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The map label for this gene is trmD [H]

Identifier: 183221050

GI number: 183221050

Start: 1737393

End: 1738046

Strand: Direct

Name: trmD [H]

Synonym: LEPBI_I1663

Alternate gene names: 183221050

Gene position: 1737393-1738046 (Clockwise)

Preceding gene: 183221049

Following gene: 183221051

Centisome position: 48.27

GC content: 46.02

Gene sequence:

>654_bases
TTGCGCTTTAATTTCATCACACTATTCCCCGAAAAAATCACTTCTTATTTTGATACTGGGATCCCTGGAAAAGCAGTGAA
ACAAGGGGTCGTCGAAATCAATCCTGTACACTTACGAGACTTCGCCGACAACAAACACCAAAAGGTAGATGATACGATTT
ACGGGGGAGGTCCTGGCATGCTCTTGCAAGTGGGGCCAATTTACCGTGCCCTGGAATCCTTGGGAGACAAAAAAGGAAAG
GTGATTTTACTCAGTCCCTCGGGGGAACTGTTCAACCAAACACTCGCTCGTGAAATTTACGAGTCCTCGGACACCTTCAC
CTTGGTTTCCGGGTATTATGAAGGGGTCGACCACCGTGTCACGGAGCATTTAATTGACAGGGAAGTGGCCATTGGAAACT
ATGTTATTTCATCGGGGGATTTAGCTGCTCTCGTTGTTGCCGATTGCCTGTCTCGGTTTGTTCCGGGGTTTTTGGGTAAG
GAAGAAAGCCTTCTAGAAGAATCGCACAACGAAACGGAAGAATTAGAATACCCCCAGTATACAAAACCCTATGATTTTAT
GGGTTGGACTGTTCCAGATGTACTCCTCGGTGGACATCATGAAGAGATCCGGAAATGGCGGCAAAAAAACCGCAAAACAA
GAAATCATTCTTAG

Upstream 100 bases:

>100_bases
GGGGAAGGAGAGACAATACTCGTTCCTTTCCTAAACCAATTTGTGGGTGATTGGGATTTAGAAAACCAAACCATCGAATG
CATTCATTGGGAGCAGTGGT

Downstream 100 bases:

>100_bases
AGGAAGCCATGAATCAGATTCTAGAAACAGCACTCGCAGGCGAAGCAAAGAACGAACTTAATTTCGAAATTGGTGATACA
GTCAAAGTTCACTACAAAAT

Product: fused tRNA (guanine-N(1)-)-methyltransferase/unknown domain protein

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MRFNFITLFPEKITSYFDTGIPGKAVKQGVVEINPVHLRDFADNKHQKVDDTIYGGGPGMLLQVGPIYRALESLGDKKGK
VILLSPSGELFNQTLAREIYESSDTFTLVSGYYEGVDHRVTEHLIDREVAIGNYVISSGDLAALVVADCLSRFVPGFLGK
EESLLEESHNETEELEYPQYTKPYDFMGWTVPDVLLGGHHEEIRKWRQKNRKTRNHS

Sequences:

>Translated_217_residues
MRFNFITLFPEKITSYFDTGIPGKAVKQGVVEINPVHLRDFADNKHQKVDDTIYGGGPGMLLQVGPIYRALESLGDKKGK
VILLSPSGELFNQTLAREIYESSDTFTLVSGYYEGVDHRVTEHLIDREVAIGNYVISSGDLAALVVADCLSRFVPGFLGK
EESLLEESHNETEELEYPQYTKPYDFMGWTVPDVLLGGHHEEIRKWRQKNRKTRNHS
>Mature_217_residues
MRFNFITLFPEKITSYFDTGIPGKAVKQGVVEINPVHLRDFADNKHQKVDDTIYGGGPGMLLQVGPIYRALESLGDKKGK
VILLSPSGELFNQTLAREIYESSDTFTLVSGYYEGVDHRVTEHLIDREVAIGNYVISSGDLAALVVADCLSRFVPGFLGK
EESLLEESHNETEELEYPQYTKPYDFMGWTVPDVLLGGHHEEIRKWRQKNRKTRNHS

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=218, Percent_Identity=43.1192660550459, Blast_Score=165, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 24549; Mature: 24549

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00211 ABC_TRANSPORTER_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFNFITLFPEKITSYFDTGIPGKAVKQGVVEINPVHLRDFADNKHQKVDDTIYGGGPGM
CCCEEEEECHHHHHHHHCCCCCHHHHHCCCEECCCEEEHHCCCCCCCCCCCCEECCCCCE
LLQVGPIYRALESLGDKKGKVILLSPSGELFNQTLAREIYESSDTFTLVSGYYEGVDHRV
EEEECHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCEEEEHHHHHCHHHHH
TEHLIDREVAIGNYVISSGDLAALVVADCLSRFVPGFLGKEESLLEESHNETEELEYPQY
HHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHCCCCCC
TKPYDFMGWTVPDVLLGGHHEEIRKWRQKNRKTRNHS
CCCCCCCCCCHHHHHHCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRFNFITLFPEKITSYFDTGIPGKAVKQGVVEINPVHLRDFADNKHQKVDDTIYGGGPGM
CCCEEEEECHHHHHHHHCCCCCHHHHHCCCEECCCEEEHHCCCCCCCCCCCCEECCCCCE
LLQVGPIYRALESLGDKKGKVILLSPSGELFNQTLAREIYESSDTFTLVSGYYEGVDHRV
EEEECHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCEEEEHHHHHCHHHHH
TEHLIDREVAIGNYVISSGDLAALVVADCLSRFVPGFLGKEESLLEESHNETEELEYPQY
HHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHCCCCCC
TKPYDFMGWTVPDVLLGGHHEEIRKWRQKNRKTRNHS
CCCCCCCCCCHHHHHHCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA