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The map label for this gene is cysT [H]

Identifier: 183220583

GI number: 183220583

Start: 1235136

End: 1235966

Strand: Reverse

Name: cysT [H]

Synonym: LEPBI_I1187

Alternate gene names: 183220583

Gene position: 1235966-1235136 (Counterclockwise)

Preceding gene: 183220584

Following gene: 183220582

Centisome position: 34.34

GC content: 41.16

Gene sequence:

>831_bases
ATGATGCAAATTAAAACGCGGCCGTACAAAAAAATCCATTTTGGAATCAGTTTAGGATTAACTGTCTTTTATTCGTCCGC
CATTGTCATCATACCACTTCTTGGACTTTTGATCCATTCTTTGGGAATTGGAATCTCTGGAATCCTGGAAGTGTTTACCG
ATGAACGCATTCGATCTGCTTTGTATTTGAGTTTCACTGTAGGTTTTGTTTCTGCCCTACTGAACCTCTTCATTGGTTTT
TTATTTGCTTGGGTCCTTGTTCGTTATCAGTTTCCGTTCAAAAAATTTTTTGATACACTCATAGATCTACCTTTCACTTT
ACCCACAGCGGTTGCCGGGATTGCACTGACGACAATTTACTCCGAGACAGGTATCATTGGTTCCTTTTTTGCCGAGTGGG
GGATTCAAATTGCTTATACACCAGTCGGCATTGTGATCGCACTGGTATTCATTGGGTTTCCCTTTGTGGTAAGAACAGTC
CAACCTGTGATCGAAGAACTTCCGAAAGAATTGGAAGAAAGTGCTCGTTGCCTTGGTGCGACTCCCTTCCAAACATTTAC
GAGAGTATTATTACCTGAACTTTGGCCGTCACTTCTTGCGGGAACGGGAATGGCGTTTGCACGTAGCATAGGGGAATATG
GTTCAGTCGTTTTTATTTCAGGGAACCTTCCTGGCAAAACAGAAATTTTGCCCCTACTCATTGTCACCAAACTAGAACAG
TACGAATATGAGAAGGCAACATCCATTGCGATTGTGATGTTACTCTCTTCGTTTTTCTTTATGTTTCTTATTAATTTATT
ACAAGAACGGGCTTCGAAAAAACTCACATGA

Upstream 100 bases:

>100_bases
ATTATTTTCTCTATCCGATTTAGGTGAAACTTGGGACTCTGCACAGAAAAAACATTTTGCAGATGGTGGATTATTTGATG
CCATCTACAAAACCAAATAA

Downstream 100 bases:

>100_bases
AATTGAAACTTGTTCCCTTCTTACTGATAATTATCGCATACACGTTGTTTGCTGTATTGTTAATTTTGCCGATTTATACA
GTCTTTTCTGAGGCTTTTTC

Product: sulfate ABC transporter permease

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MMQIKTRPYKKIHFGISLGLTVFYSSAIVIIPLLGLLIHSLGIGISGILEVFTDERIRSALYLSFTVGFVSALLNLFIGF
LFAWVLVRYQFPFKKFFDTLIDLPFTLPTAVAGIALTTIYSETGIIGSFFAEWGIQIAYTPVGIVIALVFIGFPFVVRTV
QPVIEELPKELEESARCLGATPFQTFTRVLLPELWPSLLAGTGMAFARSIGEYGSVVFISGNLPGKTEILPLLIVTKLEQ
YEYEKATSIAIVMLLSSFFFMFLINLLQERASKKLT

Sequences:

>Translated_276_residues
MMQIKTRPYKKIHFGISLGLTVFYSSAIVIIPLLGLLIHSLGIGISGILEVFTDERIRSALYLSFTVGFVSALLNLFIGF
LFAWVLVRYQFPFKKFFDTLIDLPFTLPTAVAGIALTTIYSETGIIGSFFAEWGIQIAYTPVGIVIALVFIGFPFVVRTV
QPVIEELPKELEESARCLGATPFQTFTRVLLPELWPSLLAGTGMAFARSIGEYGSVVFISGNLPGKTEILPLLIVTKLEQ
YEYEKATSIAIVMLLSSFFFMFLINLLQERASKKLT
>Mature_276_residues
MMQIKTRPYKKIHFGISLGLTVFYSSAIVIIPLLGLLIHSLGIGISGILEVFTDERIRSALYLSFTVGFVSALLNLFIGF
LFAWVLVRYQFPFKKFFDTLIDLPFTLPTAVAGIALTTIYSETGIIGSFFAEWGIQIAYTPVGIVIALVFIGFPFVVRTV
QPVIEELPKELEESARCLGATPFQTFTRVLLPELWPSLLAGTGMAFARSIGEYGSVVFISGNLPGKTEILPLLIVTKLEQ
YEYEKATSIAIVMLLSSFFFMFLINLLQERASKKLT

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=226, Percent_Identity=45.575221238938, Blast_Score=210, Evalue=9e-56,
Organism=Escherichia coli, GI87082099, Length=274, Percent_Identity=30.2919708029197, Blast_Score=147, Evalue=9e-37,
Organism=Escherichia coli, GI1786980, Length=230, Percent_Identity=33.9130434782609, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1787369, Length=223, Percent_Identity=28.6995515695067, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786254, Length=229, Percent_Identity=25.764192139738, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787081, Length=226, Percent_Identity=26.9911504424779, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30676; Mature: 30676

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MMQIKTRPYKKIHFGISLGLTVFYSSAIVIIPLLGLLIHSLGIGISGILEVFTDERIRSA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LYLSFTVGFVSALLNLFIGFLFAWVLVRYQFPFKKFFDTLIDLPFTLPTAVAGIALTTIY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
SETGIIGSFFAEWGIQIAYTPVGIVIALVFIGFPFVVRTVQPVIEELPKELEESARCLGA
HHCCHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TPFQTFTRVLLPELWPSLLAGTGMAFARSIGEYGSVVFISGNLPGKTEILPLLIVTKLEQ
CHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHH
YEYEKATSIAIVMLLSSFFFMFLINLLQERASKKLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1708375 [H]