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The map label for this gene is purN [H]

Identifier: 183220857

GI number: 183220857

Start: 1531407

End: 1532021

Strand: Reverse

Name: purN [H]

Synonym: LEPBI_I1470

Alternate gene names: 183220857

Gene position: 1532021-1531407 (Counterclockwise)

Preceding gene: 183220861

Following gene: 183220856

Centisome position: 42.56

GC content: 42.93

Gene sequence:

>615_bases
ATGGGAAAAACAAAACGTGTTGTTTTTTTAGCTTCGGGCCGAGGGTCCAATTTTTCGGCGGCGGTGGAATCCATCCAAAA
AAAGAAGCTAAAATTGGACATCCTTGCCCTCGTTTCCGACAATCCGGAAGCAAAGGCTCTTACCATTGCCAAAAACTTCG
GGATTTCCACCAAAGTGATCCCATACGGCTCTTACCAATCCAAATCCGACTACCACAGGGATTTACTCAGGCAAGTTGAG
GCGTATGACCCTGACTTAATCGTAGCATGCGGTTACATGCGAATTCTGAAACCAGAATTTGTCCAAAGGTTCAAAAACCA
AATCATCAATGTCCACCCGAGCCTGCTCCCAGCGTTTCCAGGACTTGATTCTCAAAAACAAGCCTTGGATTATGGAGTCA
AAGTGGCAGGATGCACTGTCCATTTTGTTTGGGAAGGTGTGGATACAGGACCCATCATTTTACAAAAAGCGATTGCCATT
CGACCGGAATGGACTGAAAAAGAATTATCCTTGGCAATACTAAAGGAAGAACATATAATCCTTCCACTCGCCATACAACT
GTTTTGTGAAGATAAATTAAAAATCAAAGAACGAAAGGTAGAAATCCTAAAATGA

Upstream 100 bases:

>100_bases
CCGGTTTTTCTCGCAAGCGACATTTTTTTCTCCTGTGTATTCCCATCGACCAAAAAGGATACTTGCTTAATGACCGATCC
GAATATTCTTAGATTATCAC

Downstream 100 bases:

>100_bases
TTCAAATCAAAAGAGCACTTGTTTCCGTTTCTGATAAAACGGGAATCACAGAAATCTGTTCCTTCCTAACCAAACACGGC
GTGGAAATTTTATCCACCGG

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 204; Mature: 203

Protein sequence:

>204_residues
MGKTKRVVFLASGRGSNFSAAVESIQKKKLKLDILALVSDNPEAKALTIAKNFGISTKVIPYGSYQSKSDYHRDLLRQVE
AYDPDLIVACGYMRILKPEFVQRFKNQIINVHPSLLPAFPGLDSQKQALDYGVKVAGCTVHFVWEGVDTGPIILQKAIAI
RPEWTEKELSLAILKEEHIILPLAIQLFCEDKLKIKERKVEILK

Sequences:

>Translated_204_residues
MGKTKRVVFLASGRGSNFSAAVESIQKKKLKLDILALVSDNPEAKALTIAKNFGISTKVIPYGSYQSKSDYHRDLLRQVE
AYDPDLIVACGYMRILKPEFVQRFKNQIINVHPSLLPAFPGLDSQKQALDYGVKVAGCTVHFVWEGVDTGPIILQKAIAI
RPEWTEKELSLAILKEEHIILPLAIQLFCEDKLKIKERKVEILK
>Mature_203_residues
GKTKRVVFLASGRGSNFSAAVESIQKKKLKLDILALVSDNPEAKALTIAKNFGISTKVIPYGSYQSKSDYHRDLLRQVEA
YDPDLIVACGYMRILKPEFVQRFKNQIINVHPSLLPAFPGLDSQKQALDYGVKVAGCTVHFVWEGVDTGPIILQKAIAIR
PEWTEKELSLAILKEEHIILPLAIQLFCEDKLKIKERKVEILK

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=196, Percent_Identity=37.2448979591837, Blast_Score=139, Evalue=1e-33,
Organism=Homo sapiens, GI209869995, Length=196, Percent_Identity=37.2448979591837, Blast_Score=139, Evalue=1e-33,
Organism=Homo sapiens, GI209869993, Length=196, Percent_Identity=37.2448979591837, Blast_Score=139, Evalue=1e-33,
Organism=Escherichia coli, GI1788846, Length=193, Percent_Identity=36.2694300518135, Blast_Score=155, Evalue=3e-39,
Organism=Escherichia coli, GI1787483, Length=168, Percent_Identity=31.547619047619, Blast_Score=105, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17567511, Length=183, Percent_Identity=38.2513661202186, Blast_Score=133, Evalue=6e-32,
Organism=Saccharomyces cerevisiae, GI6320616, Length=202, Percent_Identity=29.7029702970297, Blast_Score=78, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24582400, Length=188, Percent_Identity=40.4255319148936, Blast_Score=145, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 22917; Mature: 22786

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKTKRVVFLASGRGSNFSAAVESIQKKKLKLDILALVSDNPEAKALTIAKNFGISTKVI
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHEEEEEEEECCCCCCEEEEEEECCCCCEEEE
PYGSYQSKSDYHRDLLRQVEAYDPDLIVACGYMRILKPEFVQRFKNQIINVHPSLLPAFP
ECCCCCCCCHHHHHHHHHHHHCCCCEEEECCHHHHCCHHHHHHHHHHHEECCCCHHCCCC
GLDSQKQALDYGVKVAGCTVHFVWEGVDTGPIILQKAIAIRPEWTEKELSLAILKEEHII
CCCCHHHHHHCCEEEECEEEEEEEECCCCCHHHHHHHHHCCCCCCCHHHEEEEECCCCEE
LPLAIQLFCEDKLKIKERKVEILK
EHHHHEEHHCCCHHHHHHHHEECC
>Mature Secondary Structure 
GKTKRVVFLASGRGSNFSAAVESIQKKKLKLDILALVSDNPEAKALTIAKNFGISTKVI
CCCEEEEEEECCCCCCHHHHHHHHHHHHHEEEEEEEECCCCCCEEEEEEECCCCCEEEE
PYGSYQSKSDYHRDLLRQVEAYDPDLIVACGYMRILKPEFVQRFKNQIINVHPSLLPAFP
ECCCCCCCCHHHHHHHHHHHHCCCCEEEECCHHHHCCHHHHHHHHHHHEECCCCHHCCCC
GLDSQKQALDYGVKVAGCTVHFVWEGVDTGPIILQKAIAIRPEWTEKELSLAILKEEHII
CCCCHHHHHHCCEEEECEEEEEEEECCCCCHHHHHHHHHCCCCCCCHHHEEEEECCCCEE
LPLAIQLFCEDKLKIKERKVEILK
EHHHHEEHHCCCHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]