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The map label for this gene is rsbU [H]

Identifier: 183220465

GI number: 183220465

Start: 1103047

End: 1104807

Strand: Reverse

Name: rsbU [H]

Synonym: LEPBI_I1062

Alternate gene names: 183220465

Gene position: 1104807-1103047 (Counterclockwise)

Preceding gene: 183220471

Following gene: 183220463

Centisome position: 30.69

GC content: 39.64

Gene sequence:

>1761_bases
ATGACCGATCCGCAAACTAGTCTTAGTAAATTCCGTAGCCTACTTCATATTTCATCGATTCTGAATGCAAATTTAGACCT
GCACCAACTTCTCCCTCTCATCATGTTGTATTCCAAAGATTTGTTAGAGGCGGAGGCAAGTTCCTTATTCTTACTAGAAA
ACGATGAATTTTTGTATTGTGAAGTGGCCTTGGGAGAAAAAGGGGAAATCATCCAGGAATATGCACGTTTGGAATTTGGC
GAAGGCATCGTAGGTATGGTTGCCAAAGAAAAAAAACCAATCGCACTCGAAGATGCCTACAAAGACCCAAGATTCAATCC
GAGTATGGACAAACGTACGGGATTCAAAACCAAGTCTCTCATTTGTGTCCCTCTCTTTGTCGAAGAAAGGCTCATTGGAA
CACTTGAAGTCATCAACAAAACCAATGATCGAATTTTTAATGATGCCGATTTAGAATATTTGATTTCTCTATCAGAAGTT
GCTGCCACAGCCATTCAAAATGCCAATACGAAAGATAGTTTGGACAAACGGATTTTAGAACTTTCCTTACTCTATGAATT
TGAAAAACTATCGGTTTCCGAAAAAAGTTTAAATGAACTAGGAAAGTGGATGTTAAACCGAGTTTTGGAATACTTAGGGG
CAAGCTCTGGAACCATCTACCTCGCAAACACAACCCAACAAGAGTTAGGTATTCTTGCGGCAAAAGGAATCCCGGAAGAT
GCGTATGACCAAATCAAAGTTCCTTTTGGAAAAGGGGTTTCGGGTTGGGTCGCTGAAAAAAAAGAAAGTTTACTCATCCA
TAATTTGGATATGGATCCAAGGTACAATCAACTTTCCCCATTTCGATTTGAATCCAAATCACTCATCTCAGCACCACTCA
TTTACCAAAACGAACTTTTGGGAGTGATTAGCATCAATAACAAACTCTCAGGGTATGCCTTCCAACATTCTGATTTAGAC
CTACTCACAAACATTGCAGCAAGACTCAGTAACACGATTAAAAATGCCCAACTCTTCCACCAAATTGTGGATACAGGAAA
GGAGCTGAACCGTGCCAAAAACATCATGCAAAAAATCATGCCATCGGTTTTGCCAGAAACCAAAGAATTGAGTTATGGAG
TCTCTCACATTCCACTCGAACAAGTGGGAGGTGATTTTTATGATGTCACAGAAATTGATGAATTTCAATATTCCATTTTG
ATTGCGGATATATCTGGACACGGACTTTCGGCTGCCGTACTTGCAGCGATGTCCCATATGGTTCTCAAAAATTTTGAACC
CGATATCAAACTAAGTCCTTCTCTATTTTTAACCACACTCAATCATATGTTATTTGGGAAACTGGCAGGTAATTTCTTAA
CTGCATTTTATGGAATCATCAATTTAAAAGAAAATTCCATCCTCTGTGCCAATGCAGGCCACCATGCACCTTTTTTGATG
AAAGGGACGAGTGCCGATGCCTCTGCTTTGGATGTGAAAGGAAAAATCCTTGGTCTCATTCCCGATCTCTATTATGAGGA
AAAAAAGTTTTCGTTTGGATCCGGAGACCGTTTGGTATTGTATACCGACGGAATTACCGAACATATGTCAAAAGACCATA
ACAAACGTTATGACGATGAATTGTTCCAAAAGGCAATTATAAAAGGGAAATCAAAGTCAGCACAAGATTGTGCCAACTCA
CTCATCCAAGAAGCAAAAGACTATGTAAATTCCAATGAGTTTGCCGATGATGTGACGATCCTGATCGTCGATCGAAAATG
A

Upstream 100 bases:

>100_bases
AAACGGTTCTTTTTTCCCTTTCCCTAAATCGGCCATAAAAAGACCAAAATTTCTAAATCGACGAAAGGGAATCCATTGGA
AAGATTGGTTTGTTGCCTGT

Downstream 100 bases:

>100_bases
GATTCAGGATTCTCACTGCAGAGAGTCGAATTTCCCTCAAACGAATGGACTCATTTTTAAAGTTCGATGGATTCGTTCCC
TTTGTTGCGTAAGGTTTCGA

Product: putative sigma factor sigB regulation protein RsbU

Products: NA

Alternate protein names: Sigma factor sigB regulation protein rsbU [H]

Number of amino acids: Translated: 586; Mature: 585

Protein sequence:

>586_residues
MTDPQTSLSKFRSLLHISSILNANLDLHQLLPLIMLYSKDLLEAEASSLFLLENDEFLYCEVALGEKGEIIQEYARLEFG
EGIVGMVAKEKKPIALEDAYKDPRFNPSMDKRTGFKTKSLICVPLFVEERLIGTLEVINKTNDRIFNDADLEYLISLSEV
AATAIQNANTKDSLDKRILELSLLYEFEKLSVSEKSLNELGKWMLNRVLEYLGASSGTIYLANTTQQELGILAAKGIPED
AYDQIKVPFGKGVSGWVAEKKESLLIHNLDMDPRYNQLSPFRFESKSLISAPLIYQNELLGVISINNKLSGYAFQHSDLD
LLTNIAARLSNTIKNAQLFHQIVDTGKELNRAKNIMQKIMPSVLPETKELSYGVSHIPLEQVGGDFYDVTEIDEFQYSIL
IADISGHGLSAAVLAAMSHMVLKNFEPDIKLSPSLFLTTLNHMLFGKLAGNFLTAFYGIINLKENSILCANAGHHAPFLM
KGTSADASALDVKGKILGLIPDLYYEEKKFSFGSGDRLVLYTDGITEHMSKDHNKRYDDELFQKAIIKGKSKSAQDCANS
LIQEAKDYVNSNEFADDVTILIVDRK

Sequences:

>Translated_586_residues
MTDPQTSLSKFRSLLHISSILNANLDLHQLLPLIMLYSKDLLEAEASSLFLLENDEFLYCEVALGEKGEIIQEYARLEFG
EGIVGMVAKEKKPIALEDAYKDPRFNPSMDKRTGFKTKSLICVPLFVEERLIGTLEVINKTNDRIFNDADLEYLISLSEV
AATAIQNANTKDSLDKRILELSLLYEFEKLSVSEKSLNELGKWMLNRVLEYLGASSGTIYLANTTQQELGILAAKGIPED
AYDQIKVPFGKGVSGWVAEKKESLLIHNLDMDPRYNQLSPFRFESKSLISAPLIYQNELLGVISINNKLSGYAFQHSDLD
LLTNIAARLSNTIKNAQLFHQIVDTGKELNRAKNIMQKIMPSVLPETKELSYGVSHIPLEQVGGDFYDVTEIDEFQYSIL
IADISGHGLSAAVLAAMSHMVLKNFEPDIKLSPSLFLTTLNHMLFGKLAGNFLTAFYGIINLKENSILCANAGHHAPFLM
KGTSADASALDVKGKILGLIPDLYYEEKKFSFGSGDRLVLYTDGITEHMSKDHNKRYDDELFQKAIIKGKSKSAQDCANS
LIQEAKDYVNSNEFADDVTILIVDRK
>Mature_585_residues
TDPQTSLSKFRSLLHISSILNANLDLHQLLPLIMLYSKDLLEAEASSLFLLENDEFLYCEVALGEKGEIIQEYARLEFGE
GIVGMVAKEKKPIALEDAYKDPRFNPSMDKRTGFKTKSLICVPLFVEERLIGTLEVINKTNDRIFNDADLEYLISLSEVA
ATAIQNANTKDSLDKRILELSLLYEFEKLSVSEKSLNELGKWMLNRVLEYLGASSGTIYLANTTQQELGILAAKGIPEDA
YDQIKVPFGKGVSGWVAEKKESLLIHNLDMDPRYNQLSPFRFESKSLISAPLIYQNELLGVISINNKLSGYAFQHSDLDL
LTNIAARLSNTIKNAQLFHQIVDTGKELNRAKNIMQKIMPSVLPETKELSYGVSHIPLEQVGGDFYDVTEIDEFQYSILI
ADISGHGLSAAVLAAMSHMVLKNFEPDIKLSPSLFLTTLNHMLFGKLAGNFLTAFYGIINLKENSILCANAGHHAPFLMK
GTSADASALDVKGKILGLIPDLYYEEKKFSFGSGDRLVLYTDGITEHMSKDHNKRYDDELFQKAIIKGKSKSAQDCANSL
IQEAKDYVNSNEFADDVTILIVDRK

Specific function: Positive regulator of sigma-B activity. Dephosphorylates rsbV in response to environmental stress conveyed from the rsbXST module [H]

COG id: COG2208

COG function: function code TK; Serine phosphatase RsbU, regulator of sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PP2C-like domain [H]

Homologues:

Organism=Homo sapiens, GI61744435, Length=228, Percent_Identity=28.5087719298246, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI61744430, Length=228, Percent_Identity=28.5087719298246, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI61744432, Length=228, Percent_Identity=28.5087719298246, Blast_Score=87, Evalue=5e-17,
Organism=Homo sapiens, GI194595488, Length=207, Percent_Identity=28.5024154589372, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI5729972, Length=207, Percent_Identity=28.5024154589372, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI116536085, Length=365, Percent_Identity=23.8356164383562, Blast_Score=76, Evalue=1e-13,
Organism=Homo sapiens, GI4505657, Length=165, Percent_Identity=29.0909090909091, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI219802706, Length=165, Percent_Identity=29.0909090909091, Blast_Score=67, Evalue=5e-11,
Organism=Homo sapiens, GI226246591, Length=165, Percent_Identity=29.0909090909091, Blast_Score=67, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI193202333, Length=182, Percent_Identity=26.9230769230769, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI161076955, Length=356, Percent_Identity=24.7191011235955, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI161076953, Length=356, Percent_Identity=24.7191011235955, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI281361762, Length=94, Percent_Identity=41.4893617021277, Blast_Score=76, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017944
- InterPro:   IPR001932
- InterPro:   IPR014787
- InterPro:   IPR010822
- ProDom:   PD685490 [H]

Pfam domain/function: PF08673 RsbU_N; PF07228 SpoIIE [H]

EC number: =3.1.3.3 [H]

Molecular weight: Translated: 65410; Mature: 65279

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDPQTSLSKFRSLLHISSILNANLDLHQLLPLIMLYSKDLLEAEASSLFLLENDEFLYC
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEE
EVALGEKGEIIQEYARLEFGEGIVGMVAKEKKPIALEDAYKDPRFNPSMDKRTGFKTKSL
EEECCCCCHHHHHHHHHHCCCCCEEHHHCCCCCCCHHHCCCCCCCCCCCCHHCCCCCCCE
ICVPLFVEERLIGTLEVINKTNDRIFNDADLEYLISLSEVAATAIQNANTKDSLDKRILE
EEEEHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LSLLYEFEKLSVSEKSLNELGKWMLNRVLEYLGASSGTIYLANTTQQELGILAAKGIPED
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHCEEEECCCCCC
AYDQIKVPFGKGVSGWVAEKKESLLIHNLDMDPRYNQLSPFRFESKSLISAPLIYQNELL
CHHCEECCCCCCCCCHHHHCCCCEEEEECCCCCCCCCCCCCEECCCHHHCCCCEECCCEE
GVISINNKLSGYAFQHSDLDLLTNIAARLSNTIKNAQLFHQIVDTGKELNRAKNIMQKIM
EEEEECCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PSVLPETKELSYGVSHIPLEQVGGDFYDVTEIDEFQYSILIADISGHGLSAAVLAAMSHM
HHHCCCCHHHHCCCCCCCHHHHCCCEEECCCCCCEEEEEEEEECCCCCHHHHHHHHHHHH
VLKNFEPDIKLSPSLFLTTLNHMLFGKLAGNFLTAFYGIINLKENSILCANAGHHAPFLM
HHHCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEECCCCCCCEEE
KGTSADASALDVKGKILGLIPDLYYEEKKFSFGSGDRLVLYTDGITEHMSKDHNKRYDDE
ECCCCCCCCEECCCHHEECCCHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHCCCCCHHH
LFQKAIIKGKSKSAQDCANSLIQEAKDYVNSNEFADDVTILIVDRK
HHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECC
>Mature Secondary Structure 
TDPQTSLSKFRSLLHISSILNANLDLHQLLPLIMLYSKDLLEAEASSLFLLENDEFLYC
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEE
EVALGEKGEIIQEYARLEFGEGIVGMVAKEKKPIALEDAYKDPRFNPSMDKRTGFKTKSL
EEECCCCCHHHHHHHHHHCCCCCEEHHHCCCCCCCHHHCCCCCCCCCCCCHHCCCCCCCE
ICVPLFVEERLIGTLEVINKTNDRIFNDADLEYLISLSEVAATAIQNANTKDSLDKRILE
EEEEHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LSLLYEFEKLSVSEKSLNELGKWMLNRVLEYLGASSGTIYLANTTQQELGILAAKGIPED
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHCEEEECCCCCC
AYDQIKVPFGKGVSGWVAEKKESLLIHNLDMDPRYNQLSPFRFESKSLISAPLIYQNELL
CHHCEECCCCCCCCCHHHHCCCCEEEEECCCCCCCCCCCCCEECCCHHHCCCCEECCCEE
GVISINNKLSGYAFQHSDLDLLTNIAARLSNTIKNAQLFHQIVDTGKELNRAKNIMQKIM
EEEEECCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PSVLPETKELSYGVSHIPLEQVGGDFYDVTEIDEFQYSILIADISGHGLSAAVLAAMSHM
HHHCCCCHHHHCCCCCCCHHHHCCCEEECCCCCCEEEEEEEEECCCCCHHHHHHHHHHHH
VLKNFEPDIKLSPSLFLTTLNHMLFGKLAGNFLTAFYGIINLKENSILCANAGHHAPFLM
HHHCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEECCCCCCCEEE
KGTSADASALDVKGKILGLIPDLYYEEKKFSFGSGDRLVLYTDGITEHMSKDHNKRYDDE
ECCCCCCCCEECCCHHEECCCHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHCCCCCHHH
LFQKAIIKGKSKSAQDCANSLIQEAKDYVNSNEFADDVTILIVDRK
HHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8002610; 8002609; 9384377; 8824586; 9786195; 12499568 [H]