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The map label for this gene is yfhQ [H]

Identifier: 183220677

GI number: 183220677

Start: 1342450

End: 1343511

Strand: Direct

Name: yfhQ [H]

Synonym: LEPBI_I1287

Alternate gene names: 183220677

Gene position: 1342450-1343511 (Clockwise)

Preceding gene: 183220676

Following gene: 183220678

Centisome position: 37.29

GC content: 38.98

Gene sequence:

>1062_bases
TTGAACCCACAAAAGAAACTCCGCGATTGGTATCTTTTACACAAACGGGATTTACCTTTTCGTAAAAAGAAACAAGCTTA
TCCCATTTGGATTTCGGAAGTGATGTTGCAACAAACGAGGGTAGCCGCGATGTTACCTCTCTTTGAAAATTTTGTAAACC
GATTCCCAAACCCAGAAAGCCTTGCAAAAGCGACTGAAGAAGAAGTCCTTTCTTTTTGGAAAGGACTAGGGTATTATAGT
CGTGCTCGTAATATTAGAAAGGCTGCAATCCAAATTGTCCAACAATACAACGGCTCTTTTCCCAAAGACTTAGATTCTGT
ATTAAAACTTCCAGGCATTGGTAACTATACAGCACGTGCGATTTTATCAATCTCCTATGATTTACCTTTGGCAGTTCTAG
ATGGAAACGTAAAACGTGTTTTGTCACGTTATTATGGTTATACGAAAAATATTCTCGGACCTCAAGCGGAGAAAGAATTA
CAACTGAAGGCAGATGGGTTTTTGAACCTAGACTTTCCTGGAGACCATAACCAAGCGGTGATGGAACTTGGAGCTACCAT
CTGTTTGCCAGAGTCTCCCAAATGTTTGGTTTGTCCTTTGATGGATGGCTGTTATGCAAGGATCCATGGAAAAACAAAAG
AGATCCCCCTTCGAGAAAAAAAACAAAAACAAGTGCTCCTAACTGGTGAGATTTGGGTCATCCAACAAAAAAATTTGATC
CTTCTCATCAAAGAAAAAAAGAACCGGTTTTTAAAAGGGATGTTCCATCTCCCCTCTGGTTTTTTAGGTGAAATTCCAAA
CAGTGATTACGCGCCGTCTCCTTTTTTTCATTCCGTGCAATCGGAATACAAGGAAACTCCATCCAAAGGAAAGTTCAAAC
ACACGATCACTTATCATAAATTAGAATATTCGGTTCATCTCGTGAATTTAAAAGAACCAAATCAGATCCAATCGTTATTA
GCTGGAAATGATCTCGAATCCAAATGGGTAGAAGTATCGGATTTAGAATCTGAGTTCCCATCTTCCCTCGCAAAGAAAGT
AAAAAAGATTTTGCTTTACTAA

Upstream 100 bases:

>100_bases
AATTCTTCCGTTCTACACGTTTGGACTATGATTTAGTTCGATACACGGAAGAGGGAAGTTGTAATACCTACCTCCGTTAT
AGCATCCAAGATTCGGACAC

Downstream 100 bases:

>100_bases
TTCAAAAATACCTCTTACGATTTTTTGGCTATGGCAGAGATCATCATTTGGTTAGAGTTATTCATTTCCAAAATCCCTTT
GCCAATCTTAGAAGTCTGGG

Product: putative A/G-specific DNA glycosylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MNPQKKLRDWYLLHKRDLPFRKKKQAYPIWISEVMLQQTRVAAMLPLFENFVNRFPNPESLAKATEEEVLSFWKGLGYYS
RARNIRKAAIQIVQQYNGSFPKDLDSVLKLPGIGNYTARAILSISYDLPLAVLDGNVKRVLSRYYGYTKNILGPQAEKEL
QLKADGFLNLDFPGDHNQAVMELGATICLPESPKCLVCPLMDGCYARIHGKTKEIPLREKKQKQVLLTGEIWVIQQKNLI
LLIKEKKNRFLKGMFHLPSGFLGEIPNSDYAPSPFFHSVQSEYKETPSKGKFKHTITYHKLEYSVHLVNLKEPNQIQSLL
AGNDLESKWVEVSDLESEFPSSLAKKVKKILLY

Sequences:

