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The map label for this gene is cysN [H]

Identifier: 183220578

GI number: 183220578

Start: 1230329

End: 1231561

Strand: Reverse

Name: cysN [H]

Synonym: LEPBI_I1182

Alternate gene names: 183220578

Gene position: 1231561-1230329 (Counterclockwise)

Preceding gene: 183220579

Following gene: 183220575

Centisome position: 34.21

GC content: 41.93

Gene sequence:

>1233_bases
ATGGATATTTTACGATTTATCACAGCAGGAAGTGTGGACGATGGGAAATCAACTCTCATCGGACGTTTGTTATACGATAG
TAAGTCCATTTTCCAAGACCAGATGGAAGCGATCGAAAGAGCAGGCCAAGTGAATGGACAAATCAATTTAGCCCTACTCA
CCGATGGTCTCAAAGCAGAAAGAGAACAAGGCATCACAATCGATGTCGCGTACAAATACTTTTCGACTCCTAAACGTAAG
TTCATCATCGCAGATGCACCAGGCCATGTCCAATACACAAGGAATATGGTGACAGGTGCCTCCAATTCAGACCTTGCCAT
CATCCTCATTGATGCAAGAAAGGGAGTGATCGAACAAACTTACAGACATTCATACATCGTTTCTTTATTACGACTCCCAT
ATGTGGTCGTTTGCGTGAACAAAATGGATTTGGTGGATTTTTCTGAAGAAGTGTTTTTGAATATCAAACAACAATATTTG
GAATTTGCAAAAGATTTGGATTTAAAATCCATTCACTTCCTTCCCATCTCCGCTTTAAATGGAGACAATGTTGTGGATAC
ATCCCCATCTATGCCATGGTGGAAAGGAAAATCCTTACTTCACTTTTTGGAGGAAATTGAGATCCATACGGAAGAAGAAT
CCCCTGCTCCGCGATTCCCAGTCCAAAATGTAATTCGTCCACAAACAACAGAATACCATGATTACAGAGGTTATGCGGGA
CAAATCAGAAGTGGCCATTTTACTGTGGGTGATTCCATCACAGTCCTACCAAGTGGACTCACTTCCAAAATCAAAGCCAT
CGATAGTTTTGATGGGCCACTCACAACAGCCTATGCACCAATGTCTATTACCATTCGATTGGAAGATGAGATTGATGTGA
GCCGAGGAGATATGCTTGTTGTCACGGGTAAAGAACCCAAAATTTCCCAAGATTTGGAAGCATATATTTGTTGGATGGAC
CAAAAAGTCCTCACTCCAGGATCCAAATATTTACTCCGCCAAACGACAAACGCAGTGAAGGCATCCATCCGTTCTTTGGA
ATTTAGAGTGGAAACAAGCACTCACGAAAAAAAAGAACAAACGAGTCTTGGTTTGAATGAAATTGGAAAAGTAACCATTC
GGACGGCAAAACCTGTCGCTTACGATCCGTATTCCAAAATTAGGGGCACAGGGAGTTTTATCTTAGTCGATGAAGGAACC
AACCAAACCGTTGCGGCAGGAATGTTATTGTAG

Upstream 100 bases:

>100_bases
GTGAGATCCAAATTTCCCGTACAACGGAAAGAGGTTCTCGATTGGATGACAAACGATCGGAAGCTGCAATGGAAGATAGA
AAAAAAGGTGGGTATTTTTA

Downstream 100 bases:

>100_bases
AGAGATGGCTTCCAAAATCGAAATTGATTTTCGATTTAACTCGTTTAGTAAAATCAATCGAAAGGAGTTCGAAATCAATT
AATTTGTGATCTGCACTTTG

Product: ATP:sulfate adenylyltransferase

Products: NA

Alternate protein names: Sulfate adenylyltransferase subunit 1; ATP-sulfurylase large subunit; Sulfate adenylate transferase; SAT; Adenylyl-sulfate kinase; APS kinase; ATP adenosine-5'-phosphosulfate 3'-phosphotransferase [H]

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MDILRFITAGSVDDGKSTLIGRLLYDSKSIFQDQMEAIERAGQVNGQINLALLTDGLKAEREQGITIDVAYKYFSTPKRK
FIIADAPGHVQYTRNMVTGASNSDLAIILIDARKGVIEQTYRHSYIVSLLRLPYVVVCVNKMDLVDFSEEVFLNIKQQYL
EFAKDLDLKSIHFLPISALNGDNVVDTSPSMPWWKGKSLLHFLEEIEIHTEEESPAPRFPVQNVIRPQTTEYHDYRGYAG
QIRSGHFTVGDSITVLPSGLTSKIKAIDSFDGPLTTAYAPMSITIRLEDEIDVSRGDMLVVTGKEPKISQDLEAYICWMD
QKVLTPGSKYLLRQTTNAVKASIRSLEFRVETSTHEKKEQTSLGLNEIGKVTIRTAKPVAYDPYSKIRGTGSFILVDEGT
NQTVAAGMLL

Sequences:

>Translated_410_residues
MDILRFITAGSVDDGKSTLIGRLLYDSKSIFQDQMEAIERAGQVNGQINLALLTDGLKAEREQGITIDVAYKYFSTPKRK
FIIADAPGHVQYTRNMVTGASNSDLAIILIDARKGVIEQTYRHSYIVSLLRLPYVVVCVNKMDLVDFSEEVFLNIKQQYL
EFAKDLDLKSIHFLPISALNGDNVVDTSPSMPWWKGKSLLHFLEEIEIHTEEESPAPRFPVQNVIRPQTTEYHDYRGYAG
QIRSGHFTVGDSITVLPSGLTSKIKAIDSFDGPLTTAYAPMSITIRLEDEIDVSRGDMLVVTGKEPKISQDLEAYICWMD
QKVLTPGSKYLLRQTTNAVKASIRSLEFRVETSTHEKKEQTSLGLNEIGKVTIRTAKPVAYDPYSKIRGTGSFILVDEGT
NQTVAAGMLL
>Mature_410_residues
MDILRFITAGSVDDGKSTLIGRLLYDSKSIFQDQMEAIERAGQVNGQINLALLTDGLKAEREQGITIDVAYKYFSTPKRK
FIIADAPGHVQYTRNMVTGASNSDLAIILIDARKGVIEQTYRHSYIVSLLRLPYVVVCVNKMDLVDFSEEVFLNIKQQYL
EFAKDLDLKSIHFLPISALNGDNVVDTSPSMPWWKGKSLLHFLEEIEIHTEEESPAPRFPVQNVIRPQTTEYHDYRGYAG
QIRSGHFTVGDSITVLPSGLTSKIKAIDSFDGPLTTAYAPMSITIRLEDEIDVSRGDMLVVTGKEPKISQDLEAYICWMD
QKVLTPGSKYLLRQTTNAVKASIRSLEFRVETSTHEKKEQTSLGLNEIGKVTIRTAKPVAYDPYSKIRGTGSFILVDEGT
NQTVAAGMLL

Specific function: APS kinase catalyzes the synthesis of activated sulfate [H]

COG id: COG2895

COG function: function code P; GTPases - Sulfate adenylate transferase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the APS kinase family [H]

Homologues:

Organism=Homo sapiens, GI223555963, Length=426, Percent_Identity=29.5774647887324, Blast_Score=162, Evalue=8e-40,
Organism=Homo sapiens, GI5729864, Length=426, Percent_Identity=29.5774647887324, Blast_Score=161, Evalue=9e-40,
Organism=Homo sapiens, GI194018522, Length=424, Percent_Identity=28.3018867924528, Blast_Score=152, Evalue=5e-37,
Organism=Homo sapiens, GI194097354, Length=424, Percent_Identity=28.3018867924528, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI194018520, Length=424, Percent_Identity=28.3018867924528, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI4503475, Length=437, Percent_Identity=27.4599542334096, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI46094014, Length=424, Percent_Identity=27.122641509434, Blast_Score=143, Evalue=3e-34,
Organism=Homo sapiens, GI4503471, Length=437, Percent_Identity=27.0022883295195, Blast_Score=142, Evalue=8e-34,
Organism=Homo sapiens, GI34147630, Length=162, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=9e-15,
Organism=Escherichia coli, GI1789108, Length=412, Percent_Identity=48.7864077669903, Blast_Score=390, Evalue=1e-109,
Organism=Escherichia coli, GI1789737, Length=137, Percent_Identity=30.6569343065693, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1790412, Length=137, Percent_Identity=30.6569343065693, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI2367247, Length=306, Percent_Identity=24.1830065359477, Blast_Score=72, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17552884, Length=442, Percent_Identity=28.7330316742081, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17569207, Length=442, Percent_Identity=28.7330316742081, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI32566629, Length=428, Percent_Identity=26.1682242990654, Blast_Score=144, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI32566303, Length=435, Percent_Identity=28.0459770114943, Blast_Score=141, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI115532067, Length=426, Percent_Identity=25.8215962441315, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI115532065, Length=426, Percent_Identity=25.8215962441315, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI32566301, Length=142, Percent_Identity=36.6197183098592, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17556456, Length=359, Percent_Identity=26.4623955431755, Blast_Score=81, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI25141371, Length=176, Percent_Identity=28.9772727272727, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6325337, Length=288, Percent_Identity=32.9861111111111, Blast_Score=154, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6319594, Length=288, Percent_Identity=32.9861111111111, Blast_Score=154, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6322937, Length=275, Percent_Identity=32, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6320377, Length=432, Percent_Identity=28.9351851851852, Blast_Score=128, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6324761, Length=165, Percent_Identity=32.7272727272727, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI45550900, Length=432, Percent_Identity=28.2407407407407, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24652838, Length=438, Percent_Identity=26.7123287671233, Blast_Score=144, Evalue=9e-35,
Organism=Drosophila melanogaster, GI17137572, Length=438, Percent_Identity=26.7123287671233, Blast_Score=144, Evalue=9e-35,
Organism=Drosophila melanogaster, GI45553807, Length=434, Percent_Identity=26.2672811059908, Blast_Score=142, Evalue=6e-34,
Organism=Drosophila melanogaster, GI45553816, Length=434, Percent_Identity=26.2672811059908, Blast_Score=142, Evalue=6e-34,
Organism=Drosophila melanogaster, GI24651721, Length=434, Percent_Identity=26.2672811059908, Blast_Score=142, Evalue=6e-34,
Organism=Drosophila melanogaster, GI17864154, Length=434, Percent_Identity=26.2672811059908, Blast_Score=142, Evalue=6e-34,
Organism=Drosophila melanogaster, GI17137380, Length=424, Percent_Identity=25.7075471698113, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI281363316, Length=202, Percent_Identity=26.7326732673267, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI17864358, Length=202, Percent_Identity=26.7326732673267, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19921738, Length=170, Percent_Identity=30.5882352941176, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002891
- InterPro:   IPR000795
- InterPro:   IPR011779
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF01583 APS_kinase; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: =2.7.7.4; =2.7.1.25 [H]

Molecular weight: Translated: 45780; Mature: 45780

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDILRFITAGSVDDGKSTLIGRLLYDSKSIFQDQMEAIERAGQVNGQINLALLTDGLKAE
CCCEEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHH
REQGITIDVAYKYFSTPKRKFIIADAPGHVQYTRNMVTGASNSDLAIILIDARKGVIEQT
HHCCCEEEEEEHHHCCCCEEEEEECCCCCEEEEHHEECCCCCCCEEEEEEECCCCHHHHH
YRHSYIVSLLRLPYVVVCVNKMDLVDFSEEVFLNIKQQYLEFAKDLDLKSIHFLPISALN
HHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECC
GDNVVDTSPSMPWWKGKSLLHFLEEIEIHTEEESPAPRFPVQNVIRPQTTEYHDYRGYAG
CCCEECCCCCCCCCCCHHHHHHHHHHHEECCCCCCCCCCCHHHCCCCCCCCCCCCCCCCC
QIRSGHFTVGDSITVLPSGLTSKIKAIDSFDGPLTTAYAPMSITIRLEDEIDVSRGDMLV
CCCCCCEEECCCEEEECCCHHHHHHHHHCCCCCCEEEECCEEEEEEEECCCCCCCCCEEE
VTGKEPKISQDLEAYICWMDQKVLTPGSKYLLRQTTNAVKASIRSLEFRVETSTHEKKEQ
EECCCCCCCCCCEEEEEEECCEECCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHH
TSLGLNEIGKVTIRTAKPVAYDPYSKIRGTGSFILVDEGTNQTVAAGMLL
HHCCHHHCCEEEEEECCCCEECCHHHHCCCCCEEEEECCCCCEECCCCCC
>Mature Secondary Structure
MDILRFITAGSVDDGKSTLIGRLLYDSKSIFQDQMEAIERAGQVNGQINLALLTDGLKAE
CCCEEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHH
REQGITIDVAYKYFSTPKRKFIIADAPGHVQYTRNMVTGASNSDLAIILIDARKGVIEQT
HHCCCEEEEEEHHHCCCCEEEEEECCCCCEEEEHHEECCCCCCCEEEEEEECCCCHHHHH
YRHSYIVSLLRLPYVVVCVNKMDLVDFSEEVFLNIKQQYLEFAKDLDLKSIHFLPISALN
HHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECC
GDNVVDTSPSMPWWKGKSLLHFLEEIEIHTEEESPAPRFPVQNVIRPQTTEYHDYRGYAG
CCCEECCCCCCCCCCCHHHHHHHHHHHEECCCCCCCCCCCHHHCCCCCCCCCCCCCCCCC
QIRSGHFTVGDSITVLPSGLTSKIKAIDSFDGPLTTAYAPMSITIRLEDEIDVSRGDMLV
CCCCCCEEECCCEEEECCCHHHHHHHHHCCCCCCEEEECCEEEEEEEECCCCCCCCCEEE
VTGKEPKISQDLEAYICWMDQKVLTPGSKYLLRQTTNAVKASIRSLEFRVETSTHEKKEQ
EECCCCCCCCCCEEEEEEECCEECCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHH
TSLGLNEIGKVTIRTAKPVAYDPYSKIRGTGSFILVDEGTNQTVAAGMLL
HHCCHHHCCEEEEEECCCCEECCHHHHCCCCCEEEEECCCCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]