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The map label for this gene is purH

Identifier: 183220856

GI number: 183220856

Start: 1529869

End: 1531410

Strand: Reverse

Name: purH

Synonym: LEPBI_I1469

Alternate gene names: 183220856

Gene position: 1531410-1529869 (Counterclockwise)

Preceding gene: 183220857

Following gene: 183220855

Centisome position: 42.54

GC content: 41.18

Gene sequence:

>1542_bases
ATGATTCAAATCAAAAGAGCACTTGTTTCCGTTTCTGATAAAACGGGAATCACAGAAATCTGTTCCTTCCTAACCAAACA
CGGCGTGGAAATTTTATCCACCGGTGGAACATACGATGCCCTTTCCAAAGCAGGGATCGCTGTGAAAAAGGTAGATGAGT
TCACTGGTTTCCCAGAAATTTTACATGGTCGAGTGAAAACCCTTCACCCTAAAATCCATGGAGGGTTACTAGGAGACACA
ACGAACCCCGATCACGTCAAACAAATGGAATCCAATGGGATTGTTCCCATCACACTTGTCATTGTGAATTTATATCCTTT
TGTCAAAACTGTGATGAAACCAGATGTCACTTTAGAAGATGCGATTGAAAACATTGATATTGGTGGACCGTCGATGCTCC
GTTCGGCGGCAAAAAATCACAAAAACGTTGTGGTTCTCACCGATCCAAAGGATTATGAATCCTTTCAAAACGAATTCACG
ACAAACAATGGAAAGATTTCAAGAGAAACTGCTTTCGGTTATGCCGCAAAAGTATTTTCAGAAACCGCATCCTATGATTC
CGCCATTTCTTCCTACTTTAACAAACGTTTAGGGATCAAATATCCTGATAAAATCACTTTTGCCTTTAATAAAAAACAAA
AATTACGCTATGGGGAGAACCCACACCAAGATGCTGCATTTTACGAACCACTCTTTCTCAAATCGCAATTCGAAGCATTA
CAAGGGAAAGAACTTTCATTTAATAATATGTTGGATTTTGATGCTGCATTTCACGTAGCAAGTTTACTTCCGAAAAATGC
AGTCTCGATCGTGAAACATTTAAATCCTTGTGGGATTGCCTTTGGTGAAACTGTTTTAGAATCGTTTGAACTAGCGAGAA
AAACCGATCCTATTTCTGCCTTTGGTGGAATCATCGGAATCCATGGGCGAGTGGAAAAAGAATCTGCGGAAGAGATTACA
AAAAACTTCGTGGAAGGTGTGATTGCCGAAAGTTTTTCCAATGAAGCCTTAGAAATTTTTGCAAAGAAACCAAACATCCG
TTTGATCCCGATCGCAAAATTTGATGAAGCACTCGATGAACTTGACTTACGATCCCTCCACCACGGGCTTCTCATTCAAA
ATAGAGATTATGATTTGATCACAAAAGACAAACTAAAAATTGTTTCGAAAAAACAACCTACCGAAGATGATTTGGAAGGT
TTGATGTTTGCTTGGAATTGTGTGAAATTCATCAAGTCCAATGCAATTGTGTATACAGACCAAAACTCAACGCTTGGAAT
TGGTGCAGGCCAAATGTCTCGTGTGGATTCCGTAGAACTCGGTGCCATGAAAGCTCAGAAAGTAGGACTCTCCGTTGTGG
GTTCTTATGTAGGGAGTGATGCCTTTTTCCCATTTCGGGATGGGATTGATGCCATTGCCAAGGTTGGTGCCAAAGCGATC
ATCCAACCAGGCGGATCCATCCGTGACGAGGAAGTGATCCAAGCCGCTGATGAACATGGGCTCATTATGGTCTTCACTGG
TATGAGGCATTTCCGTCACTAA

Upstream 100 bases:

>100_bases
TGGCAATACTAAAGGAAGAACATATAATCCTTCCACTCGCCATACAACTGTTTTGTGAAGATAAATTAAAAATCAAAGAA
CGAAAGGTAGAAATCCTAAA

Downstream 100 bases:

>100_bases
TGGAATTTTTCCTTTTCCTACTCTTTTTATTCGTTTTTGGATTTGTAGCAGCAGCGATATCATATTACGCGAAGATATTG
TTTTTTTCTGCGAAATCAAA

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase

Number of amino acids: Translated: 513; Mature: 513

Protein sequence:

>513_residues
MIQIKRALVSVSDKTGITEICSFLTKHGVEILSTGGTYDALSKAGIAVKKVDEFTGFPEILHGRVKTLHPKIHGGLLGDT
TNPDHVKQMESNGIVPITLVIVNLYPFVKTVMKPDVTLEDAIENIDIGGPSMLRSAAKNHKNVVVLTDPKDYESFQNEFT
TNNGKISRETAFGYAAKVFSETASYDSAISSYFNKRLGIKYPDKITFAFNKKQKLRYGENPHQDAAFYEPLFLKSQFEAL
QGKELSFNNMLDFDAAFHVASLLPKNAVSIVKHLNPCGIAFGETVLESFELARKTDPISAFGGIIGIHGRVEKESAEEIT
KNFVEGVIAESFSNEALEIFAKKPNIRLIPIAKFDEALDELDLRSLHHGLLIQNRDYDLITKDKLKIVSKKQPTEDDLEG
LMFAWNCVKFIKSNAIVYTDQNSTLGIGAGQMSRVDSVELGAMKAQKVGLSVVGSYVGSDAFFPFRDGIDAIAKVGAKAI
IQPGGSIRDEEVIQAADEHGLIMVFTGMRHFRH

Sequences:

>Translated_513_residues
MIQIKRALVSVSDKTGITEICSFLTKHGVEILSTGGTYDALSKAGIAVKKVDEFTGFPEILHGRVKTLHPKIHGGLLGDT
TNPDHVKQMESNGIVPITLVIVNLYPFVKTVMKPDVTLEDAIENIDIGGPSMLRSAAKNHKNVVVLTDPKDYESFQNEFT
TNNGKISRETAFGYAAKVFSETASYDSAISSYFNKRLGIKYPDKITFAFNKKQKLRYGENPHQDAAFYEPLFLKSQFEAL
QGKELSFNNMLDFDAAFHVASLLPKNAVSIVKHLNPCGIAFGETVLESFELARKTDPISAFGGIIGIHGRVEKESAEEIT
KNFVEGVIAESFSNEALEIFAKKPNIRLIPIAKFDEALDELDLRSLHHGLLIQNRDYDLITKDKLKIVSKKQPTEDDLEG
LMFAWNCVKFIKSNAIVYTDQNSTLGIGAGQMSRVDSVELGAMKAQKVGLSVVGSYVGSDAFFPFRDGIDAIAKVGAKAI
IQPGGSIRDEEVIQAADEHGLIMVFTGMRHFRH
>Mature_513_residues
MIQIKRALVSVSDKTGITEICSFLTKHGVEILSTGGTYDALSKAGIAVKKVDEFTGFPEILHGRVKTLHPKIHGGLLGDT
TNPDHVKQMESNGIVPITLVIVNLYPFVKTVMKPDVTLEDAIENIDIGGPSMLRSAAKNHKNVVVLTDPKDYESFQNEFT
TNNGKISRETAFGYAAKVFSETASYDSAISSYFNKRLGIKYPDKITFAFNKKQKLRYGENPHQDAAFYEPLFLKSQFEAL
QGKELSFNNMLDFDAAFHVASLLPKNAVSIVKHLNPCGIAFGETVLESFELARKTDPISAFGGIIGIHGRVEKESAEEIT
KNFVEGVIAESFSNEALEIFAKKPNIRLIPIAKFDEALDELDLRSLHHGLLIQNRDYDLITKDKLKIVSKKQPTEDDLEG
LMFAWNCVKFIKSNAIVYTDQNSTLGIGAGQMSRVDSVELGAMKAQKVGLSVVGSYVGSDAFFPFRDGIDAIAKVGAKAI
IQPGGSIRDEEVIQAADEHGLIMVFTGMRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family

Homologues:

Organism=Homo sapiens, GI20127454, Length=469, Percent_Identity=37.1002132196162, Blast_Score=255, Evalue=6e-68,
Organism=Escherichia coli, GI1790439, Length=525, Percent_Identity=49.3333333333333, Blast_Score=520, Evalue=1e-148,
Organism=Caenorhabditis elegans, GI71985564, Length=606, Percent_Identity=30.3630363036304, Blast_Score=253, Evalue=1e-67,
Organism=Caenorhabditis elegans, GI71985574, Length=345, Percent_Identity=25.2173913043478, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI71985556, Length=92, Percent_Identity=39.1304347826087, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6323768, Length=475, Percent_Identity=34.9473684210526, Blast_Score=233, Evalue=6e-62,
Organism=Saccharomyces cerevisiae, GI6323056, Length=480, Percent_Identity=34.7916666666667, Blast_Score=229, Evalue=7e-61,
Organism=Drosophila melanogaster, GI24649832, Length=482, Percent_Identity=35.0622406639004, Blast_Score=253, Evalue=2e-67,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PUR9_LEPBA (B0SGW3)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001962508.1
- ProteinModelPortal:   B0SGW3
- SMR:   B0SGW3
- GeneID:   6387421
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_1416
- HOGENOM:   HBG498048
- OMA:   ASDGFFP
- ProtClustDB:   PRK00881
- BioCyc:   LBIF355278:LBF_1416-MONOMER
- HAMAP:   MF_00139
- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607
- Gene3D:   G3DSA:3.40.50.1380
- PANTHER:   PTHR11692
- PIRSF:   PIRSF000414
- SMART:   SM00798
- SMART:   SM00851
- TIGRFAMs:   TIGR00355