>Translated_353_residues
MNPQKKLRDWYLLHKRDLPFRKKKQAYPIWISEVMLQQTRVAAMLPLFENFVNRFPNPESLAKATEEEVLSFWKGLGYYS
RARNIRKAAIQIVQQYNGSFPKDLDSVLKLPGIGNYTARAILSISYDLPLAVLDGNVKRVLSRYYGYTKNILGPQAEKEL
QLKADGFLNLDFPGDHNQAVMELGATICLPESPKCLVCPLMDGCYARIHGKTKEIPLREKKQKQVLLTGEIWVIQQKNLI
LLIKEKKNRFLKGMFHLPSGFLGEIPNSDYAPSPFFHSVQSEYKETPSKGKFKHTITYHKLEYSVHLVNLKEPNQIQSLL
AGNDLESKWVEVSDLESEFPSSLAKKVKKILLY
>Mature_353_residues
MNPQKKLRDWYLLHKRDLPFRKKKQAYPIWISEVMLQQTRVAAMLPLFENFVNRFPNPESLAKATEEEVLSFWKGLGYYS
RARNIRKAAIQIVQQYNGSFPKDLDSVLKLPGIGNYTARAILSISYDLPLAVLDGNVKRVLSRYYGYTKNILGPQAEKEL
QLKADGFLNLDFPGDHNQAVMELGATICLPESPKCLVCPLMDGCYARIHGKTKEIPLREKKQKQVLLTGEIWVIQQKNLI
LLIKEKKNRFLKGMFHLPSGFLGEIPNSDYAPSPFFHSVQSEYKETPSKGKFKHTITYHKLEYSVHLVNLKEPNQIQSLL
AGNDLESKWVEVSDLESEFPSSLAKKVKKILLY

Specific function: Involved in the GO system responsible for removing an oxidatively damaged form of guanine (7,8-dihydro-8-oxoguanine, 8- oxo-dGTP) from DNA and the nucleotide pool. 8-oxo-dGTP is inserted opposite dA and dC residues of template DNA with almost equal effici

COG id: COG1194

COG function: function code L; A/G-specific DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HhH domain [H]

Homologues:

Organism=Homo sapiens, GI115298650, Length=234, Percent_Identity=38.034188034188, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI115298654, Length=234, Percent_Identity=38.034188034188, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI115298652, Length=234, Percent_Identity=38.034188034188, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI6912520, Length=234, Percent_Identity=38.034188034188, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI190358497, Length=234, Percent_Identity=38.034188034188, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI115298648, Length=234, Percent_Identity=38.034188034188, Blast_Score=173, Evalue=2e-43,
Organism=Escherichia coli, GI1789331, Length=255, Percent_Identity=41.1764705882353, Blast_Score=190, Evalue=1e-49,
Organism=Escherichia coli, GI1787920, Length=150, Percent_Identity=29.3333333333333, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR023170
- InterPro:   IPR005760
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00730 HhH-GPD [H]

EC number: 3.2.2.-

Molecular weight: Translated: 40515; Mature: 40515

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: PS00445 FGGY_KINASES_2 ; PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPQKKLRDWYLLHKRDLPFRKKKQAYPIWISEVMLQQTRVAAMLPLFENFVNRFPNPES
CCCHHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LAKATEEEVLSFWKGLGYYSRARNIRKAAIQIVQQYNGSFPKDLDSVLKLPGIGNYTARA
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCHHHHE
ILSISYDLPLAVLDGNVKRVLSRYYGYTKNILGPQAEKELQLKADGFLNLDFPGDHNQAV
EEEEECCCCEEEECCCHHHHHHHHHCCHHHCCCCCCCCCEEEECCCEEEECCCCCCCHHH
MELGATICLPESPKCLVCPLMDGCYARIHGKTKEIPLREKKQKQVLLTGEIWVIQQKNLI
HHCCCEEECCCCCCEEEEECHHCHHHHHCCCCCCCCCCCCCCCEEEEECEEEEEECCCEE
LLIKEKKNRFLKGMFHLPSGFLGEIPNSDYAPSPFFHSVQSEYKETPSKGKFKHTITYHK
EEEECHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEEE
LEYSVHLVNLKEPNQIQSLLAGNDLESKWVEVSDLESEFPSSLAKKVKKILLY
EEEEEEEEECCCCHHHHHHHCCCCCHHHHEEHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNPQKKLRDWYLLHKRDLPFRKKKQAYPIWISEVMLQQTRVAAMLPLFENFVNRFPNPES
CCCHHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LAKATEEEVLSFWKGLGYYSRARNIRKAAIQIVQQYNGSFPKDLDSVLKLPGIGNYTARA
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCHHHHE
ILSISYDLPLAVLDGNVKRVLSRYYGYTKNILGPQAEKELQLKADGFLNLDFPGDHNQAV
EEEEECCCCEEEECCCHHHHHHHHHCCHHHCCCCCCCCCEEEECCCEEEECCCCCCCHHH
MELGATICLPESPKCLVCPLMDGCYARIHGKTKEIPLREKKQKQVLLTGEIWVIQQKNLI
HHCCCEEECCCCCCEEEEECHHCHHHHHCCCCCCCCCCCCCCCEEEEECEEEEEECCCEE
LLIKEKKNRFLKGMFHLPSGFLGEIPNSDYAPSPFFHSVQSEYKETPSKGKFKHTITYHK
EEEECHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEEE
LEYSVHLVNLKEPNQIQSLLAGNDLESKWVEVSDLESEFPSSLAKKVKKILLY
EEEEEEEEECCCCHHHHHHHCCCCCHHHHEEHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 4Fe-4S Cluster [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Hydrolysing N-glycosyl compounds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8946165; 9384377 [H]