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The map label for this gene is gspE [H]

Identifier: 183221063

GI number: 183221063

Start: 1747825

End: 1749501

Strand: Direct

Name: gspE [H]

Synonym: LEPBI_I1676

Alternate gene names: 183221063

Gene position: 1747825-1749501 (Clockwise)

Preceding gene: 183221062

Following gene: 183221064

Centisome position: 48.56

GC content: 41.03

Gene sequence:

>1677_bases
ATGAGAAAAAGTTTAGGGCAGATTCTTTTAGAAGATGGAATCCTTACCATTAAGGATTTAGAGGATATTTCCAAACAACA
GGAAAAAACAAATCTTCCACTCACTCATATCATCCAAAAAAAAGGACTCGCATCCGAAACAGACATTTTAAAGGCACTGG
CAAAACTCCATAGAATGGAATTTTACGACAAACTTGAGTTTGTTGCCAGTGATGAGATCTTTAGTAAGATCCCTTTGAAA
CTAGTCCAACGTTCCAAAATTGTTCCCTTCCTAGTCAAAGGAAAAAAAGTTTTTGTCGCCACAAGTGATCCAACGGACCT
CCATCCGATGGATGATATGCGATCCTTCTTAAAAGGATACGAAATTCAATTTGTTCTCGCAACCGAGAACGAAATCATGC
GCATCGTCCATTCTCAGTTTGATAAAACAACTGCAGAAGCAAAAGAAATGATGGATGAGATGGATGGAAGTTTCGGAGAT
CTCTCCGATGCCTTCGAATCCGACGCACTCGATCTCTCTAACGAAGCACCCATCATCAAGATGGTGAATGTGATTTTATC
CCAGGCTGTGTCCGAGCGCGCTTCCGATATCCACATCGAACCATTTGAAAAATCTGTCATCGTTCGGTATCGTGTGGACG
GTGTGTTACAAAAAGTTTTAAATCCACCTAAGTCCTATTTGGCGGGAATTTCCACTCGTATTAAAATTATGTCGAATCTC
AATATTGCGGAAAATAGGCTCCCGCAAGATGGTAGAATTAAACTAAGATTAGCTGGTAAAGACGTGGATGTCAGGGTTTC
CATCATCCCATGCCAATTCGGCGAACGAATTGTAATGAGGATTTTGAATAAAACGGACCAAAAGTATTCCATTGATACGA
TGGGTTTTAACCCTCAAATCTTAAAAGAATTCAAAGAACTCATTTATAAACCATACGGAATCATACTTGTGACGGGTCCT
ACGGGATCTGGTAAATCAACCACTCTCTATTCTGCTTTATCTGAGATCAATACAGAAGAACGAAATATCATCACCTGTGA
AGATCCAGTGGAATACCAAATGGATGGAATTTCCCAAATGCAGATGAACGATAAAATTGGACTTACATTTGCAGCAGGAC
TTCGTTCCATTTTACGACAAGATCCGGACGTAGTAATGGTGGGGGAGATCCGGGATGAGGAAACAGCAAGGATTGCCATC
CAAGCTTCCCTAACAGGTCACTTGGTATTTTCAACCTTACACACCAATGACGCATCCTCAGCAGTGACAAGGCTTGTGGA
TATGGGTATTGAACCATATCTCATCACGAGTTCTGTATTAGGTTTTATGGCACAAAGGCTTGTTCGTGTGATTTGTAAGG
ATTGTAAAACTTCCTACAAACCAACTGACAAAGATTTGGCGGGACTTGGAATCCAAAGAAAAGAACTAAAAAATGGGGTT
TTGTACCGCGGGAAAGGTTGTAGCTCTTGTCTCAATTCAGGTTACAAGGGACGAACTGGTCTATACGAACTCCTTACCAT
GAACGATGAAATCAAACGCGCTATTTTACAAGGTGCCGATGCCAATCGCATCAAAGAACTTGCCGTTAAAAATGGACTAT
CTGTCCTTCAAGAATATGGTAAGTACAAAGTGATCGAGGGAGTGACAACTCCTGAAGAAGTCCTTCGGGTATCTTAA

Upstream 100 bases:

>100_bases
CACCGCACATTCTGGACGATCGGGATAAATCGGATCGAATGACCATCCAAAAGAAAAATGAACAAGAAAGGATGGTGGAT
GAACGAGAAAAGAAACTGAG

Downstream 100 bases:

>100_bases
GTTTATGCCACTTTATACCTACGTTGCCTTTAATAAAAAGGGAAAAGAAGAAAAAAACATCATTGATGCTGTGAATTTGC
AAGCAGCACGGAACAAACTA

Product: general secretion pathway protein E

Products: NA

Alternate protein names: Type II traffic warden ATPase [H]

Number of amino acids: Translated: 558; Mature: 558

Protein sequence:

>558_residues
MRKSLGQILLEDGILTIKDLEDISKQQEKTNLPLTHIIQKKGLASETDILKALAKLHRMEFYDKLEFVASDEIFSKIPLK
LVQRSKIVPFLVKGKKVFVATSDPTDLHPMDDMRSFLKGYEIQFVLATENEIMRIVHSQFDKTTAEAKEMMDEMDGSFGD
LSDAFESDALDLSNEAPIIKMVNVILSQAVSERASDIHIEPFEKSVIVRYRVDGVLQKVLNPPKSYLAGISTRIKIMSNL
NIAENRLPQDGRIKLRLAGKDVDVRVSIIPCQFGERIVMRILNKTDQKYSIDTMGFNPQILKEFKELIYKPYGIILVTGP
TGSGKSTTLYSALSEINTEERNIITCEDPVEYQMDGISQMQMNDKIGLTFAAGLRSILRQDPDVVMVGEIRDEETARIAI
QASLTGHLVFSTLHTNDASSAVTRLVDMGIEPYLITSSVLGFMAQRLVRVICKDCKTSYKPTDKDLAGLGIQRKELKNGV
LYRGKGCSSCLNSGYKGRTGLYELLTMNDEIKRAILQGADANRIKELAVKNGLSVLQEYGKYKVIEGVTTPEEVLRVS

Sequences:

>Translated_558_residues
MRKSLGQILLEDGILTIKDLEDISKQQEKTNLPLTHIIQKKGLASETDILKALAKLHRMEFYDKLEFVASDEIFSKIPLK
LVQRSKIVPFLVKGKKVFVATSDPTDLHPMDDMRSFLKGYEIQFVLATENEIMRIVHSQFDKTTAEAKEMMDEMDGSFGD
LSDAFESDALDLSNEAPIIKMVNVILSQAVSERASDIHIEPFEKSVIVRYRVDGVLQKVLNPPKSYLAGISTRIKIMSNL
NIAENRLPQDGRIKLRLAGKDVDVRVSIIPCQFGERIVMRILNKTDQKYSIDTMGFNPQILKEFKELIYKPYGIILVTGP
TGSGKSTTLYSALSEINTEERNIITCEDPVEYQMDGISQMQMNDKIGLTFAAGLRSILRQDPDVVMVGEIRDEETARIAI
QASLTGHLVFSTLHTNDASSAVTRLVDMGIEPYLITSSVLGFMAQRLVRVICKDCKTSYKPTDKDLAGLGIQRKELKNGV
LYRGKGCSSCLNSGYKGRTGLYELLTMNDEIKRAILQGADANRIKELAVKNGLSVLQEYGKYKVIEGVTTPEEVLRVS
>Mature_558_residues
MRKSLGQILLEDGILTIKDLEDISKQQEKTNLPLTHIIQKKGLASETDILKALAKLHRMEFYDKLEFVASDEIFSKIPLK
LVQRSKIVPFLVKGKKVFVATSDPTDLHPMDDMRSFLKGYEIQFVLATENEIMRIVHSQFDKTTAEAKEMMDEMDGSFGD
LSDAFESDALDLSNEAPIIKMVNVILSQAVSERASDIHIEPFEKSVIVRYRVDGVLQKVLNPPKSYLAGISTRIKIMSNL
NIAENRLPQDGRIKLRLAGKDVDVRVSIIPCQFGERIVMRILNKTDQKYSIDTMGFNPQILKEFKELIYKPYGIILVTGP
TGSGKSTTLYSALSEINTEERNIITCEDPVEYQMDGISQMQMNDKIGLTFAAGLRSILRQDPDVVMVGEIRDEETARIAI
QASLTGHLVFSTLHTNDASSAVTRLVDMGIEPYLITSSVLGFMAQRLVRVICKDCKTSYKPTDKDLAGLGIQRKELKNGV
LYRGKGCSSCLNSGYKGRTGLYELLTMNDEIKRAILQGADANRIKELAVKNGLSVLQEYGKYKVIEGVTTPEEVLRVS

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of a variety of enzymes across the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=423, Percent_Identity=48.6997635933806, Blast_Score=406, Evalue=1e-114,
Organism=Escherichia coli, GI1786296, Length=386, Percent_Identity=40.6735751295337, Blast_Score=315, Evalue=4e-87,
Organism=Escherichia coli, GI87082188, Length=128, Percent_Identity=51.5625, Blast_Score=124, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 62418; Mature: 62418

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKSLGQILLEDGILTIKDLEDISKQQEKTNLPLTHIIQKKGLASETDILKALAKLHRME
CCCHHHHHHHHCCCEEEHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHH
FYDKLEFVASDEIFSKIPLKLVQRSKIVPFLVKGKKVFVATSDPTDLHPMDDMRSFLKGY
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCCCCCCHHHHHHHHCCC
EIQFVLATENEIMRIVHSQFDKTTAEAKEMMDEMDGSFGDLSDAFESDALDLSNEAPIIK
EEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCHHH
MVNVILSQAVSERASDIHIEPFEKSVIVRYRVDGVLQKVLNPPKSYLAGISTRIKIMSNL
HHHHHHHHHHHHHHCCCEECCCCCCEEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
NIAENRLPQDGRIKLRLAGKDVDVRVSIIPCQFGERIVMRILNKTDQKYSIDTMGFNPQI
CHHHHCCCCCCCEEEEEECCCEEEEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCHHH
LKEFKELIYKPYGIILVTGPTGSGKSTTLYSALSEINTEERNIITCEDPVEYQMDGISQM
HHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCEEECCCCCHHHHCCCHHH
QMNDKIGLTFAAGLRSILRQDPDVVMVGEIRDEETARIAIQASLTGHLVFSTLHTNDASS
HCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEECCEEEEEEEECCCCHHH
AVTRLVDMGIEPYLITSSVLGFMAQRLVRVICKDCKTSYKPTDKDLAGLGIQRKELKNGV
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCHHHHHCCC
LYRGKGCSSCLNSGYKGRTGLYELLTMNDEIKRAILQGADANRIKELAVKNGLSVLQEYG
EECCCCHHHHHCCCCCCCCCHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
KYKVIEGVTTPEEVLRVS
CEEEEECCCCHHHHHCCC
>Mature Secondary Structure
MRKSLGQILLEDGILTIKDLEDISKQQEKTNLPLTHIIQKKGLASETDILKALAKLHRME
CCCHHHHHHHHCCCEEEHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHH
FYDKLEFVASDEIFSKIPLKLVQRSKIVPFLVKGKKVFVATSDPTDLHPMDDMRSFLKGY
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCCCCCCHHHHHHHHCCC
EIQFVLATENEIMRIVHSQFDKTTAEAKEMMDEMDGSFGDLSDAFESDALDLSNEAPIIK
EEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCHHH
MVNVILSQAVSERASDIHIEPFEKSVIVRYRVDGVLQKVLNPPKSYLAGISTRIKIMSNL
HHHHHHHHHHHHHHCCCEECCCCCCEEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
NIAENRLPQDGRIKLRLAGKDVDVRVSIIPCQFGERIVMRILNKTDQKYSIDTMGFNPQI
CHHHHCCCCCCCEEEEEECCCEEEEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCHHH
LKEFKELIYKPYGIILVTGPTGSGKSTTLYSALSEINTEERNIITCEDPVEYQMDGISQM
HHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCEEECCCCCHHHHCCCHHH
QMNDKIGLTFAAGLRSILRQDPDVVMVGEIRDEETARIAIQASLTGHLVFSTLHTNDASS
HCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEECCEEEEEEEECCCCHHH
AVTRLVDMGIEPYLITSSVLGFMAQRLVRVICKDCKTSYKPTDKDLAGLGIQRKELKNGV
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCHHHHHCCC
LYRGKGCSSCLNSGYKGRTGLYELLTMNDEIKRAILQGADANRIKELAVKNGLSVLQEYG
EECCCCHHHHHCCCCCCCCCHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
KYKVIEGVTTPEEVLRVS
CEEEEECCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1588814; 10984043 [H]