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The map label for this gene is eno

Identifier: 183221090

GI number: 183221090

Start: 1777645

End: 1778946

Strand: Direct

Name: eno

Synonym: LEPBI_I1704

Alternate gene names: 183221090

Gene position: 1777645-1778946 (Clockwise)

Preceding gene: 183221085

Following gene: 183221091

Centisome position: 49.38

GC content: 44.78

Gene sequence:

>1302_bases
ATGTCCCAAAAAGATAGCATTCGTTCCGTCAGAGCCCGTGAAATTATGGATTCCAGAGGAAATCCAACCGTTGAAGTGGA
TGTCACTTTAGAAGACGGTTCTTTTGGTCGTGCGGCGGTTCCCTCTGGGGCATCCACTGGAGAACACGAAGCAGTCGAAC
TGCGTGACGGTGATAAAAAAAGGTACGGAGGAAAAGGAGTCCAAAAAGCTGTCGACAACGTGAATGCAAAAATCTCTAAA
TCCATCCTCGGGCTTTCCGCAACGGACCAACTCCAAATTGATGGTACGATGATCGCGCTAGATGGAACTGCCAATAAATC
CAAGTTAGGTGCAAATGCGATTTTAGGTGTTTCTATGGCAGTGGCAAAAGCAGCTGCAGTCCATGCAGGACTTCCACTAT
ACCGTTACATCGGGGGAACCTTCGCTCGTGAACTTCCTGTTCCTATGATGAATATCATCAACGGTGGTGCCCATGCGGAC
AATAATATCGACTTCCAAGAATTTATGATCCTACCTGTGTCGGCTCCAAACTTCAAAGAAGCTCTTCGGATGGGAGCAGA
AGTGTTCCATAGCCTTAAGACTGTTCTCAAAGGAAAAGGTTTGAACACCGCCGTTGGTGATGAAGGTGGATTTGCCCCAA
ACCTCACAAGTAATAGCGAAGCCATTGAAGTCATCCTCACTGCCATCGAAAAGGCAGGATACAAACCAGACCTCGATATC
AAGATCGGACTCGATTGTGCTGCCTCAGAATTCTATGATGAGAAAAAGAAGAAATACATCCTCAAAGCAGAGAAAAAACC
AGAAAAGACGGCAGAAGAACTCGTAGAATACTACTCAAATTTAGTGTCCAAGTATCCGATCATTACTATGGAAGACGGTC
TGGACGAAAACGATTGGTCAGGCTGGAAAAAACTTTCCGAAAAATTAGGGAAAAAGATCCAACTTGTGGGGGATGATTTG
TTTGTAACCAATATCAAAAAACTCGCACAAGGGATTGATAAAGGGATCGGTAACTCGATTCTCATCAAGGTAAACCAAAT
TGGAAGCCTTACCGAAACCCTAAGTGCCATTGAAATGGCCAAAAAAGCCCAGTACACGGCTGTTGTTTCCCACCGTTCAG
GAGAAACAGAAGATGCGACCATTTCGCACATTGCTGTCGCAACTAATTCTGGCCAAATCAAAACTGGTTCCTTAAGCCGA
ACCGACAGAATTGCAAAGTACAACGAACTCCTTCGCATCGAAGAAGAGTTGGGTAAAAATGCAACCTATAGCGGAGTGAA
TACGTTTTACAACCTGAGATAA

Upstream 100 bases:

>100_bases
ATTTTCCTGGAACGAGGCTCTTTGTCGAAAAAGTTTATTGTCAGAGGCTTAAAATTCCTAAGACTGGAAGGAAATTATCC
CTTAGGAGAAACAATTCCCG

Downstream 100 bases:

>100_bases
TATGACAGCAACCAAAGCCTCTCTTCTTTTAACCTATGTTTGTGCTTTTCTCTACTTGGGACTTTTGTCTGAGTCTGGGA
TGGCAGAACGATTGCGTTTG

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MSQKDSIRSVRAREIMDSRGNPTVEVDVTLEDGSFGRAAVPSGASTGEHEAVELRDGDKKRYGGKGVQKAVDNVNAKISK
SILGLSATDQLQIDGTMIALDGTANKSKLGANAILGVSMAVAKAAAVHAGLPLYRYIGGTFARELPVPMMNIINGGAHAD
NNIDFQEFMILPVSAPNFKEALRMGAEVFHSLKTVLKGKGLNTAVGDEGGFAPNLTSNSEAIEVILTAIEKAGYKPDLDI
KIGLDCAASEFYDEKKKKYILKAEKKPEKTAEELVEYYSNLVSKYPIITMEDGLDENDWSGWKKLSEKLGKKIQLVGDDL
FVTNIKKLAQGIDKGIGNSILIKVNQIGSLTETLSAIEMAKKAQYTAVVSHRSGETEDATISHIAVATNSGQIKTGSLSR
TDRIAKYNELLRIEEELGKNATYSGVNTFYNLR

Sequences:

>Translated_433_residues
MSQKDSIRSVRAREIMDSRGNPTVEVDVTLEDGSFGRAAVPSGASTGEHEAVELRDGDKKRYGGKGVQKAVDNVNAKISK
SILGLSATDQLQIDGTMIALDGTANKSKLGANAILGVSMAVAKAAAVHAGLPLYRYIGGTFARELPVPMMNIINGGAHAD
NNIDFQEFMILPVSAPNFKEALRMGAEVFHSLKTVLKGKGLNTAVGDEGGFAPNLTSNSEAIEVILTAIEKAGYKPDLDI
KIGLDCAASEFYDEKKKKYILKAEKKPEKTAEELVEYYSNLVSKYPIITMEDGLDENDWSGWKKLSEKLGKKIQLVGDDL
FVTNIKKLAQGIDKGIGNSILIKVNQIGSLTETLSAIEMAKKAQYTAVVSHRSGETEDATISHIAVATNSGQIKTGSLSR
TDRIAKYNELLRIEEELGKNATYSGVNTFYNLR
>Mature_432_residues
SQKDSIRSVRAREIMDSRGNPTVEVDVTLEDGSFGRAAVPSGASTGEHEAVELRDGDKKRYGGKGVQKAVDNVNAKISKS
ILGLSATDQLQIDGTMIALDGTANKSKLGANAILGVSMAVAKAAAVHAGLPLYRYIGGTFARELPVPMMNIINGGAHADN
NIDFQEFMILPVSAPNFKEALRMGAEVFHSLKTVLKGKGLNTAVGDEGGFAPNLTSNSEAIEVILTAIEKAGYKPDLDIK
IGLDCAASEFYDEKKKKYILKAEKKPEKTAEELVEYYSNLVSKYPIITMEDGLDENDWSGWKKLSEKLGKKIQLVGDDLF
VTNIKKLAQGIDKGIGNSILIKVNQIGSLTETLSAIEMAKKAQYTAVVSHRSGETEDATISHIAVATNSGQIKTGSLSRT
DRIAKYNELLRIEEELGKNATYSGVNTFYNLR

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=431, Percent_Identity=53.1322505800464, Blast_Score=440, Evalue=1e-123,
Organism=Homo sapiens, GI301897477, Length=429, Percent_Identity=53.8461538461538, Blast_Score=437, Evalue=1e-122,
Organism=Homo sapiens, GI301897469, Length=429, Percent_Identity=53.8461538461538, Blast_Score=437, Evalue=1e-122,
Organism=Homo sapiens, GI4503571, Length=431, Percent_Identity=52.9002320185615, Blast_Score=437, Evalue=1e-122,
Organism=Homo sapiens, GI301897479, Length=427, Percent_Identity=48.9461358313817, Blast_Score=382, Evalue=1e-106,
Organism=Homo sapiens, GI169201331, Length=353, Percent_Identity=26.3456090651558, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI169201757, Length=353, Percent_Identity=26.3456090651558, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI239744207, Length=353, Percent_Identity=26.3456090651558, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI1789141, Length=423, Percent_Identity=63.1205673758865, Blast_Score=513, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI71995829, Length=435, Percent_Identity=53.7931034482759, Blast_Score=442, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI17536383, Length=431, Percent_Identity=54.0603248259861, Blast_Score=441, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI32563855, Length=191, Percent_Identity=46.0732984293194, Blast_Score=179, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6323985, Length=435, Percent_Identity=49.8850574712644, Blast_Score=399, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6324974, Length=435, Percent_Identity=49.8850574712644, Blast_Score=398, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6324969, Length=435, Percent_Identity=49.8850574712644, Blast_Score=398, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6321693, Length=437, Percent_Identity=48.2837528604119, Blast_Score=390, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6321968, Length=437, Percent_Identity=48.0549199084668, Blast_Score=370, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24580918, Length=434, Percent_Identity=51.8433179723502, Blast_Score=407, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580916, Length=434, Percent_Identity=51.8433179723502, Blast_Score=407, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580920, Length=434, Percent_Identity=51.8433179723502, Blast_Score=407, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580914, Length=434, Percent_Identity=51.8433179723502, Blast_Score=407, Evalue=1e-114,
Organism=Drosophila melanogaster, GI281360527, Length=430, Percent_Identity=52.093023255814, Blast_Score=406, Evalue=1e-113,
Organism=Drosophila melanogaster, GI17137654, Length=430, Percent_Identity=52.093023255814, Blast_Score=406, Evalue=1e-113,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_LEPBA (B0S8S8)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001962737.1
- ProteinModelPortal:   B0S8S8
- SMR:   B0S8S8
- GeneID:   6387102
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_1652
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- ProtClustDB:   PRK00077
- BioCyc:   LBIF355278:LBF_1652-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 46737; Mature: 46606

Theoretical pI: Translated: 6.82; Mature: 6.82

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 208-208 ACT_SITE 343-343 BINDING 158-158 BINDING 167-167 BINDING 291-291 BINDING 318-318 BINDING 343-343 BINDING 394-394

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQKDSIRSVRAREIMDSRGNPTVEVDVTLEDGSFGRAAVPSGASTGEHEAVELRDGDKK
CCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCH
RYGGKGVQKAVDNVNAKISKSILGLSATDQLQIDGTMIALDGTANKSKLGANAILGVSMA
HCCCCHHHHHHHHCCHHHHHHHHCCCCCCEEEECCEEEEEECCCCCHHCCCHHHHHHHHH
VAKAAAVHAGLPLYRYIGGTFARELPVPMMNIINGGAHADNNIDFQEFMILPVSAPNFKE
HHHHHHHHCCCHHHHHHCHHHHHHCCCHHHHHHCCCCCCCCCCCHHHEEEEECCCCCHHH
ALRMGAEVFHSLKTVLKGKGLNTAVGDEGGFAPNLTSNSEAIEVILTAIEKAGYKPDLDI
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEE
KIGLDCAASEFYDEKKKKYILKAEKKPEKTAEELVEYYSNLVSKYPIITMEDGLDENDWS
EECCCHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCH
GWKKLSEKLGKKIQLVGDDLFVTNIKKLAQGIDKGIGNSILIKVNQIGSLTETLSAIEMA
HHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHH
KKAQYTAVVSHRSGETEDATISHIAVATNSGQIKTGSLSRTDRIAKYNELLRIEEELGKN
HHHHHHEEEECCCCCCCCCEEEEEEEEECCCCEEECCCCHHHHHHHHHHHHHHHHHHCCC
ATYSGVNTFYNLR
CCCCCCCCEEECC
>Mature Secondary Structure 
SQKDSIRSVRAREIMDSRGNPTVEVDVTLEDGSFGRAAVPSGASTGEHEAVELRDGDKK
CCHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCH
RYGGKGVQKAVDNVNAKISKSILGLSATDQLQIDGTMIALDGTANKSKLGANAILGVSMA
HCCCCHHHHHHHHCCHHHHHHHHCCCCCCEEEECCEEEEEECCCCCHHCCCHHHHHHHHH
VAKAAAVHAGLPLYRYIGGTFARELPVPMMNIINGGAHADNNIDFQEFMILPVSAPNFKE
HHHHHHHHCCCHHHHHHCHHHHHHCCCHHHHHHCCCCCCCCCCCHHHEEEEECCCCCHHH
ALRMGAEVFHSLKTVLKGKGLNTAVGDEGGFAPNLTSNSEAIEVILTAIEKAGYKPDLDI
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEE
KIGLDCAASEFYDEKKKKYILKAEKKPEKTAEELVEYYSNLVSKYPIITMEDGLDENDWS
EECCCHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCH
GWKKLSEKLGKKIQLVGDDLFVTNIKKLAQGIDKGIGNSILIKVNQIGSLTETLSAIEMA
HHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHH
KKAQYTAVVSHRSGETEDATISHIAVATNSGQIKTGSLSRTDRIAKYNELLRIEEELGKN
HHHHHHEEEECCCCCCCCCEEEEEEEEECCCCEEECCCCHHHHHHHHHHHHHHHHHHCCC
ATYSGVNTFYNLR
CCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA