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The map label for this gene is lexA

Identifier: 183220706

GI number: 183220706

Start: 1372939

End: 1373547

Strand: Direct

Name: lexA

Synonym: LEPBI_I1316

Alternate gene names: 183220706

Gene position: 1372939-1373547 (Clockwise)

Preceding gene: 183220705

Following gene: 183220707

Centisome position: 38.14

GC content: 47.45

Gene sequence:

>609_bases
ATGAAAGACCTCACGGAAAAACAAGAATTTGTTTTACAATACATCTCGGACACAGTACGAGAAAAGGGATTTCCACCCAC
GATCCGTGAGATTGGAGACCAATTTGGGATCACTGCCAAAGGGGCCTATGACCACCTCAAGGCCATCGAAAAAAAAGGAT
ACATCCGCACCTCTAAAAACCAGAGCCGTGCCATCGAACTCCTGAAAGGGAATGCCGATGAGGCACTCCTGGTTCGTGCT
TCCGGGATTCCTCTCCTCGGACAGGTGGCAGCGGGAGCTCCCATCTTGGCAGAAGAAAATATTGAAGAATACATCGCCGT
TCCTGATGATCTGGCAACAAAACCGGGAACCTTTGCCCTCCGCGTCAAAGGGGATTCCATGGTGGAAGCCGGGATCAGTG
ATGGAGACATTGCCATCATCCAAAAGAAAGATACTGCTCGTAATGGTGAAATTGTAGTGGCGCTGATTGAAAATGAAGCC
ACCCTCAAGGTTTTTTTTAAGGAACCAGACATGATCCGTTTGGAACCAAGAAATGCAAAACTCAAACCCATTCGCACAAA
GAAAGCGACGATTATCGGAAAACTGATCGGTCTCTATCGCATTTACTGA

Upstream 100 bases:

>100_bases
TTGTGGAAGAAGGAAGTTTTGTCTTAAAAAGTGAATATTTAAAACTATAACAACTAGTTTTTTGTTGACATAACGCCCAT
TTCCTATTTATCTGTTTAGT

Downstream 100 bases:

>100_bases
TTGACCAAGAGTAGGTTTCCTTCGATCTTAGAAGGGATGTCGAAACATCTCTTCCTTTCAGTCTGTCTCGGATTCACTAT
TTTCCTCGTTAGTTGTGCTC

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 202; Mature: 202

Protein sequence:

>202_residues
MKDLTEKQEFVLQYISDTVREKGFPPTIREIGDQFGITAKGAYDHLKAIEKKGYIRTSKNQSRAIELLKGNADEALLVRA
SGIPLLGQVAAGAPILAEENIEEYIAVPDDLATKPGTFALRVKGDSMVEAGISDGDIAIIQKKDTARNGEIVVALIENEA
TLKVFFKEPDMIRLEPRNAKLKPIRTKKATIIGKLIGLYRIY

Sequences:

>Translated_202_residues
MKDLTEKQEFVLQYISDTVREKGFPPTIREIGDQFGITAKGAYDHLKAIEKKGYIRTSKNQSRAIELLKGNADEALLVRA
SGIPLLGQVAAGAPILAEENIEEYIAVPDDLATKPGTFALRVKGDSMVEAGISDGDIAIIQKKDTARNGEIVVALIENEA
TLKVFFKEPDMIRLEPRNAKLKPIRTKKATIIGKLIGLYRIY
>Mature_202_residues
MKDLTEKQEFVLQYISDTVREKGFPPTIREIGDQFGITAKGAYDHLKAIEKKGYIRTSKNQSRAIELLKGNADEALLVRA
SGIPLLGQVAAGAPILAEENIEEYIAVPDDLATKPGTFALRVKGDSMVEAGISDGDIAIIQKKDTARNGEIVVALIENEA
TLKVFFKEPDMIRLEPRNAKLKPIRTKKATIIGKLIGLYRIY

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family

Homologues:

Organism=Escherichia coli, GI1790476, Length=205, Percent_Identity=38.5365853658537, Blast_Score=131, Evalue=3e-32,
Organism=Escherichia coli, GI1787431, Length=103, Percent_Identity=35.9223300970874, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEXA_LEPBA (B0SFV4)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001962362.1
- ProteinModelPortal:   B0SFV4
- SMR:   B0SFV4
- MEROPS:   S24.001
- GeneID:   6387522
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_1262
- HOGENOM:   HBG679610
- OMA:   IAENPDF
- ProtClustDB:   PRK00215
- BioCyc:   LBIF355278:LBF_1262-MONOMER
- GO:   GO:0006508
- GO:   GO:0006350
- HAMAP:   MF_00015
- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991
- Gene3D:   G3DSA:2.10.109.10
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00726
- TIGRFAMs:   TIGR00498

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.88

Molecular weight: Translated: 22267; Mature: 22267

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: NA

Important sites: ACT_SITE 126-126 ACT_SITE 163-163

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDLTEKQEFVLQYISDTVREKGFPPTIREIGDQFGITAKGAYDHLKAIEKKGYIRTSKN
CCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCEECCHHHHHHHHHHCCCEEECCC
QSRAIELLKGNADEALLVRASGIPLLGQVAAGAPILAEENIEEYIAVPDDLATKPGTFAL
HHHHHHEECCCCCCEEEEEECCCCEEHHHHCCCCEEECCCCHHHCCCCHHHCCCCCCEEE
RVKGDSMVEAGISDGDIAIIQKKDTARNGEIVVALIENEATLKVFFKEPDMIRLEPRNAK
EECCCCEEECCCCCCCEEEEEECCCCCCCCEEEEEECCCCEEEEEECCCCEEEECCCCCC
LKPIRTKKATIIGKLIGLYRIY
CCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKDLTEKQEFVLQYISDTVREKGFPPTIREIGDQFGITAKGAYDHLKAIEKKGYIRTSKN
CCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCEECCHHHHHHHHHHCCCEEECCC
QSRAIELLKGNADEALLVRASGIPLLGQVAAGAPILAEENIEEYIAVPDDLATKPGTFAL
HHHHHHEECCCCCCEEEEEECCCCEEHHHHCCCCEEECCCCHHHCCCCHHHCCCCCCEEE
RVKGDSMVEAGISDGDIAIIQKKDTARNGEIVVALIENEATLKVFFKEPDMIRLEPRNAK
EECCCCEEECCCCCCCEEEEEECCCCCCCCEEEEEECCCCEEEEEECCCCEEEECCCCCC
LKPIRTKKATIIGKLIGLYRIY
CCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA