Click here to switch to the map view.

The map label for this gene is gspD [H]

Identifier: 183221062

GI number: 183221062

Start: 1746065

End: 1747828

Strand: Direct

Name: gspD [H]

Synonym: LEPBI_I1675

Alternate gene names: 183221062

Gene position: 1746065-1747828 (Clockwise)

Preceding gene: 183221061

Following gene: 183221063

Centisome position: 48.51

GC content: 42.35

Gene sequence:

>1764_bases
ATGAGAAATCGACTTCCCTCAGTTGTACTTTGTATTTGCCTTTACTTAATTGTAACCCCCAACTTTTCACAAGAGAAAGG
GAAACCGAAAGCAAAAACCTCACAAGAACCAGCTAGTTTCACTGCGGATTGGAGGGATACAGAACTCAAAGACTTCCTTA
TGGGAATGAGTGCCATCATCAAAAAAAATATTCTGATCGATGATGCGGTGAAAGGCAAAAAGATCACAATCATTTCTCAA
AAACGAGTTCGGATTGAAGATGCCTTTGGATTTATGAAGTCGGTTTTGGAAACCCAAGGGTTTGGACTCATCGAAGAAAA
TGACCTCATTAAAGTTGTCAAAATCAAAGACGCACTCGCTAAGTCACAAATTGTTCGGATCGGAAAGGATCCAGTCTCTG
ATTCGGAAGTTGCCTTAAACAAAACTATCACTCAGATTGTTCCACTAGAGTTTTCGAATGCCATTGAACTTGAGCCCATC
TTGAAACGTGTCACGTCTCCCGATACCGATATCATCATTCCTAAAAACCAAAACACATTGATTTTTTCTGGTTCCACAGC
AGATATCAACAAATTACTCAAGTTAGTTGATAATTTGGATGTAAGAGCAGATGGACCCGGGTCCATTTCTTCGGCTGGTG
ACATCCATATTTATACTTTGGAATACAATGAAGCAGAAAAACTCGCAGCCATCCTTGTGAAATTGGATATGCCTGATGCG
CCTGTCGCTCCGACGACTCAAGGTGCACCTGGTGAAGCGGGGGCTGATGGAAAACCTGCCCCTCCACCACAGCCAACTGC
CCAAGCACCCAAGGTTCCAGGCAAACAAGATAAAATCAAAGCAGTGGCCCATAAAGAATCGAATTCACTCATTGTAACAG
CCACGCCACAAGAATGGGAAGAAATTAAAAAAATCATCAAAATTCTAGATACACCGAGAAAACAGGTGTTACTCGAAGTT
CTCATTGTGGAGCTTAGTTCGACTGATTTAAATGATTTTGGTATCGATTGGCGTTACCAAGAGCTTGCCTATGGACAGTT
TAACACAGGTCTTGCGGCACAAGGGGGAGTGATCGATAAAAATGGTCGTCCTACCAATGTGAACACACTATCTGGTTTTT
CACTTGGATTCATCCGCCGTGGTGGGCAACAAATCATTGGTATTTTGAATGCAAACTCTACCAATGAAAACTTCAATGTA
TTGTCGGCTCCGCAAATTTTGACCTTGGACAACCAAGAGGCAGAAATCAATGTGGGTCAAGACGTTCCCGTTCGTACCCA
AAATCGAAATGCGGGTCTTGGTGGAGACAACGCGGTAACGGTAGCCAACTTTGAATACCGTCCAACAGGGATTAAACTAA
AGTTTACCCCACATATCAATAAAAACAACCGCATCACTCTCGATTTGTACCAAGAAATCAAAAACGTCGCGGGGATTTCA
TCGGAAGCCACTGGTGGAAACCCAACGTTTAATAAACGTGATATCAAAACAACCATCGTTGTGGATAATATCCAAACCAT
TGTGATAGGAGGACTCCTCTCCAATGACAAACAAAAGAAAGTGCAAAAGATCCCCATTTTAGGGGAGATTCCCCTCCTTG
GGACTTTGTTTCGTCGTACCACAAACCAAAATCGCAAAACCAATTTGATGGTGTTTTTAACACCGCACATTCTGGACGAT
CGGGATAAATCGGATCGAATGACCATCCAAAAGAAAAATGAACAAGAAAGGATGGTGGATGAACGAGAAAAGAAACTGAG
ATGA

Upstream 100 bases:

>100_bases
TTGGAAATTTGGGGTTCGATCAAACAGGCTCCGAAAATTACAGTGGATTTAGAACGACAAGGCAAAATTATCACATATGA
ATTTATCATTCGGAATTAAA

Downstream 100 bases:

>100_bases
GAAAAAGTTTAGGGCAGATTCTTTTAGAAGATGGAATCCTTACCATTAAGGATTTAGAGGATATTTCCAAACAACAGGAA
AAAACAAATCTTCCACTCAC

Product: general secretory pathway protein D

Products: NA

Alternate protein names: Pullulanase secretion envelope pulD [H]

Number of amino acids: Translated: 587; Mature: 587

Protein sequence:

>587_residues
MRNRLPSVVLCICLYLIVTPNFSQEKGKPKAKTSQEPASFTADWRDTELKDFLMGMSAIIKKNILIDDAVKGKKITIISQ
KRVRIEDAFGFMKSVLETQGFGLIEENDLIKVVKIKDALAKSQIVRIGKDPVSDSEVALNKTITQIVPLEFSNAIELEPI
LKRVTSPDTDIIIPKNQNTLIFSGSTADINKLLKLVDNLDVRADGPGSISSAGDIHIYTLEYNEAEKLAAILVKLDMPDA
PVAPTTQGAPGEAGADGKPAPPPQPTAQAPKVPGKQDKIKAVAHKESNSLIVTATPQEWEEIKKIIKILDTPRKQVLLEV
LIVELSSTDLNDFGIDWRYQELAYGQFNTGLAAQGGVIDKNGRPTNVNTLSGFSLGFIRRGGQQIIGILNANSTNENFNV
LSAPQILTLDNQEAEINVGQDVPVRTQNRNAGLGGDNAVTVANFEYRPTGIKLKFTPHINKNNRITLDLYQEIKNVAGIS
SEATGGNPTFNKRDIKTTIVVDNIQTIVIGGLLSNDKQKKVQKIPILGEIPLLGTLFRRTTNQNRKTNLMVFLTPHILDD
RDKSDRMTIQKKNEQERMVDEREKKLR

Sequences:

>Translated_587_residues
MRNRLPSVVLCICLYLIVTPNFSQEKGKPKAKTSQEPASFTADWRDTELKDFLMGMSAIIKKNILIDDAVKGKKITIISQ
KRVRIEDAFGFMKSVLETQGFGLIEENDLIKVVKIKDALAKSQIVRIGKDPVSDSEVALNKTITQIVPLEFSNAIELEPI
LKRVTSPDTDIIIPKNQNTLIFSGSTADINKLLKLVDNLDVRADGPGSISSAGDIHIYTLEYNEAEKLAAILVKLDMPDA
PVAPTTQGAPGEAGADGKPAPPPQPTAQAPKVPGKQDKIKAVAHKESNSLIVTATPQEWEEIKKIIKILDTPRKQVLLEV
LIVELSSTDLNDFGIDWRYQELAYGQFNTGLAAQGGVIDKNGRPTNVNTLSGFSLGFIRRGGQQIIGILNANSTNENFNV
LSAPQILTLDNQEAEINVGQDVPVRTQNRNAGLGGDNAVTVANFEYRPTGIKLKFTPHINKNNRITLDLYQEIKNVAGIS
SEATGGNPTFNKRDIKTTIVVDNIQTIVIGGLLSNDKQKKVQKIPILGEIPLLGTLFRRTTNQNRKTNLMVFLTPHILDD
RDKSDRMTIQKKNEQERMVDEREKKLR
>Mature_587_residues
MRNRLPSVVLCICLYLIVTPNFSQEKGKPKAKTSQEPASFTADWRDTELKDFLMGMSAIIKKNILIDDAVKGKKITIISQ
KRVRIEDAFGFMKSVLETQGFGLIEENDLIKVVKIKDALAKSQIVRIGKDPVSDSEVALNKTITQIVPLEFSNAIELEPI
LKRVTSPDTDIIIPKNQNTLIFSGSTADINKLLKLVDNLDVRADGPGSISSAGDIHIYTLEYNEAEKLAAILVKLDMPDA
PVAPTTQGAPGEAGADGKPAPPPQPTAQAPKVPGKQDKIKAVAHKESNSLIVTATPQEWEEIKKIIKILDTPRKQVLLEV
LIVELSSTDLNDFGIDWRYQELAYGQFNTGLAAQGGVIDKNGRPTNVNTLSGFSLGFIRRGGQQIIGILNANSTNENFNV
LSAPQILTLDNQEAEINVGQDVPVRTQNRNAGLGGDNAVTVANFEYRPTGIKLKFTPHINKNNRITLDLYQEIKNVAGIS
SEATGGNPTFNKRDIKTTIVVDNIQTIVIGGLLSNDKQKKVQKIPILGEIPLLGTLFRRTTNQNRKTNLMVFLTPHILDD
RDKSDRMTIQKKNEQERMVDEREKKLR

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of pullulanase [H]

COG id: COG1450

COG function: function code NU; Type II secretory pathway, component PulD

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP D family [H]

Homologues:

Organism=Escherichia coli, GI87082242, Length=471, Percent_Identity=32.2717622080679, Blast_Score=201, Evalue=9e-53,
Organism=Escherichia coli, GI1789793, Length=278, Percent_Identity=24.8201438848921, Blast_Score=94, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001775
- InterPro:   IPR005644
- InterPro:   IPR004846
- InterPro:   IPR013356
- InterPro:   IPR004845 [H]

Pfam domain/function: PF00263 Secretin; PF03958 Secretin_N [H]

EC number: NA

Molecular weight: Translated: 64717; Mature: 64717

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNRLPSVVLCICLYLIVTPNFSQEKGKPKAKTSQEPASFTADWRDTELKDFLMGMSAII
CCCCCHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
KKNILIDDAVKGKKITIISQKRVRIEDAFGFMKSVLETQGFGLIEENDLIKVVKIKDALA
HCCCEEECCCCCCEEEEEECCCEEHHHHHHHHHHHHHHCCCCEEECCCCEEEEEHHHHHH
KSQIVRIGKDPVSDSEVALNKTITQIVPLEFSNAIELEPILKRVTSPDTDIIIPKNQNTL
HHHHHCCCCCCCCCCHHHHHHHHHHEECCCCCCCEEHHHHHHHHCCCCCCEEEECCCCEE
IFSGSTADINKLLKLVDNLDVRADGPGSISSAGDIHIYTLEYNEAEKLAAILVKLDMPDA
EEECCCHHHHHHHHHHHCCCEECCCCCCCCCCCCEEEEEEECCCHHHEEEEEEEECCCCC
PVAPTTQGAPGEAGADGKPAPPPQPTAQAPKVPGKQDKIKAVAHKESNSLIVTATPQEWE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHEEEECCCCCEEEEECCHHHH
EIKKIIKILDTPRKQVLLEVLIVELSSTDLNDFGIDWRYQELAYGQFNTGLAAQGGVIDK
HHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEHHEECCCCCCCCCCCCCEECC
NGRPTNVNTLSGFSLGFIRRGGQQIIGILNANSTNENFNVLSAPQILTLDNQEAEINVGQ
CCCCCCCCCCCCCEEHHHHCCCCCEEEEEECCCCCCCCEEEECCEEEEECCCCCEEECCC
DVPVRTQNRNAGLGGDNAVTVANFEYRPTGIKLKFTPHINKNNRITLDLYQEIKNVAGIS
CCCCEECCCCCCCCCCCEEEEEEEEECCCCEEEEEECCCCCCCEEEEHHHHHHHHHCCCC
SEATGGNPTFNKRDIKTTIVVDNIQTIVIGGLLSNDKQKKVQKIPILGEIPLLGTLFRRT
CCCCCCCCCCCCCCEEEEEEEECCEEEEEEHHHCCCHHHHHHHCCCEECCCHHHHHHHHH
TNQNRKTNLMVFLTPHILDDRDKSDRMTIQKKNEQERMVDEREKKLR
CCCCCCEEEEEEECCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MRNRLPSVVLCICLYLIVTPNFSQEKGKPKAKTSQEPASFTADWRDTELKDFLMGMSAII
CCCCCHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
KKNILIDDAVKGKKITIISQKRVRIEDAFGFMKSVLETQGFGLIEENDLIKVVKIKDALA
HCCCEEECCCCCCEEEEEECCCEEHHHHHHHHHHHHHHCCCCEEECCCCEEEEEHHHHHH
KSQIVRIGKDPVSDSEVALNKTITQIVPLEFSNAIELEPILKRVTSPDTDIIIPKNQNTL
HHHHHCCCCCCCCCCHHHHHHHHHHEECCCCCCCEEHHHHHHHHCCCCCCEEEECCCCEE
IFSGSTADINKLLKLVDNLDVRADGPGSISSAGDIHIYTLEYNEAEKLAAILVKLDMPDA
EEECCCHHHHHHHHHHHCCCEECCCCCCCCCCCCEEEEEEECCCHHHEEEEEEEECCCCC
PVAPTTQGAPGEAGADGKPAPPPQPTAQAPKVPGKQDKIKAVAHKESNSLIVTATPQEWE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHEEEECCCCCEEEEECCHHHH
EIKKIIKILDTPRKQVLLEVLIVELSSTDLNDFGIDWRYQELAYGQFNTGLAAQGGVIDK
HHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEHHEECCCCCCCCCCCCCEECC
NGRPTNVNTLSGFSLGFIRRGGQQIIGILNANSTNENFNVLSAPQILTLDNQEAEINVGQ
CCCCCCCCCCCCCEEHHHHCCCCCEEEEEECCCCCCCCEEEECCEEEEECCCCCEEECCC
DVPVRTQNRNAGLGGDNAVTVANFEYRPTGIKLKFTPHINKNNRITLDLYQEIKNVAGIS
CCCCEECCCCCCCCCCCEEEEEEEEECCCCEEEEEECCCCCCCEEEEHHHHHHHHHCCCC
SEATGGNPTFNKRDIKTTIVVDNIQTIVIGGLLSNDKQKKVQKIPILGEIPLLGTLFRRT
CCCCCCCCCCCCCCEEEEEEEECCEEEEEEHHHCCCHHHHHHHCCCEECCCHHHHHHHHH
TNQNRKTNLMVFLTPHILDDRDKSDRMTIQKKNEQERMVDEREKKLR
CCCCCCEEEEEEECCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2677007 [H]