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The map label for this gene is paaF [H]

Identifier: 183221172

GI number: 183221172

Start: 1864843

End: 1865601

Strand: Direct

Name: paaF [H]

Synonym: LEPBI_I1786

Alternate gene names: 183221172

Gene position: 1864843-1865601 (Clockwise)

Preceding gene: 183221171

Following gene: 183221177

Centisome position: 51.81

GC content: 37.29

Gene sequence:

>759_bases
ATGATTAGTTTCGAACTCAATGACGGAATCGGATTGATAAAACTTGCGATCAATGAAAAGAACAGTTTTTCCAATGAATC
ATTTTTGAATTTAAAAAAAGTAATCCAATCGGCAAAAGAATCCAATGCAAAAGTTGTCGTATTACGAAGTGAATCCAGTG
GTTCATTTTCATTGGGACTTGATCTCACAACTGTAAGCACAATGGATATGACAAAGGACCTTGCCCCATTTTTGGAATTA
TTTTACCACAATCTCACGGAACTTTACCAGCTCGAGGTGCCAACCATTGCAGAAGTTTCAGGACATGCTTTAGGTTATGG
TGCGATGCTTGCTTTGGTTTGTGACTATCGATTTGGAACTGCAGACATTCGTTTTGGATTACCGGAAGTAAAAATTGGAA
TCCAAGTCCCTTCCTTTGTGTATGCACTGATGGGGGAAGCTGTCGGTTACGACGTTGCCAAACGGCATGTTTTACTTGGT
GATGCGTTCAAAGCAAAAGAAATGCCTACTTTATTTGAAGAAATTACAGATTCAGAAGAAGATCTAAGGAAAAAATCAAA
ATCCTTACAAACAAAACTGAAAAAAAATTCTTACAGTGCGATGAAGGATACCAAAAAAGGAATCTTACATGTTCACAAAC
CACTTCTTGATTTAGTGAAAGATGATATGAAAAACACAATTAGTAGCATCCAATCTCCAGATGCAAAGGAAGGAATTTCC
GCATCAGTAGAAGTGAGAAGACCTGTGTTTACATCTTAA

Upstream 100 bases:

>100_bases
GAAGTGGTCAAAACCTATCTCCAAGAATTTTAATAAATTTTTTCTTGCGAAAAAAACCTAAGAATCTATTAGTTAGATAT
CTAACCAATTAGAGGGAATT

Downstream 100 bases:

>100_bases
ACATTCACACGATTCCCCTTCGATTCCGTATGTGTTTATAAATTTCATCGAGGATAGGATACAAAATATCCTCGTTTTCT
TTTGATATCCCCCAAGTCTG

Product: putative enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MISFELNDGIGLIKLAINEKNSFSNESFLNLKKVIQSAKESNAKVVVLRSESSGSFSLGLDLTTVSTMDMTKDLAPFLEL
FYHNLTELYQLEVPTIAEVSGHALGYGAMLALVCDYRFGTADIRFGLPEVKIGIQVPSFVYALMGEAVGYDVAKRHVLLG
DAFKAKEMPTLFEEITDSEEDLRKKSKSLQTKLKKNSYSAMKDTKKGILHVHKPLLDLVKDDMKNTISSIQSPDAKEGIS
ASVEVRRPVFTS

Sequences:

>Translated_252_residues
MISFELNDGIGLIKLAINEKNSFSNESFLNLKKVIQSAKESNAKVVVLRSESSGSFSLGLDLTTVSTMDMTKDLAPFLEL
FYHNLTELYQLEVPTIAEVSGHALGYGAMLALVCDYRFGTADIRFGLPEVKIGIQVPSFVYALMGEAVGYDVAKRHVLLG
DAFKAKEMPTLFEEITDSEEDLRKKSKSLQTKLKKNSYSAMKDTKKGILHVHKPLLDLVKDDMKNTISSIQSPDAKEGIS
ASVEVRRPVFTS
>Mature_252_residues
MISFELNDGIGLIKLAINEKNSFSNESFLNLKKVIQSAKESNAKVVVLRSESSGSFSLGLDLTTVSTMDMTKDLAPFLEL
FYHNLTELYQLEVPTIAEVSGHALGYGAMLALVCDYRFGTADIRFGLPEVKIGIQVPSFVYALMGEAVGYDVAKRHVLLG
DAFKAKEMPTLFEEITDSEEDLRKKSKSLQTKLKKNSYSAMKDTKKGILHVHKPLLDLVKDDMKNTISSIQSPDAKEGIS
ASVEVRRPVFTS

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=257, Percent_Identity=25.2918287937743, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787659, Length=235, Percent_Identity=25.9574468085106, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790281, Length=138, Percent_Identity=35.5072463768116, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI25145438, Length=247, Percent_Identity=25.5060728744939, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19921018, Length=193, Percent_Identity=28.4974093264249, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24583165, Length=253, Percent_Identity=25.296442687747, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24653139, Length=126, Percent_Identity=38.0952380952381, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI45550169, Length=256, Percent_Identity=22.65625, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27847; Mature: 27847

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISFELNDGIGLIKLAINEKNSFSNESFLNLKKVIQSAKESNAKVVVLRSESSGSFSLGL
CEEEEECCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEE
DLTTVSTMDMTKDLAPFLELFYHNLTELYQLEVPTIAEVSGHALGYGAMLALVCDYRFGT
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHCCCCC
ADIRFGLPEVKIGIQVPSFVYALMGEAVGYDVAKRHVLLGDAFKAKEMPTLFEEITDSEE
CEEEECCCCEEEEEECHHHHHHHHHHHHCHHHHHHEEEECCCHHHCCCHHHHHHHCCCHH
DLRKKSKSLQTKLKKNSYSAMKDTKKGILHVHKPLLDLVKDDMKNTISSIQSPDAKEGIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCC
ASVEVRRPVFTS
CCEEECCCCCCC
>Mature Secondary Structure
MISFELNDGIGLIKLAINEKNSFSNESFLNLKKVIQSAKESNAKVVVLRSESSGSFSLGL
CEEEEECCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEE
DLTTVSTMDMTKDLAPFLELFYHNLTELYQLEVPTIAEVSGHALGYGAMLALVCDYRFGT
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHCCCCC
ADIRFGLPEVKIGIQVPSFVYALMGEAVGYDVAKRHVLLGDAFKAKEMPTLFEEITDSEE
CEEEECCCCEEEEEECHHHHHHHHHHHHCHHHHHHEEEECCCHHHCCCHHHHHHHCCCHH
DLRKKSKSLQTKLKKNSYSAMKDTKKGILHVHKPLLDLVKDDMKNTISSIQSPDAKEGIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCC
ASVEVRRPVFTS
CCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]