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS; SSF53927 Cytidine_deaminase-like; SSF52335 MGS-like_dom

EC number: =2.1.2.3; =3.5.4.10

Molecular weight: Translated: 56495; Mature: 56495

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQIKRALVSVSDKTGITEICSFLTKHGVEILSTGGTYDALSKAGIAVKKVDEFTGFPEI
CCEEHHHHHHCCCCCCHHHHHHHHHHCCCEEEECCCCHHHHHHCCCHHHHHHHHCCCHHH
LHGRVKTLHPKIHGGLLGDTTNPDHVKQMESNGIVPITLVIVNLYPFVKTVMKPDVTLED
HHCHHHHCCCHHCCCCCCCCCCHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCCCHHH
AIENIDIGGPSMLRSAAKNHKNVVVLTDPKDYESFQNEFTTNNGKISRETAFGYAAKVFS
HHHHCCCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHH
ETASYDSAISSYFNKRLGIKYPDKITFAFNKKQKLRYGENPHQDAAFYEPLFLKSQFEAL
HHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHCCCCCCCCCCHHHCHHHHHHHHHHH
QGKELSFNNMLDFDAAFHVASLLPKNAVSIVKHLNPCGIAFGETVLESFELARKTDPISA
CCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHH
FGGIIGIHGRVEKESAEEITKNFVEGVIAESFSNEALEIFAKKPNIRLIPIAKFDEALDE
HCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEECHHHHHHHHH
LDLRSLHHGLLIQNRDYDLITKDKLKIVSKKQPTEDDLEGLMFAWNCVKFIKSNAIVYTD
HHHHHHHCCCEEECCCCCCEEHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEC
QNSTLGIGAGQMSRVDSVELGAMKAQKVGLSVVGSYVGSDAFFPFRDGIDAIAKVGAKAI
CCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCHHHHHHCCCCEE
IQPGGSIRDEEVIQAADEHGLIMVFTGMRHFRH
ECCCCCCCHHHHHHHHCCCCEEEEEECHHHHCC
>Mature Secondary Structure
MIQIKRALVSVSDKTGITEICSFLTKHGVEILSTGGTYDALSKAGIAVKKVDEFTGFPEI
CCEEHHHHHHCCCCCCHHHHHHHHHHCCCEEEECCCCHHHHHHCCCHHHHHHHHCCCHHH
LHGRVKTLHPKIHGGLLGDTTNPDHVKQMESNGIVPITLVIVNLYPFVKTVMKPDVTLED
HHCHHHHCCCHHCCCCCCCCCCHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCCCHHH
AIENIDIGGPSMLRSAAKNHKNVVVLTDPKDYESFQNEFTTNNGKISRETAFGYAAKVFS
HHHHCCCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHH
ETASYDSAISSYFNKRLGIKYPDKITFAFNKKQKLRYGENPHQDAAFYEPLFLKSQFEAL
HHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHCCCCCCCCCCHHHCHHHHHHHHHHH
QGKELSFNNMLDFDAAFHVASLLPKNAVSIVKHLNPCGIAFGETVLESFELARKTDPISA
CCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHH
FGGIIGIHGRVEKESAEEITKNFVEGVIAESFSNEALEIFAKKPNIRLIPIAKFDEALDE
HCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEECHHHHHHHHH
LDLRSLHHGLLIQNRDYDLITKDKLKIVSKKQPTEDDLEGLMFAWNCVKFIKSNAIVYTD
HHHHHHHCCCEEECCCCCCEEHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEC
QNSTLGIGAGQMSRVDSVELGAMKAQKVGLSVVGSYVGSDAFFPFRDGIDAIAKVGAKAI
CCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCHHHHHHCCCCEE
IQPGGSIRDEEVIQAADEHGLIMVFTGMRHFRH
ECCCCCCCHHHHHHHHCCCCEEEEEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA