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The map label for this gene is purK [H]

Identifier: 183221141

GI number: 183221141

Start: 1832747

End: 1833847

Strand: Direct

Name: purK [H]

Synonym: LEPBI_I1755

Alternate gene names: 183221141

Gene position: 1832747-1833847 (Clockwise)

Preceding gene: 183221140

Following gene: 183221149

Centisome position: 50.91

GC content: 37.42

Gene sequence:

>1101_bases
TTGATGAAAATAGGTGTTTTGGGATCTGGTCAGCTCGGCCAAATGATGTGTTTGGAAGCGATTCCACTTGGACATGATTT
CTATTGTTATTCGCCCGATCTAAATTCTCCTTCTGAAAAACTGGGGGCCAAGGCAACCATTGCATCTTATGATTCCATTC
AACAGTTAGAATCCTTCCTTCAAACGATCGATGTATTGAGTTTTGAGTTTGAAAACATACCAAAACCAACTCTTGAATTT
TTAAAAAACCAAAAATTGATCGCCATTTTCCCACCACCACAAGCACTCATCATTGCCCAAGATCGATTTTTAGAAAAAAA
TCATTTCCGAAAACTTGGATTTGAAACCGCAGAATTCTTTCACCTAACCAAAGCTTCTTCCAAATTTGAAATTTCGATCC
CATTTCCTTGGATCATCAAAACATTACGATTTGGATATGATGGGAAAGGCCAAGTCAAAGTGAATCATCAGTCAGAGTAT
GAATCCTTTTTACAATCGGCGTTTTTGAAAGATGAGTCTGAATATCTAATTGAAGAAGTGATTCCCTTTCAAAAGGAAAT
CAGTATTTTACTCACTCGTTTTCAAAATGGTGATATTGTCTGTTATGGTGCTGTCGAAAACGAACATAAGAATCATATTT
TAGATCTATCAATTTTCCCTGCCAGAATCCCTGTGGGTCTCAACTTAGAAGCAATTGAAATGGCGTCAAAATTAGCAGAA
TCTTTACACTATGTGGGAACGATGGGTGTTGAGTTTTTTTTGAAAAACAATCAATTGTATCTAAATGAATTTGCTCCAAG
GCCACACAATACAGGTCATTTCACCCAAGACTGCCAAAGTTTTTCTCAGTTCTATTTGCATGTACAAGCCATTACGGGAA
ACACTCCCCCAACGGATGTTAGGCCAAAGCCAACACTGATGAAAAATATCCTTGGGAATTCATTCGAAGAAAGCCTAGAA
ATTGCAAGTGAACTACAGAAAGATGATCGTTACCGGTTGCATTTATATGCAAAGGAAGAAGCAAAACCAGGAAGAAAAAT
GGGACATTTGAATTTTAAAGGAAGTTTAGAGGAAGTAAACCCCCTCTTTCATGACCTCTAA

Upstream 100 bases:

>100_bases
ATCCCTACTTGATTCCAGTCTTTCCAAAAAATTGGAAGACTATGCGGATCAAAATCGTGAACAAGCTCTTTCGAAAAATG
ACCAACTGGTTTAGGAATCA

Downstream 100 bases:

>100_bases
AATTTTTTAATCATTAGATTTAAGAAACAAGTTCCATTCCCCAATCCCTCACATTTCCAGCTCCCAAACACAATAAAAGG
TCTCCTTTGTTCAGATGAGA

Product: phosphoribosylaminoimidazole carboxylase ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MMKIGVLGSGQLGQMMCLEAIPLGHDFYCYSPDLNSPSEKLGAKATIASYDSIQQLESFLQTIDVLSFEFENIPKPTLEF
LKNQKLIAIFPPPQALIIAQDRFLEKNHFRKLGFETAEFFHLTKASSKFEISIPFPWIIKTLRFGYDGKGQVKVNHQSEY
ESFLQSAFLKDESEYLIEEVIPFQKEISILLTRFQNGDIVCYGAVENEHKNHILDLSIFPARIPVGLNLEAIEMASKLAE
SLHYVGTMGVEFFLKNNQLYLNEFAPRPHNTGHFTQDCQSFSQFYLHVQAITGNTPPTDVRPKPTLMKNILGNSFEESLE
IASELQKDDRYRLHLYAKEEAKPGRKMGHLNFKGSLEEVNPLFHDL

Sequences:

>Translated_366_residues
MMKIGVLGSGQLGQMMCLEAIPLGHDFYCYSPDLNSPSEKLGAKATIASYDSIQQLESFLQTIDVLSFEFENIPKPTLEF
LKNQKLIAIFPPPQALIIAQDRFLEKNHFRKLGFETAEFFHLTKASSKFEISIPFPWIIKTLRFGYDGKGQVKVNHQSEY
ESFLQSAFLKDESEYLIEEVIPFQKEISILLTRFQNGDIVCYGAVENEHKNHILDLSIFPARIPVGLNLEAIEMASKLAE
SLHYVGTMGVEFFLKNNQLYLNEFAPRPHNTGHFTQDCQSFSQFYLHVQAITGNTPPTDVRPKPTLMKNILGNSFEESLE
IASELQKDDRYRLHLYAKEEAKPGRKMGHLNFKGSLEEVNPLFHDL
>Mature_366_residues
MMKIGVLGSGQLGQMMCLEAIPLGHDFYCYSPDLNSPSEKLGAKATIASYDSIQQLESFLQTIDVLSFEFENIPKPTLEF
LKNQKLIAIFPPPQALIIAQDRFLEKNHFRKLGFETAEFFHLTKASSKFEISIPFPWIIKTLRFGYDGKGQVKVNHQSEY
ESFLQSAFLKDESEYLIEEVIPFQKEISILLTRFQNGDIVCYGAVENEHKNHILDLSIFPARIPVGLNLEAIEMASKLAE
SLHYVGTMGVEFFLKNNQLYLNEFAPRPHNTGHFTQDCQSFSQFYLHVQAITGNTPPTDVRPKPTLMKNILGNSFEESLE
IASELQKDDRYRLHLYAKEEAKPGRKMGHLNFKGSLEEVNPLFHDL

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=361, Percent_Identity=31.3019390581717, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1788155, Length=308, Percent_Identity=24.6753246753247, Blast_Score=73, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6324702, Length=368, Percent_Identity=32.0652173913043, Blast_Score=176, Evalue=7e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006140
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02826 2-Hacid_dh_C; PF02222 ATP-grasp [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 41761; Mature: 41761

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS50975 ATP_GRASP ; PS00065 D_2_HYDROXYACID_DH_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKIGVLGSGQLGQMMCLEAIPLGHDFYCYSPDLNSPSEKLGAKATIASYDSIQQLESFL
CEEEEECCCCCCCHHHHHHHCCCCCCEEEECCCCCCCHHHHCCCEEEHHHHHHHHHHHHH
QTIDVLSFEFENIPKPTLEFLKNQKLIAIFPPPQALIIAQDRFLEKNHFRKLGFETAEFF
HHHHHHHHHHHCCCHHHHHHHCCCCEEEEECCCCEEEEEHHHHHHHHHHHHHCCCHHHHE
HLTKASSKFEISIPFPWIIKTLRFGYDGKGQVKVNHQSEYESFLQSAFLKDESEYLIEEV
EEECCCCEEEEECCHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHCCHHHHHHHHH
IPFQKEISILLTRFQNGDIVCYGAVENEHKNHILDLSIFPARIPVGLNLEAIEMASKLAE
CCHHHHHHHHHHEECCCCEEEEECCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHH
SLHYVGTMGVEFFLKNNQLYLNEFAPRPHNTGHFTQDCQSFSQFYLHVQAITGNTPPTDV
HHHHHHCCCEEEEEECCEEEEEECCCCCCCCCCCHHHHHHHHHHEEEEEEEECCCCCCCC
RPKPTLMKNILGNSFEESLEIASELQKDDRYRLHLYAKEEAKPGRKMGHLNFKGSLEEVN
CCCHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHH
PLFHDL
HHHHCC
>Mature Secondary Structure
MMKIGVLGSGQLGQMMCLEAIPLGHDFYCYSPDLNSPSEKLGAKATIASYDSIQQLESFL
CEEEEECCCCCCCHHHHHHHCCCCCCEEEECCCCCCCHHHHCCCEEEHHHHHHHHHHHHH
QTIDVLSFEFENIPKPTLEFLKNQKLIAIFPPPQALIIAQDRFLEKNHFRKLGFETAEFF
HHHHHHHHHHHCCCHHHHHHHCCCCEEEEECCCCEEEEEHHHHHHHHHHHHHCCCHHHHE
HLTKASSKFEISIPFPWIIKTLRFGYDGKGQVKVNHQSEYESFLQSAFLKDESEYLIEEV
EEECCCCEEEEECCHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHCCHHHHHHHHH
IPFQKEISILLTRFQNGDIVCYGAVENEHKNHILDLSIFPARIPVGLNLEAIEMASKLAE
CCHHHHHHHHHHEECCCCEEEEECCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHH
SLHYVGTMGVEFFLKNNQLYLNEFAPRPHNTGHFTQDCQSFSQFYLHVQAITGNTPPTDV
HHHHHHCCCEEEEEECCEEEEEECCCCCCCCCCCHHHHHHHHHHEEEEEEEECCCCCCCC
RPKPTLMKNILGNSFEESLEIASELQKDDRYRLHLYAKEEAKPGRKMGHLNFKGSLEEVN
CCCHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHH
PLFHDL
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA