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The map label for this gene is proB [H]

Identifier: 183221163

GI number: 183221163

Start: 1854882

End: 1855745

Strand: Direct

Name: proB [H]

Synonym: LEPBI_I1777

Alternate gene names: 183221163

Gene position: 1854882-1855745 (Clockwise)

Preceding gene: 183221162

Following gene: 183221164

Centisome position: 51.53

GC content: 37.73

Gene sequence:

>864_bases
ATGAAAACACGTAAGGATTTTTTAGATTCCATCCAAAAATCAAAACTCATAGTCATTAAAATTGGTAGTGCGCGGGTTTC
TGGCGAAGAATCTAAAATTAATGATTTTTTATATGATTTGGTAGGTGACATTCGTTCCCTTCGTGACCAAGGAAAAGAAA
TCATTCTTGTATCCTCTGGTGCGATCGCACAAGGGAAAAAACTCTTGGTTCAAAAAAGTGGTTCTCACCTTCCCAATGGA
AAAACGTCACTTGCCGAGAAACAAGCGCTAGCTGCTATGGGGCAGAATAAACTTCTCAACCTATATGATAGTTTTTTTAG
TCGCGTGAATGTTCCCATTGCGCAAATTTTATTTGGACGAAAGGACTTAAACGAAGAAAATAGTTTCACAAATTTGAAAC
AAACATTCCGTCAACTTTTGGATTGGGGAATTTTACCCATTGTGAATGAAAACGATTCTGTTTCCACGGAAGAAATCAAT
TTAGGTGATAATGACATTCTTTCTGCCGTAGTTGCCTCTGTTGTAGGTGCAGACCTTCTTCTCATACTTACAGGTGTGGA
TGGTTTTTTAAATGGATCCGAAAAAATTGATTTGTTTACTGAAATCACAAAAGAAACAGAATCTTTGGCAACGGGTCCTT
CTGGTCCTGGTACGGGTGGAATGTTTACAAAAATCAACGCCGCAAAACTTTTGTTACCATTTGGAATCAAAACAGGAATT
GCAAATGGCGAAACAAAACATGCAATCAATCAATTTTTTTCAAGAGAATCGTTTGGGACCTTGGTTGCCAATGGAAGTTT
TCCACATAGAATTCCTTCCGCTTCTGAAATCCAATCACACTTTTTTTCCTATCAGGTGGAGTAA

Upstream 100 bases:

>100_bases
GGTGAAATCATTTGTATCTCAGCAGATAAAGAATTCAATTTGGAATATTTACTCGAACGTATCGACAAAGTATTTTTTAC
CGAAAAAGTAAAGTTAGTTT

Downstream 100 bases:

>100_bases
ACTATGACATTCGAAGCAACAGAATATGCAAAGTCCATTGCAACAAAGGCAAAAGAAGCAAGTCGTGCATTAAAAAGTCT
CACAACCCTACAAAAGAATT

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MKTRKDFLDSIQKSKLIVIKIGSARVSGEESKINDFLYDLVGDIRSLRDQGKEIILVSSGAIAQGKKLLVQKSGSHLPNG
KTSLAEKQALAAMGQNKLLNLYDSFFSRVNVPIAQILFGRKDLNEENSFTNLKQTFRQLLDWGILPIVNENDSVSTEEIN
LGDNDILSAVVASVVGADLLLILTGVDGFLNGSEKIDLFTEITKETESLATGPSGPGTGGMFTKINAAKLLLPFGIKTGI
ANGETKHAINQFFSRESFGTLVANGSFPHRIPSASEIQSHFFSYQVE

Sequences:

>Translated_287_residues
MKTRKDFLDSIQKSKLIVIKIGSARVSGEESKINDFLYDLVGDIRSLRDQGKEIILVSSGAIAQGKKLLVQKSGSHLPNG
KTSLAEKQALAAMGQNKLLNLYDSFFSRVNVPIAQILFGRKDLNEENSFTNLKQTFRQLLDWGILPIVNENDSVSTEEIN
LGDNDILSAVVASVVGADLLLILTGVDGFLNGSEKIDLFTEITKETESLATGPSGPGTGGMFTKINAAKLLLPFGIKTGI
ANGETKHAINQFFSRESFGTLVANGSFPHRIPSASEIQSHFFSYQVE
>Mature_287_residues
MKTRKDFLDSIQKSKLIVIKIGSARVSGEESKINDFLYDLVGDIRSLRDQGKEIILVSSGAIAQGKKLLVQKSGSHLPNG
KTSLAEKQALAAMGQNKLLNLYDSFFSRVNVPIAQILFGRKDLNEENSFTNLKQTFRQLLDWGILPIVNENDSVSTEEIN
LGDNDILSAVVASVVGADLLLILTGVDGFLNGSEKIDLFTEITKETESLATGPSGPGTGGMFTKINAAKLLLPFGIKTGI
ANGETKHAINQFFSRESFGTLVANGSFPHRIPSASEIQSHFFSYQVE

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate 5-kinase family [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=284, Percent_Identity=33.8028169014084, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI21361368, Length=286, Percent_Identity=33.5664335664336, Blast_Score=118, Evalue=6e-27,
Organism=Escherichia coli, GI1786437, Length=267, Percent_Identity=35.2059925093633, Blast_Score=152, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17569969, Length=282, Percent_Identity=31.2056737588652, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17569967, Length=282, Percent_Identity=31.2056737588652, Blast_Score=132, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6320506, Length=253, Percent_Identity=36.7588932806324, Blast_Score=137, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6321822, Length=240, Percent_Identity=37.5, Blast_Score=136, Evalue=4e-33,
Organism=Drosophila melanogaster, GI21357643, Length=282, Percent_Identity=32.6241134751773, Blast_Score=124, Evalue=6e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797 [H]

Pfam domain/function: PF00696 AA_kinase [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 31165; Mature: 31165

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTRKDFLDSIQKSKLIVIKIGSARVSGEESKINDFLYDLVGDIRSLRDQGKEIILVSSG
CCCHHHHHHHHHHCCEEEEEECCCEECCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECC
AIAQGKKLLVQKSGSHLPNGKTSLAEKQALAAMGQNKLLNLYDSFFSRVNVPIAQILFGR
CCCCCHHHEEECCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHCC
KDLNEENSFTNLKQTFRQLLDWGILPIVNENDSVSTEEINLGDNDILSAVVASVVGADLL
CCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCEEECCCCHHHHHHHHHHHHHHHHH
LILTGVDGFLNGSEKIDLFTEITKETESLATGPSGPGTGGMFTKINAAKLLLPFGIKTGI
HHHHCCHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECHHHHEEHHCHHHCC
ANGETKHAINQFFSRESFGTLVANGSFPHRIPSASEIQSHFFSYQVE
CCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MKTRKDFLDSIQKSKLIVIKIGSARVSGEESKINDFLYDLVGDIRSLRDQGKEIILVSSG
CCCHHHHHHHHHHCCEEEEEECCCEECCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECC
AIAQGKKLLVQKSGSHLPNGKTSLAEKQALAAMGQNKLLNLYDSFFSRVNVPIAQILFGR
CCCCCHHHEEECCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHCC
KDLNEENSFTNLKQTFRQLLDWGILPIVNENDSVSTEEINLGDNDILSAVVASVVGADLL
CCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCEEECCCCHHHHHHHHHHHHHHHHH
LILTGVDGFLNGSEKIDLFTEITKETESLATGPSGPGTGGMFTKINAAKLLLPFGIKTGI
HHHHCCHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECHHHHEEHHCHHHCC
ANGETKHAINQFFSRESFGTLVANGSFPHRIPSASEIQSHFFSYQVE
CCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA