Click here to switch to the map view.

The map label for this gene is hemB [H]

Identifier: 183220565

GI number: 183220565

Start: 1217903

End: 1218856

Strand: Reverse

Name: hemB [H]

Synonym: LEPBI_I1169

Alternate gene names: 183220565

Gene position: 1218856-1217903 (Counterclockwise)

Preceding gene: 183220566

Following gene: 183220564

Centisome position: 33.86

GC content: 39.1

Gene sequence:

>954_bases
ATGAAAAAACAAACACTTCGATTACGTTCCCACAAGTCACTCCGTCATCTGAGCGAATCAGGTTCCTTAAATGTGAACAA
AATGATCCAACCTTTATTCCTTGTGGAAGGTCTTACCGAAAAAGAACCGATCAAAGGGTTACCAGATGTTTACCGCGATA
CGAACCAAACCATCTTCAAACAAATTGAGTCTGATTTAAAATCAGGTGTCTCGCAGTTTTTGCTATTTATGGTTCCAAAC
GAAAAATCGGACACCAGTTTTTCAAAAGAGTTTTACCATTCAAATATTAGTGCCATAAAAAAAACATTCCCTGAAATGTT
TTTATGGTTAGATACTTGTATCTGCTCAGTGACAACATCCGGACATTGTTGCCACTTCCATCCTAAAGGTAACATTGATT
TAAAATTAACATTAAATCGATTGTCTGAACTAGCATTGATTTATGCAGATGCGGGTGCTGACGGTATTGCACCTAGTGAT
ATGATGGATGGAAGGGTTGCATCACATAGAAAGATTCTCGATGAAAACAACCATTCCCATGTTCCCATCATGAGTTATTC
TACAAAATTCAAAAGTCATTTTTACGGTCCCTTCCGTGGTGCTGCCGACTCCTCACCGCAGTTTGGTGATAGAAGTGGAT
ACCAATTGGATGTTAGAGACAGAGAAACTGCAATCCAAACTTCCATTCGAGACAAAGAAGAAGGTGCGGATTTATTAATG
GTAAAACCAGGGATGACTTCTATTGATTTGATTGGTCCTATCAAAGATAAAACAGGACTCCCTACTGGTGCTTACCAAGT
GAGTGGCGAGTATGCAAGTCTCGTTTACTTAGCAAAAGAAGGTTTTCTCAATTTTGAAGATGGTTTAAGAGAAACATGGG
ACGTGTTTCGCAGAGCCGGATCTTCATTTTTAATCACGTATGGTGCAAGGATTGCACAAAGGTTGTATTCATGA

Upstream 100 bases:

>100_bases
CGTTTCGAAAAATCAAACACGCACTAGGTGAAAATGGTTCCAAAAACAAAGTATTTGTTTCCCTGTCATTTGATTCTTGG
GTGGAGAGGTACATCAAACC

Downstream 100 bases:

>100_bases
ATTCGGAAACATTATTCCAGAGATCAAAACAAGTGGTTCCAGGAGGAGTCCACAGTCCTGTCAGGTCATTCTCCTCAGTA
GGTGGGACTCCCATTTTTTT

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MKKQTLRLRSHKSLRHLSESGSLNVNKMIQPLFLVEGLTEKEPIKGLPDVYRDTNQTIFKQIESDLKSGVSQFLLFMVPN
EKSDTSFSKEFYHSNISAIKKTFPEMFLWLDTCICSVTTSGHCCHFHPKGNIDLKLTLNRLSELALIYADAGADGIAPSD
MMDGRVASHRKILDENNHSHVPIMSYSTKFKSHFYGPFRGAADSSPQFGDRSGYQLDVRDRETAIQTSIRDKEEGADLLM
VKPGMTSIDLIGPIKDKTGLPTGAYQVSGEYASLVYLAKEGFLNFEDGLRETWDVFRRAGSSFLITYGARIAQRLYS

Sequences:

>Translated_317_residues
MKKQTLRLRSHKSLRHLSESGSLNVNKMIQPLFLVEGLTEKEPIKGLPDVYRDTNQTIFKQIESDLKSGVSQFLLFMVPN
EKSDTSFSKEFYHSNISAIKKTFPEMFLWLDTCICSVTTSGHCCHFHPKGNIDLKLTLNRLSELALIYADAGADGIAPSD
MMDGRVASHRKILDENNHSHVPIMSYSTKFKSHFYGPFRGAADSSPQFGDRSGYQLDVRDRETAIQTSIRDKEEGADLLM
VKPGMTSIDLIGPIKDKTGLPTGAYQVSGEYASLVYLAKEGFLNFEDGLRETWDVFRRAGSSFLITYGARIAQRLYS
>Mature_317_residues
MKKQTLRLRSHKSLRHLSESGSLNVNKMIQPLFLVEGLTEKEPIKGLPDVYRDTNQTIFKQIESDLKSGVSQFLLFMVPN
EKSDTSFSKEFYHSNISAIKKTFPEMFLWLDTCICSVTTSGHCCHFHPKGNIDLKLTLNRLSELALIYADAGADGIAPSD
MMDGRVASHRKILDENNHSHVPIMSYSTKFKSHFYGPFRGAADSSPQFGDRSGYQLDVRDRETAIQTSIRDKEEGADLLM
VKPGMTSIDLIGPIKDKTGLPTGAYQVSGEYASLVYLAKEGFLNFEDGLRETWDVFRRAGSSFLITYGARIAQRLYS

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=306, Percent_Identity=39.2156862745098, Blast_Score=210, Evalue=1e-54,
Organism=Escherichia coli, GI87081728, Length=307, Percent_Identity=37.4592833876222, Blast_Score=197, Evalue=7e-52,
Organism=Saccharomyces cerevisiae, GI6321398, Length=309, Percent_Identity=33.6569579288026, Blast_Score=169, Evalue=4e-43,
Organism=Drosophila melanogaster, GI21358291, Length=305, Percent_Identity=38.0327868852459, Blast_Score=204, Evalue=4e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 35611; Mature: 35611

Theoretical pI: Translated: 8.01; Mature: 8.01

Prosite motif: PS00022 EGF_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKQTLRLRSHKSLRHLSESGSLNVNKMIQPLFLVEGLTEKEPIKGLPDVYRDTNQTIFK
CCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCHHHHHH
QIESDLKSGVSQFLLFMVPNEKSDTSFSKEFYHSNISAIKKTFPEMFLWLDTCICSVTTS
HHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GHCCHFHPKGNIDLKLTLNRLSELALIYADAGADGIAPSDMMDGRVASHRKILDENNHSH
CCEEEECCCCCEEEEEEHHHHHCEEEEEECCCCCCCCCCHHHCCHHHHHHHHHCCCCCCC
VPIMSYSTKFKSHFYGPFRGAADSSPQFGDRSGYQLDVRDRETAIQTSIRDKEEGADLLM
CEEEECCHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHCCHHCCCCEEE
VKPGMTSIDLIGPIKDKTGLPTGAYQVSGEYASLVYLAKEGFLNFEDGLRETWDVFRRAG
ECCCCCEEEEEECCCCCCCCCCCCEEECCCCEEEEEEHHCCCCCHHHHHHHHHHHHHHCC
SSFLITYGARIAQRLYS
CCEEEEHHHHHHHHHCC
>Mature Secondary Structure
MKKQTLRLRSHKSLRHLSESGSLNVNKMIQPLFLVEGLTEKEPIKGLPDVYRDTNQTIFK
CCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCHHHHHH
QIESDLKSGVSQFLLFMVPNEKSDTSFSKEFYHSNISAIKKTFPEMFLWLDTCICSVTTS
HHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GHCCHFHPKGNIDLKLTLNRLSELALIYADAGADGIAPSDMMDGRVASHRKILDENNHSH
CCEEEECCCCCEEEEEEHHHHHCEEEEEECCCCCCCCCCHHHCCHHHHHHHHHCCCCCCC
VPIMSYSTKFKSHFYGPFRGAADSSPQFGDRSGYQLDVRDRETAIQTSIRDKEEGADLLM
CEEEECCHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHCCHHCCCCEEE
VKPGMTSIDLIGPIKDKTGLPTGAYQVSGEYASLVYLAKEGFLNFEDGLRETWDVFRRAG
ECCCCCEEEEEECCCCCCCCCCCCEEECCCCEEEEEEHHCCCCCHHHHHHHHHHHHHHCC
SSFLITYGARIAQRLYS
CCEEEEHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]