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The map label for this gene is folD

Identifier: 183221109

GI number: 183221109

Start: 1795335

End: 1796210

Strand: Direct

Name: folD

Synonym: LEPBI_I1723

Alternate gene names: 183221109

Gene position: 1795335-1796210 (Clockwise)

Preceding gene: 183221106

Following gene: 183221110

Centisome position: 49.87

GC content: 41.1

Gene sequence:

>876_bases
ATGAAATCTAGCATCCTATTAGACGGTAAAGCGATTTCCGAAAAAATCCGAAATCGAATCCAAGAAACATTAGCAAAAGC
AAAAGCCGAAGGTAAGGGAATTCCCACTTTGGCAACCATCCTTGTTGGGAATAACCCTGCATCGGAAACCTATGTGAACA
TGAAGGTAAAAGCCTGTGAAAAAGTGGGTATGCATTCGCGTTACATACGTTTGAAGGAAGAAACCACTACCGAAGAACTC
TTAGGCGAAATTCGAAAACTCAATTTAGATCCGAGTATCAATGGAATCTTATTACAACATCCAGTCCCTCACCAAATCGA
TGAACGTAAGGCCTTTGATGAAATCGCATTAGAAAAAGATGTGGATGGGGTGACAACTATTTCATTTGGAAAATTGTCCA
TGAATAGCGAAGCCTATTATCCGTGCACTCCGTACGGTATGGTTCTACTCTTACAAGAATATGGAATCGATGTGACAGGA
AAACATGCAGTGGTTGTGGGAAGGTCTCCTATCCTTGGAAAACCAATGGCCATCATGTTGACCAATTTGAATGCTACTGT
GACACTTTGCCATTCGAAAACAGAAGATTTACCATCACTTGTCAAACAAGCCGATATCATTGTTGGTGCTGTTGGGAAAC
CGGAATTCATCAAAGCTGAATGGATGAAAGAGGGAGTCGTGATTTTGGATGCTGGGTACAATGTGGGCAATGTCGGAGAT
ATTGAAGTTTCTAAGGCCAAAGATAAATCCTCTTATTATACTCCAGTACCTGGTGGTGTGGGACCGATGACCATTTCTGT
TTTACTCTTACAAACGATGTATAGTTTTTTAGGTCAATTTTCGCCTAAGTTGGAGTCTCATGTCGCAAATCGTTAG

Upstream 100 bases:

>100_bases
TGAGAGGGACGGACGGGTCTCTTTTGGTTCTAAACTTGGAATCATAGATGGTTTTTTCTGTTTGCCAGTTCAATTTTCTC
TGAAATTTTGAACTTACAGA

Downstream 100 bases:

>100_bases
TTTTGACGAATGTTTTCAAACCGTTCCAAAATTGGATCCTCCAAGTGATTTAGATAGTTTTTGGAAATCTGGAATCGGTG
AACTGAAAAAAGTTCCTATC

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MKSSILLDGKAISEKIRNRIQETLAKAKAEGKGIPTLATILVGNNPASETYVNMKVKACEKVGMHSRYIRLKEETTTEEL
LGEIRKLNLDPSINGILLQHPVPHQIDERKAFDEIALEKDVDGVTTISFGKLSMNSEAYYPCTPYGMVLLLQEYGIDVTG
KHAVVVGRSPILGKPMAIMLTNLNATVTLCHSKTEDLPSLVKQADIIVGAVGKPEFIKAEWMKEGVVILDAGYNVGNVGD
IEVSKAKDKSSYYTPVPGGVGPMTISVLLLQTMYSFLGQFSPKLESHVANR

Sequences:

>Translated_291_residues
MKSSILLDGKAISEKIRNRIQETLAKAKAEGKGIPTLATILVGNNPASETYVNMKVKACEKVGMHSRYIRLKEETTTEEL
LGEIRKLNLDPSINGILLQHPVPHQIDERKAFDEIALEKDVDGVTTISFGKLSMNSEAYYPCTPYGMVLLLQEYGIDVTG
KHAVVVGRSPILGKPMAIMLTNLNATVTLCHSKTEDLPSLVKQADIIVGAVGKPEFIKAEWMKEGVVILDAGYNVGNVGD
IEVSKAKDKSSYYTPVPGGVGPMTISVLLLQTMYSFLGQFSPKLESHVANR
>Mature_291_residues
MKSSILLDGKAISEKIRNRIQETLAKAKAEGKGIPTLATILVGNNPASETYVNMKVKACEKVGMHSRYIRLKEETTTEEL
LGEIRKLNLDPSINGILLQHPVPHQIDERKAFDEIALEKDVDGVTTISFGKLSMNSEAYYPCTPYGMVLLLQEYGIDVTG
KHAVVVGRSPILGKPMAIMLTNLNATVTLCHSKTEDLPSLVKQADIIVGAVGKPEFIKAEWMKEGVVILDAGYNVGNVGD
IEVSKAKDKSSYYTPVPGGVGPMTISVLLLQTMYSFLGQFSPKLESHVANR

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=300, Percent_Identity=41.6666666666667, Blast_Score=230, Evalue=9e-61,
Organism=Homo sapiens, GI222418558, Length=291, Percent_Identity=37.4570446735395, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI94721354, Length=291, Percent_Identity=35.7388316151203, Blast_Score=179, Evalue=2e-45,
Organism=Homo sapiens, GI36796743, Length=210, Percent_Identity=29.5238095238095, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI1786741, Length=284, Percent_Identity=42.9577464788732, Blast_Score=230, Evalue=9e-62,
Organism=Caenorhabditis elegans, GI17568735, Length=283, Percent_Identity=37.4558303886926, Blast_Score=192, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6319558, Length=288, Percent_Identity=40.625, Blast_Score=220, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6321643, Length=289, Percent_Identity=40.1384083044983, Blast_Score=213, Evalue=3e-56,
Organism=Saccharomyces cerevisiae, GI6322933, Length=307, Percent_Identity=27.3615635179153, Blast_Score=80, Evalue=3e-16,
Organism=Drosophila melanogaster, GI17136816, Length=300, Percent_Identity=40.3333333333333, Blast_Score=218, Evalue=3e-57,
Organism=Drosophila melanogaster, GI17136818, Length=300, Percent_Identity=40.3333333333333, Blast_Score=218, Evalue=3e-57,
Organism=Drosophila melanogaster, GI62472483, Length=289, Percent_Identity=37.3702422145329, Blast_Score=207, Evalue=6e-54,
Organism=Drosophila melanogaster, GI45551871, Length=289, Percent_Identity=37.3702422145329, Blast_Score=207, Evalue=6e-54,
Organism=Drosophila melanogaster, GI24645718, Length=289, Percent_Identity=37.3702422145329, Blast_Score=207, Evalue=8e-54,
Organism=Drosophila melanogaster, GI17137370, Length=289, Percent_Identity=37.3702422145329, Blast_Score=207, Evalue=8e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_LEPBA (B0S8U6)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001962755.1
- ProteinModelPortal:   B0S8U6
- SMR:   B0S8U6
- GeneID:   6387299
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_1670
- HOGENOM:   HBG328751
- OMA:   GPMTINT
- ProtClustDB:   PRK14177
- BioCyc:   LBIF355278:LBF_1670-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 31729; Mature: 31729

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSSILLDGKAISEKIRNRIQETLAKAKAEGKGIPTLATILVGNNPASETYVNMKVKACE
CCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEEEEHHHHH
KVGMHSRYIRLKEETTTEELLGEIRKLNLDPSINGILLQHPVPHQIDERKAFDEIALEKD
HCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCC
VDGVTTISFGKLSMNSEAYYPCTPYGMVLLLQEYGIDVTGKHAVVVGRSPILGKPMAIML
CCCEEEEEECEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCEEEE
TNLNATVTLCHSKTEDLPSLVKQADIIVGAVGKPEFIKAEWMKEGVVILDAGYNVGNVGD
ECCCEEEEEECCCCHHHHHHHHHHCEEEECCCCCCHHHHHHHHCCEEEEECCCCCCCCCC
IEVSKAKDKSSYYTPVPGGVGPMTISVLLLQTMYSFLGQFSPKLESHVANR
EEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCC
>Mature Secondary Structure
MKSSILLDGKAISEKIRNRIQETLAKAKAEGKGIPTLATILVGNNPASETYVNMKVKACE
CCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEEEEHHHHH
KVGMHSRYIRLKEETTTEELLGEIRKLNLDPSINGILLQHPVPHQIDERKAFDEIALEKD
HCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCC
VDGVTTISFGKLSMNSEAYYPCTPYGMVLLLQEYGIDVTGKHAVVVGRSPILGKPMAIML
CCCEEEEEECEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCEEEE
TNLNATVTLCHSKTEDLPSLVKQADIIVGAVGKPEFIKAEWMKEGVVILDAGYNVGNVGD
ECCCEEEEEECCCCHHHHHHHHHHCEEEECCCCCCHHHHHHHHCCEEEEECCCCCCCCCC
IEVSKAKDKSSYYTPVPGGVGPMTISVLLLQTMYSFLGQFSPKLESHVANR
EEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA