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The map label for this gene is tuf

Identifier: 183221349

GI number: 183221349

Start: 2042033

End: 2043238

Strand: Reverse

Name: tuf

Synonym: LEPBI_I1966

Alternate gene names: 183221349

Gene position: 2043238-2042033 (Counterclockwise)

Preceding gene: 183221350

Following gene: 183221348

Centisome position: 56.76

GC content: 42.7

Gene sequence:

>1206_bases
ATGGCTAAAGAAAAATTTGACCGTTCAAAACCACACTTAAACATCGGAACAATTGGTCACGTTGACCACGGTAAAACAAC
CCTAACGGCAGCAATCACAACAACGCTTGCGAAGTTAGTAGGTGGAAAAAACAAAGCGATTGCTTATGACCAAATCGACA
ACGCGCCAGAAGAAAAGGCTCGTGGGATTACTATTGCAACGTCTCACCAGGAATATGAAACTCCTAACCGTCACTATGCA
CACGTAGATTGTCCAGGTCACGCTGACTATGTAAAAAACATGATTACTGGTGCTGCTCAGATGGACGCTGCGATTCTCGT
AGTATCTGCCACTGACGGTGCTATGCCACAAACGAAAGAACACATCCTACTTGCTCGCCAAGTTGGTGTTCCTTACATCG
TGGTTTACCTCAACAAAGCTGATATGTTAGCTGCTGATGAAAGAGACGATATGGTTGAAATGGTAAAAGAGGAAATCAAA
GACCTCCTTAACAAATACAACTTTCCAGGTGATAAAACACCTTTCATCTCTGGTTCTGCATTAAAAGCTCTTGAGGGTGA
AGATTCTGACCTAGGTATGAAATCCATTTTAAAACTAATGGAAGCAGTTGATACTTACGTTCCAAACCCTACTCGTATTG
TTGATAAACCTTTCCTAATGCCAGTTGAGGACGTGTTCTCAATTACTGGTCGTGGAACTGTTGCAACAGGTCGTGTAGAG
CAAGGTGTTCTTAAGATCAACGACGAGATCGAGATTGTTGGTATTCGTGATACTACAAAATCAGTTGTTACTGGTATTGA
AATGTTCCGTAAACTACTCGATCAAGCAGAAGCTGGAGACAATATTGGTGCTCTTCTTCGCGGAACGAAAAAAGAAGACA
TCGAAAGAGGTCAAGTTCTTGCGAAACCGGGTACAATCACTCCACACAGAAAGTTTAAGGCAGAAGTTTACGTTCTTACA
AAAGACGAAGGTGGACGTCATACTCCATTCTTTAACAACTACCGCCCTCAATTCTACTTCAGAACTACTGACATCACTGG
TGTTTGTAACCTTCCTGGTGGAATGGAGATGGTAATGCCAGGTGATAACGTTACGATGTCAATCGAACTAATTCACCCAA
TTGCTATGGACCAAGGTTTGAAGTTCGCTATCCGTGAGGGTGGAAGAACAATTGGTTCTGGTGTTGTTGCGGAGATCGTC
GAGTAA

Upstream 100 bases:

>100_bases
TGGGAAAGGACTGCTTTTACCTCTGAATTTTATGCAGTTCTAAAGGAGTAAAGAACCGAATATCGGGTCTTGCACCACGA
TTGAGGAGTAGATTCTAACA

Downstream 100 bases:

>100_bases
TACAATGGCTGGACAAAGAATTCGCGTTAAATTAAAAGCTTTCGATCATCGGTTGATTGACCAATCAACCTTTGAAATCG
TTGCGACTGCGAAGAGGACC

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MAKEKFDRSKPHLNIGTIGHVDHGKTTLTAAITTTLAKLVGGKNKAIAYDQIDNAPEEKARGITIATSHQEYETPNRHYA
HVDCPGHADYVKNMITGAAQMDAAILVVSATDGAMPQTKEHILLARQVGVPYIVVYLNKADMLAADERDDMVEMVKEEIK
DLLNKYNFPGDKTPFISGSALKALEGEDSDLGMKSILKLMEAVDTYVPNPTRIVDKPFLMPVEDVFSITGRGTVATGRVE
QGVLKINDEIEIVGIRDTTKSVVTGIEMFRKLLDQAEAGDNIGALLRGTKKEDIERGQVLAKPGTITPHRKFKAEVYVLT
KDEGGRHTPFFNNYRPQFYFRTTDITGVCNLPGGMEMVMPGDNVTMSIELIHPIAMDQGLKFAIREGGRTIGSGVVAEIV
E

Sequences:

>Translated_401_residues
MAKEKFDRSKPHLNIGTIGHVDHGKTTLTAAITTTLAKLVGGKNKAIAYDQIDNAPEEKARGITIATSHQEYETPNRHYA
HVDCPGHADYVKNMITGAAQMDAAILVVSATDGAMPQTKEHILLARQVGVPYIVVYLNKADMLAADERDDMVEMVKEEIK
DLLNKYNFPGDKTPFISGSALKALEGEDSDLGMKSILKLMEAVDTYVPNPTRIVDKPFLMPVEDVFSITGRGTVATGRVE
QGVLKINDEIEIVGIRDTTKSVVTGIEMFRKLLDQAEAGDNIGALLRGTKKEDIERGQVLAKPGTITPHRKFKAEVYVLT
KDEGGRHTPFFNNYRPQFYFRTTDITGVCNLPGGMEMVMPGDNVTMSIELIHPIAMDQGLKFAIREGGRTIGSGVVAEIV
E
>Mature_400_residues
AKEKFDRSKPHLNIGTIGHVDHGKTTLTAAITTTLAKLVGGKNKAIAYDQIDNAPEEKARGITIATSHQEYETPNRHYAH
VDCPGHADYVKNMITGAAQMDAAILVVSATDGAMPQTKEHILLARQVGVPYIVVYLNKADMLAADERDDMVEMVKEEIKD
LLNKYNFPGDKTPFISGSALKALEGEDSDLGMKSILKLMEAVDTYVPNPTRIVDKPFLMPVEDVFSITGRGTVATGRVEQ
GVLKINDEIEIVGIRDTTKSVVTGIEMFRKLLDQAEAGDNIGALLRGTKKEDIERGQVLAKPGTITPHRKFKAEVYVLTK
DEGGRHTPFFNNYRPQFYFRTTDITGVCNLPGGMEMVMPGDNVTMSIELIHPIAMDQGLKFAIREGGRTIGSGVVAEIVE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=395, Percent_Identity=56.7088607594937, Blast_Score=473, Evalue=1e-133,
Organism=Homo sapiens, GI4503475, Length=349, Percent_Identity=32.378223495702, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI223555963, Length=444, Percent_Identity=29.0540540540541, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI5729864, Length=444, Percent_Identity=29.0540540540541, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI4503471, Length=349, Percent_Identity=33.5243553008596, Blast_Score=155, Evalue=7e-38,
Organism=Homo sapiens, GI194018522, Length=334, Percent_Identity=31.1377245508982, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI194097354, Length=334, Percent_Identity=31.1377245508982, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI194018520, Length=334, Percent_Identity=31.1377245508982, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI54607086, Length=323, Percent_Identity=27.8637770897833, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI46094014, Length=334, Percent_Identity=30.2395209580838, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI4503507, Length=273, Percent_Identity=26.7399267399267, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI94966754, Length=139, Percent_Identity=34.5323741007194, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI157426893, Length=257, Percent_Identity=27.6264591439689, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1790412, Length=400, Percent_Identity=70.75, Blast_Score=588, Evalue=1e-169,
Organism=Escherichia coli, GI1789737, Length=400, Percent_Identity=70.75, Blast_Score=587, Evalue=1e-169,
Organism=Escherichia coli, GI2367247, Length=339, Percent_Identity=30.0884955752212, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI48994988, Length=275, Percent_Identity=30.5454545454545, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1789108, Length=322, Percent_Identity=27.3291925465839, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1788922, Length=155, Percent_Identity=31.6129032258064, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=407, Percent_Identity=51.1056511056511, Blast_Score=385, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI25141371, Length=392, Percent_Identity=43.1122448979592, Blast_Score=325, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI17552884, Length=355, Percent_Identity=31.830985915493, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17569207, Length=355, Percent_Identity=31.830985915493, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI115532067, Length=437, Percent_Identity=27.9176201372998, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI115532065, Length=437, Percent_Identity=28.1464530892449, Blast_Score=128, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI32566303, Length=344, Percent_Identity=29.6511627906977, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI32566629, Length=342, Percent_Identity=27.4853801169591, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17506081, Length=278, Percent_Identity=30.2158273381295, Blast_Score=113, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI32566301, Length=176, Percent_Identity=32.9545454545455, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17557151, Length=238, Percent_Identity=28.1512605042017, Blast_Score=86, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6324761, Length=401, Percent_Identity=61.8453865336658, Blast_Score=502, Evalue=1e-143,
Organism=Saccharomyces cerevisiae, GI6325337, Length=350, Percent_Identity=32.8571428571429, Blast_Score=152, Evalue=8e-38,
Organism=Saccharomyces cerevisiae, GI6319594, Length=350, Percent_Identity=32.8571428571429, Blast_Score=152, Evalue=8e-38,
Organism=Saccharomyces cerevisiae, GI6322937, Length=351, Percent_Identity=25.9259259259259, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6320377, Length=347, Percent_Identity=27.6657060518732, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320863, Length=275, Percent_Identity=27.6363636363636, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323320, Length=246, Percent_Identity=28.4552845528455, Blast_Score=73, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6324550, Length=237, Percent_Identity=29.957805907173, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323098, Length=148, Percent_Identity=30.4054054054054, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI281363316, Length=394, Percent_Identity=55.3299492385787, Blast_Score=459, Evalue=1e-129,
Organism=Drosophila melanogaster, GI17864358, Length=394, Percent_Identity=55.3299492385787, Blast_Score=459, Evalue=1e-129,
Organism=Drosophila melanogaster, GI19921738, Length=391, Percent_Identity=45.2685421994885, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI45553807, Length=456, Percent_Identity=27.6315789473684, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI45553816, Length=456, Percent_Identity=27.6315789473684, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24651721, Length=456, Percent_Identity=27.6315789473684, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI17864154, Length=456, Percent_Identity=27.6315789473684, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24652838, Length=456, Percent_Identity=27.4122807017544, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI17137572, Length=456, Percent_Identity=27.4122807017544, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI45550900, Length=344, Percent_Identity=30.2325581395349, Blast_Score=135, Evalue=7e-32,
Organism=Drosophila melanogaster, GI19922690, Length=307, Percent_Identity=30.9446254071661, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI17137380, Length=350, Percent_Identity=29.4285714285714, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24647048, Length=274, Percent_Identity=27.007299270073, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24647046, Length=274, Percent_Identity=27.007299270073, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_LEPBA (B0SAF6)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001962997.1
- ProteinModelPortal:   B0SAF6
- SMR:   B0SAF6
- GeneID:   6389676
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_1915
- HOGENOM:   HBG307581
- OMA:   FEGQVYV
- ProtClustDB:   PRK00049
- BioCyc:   LBIF355278:LBF_1915-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 43926; Mature: 43795

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKFDRSKPHLNIGTIGHVDHGKTTLTAAITTTLAKLVGGKNKAIAYDQIDNAPEEKA
CCCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEHHHCCCCCHHHH
RGITIATSHQEYETPNRHYAHVDCPGHADYVKNMITGAAQMDAAILVVSATDGAMPQTKE
CCEEEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHCHHHCCEEEEEEEECCCCCCCCHH
HILLARQVGVPYIVVYLNKADMLAADERDDMVEMVKEEIKDLLNKYNFPGDKTPFISGSA
HEEEEHHCCCCEEEEEECCCCCEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCH
LKALEGEDSDLGMKSILKLMEAVDTYVPNPTRIVDKPFLMPVEDVFSITGRGTVATGRVE
HHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHCCCCEEEECCHH
QGVLKINDEIEIVGIRDTTKSVVTGIEMFRKLLDQAEAGDNIGALLRGTKKEDIERGQVL
CCEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHCCCEEE
AKPGTITPHRKFKAEVYVLTKDEGGRHTPFFNNYRPQFYFRTTDITGVCNLPGGMEMVMP
ECCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCEEECCCCCCEEEEC
GDNVTMSIELIHPIAMDQGLKFAIREGGRTIGSGVVAEIVE
CCCEEEEEEEECCHHHCCCCEEEECCCCCCHHCCHHHHHCC
>Mature Secondary Structure 
AKEKFDRSKPHLNIGTIGHVDHGKTTLTAAITTTLAKLVGGKNKAIAYDQIDNAPEEKA
CCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEHHHCCCCCHHHH
RGITIATSHQEYETPNRHYAHVDCPGHADYVKNMITGAAQMDAAILVVSATDGAMPQTKE
CCEEEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHCHHHCCEEEEEEEECCCCCCCCHH
HILLARQVGVPYIVVYLNKADMLAADERDDMVEMVKEEIKDLLNKYNFPGDKTPFISGSA
HEEEEHHCCCCEEEEEECCCCCEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCH
LKALEGEDSDLGMKSILKLMEAVDTYVPNPTRIVDKPFLMPVEDVFSITGRGTVATGRVE
HHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHCCCCEEEECCHH
QGVLKINDEIEIVGIRDTTKSVVTGIEMFRKLLDQAEAGDNIGALLRGTKKEDIERGQVL
CCEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHCCCEEE
AKPGTITPHRKFKAEVYVLTKDEGGRHTPFFNNYRPQFYFRTTDITGVCNLPGGMEMVMP
ECCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCEEECCCCCCEEEEC
GDNVTMSIELIHPIAMDQGLKFAIREGGRTIGSGVVAEIVE
CCCEEEEEEEECCHHHCCCCEEEECCCCCCHHCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA