKEACLHGFYGEEVCGLRLRILSYEMPKGDLQTTLTLL
CCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCHHHCEEEEEEEECCCCCHHHHHHHHH
KVAILAGVKECFPSNTYLVGPLTEIEVMVDSDHLGVVLSDLSRRDAKVVSIIEAVAGKSH
HHHHHHHHHHHCCCCCEEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
LKANAPAQNLLGFSGALRNMTKGIGISWERTAFTSEFYAVLKE
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]