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The map label for this gene is cpsA1 [H]

Identifier: 183221171

GI number: 183221171

Start: 1863597

End: 1864775

Strand: Direct

Name: cpsA1 [H]

Synonym: LEPBI_I1785

Alternate gene names: 183221171

Gene position: 1863597-1864775 (Clockwise)

Preceding gene: 183221170

Following gene: 183221172

Centisome position: 51.77

GC content: 41.14

Gene sequence:

>1179_bases
ATGAAATCCTTACCCACACATAGAAAAGATGAAATGGTTCGTTACCGGAGAACCTTTCATCAAAACCCAGAACTTAAATA
CGAAGAAAAAGAAACAGCAAGGTTTGCCAAAGAACATCTAGAATCTCTTGGTTTCCAAGTAGAAGATGGAATCGCAGAAA
CAGGCCTAGTTGCCTTATTTGATTCAGGAATTCCAGGCAAAACCATTCTCGTTCGTGCTGACATGGATGCCCTACCGATC
CACGAAGAAAATTCCCATACTTACAAAAGCCGAAATGAAGGGAAAATGCATGCATGTGGACATGACGGACATACGAGCAT
TTTACTGGCGTTATCCTCTGATCTAAAAATAGATTTCAAAAGTTTTGTTCCCAAGGGAAAAGTTTTACTTTGTTTCCAAC
CAGCAGAAGAAGGTGGATCTGGTGCCGACAGAATGATTGAATCTGGAATTCTAGATCGATACCATGTTGATGCTGTATTT
GCCTTACATGTATGGAACCATATTCCATTAGGGAAAGTGGGTGTGGTAAATGGAACAATGATGGCATCGGTTGATGAGTT
TAAAATCACAATCCAAGGAACTTCTGGCCATGGAGCCATGCCCCAACATACAGTTGATCCAATTGTTGTTGGAAGTCATT
TGGTTGCCGCATTGCAAACGTTAGTATCAAGAAATGTGGATCCTCTGGAACCATGTGTAGTTACAGTTGGTTCGTTTCAT
TCCGGAAATGCATTTAACGTCATTCCAGAATCAGCCGTCCTTCATGGAACCGTCCGAACATATTCCAAATCAGTTTACGA
AATGATCCCAGAAAGAATGAGACAACTTACCAGCCAAGTTGGCGCTGGATTTGGTGCAAAAATCACACTTGATTACAAAC
GAATCGATAAACCCACAATCAATGATCCAGTTATGGCTGATGTGGTTCGAAAGGCTGCAAAAACAGTATTAGGAGACCAT
TGCCTTACGGAAGAAAATACGAGAACCATGGGTGGCGAAGATTTTTCTGCATTTCTTATGCAAAGACCTGGATGTTATTT
TTTTATTGGATCTCGAAACGAGGAGAAAGGATTTGTCCACCCTCACCACAGCTCATTTTTTGATTTTGATGAAGACGCAC
TTCCCATCGGTCTTTCGGTGATGAAGGAAGTGGTCAAAACCTATCTCCAAGAATTTTAA

Upstream 100 bases:

>100_bases
ATGAATTGGCGAAAAATTCACTGTTTGAAAAGTATGGACTGGATTTATATTCTATTTTAGAAGAAGTAGAAAAACGTGCC
AAACTCGAAACAGATAAATT

Downstream 100 bases:

>100_bases
TAAATTTTTTCTTGCGAAAAAAACCTAAGAATCTATTAGTTAGATATCTAACCAATTAGAGGGAATTATGATTAGTTTCG
AACTCAATGACGGAATCGGA

Product: putative N-acyl-L-amino acid amidohydrolase, M20D peptidase

Products: carboxylate; L-amino acid

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MKSLPTHRKDEMVRYRRTFHQNPELKYEEKETARFAKEHLESLGFQVEDGIAETGLVALFDSGIPGKTILVRADMDALPI
HEENSHTYKSRNEGKMHACGHDGHTSILLALSSDLKIDFKSFVPKGKVLLCFQPAEEGGSGADRMIESGILDRYHVDAVF
ALHVWNHIPLGKVGVVNGTMMASVDEFKITIQGTSGHGAMPQHTVDPIVVGSHLVAALQTLVSRNVDPLEPCVVTVGSFH
SGNAFNVIPESAVLHGTVRTYSKSVYEMIPERMRQLTSQVGAGFGAKITLDYKRIDKPTINDPVMADVVRKAAKTVLGDH
CLTEENTRTMGGEDFSAFLMQRPGCYFFIGSRNEEKGFVHPHHSSFFDFDEDALPIGLSVMKEVVKTYLQEF

Sequences:

>Translated_392_residues
MKSLPTHRKDEMVRYRRTFHQNPELKYEEKETARFAKEHLESLGFQVEDGIAETGLVALFDSGIPGKTILVRADMDALPI
HEENSHTYKSRNEGKMHACGHDGHTSILLALSSDLKIDFKSFVPKGKVLLCFQPAEEGGSGADRMIESGILDRYHVDAVF
ALHVWNHIPLGKVGVVNGTMMASVDEFKITIQGTSGHGAMPQHTVDPIVVGSHLVAALQTLVSRNVDPLEPCVVTVGSFH
SGNAFNVIPESAVLHGTVRTYSKSVYEMIPERMRQLTSQVGAGFGAKITLDYKRIDKPTINDPVMADVVRKAAKTVLGDH
CLTEENTRTMGGEDFSAFLMQRPGCYFFIGSRNEEKGFVHPHHSSFFDFDEDALPIGLSVMKEVVKTYLQEF
>Mature_392_residues
MKSLPTHRKDEMVRYRRTFHQNPELKYEEKETARFAKEHLESLGFQVEDGIAETGLVALFDSGIPGKTILVRADMDALPI
HEENSHTYKSRNEGKMHACGHDGHTSILLALSSDLKIDFKSFVPKGKVLLCFQPAEEGGSGADRMIESGILDRYHVDAVF
ALHVWNHIPLGKVGVVNGTMMASVDEFKITIQGTSGHGAMPQHTVDPIVVGSHLVAALQTLVSRNVDPLEPCVVTVGSFH
SGNAFNVIPESAVLHGTVRTYSKSVYEMIPERMRQLTSQVGAGFGAKITLDYKRIDKPTINDPVMADVVRKAAKTVLGDH
CLTEENTRTMGGEDFSAFLMQRPGCYFFIGSRNEEKGFVHPHHSSFFDFDEDALPIGLSVMKEVVKTYLQEF

Specific function: Can release basic, acidic, aromatic, and, to a lesser extent, aliphatic amino acids [H]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=435, Percent_Identity=26.8965517241379, Blast_Score=115, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: 3.5.1.14

Molecular weight: Translated: 43429; Mature: 43429

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSLPTHRKDEMVRYRRTFHQNPELKYEEKETARFAKEHLESLGFQVEDGIAETGLVALF
CCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCCCCCEEEEE
DSGIPGKTILVRADMDALPIHEENSHTYKSRNEGKMHACGHDGHTSILLALSSDLKIDFK
CCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCEEEEEECCCCEEEHH
SFVPKGKVLLCFQPAEEGGSGADRMIESGILDRYHVDAVFALHVWNHIPLGKVGVVNGTM
HCCCCCCEEEEECCCCCCCCCHHHHHHHCCCCHHHHHHEEEEHHHCCCCCCCEEEECCCE
MASVDEFKITIQGTSGHGAMPQHTVDPIVVGSHLVAALQTLVSRNVDPLEPCVVTVGSFH
EEEEEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCC
SGNAFNVIPESAVLHGTVRTYSKSVYEMIPERMRQLTSQVGAGFGAKITLDYKRIDKPTI
CCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHCCCCCC
NDPVMADVVRKAAKTVLGDHCLTEENTRTMGGEDFSAFLMQRPGCYFFIGSRNEEKGFVH
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCCCC
PHHSSFFDFDEDALPIGLSVMKEVVKTYLQEF
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
>Mature Secondary Structure
MKSLPTHRKDEMVRYRRTFHQNPELKYEEKETARFAKEHLESLGFQVEDGIAETGLVALF
CCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCCCCCEEEEE
DSGIPGKTILVRADMDALPIHEENSHTYKSRNEGKMHACGHDGHTSILLALSSDLKIDFK
CCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCEEEEEECCCCEEEHH
SFVPKGKVLLCFQPAEEGGSGADRMIESGILDRYHVDAVFALHVWNHIPLGKVGVVNGTM
HCCCCCCEEEEECCCCCCCCCHHHHHHHCCCCHHHHHHEEEEHHHCCCCCCCEEEECCCE
MASVDEFKITIQGTSGHGAMPQHTVDPIVVGSHLVAALQTLVSRNVDPLEPCVVTVGSFH
EEEEEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCC
SGNAFNVIPESAVLHGTVRTYSKSVYEMIPERMRQLTSQVGAGFGAKITLDYKRIDKPTI
CCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHCCCCCC
NDPVMADVVRKAAKTVLGDHCLTEENTRTMGGEDFSAFLMQRPGCYFFIGSRNEEKGFVH
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCCCC
PHHSSFFDFDEDALPIGLSVMKEVVKTYLQEF
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-acyl-L-amino acid; H2O

Specific reaction: An N-acyl-L-amino acid + H2O = a carboxylate + an L-amino acid

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7559343; 11427726; 1597179 [H]