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The map label for this gene is lpdA1 [H]

Identifier: 183220460

GI number: 183220460

Start: 1098379

End: 1099785

Strand: Reverse

Name: lpdA1 [H]

Synonym: LEPBI_I1057

Alternate gene names: 183220460

Gene position: 1099785-1098379 (Counterclockwise)

Preceding gene: 183220461

Following gene: 183220459

Centisome position: 30.55

GC content: 47.76

Gene sequence:

>1407_bases
ATGGAACAATACGATATCATTGTGATTGGTGCAGGCCCTGGTGGGTATGTGGCGGCGGTTCGTGCCGCCCAACTAGGCAA
AAAAGTAGCCATCATTGAAAAAAGAAAAACACTCGGTGGGACTTGCCTGAACGTAGGTTGTATCCCTTCCAAGGCTCTCC
TCGATTCCTCTGAAGAATTCCATAAAACCAAACATAAGTTAGCGGACCATGGAATCTCTGTGAAAGATGTCAAAATTGAT
ATCGCCAAAATGATGGCACGAAAAGACAAAGTGGTGAGTGAAGTGACATCGGGTGTTGACTACTTGATGAAAAAAAACAA
AATCACTCGTTACTTGGGCCAAGCCAGTTTTGTTTCCAAAACCGAAGTTTCGATCACCGCAGACGATGGCAAAAAAGAAT
CCATTAGCGGAACGAACATCATCATCGCCACAGGTTCTACTCCCATTGAAATCCCACCACTCCCCGTGGATGGAAAAAAC
ATTGTGACCTCTGACCATGCCATTGGGTTTGATTCCGTTCCCGAACACCTCATCATCGTGGGTGCAGGGGTCATCGGTTT
GGAACTAGGCTCTGTTTGGTTACGACTTGGTGCCAAGGTCACCGTGGTGGAACTCATGCCACGGCTTTTCGGAACCGCTG
ACCAAGCGATGGCAAGCCTAGCGGAAAGATTATTAACACAGCAAGGGATTAACTTTCTCTTTGAAACCAAAGTGCACGGT
GCGAAAGTGAAAGGGAAAAAAGTAGAAGTCGAAATCGAAGGCAAAGACGGAAAAAAATCCGTTCTCGAAGGAGACAAGGT
ACTTGTTTCCATTGGCCGACGCCCGAACACAGACGGACTCGGTGCCAAAGAAATTGGAATCGAGATGACAGACCGTGGTC
GCGTCAAAGTAGAACCTAACAAATTCCAAACGAACATTCCGAATATTTATGCGATTGGTGACGTGGTGGACGGACCAATG
CTTGCTCACAAAGCAGAAGACGAAGGGATTGCTGTAGCCGAACTCATTTGTGGTAAGTATGGCCATGTAAATTACAAAGC
CATCCCTTGGATCGTCTACACTTGGCCAGAAGTGGCTTGGGTGGGACTTGGCGAAGAAGAACTAAAAGCCAAAGGGATCG
AATACAAAGTGGGTAAGTACATGTTCAAACCCAATGCAAGAGCCAAAGCCATGAACGAAACCGATGGGCAAGTGAAAGTC
CTCGCGGACAAAAAAACGGACAAACTTCTCGGCGTCTACATTGTCGGCCCTCGTGCATCTGACATGATTGCAGAAGCGGC
GATTGCCTTTGAATTTGGTGCCAGTGCGGAAGACATTGCTCGTTCCACACATGCCCACCCCACTCTTTCCGAAGTCCTTC
GGGAAGCGGCGATGGATGCTGATGCGAAATGGTCCATCCATTCGTAA

Upstream 100 bases:

>100_bases
CACCGCATTGTGGATGGAAAGGAAGCTGTGCAGTTCCTTGTGAAGATCAAAGAAATGGTAGAGGACCCAACGAGACTCCT
CTTTGAGGTATAAGGATTTT

Downstream 100 bases:

>100_bases
CCAAACAGTTGTTTTGTTGTTTTAGGGAGAATATATGACAACCGATCAGATGATGAGTTTATACGGCGATAACGTTGTAT
TATTGGAAGAGTATTACAAA

Product: dihydrolipoyl dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MEQYDIIVIGAGPGGYVAAVRAAQLGKKVAIIEKRKTLGGTCLNVGCIPSKALLDSSEEFHKTKHKLADHGISVKDVKID
IAKMMARKDKVVSEVTSGVDYLMKKNKITRYLGQASFVSKTEVSITADDGKKESISGTNIIIATGSTPIEIPPLPVDGKN
IVTSDHAIGFDSVPEHLIIVGAGVIGLELGSVWLRLGAKVTVVELMPRLFGTADQAMASLAERLLTQQGINFLFETKVHG
AKVKGKKVEVEIEGKDGKKSVLEGDKVLVSIGRRPNTDGLGAKEIGIEMTDRGRVKVEPNKFQTNIPNIYAIGDVVDGPM
LAHKAEDEGIAVAELICGKYGHVNYKAIPWIVYTWPEVAWVGLGEEELKAKGIEYKVGKYMFKPNARAKAMNETDGQVKV
LADKKTDKLLGVYIVGPRASDMIAEAAIAFEFGASAEDIARSTHAHPTLSEVLREAAMDADAKWSIHS

Sequences:

>Translated_468_residues
MEQYDIIVIGAGPGGYVAAVRAAQLGKKVAIIEKRKTLGGTCLNVGCIPSKALLDSSEEFHKTKHKLADHGISVKDVKID
IAKMMARKDKVVSEVTSGVDYLMKKNKITRYLGQASFVSKTEVSITADDGKKESISGTNIIIATGSTPIEIPPLPVDGKN
IVTSDHAIGFDSVPEHLIIVGAGVIGLELGSVWLRLGAKVTVVELMPRLFGTADQAMASLAERLLTQQGINFLFETKVHG
AKVKGKKVEVEIEGKDGKKSVLEGDKVLVSIGRRPNTDGLGAKEIGIEMTDRGRVKVEPNKFQTNIPNIYAIGDVVDGPM
LAHKAEDEGIAVAELICGKYGHVNYKAIPWIVYTWPEVAWVGLGEEELKAKGIEYKVGKYMFKPNARAKAMNETDGQVKV
LADKKTDKLLGVYIVGPRASDMIAEAAIAFEFGASAEDIARSTHAHPTLSEVLREAAMDADAKWSIHS
>Mature_468_residues
MEQYDIIVIGAGPGGYVAAVRAAQLGKKVAIIEKRKTLGGTCLNVGCIPSKALLDSSEEFHKTKHKLADHGISVKDVKID
IAKMMARKDKVVSEVTSGVDYLMKKNKITRYLGQASFVSKTEVSITADDGKKESISGTNIIIATGSTPIEIPPLPVDGKN
IVTSDHAIGFDSVPEHLIIVGAGVIGLELGSVWLRLGAKVTVVELMPRLFGTADQAMASLAERLLTQQGINFLFETKVHG
AKVKGKKVEVEIEGKDGKKSVLEGDKVLVSIGRRPNTDGLGAKEIGIEMTDRGRVKVEPNKFQTNIPNIYAIGDVVDGPM
LAHKAEDEGIAVAELICGKYGHVNYKAIPWIVYTWPEVAWVGLGEEELKAKGIEYKVGKYMFKPNARAKAMNETDGQVKV
LADKKTDKLLGVYIVGPRASDMIAEAAIAFEFGASAEDIARSTHAHPTLSEVLREAAMDADAKWSIHS

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=467, Percent_Identity=51.3918629550321, Blast_Score=459, Evalue=1e-129,
Organism=Homo sapiens, GI50301238, Length=465, Percent_Identity=30.752688172043, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI22035672, Length=456, Percent_Identity=30.4824561403509, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI148277065, Length=477, Percent_Identity=26.8343815513627, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI33519430, Length=477, Percent_Identity=26.8343815513627, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI33519428, Length=477, Percent_Identity=26.8343815513627, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI33519426, Length=477, Percent_Identity=26.8343815513627, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI148277071, Length=477, Percent_Identity=26.8343815513627, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI291045266, Length=486, Percent_Identity=27.5720164609054, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI291045268, Length=480, Percent_Identity=26.25, Blast_Score=107, Evalue=3e-23,
Organism=Escherichia coli, GI1786307, Length=452, Percent_Identity=41.1504424778761, Blast_Score=335, Evalue=4e-93,
Organism=Escherichia coli, GI87082354, Length=463, Percent_Identity=32.3974082073434, Blast_Score=225, Evalue=6e-60,
Organism=Escherichia coli, GI87081717, Length=468, Percent_Identity=27.5641025641026, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1789915, Length=436, Percent_Identity=27.0642201834862, Blast_Score=147, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI32565766, Length=466, Percent_Identity=51.7167381974249, Blast_Score=478, Evalue=1e-135,
Organism=Caenorhabditis elegans, GI17557007, Length=471, Percent_Identity=30.3609341825902, Blast_Score=149, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI71982272, Length=484, Percent_Identity=28.099173553719, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI71983429, Length=466, Percent_Identity=27.0386266094421, Blast_Score=107, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI71983419, Length=466, Percent_Identity=27.0386266094421, Blast_Score=107, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6321091, Length=477, Percent_Identity=48.0083857442348, Blast_Score=432, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6325240, Length=476, Percent_Identity=33.8235294117647, Blast_Score=254, Evalue=2e-68,
Organism=Saccharomyces cerevisiae, GI6325166, Length=464, Percent_Identity=26.2931034482759, Blast_Score=150, Evalue=6e-37,
Organism=Drosophila melanogaster, GI21358499, Length=461, Percent_Identity=51.409978308026, Blast_Score=468, Evalue=1e-132,
Organism=Drosophila melanogaster, GI24640549, Length=486, Percent_Identity=29.8353909465021, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24640553, Length=486, Percent_Identity=29.8353909465021, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24640551, Length=486, Percent_Identity=29.8353909465021, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI17737741, Length=480, Percent_Identity=26.875, Blast_Score=129, Evalue=5e-30,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50408; Mature: 50408

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQYDIIVIGAGPGGYVAAVRAAQLGKKVAIIEKRKTLGGTCLNVGCIPSKALLDSSEEF
CCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCCCEEEECCCCCHHHHCCCHHH
HKTKHKLADHGISVKDVKIDIAKMMARKDKVVSEVTSGVDYLMKKNKITRYLGQASFVSK
HHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
TEVSITADDGKKESISGTNIIIATGSTPIEIPPLPVDGKNIVTSDHAIGFDSVPEHLIIV
EEEEEECCCCCCCCCCCCEEEEEECCCCEECCCCCCCCCCEEECCCCCCCCCCCCEEEEE
GAGVIGLELGSVWLRLGAKVTVVELMPRLFGTADQAMASLAERLLTQQGINFLFETKVHG
ECCHHHHHHHHHHHHHCCCEEHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEECCCC
AKVKGKKVEVEIEGKDGKKSVLEGDKVLVSIGRRPNTDGLGAKEIGIEMTDRGRVKVEPN
CEECCCEEEEEEECCCCCHHHHCCCEEEEEECCCCCCCCCCCHHCCEEECCCCEEEECCC
KFQTNIPNIYAIGDVVDGPMLAHKAEDEGIAVAELICGKYGHVNYKAIPWIVYTWPEVAW
HHCCCCCCEEEECCCCCCCCEEECCCCCCCCHHHHHHCCCCCCCEEEECEEEEECCCEEE
VGLGEEELKAKGIEYKVGKYMFKPNARAKAMNETDGQVKVLADKKTDKLLGVYIVGPRAS
ECCCHHHHHHCCCEEECCEEEECCCCCCCCCCCCCCEEEEEECCCCCCEEEEEEECCCCH
DMIAEAAIAFEFGASAEDIARSTHAHPTLSEVLREAAMDADAKWSIHS
HHHHHHHHEEECCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCEECCC
>Mature Secondary Structure
MEQYDIIVIGAGPGGYVAAVRAAQLGKKVAIIEKRKTLGGTCLNVGCIPSKALLDSSEEF
CCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCCCEEEECCCCCHHHHCCCHHH
HKTKHKLADHGISVKDVKIDIAKMMARKDKVVSEVTSGVDYLMKKNKITRYLGQASFVSK
HHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
TEVSITADDGKKESISGTNIIIATGSTPIEIPPLPVDGKNIVTSDHAIGFDSVPEHLIIV
EEEEEECCCCCCCCCCCCEEEEEECCCCEECCCCCCCCCCEEECCCCCCCCCCCCEEEEE
GAGVIGLELGSVWLRLGAKVTVVELMPRLFGTADQAMASLAERLLTQQGINFLFETKVHG
ECCHHHHHHHHHHHHHCCCEEHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEECCCC
AKVKGKKVEVEIEGKDGKKSVLEGDKVLVSIGRRPNTDGLGAKEIGIEMTDRGRVKVEPN
CEECCCEEEEEEECCCCCHHHHCCCEEEEEECCCCCCCCCCCHHCCEEECCCCEEEECCC
KFQTNIPNIYAIGDVVDGPMLAHKAEDEGIAVAELICGKYGHVNYKAIPWIVYTWPEVAW
HHCCCCCCEEEECCCCCCCCEEECCCCCCCCHHHHHHCCCCCCCEEEECEEEEECCCEEE
VGLGEEELKAKGIEYKVGKYMFKPNARAKAMNETDGQVKVLADKKTDKLLGVYIVGPRAS
ECCCHHHHHHCCCEEECCEEEECCCCCCCCCCCCCCEEEEEECCCCCCEEEEEEECCCCH
DMIAEAAIAFEFGASAEDIARSTHAHPTLSEVLREAAMDADAKWSIHS
HHHHHHHHEEECCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867378 [H]