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The map label for this gene is sucB [H]

Identifier: 183220461

GI number: 183220461

Start: 1099793

End: 1101025

Strand: Reverse

Name: sucB [H]

Synonym: LEPBI_I1058

Alternate gene names: 183220461

Gene position: 1101025-1099793 (Counterclockwise)

Preceding gene: 183220463

Following gene: 183220460

Centisome position: 30.59

GC content: 47.69

Gene sequence:

>1233_bases
ATGGCAATAGAAATCAAAGTCCCCGAGATGGGGGAATCCGTAACCGAAGCGACCATCAGTGCTTGGACCAAAAAAGAAGG
CGATGCCGTAAAAGTAGACGAAGTGCTCGCTATTTTAGAAACAGACAAAGTTTCATTAGAAATCCCTGCCCCGAGTTCCG
GGGTTTTAAAATCCATTACCAAAAAGGTGGGGGATGTGGTGCATGTCCGAGACATCATGGGCATGATCGAAGAAGGGGCG
GTCGCGGCAGCTCCTGTGAGTTCTGGAGGAGCGGCTCCTAAAGTTGAAACTCCAAGCGCACAACCTAACACGGGCAAAGT
GAATGACGAACTCCCTCCTGCGGCGCGTAAACTCATCGAAGAAAATAAATTAGATGCCACAAAAATCACAGGCACGGGTC
GAAACGGCCAAATCACAAAAGAAGATGTGATCCTTTTTATGGAAAAAGGTGGGGCAGGTTCTGTTGCTCCTTCCAAAACT
TCTGCACCAAGTCCTGAGATTCCAAAAGCAGTAGTGGTTAGTGCAAACTCTGGACCAAGAGAAACAGTTGTACCAATGAC
AAAACTCCGCCAAACGATCGCTAACCGATTGGTGAATGCACAACACACAGCGGCCATCCTCACGACATTCAACGAAGTAG
ATATGTCACCGATCATGGAACTTCGCAATAAATACAAAGACAAGTTCAAAGAAACTCATGGTGTGGGTCTTGGTTTCATG
TCTCTTTTCACAAAAGCAGCGGTGGCAGCCCTTAAGGCTTTCCCTGCGATCAATGCGGAAATTCGCGGAACAGACATTGT
CTACAAAAACTACTACGACATCGGAGTGGCAGTGGGTGGACCGAAAGGACTTGTGGTTCCGATTGTTCGTAACGCCGACT
TACTGAGCTTTGCTGGTGTGGAACAAGAGATCGCAAGGCTTGCGGGCAAAGTGAAAGACGGAAAAATTTCTTTGGAAGAC
ATGGAAGGGGGAACCTTCTCTATCTCGAATGGTGGTGTGTATGGATCGATGATGTCGACACCGATACTCAACCCTCCTCA
ATCAGGAATCCTTGGGATGCACAACATCGTCAAACGCGCCGTCGTTGTGAACGATCAAATTGTGATCCGTCCGATGATGT
ATCTCGCACTTTCCTATGACCACCGCATTGTGGATGGAAAGGAAGCTGTGCAGTTCCTTGTGAAGATCAAAGAAATGGTA
GAGGACCCAACGAGACTCCTCTTTGAGGTATAA

Upstream 100 bases:

>100_bases
CGTTTTAACCTTTAAGTAAGTTACCGTAGTCTTAGATTCAAATGGGATCTCACTCCTCAAAAACGCTATTTTTCTCTAAA
AACCTGCTAAGGAATATTTC

Downstream 100 bases:

>100_bases
GGATTTTATGGAACAATACGATATCATTGTGATTGGTGCAGGCCCTGGTGGGTATGTGGCGGCGGTTCGTGCCGCCCAAC
TAGGCAAAAAAGTAGCCATC

Product: 2-oxoglutarate dehydrogenase complex succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MAIEIKVPEMGESVTEATISAWTKKEGDAVKVDEVLAILETDKVSLEIPAPSSGVLKSITKKVGDVVHVRDIMGMIEEGA
VAAAPVSSGGAAPKVETPSAQPNTGKVNDELPPAARKLIEENKLDATKITGTGRNGQITKEDVILFMEKGGAGSVAPSKT
SAPSPEIPKAVVVSANSGPRETVVPMTKLRQTIANRLVNAQHTAAILTTFNEVDMSPIMELRNKYKDKFKETHGVGLGFM
SLFTKAAVAALKAFPAINAEIRGTDIVYKNYYDIGVAVGGPKGLVVPIVRNADLLSFAGVEQEIARLAGKVKDGKISLED
MEGGTFSISNGGVYGSMMSTPILNPPQSGILGMHNIVKRAVVVNDQIVIRPMMYLALSYDHRIVDGKEAVQFLVKIKEMV
EDPTRLLFEV

Sequences:

>Translated_410_residues
MAIEIKVPEMGESVTEATISAWTKKEGDAVKVDEVLAILETDKVSLEIPAPSSGVLKSITKKVGDVVHVRDIMGMIEEGA
VAAAPVSSGGAAPKVETPSAQPNTGKVNDELPPAARKLIEENKLDATKITGTGRNGQITKEDVILFMEKGGAGSVAPSKT
SAPSPEIPKAVVVSANSGPRETVVPMTKLRQTIANRLVNAQHTAAILTTFNEVDMSPIMELRNKYKDKFKETHGVGLGFM
SLFTKAAVAALKAFPAINAEIRGTDIVYKNYYDIGVAVGGPKGLVVPIVRNADLLSFAGVEQEIARLAGKVKDGKISLED
MEGGTFSISNGGVYGSMMSTPILNPPQSGILGMHNIVKRAVVVNDQIVIRPMMYLALSYDHRIVDGKEAVQFLVKIKEMV
EDPTRLLFEV
>Mature_409_residues
AIEIKVPEMGESVTEATISAWTKKEGDAVKVDEVLAILETDKVSLEIPAPSSGVLKSITKKVGDVVHVRDIMGMIEEGAV
AAAPVSSGGAAPKVETPSAQPNTGKVNDELPPAARKLIEENKLDATKITGTGRNGQITKEDVILFMEKGGAGSVAPSKTS
APSPEIPKAVVVSANSGPRETVVPMTKLRQTIANRLVNAQHTAAILTTFNEVDMSPIMELRNKYKDKFKETHGVGLGFMS
LFTKAAVAALKAFPAINAEIRGTDIVYKNYYDIGVAVGGPKGLVVPIVRNADLLSFAGVEQEIARLAGKVKDGKISLEDM
EGGTFSISNGGVYGSMMSTPILNPPQSGILGMHNIVKRAVVVNDQIVIRPMMYLALSYDHRIVDGKEAVQFLVKIKEMVE
DPTRLLFEV

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and l

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=227, Percent_Identity=53.3039647577093, Blast_Score=265, Evalue=5e-71,
Organism=Homo sapiens, GI110671329, Length=436, Percent_Identity=27.0642201834862, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI203098753, Length=452, Percent_Identity=27.8761061946903, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI203098816, Length=452, Percent_Identity=27.8761061946903, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI31711992, Length=440, Percent_Identity=26.1363636363636, Blast_Score=143, Evalue=3e-34,
Organism=Homo sapiens, GI260898739, Length=212, Percent_Identity=31.6037735849057, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI1786946, Length=412, Percent_Identity=48.5436893203884, Blast_Score=394, Evalue=1e-111,
Organism=Escherichia coli, GI1786305, Length=426, Percent_Identity=29.3427230046948, Blast_Score=148, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI25146366, Length=415, Percent_Identity=42.1686746987952, Blast_Score=298, Evalue=4e-81,
Organism=Caenorhabditis elegans, GI17537937, Length=423, Percent_Identity=26.9503546099291, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17560088, Length=446, Percent_Identity=28.6995515695067, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17538894, Length=308, Percent_Identity=31.1688311688312, Blast_Score=128, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6320352, Length=410, Percent_Identity=45.609756097561, Blast_Score=336, Evalue=4e-93,
Organism=Saccharomyces cerevisiae, GI6324258, Length=452, Percent_Identity=26.5486725663717, Blast_Score=135, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24645909, Length=223, Percent_Identity=56.0538116591928, Blast_Score=268, Evalue=6e-72,
Organism=Drosophila melanogaster, GI18859875, Length=431, Percent_Identity=27.3781902552204, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI20129315, Length=229, Percent_Identity=31.4410480349345, Blast_Score=114, Evalue=1e-25,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 43729; Mature: 43598

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIEIKVPEMGESVTEATISAWTKKEGDAVKVDEVLAILETDKVSLEIPAPSSGVLKSIT
CEEEEECCCCCCHHHHHHHHHHHCCCCCEEEHHHHHHHEECCCEEEEECCCCHHHHHHHH
KKVGDVVHVRDIMGMIEEGAVAAAPVSSGGAAPKVETPSAQPNTGKVNDELPPAARKLIE
HHHCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
ENKLDATKITGTGRNGQITKEDVILFMEKGGAGSVAPSKTSAPSPEIPKAVVVSANSGPR
HCCCCCEEEECCCCCCCEEHHHEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCC
ETVVPMTKLRQTIANRLVNAQHTAAILTTFNEVDMSPIMELRNKYKDKFKETHGVGLGFM
CCCCCHHHHHHHHHHHHCCCHHHHHEEHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHH
SLFTKAAVAALKAFPAINAEIRGTDIVYKNYYDIGVAVGGPKGLVVPIVRNADLLSFAGV
HHHHHHHHHHHHHCCCCCCEECCCEEEEECEEEEEEEECCCCCEEEEEECCCCHHHHCCH
EQEIARLAGKVKDGKISLEDMEGGTFSISNGGVYGSMMSTPILNPPQSGILGMHNIVKRA
HHHHHHHHCCCCCCEEEEEECCCCEEEECCCCEECHHHCCCCCCCCCCCCHHHHHHHHHH
VVVNDQIVIRPMMYLALSYDHRIVDGKEAVQFLVKIKEMVEDPTRLLFEV
HHCCCCHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHCCHHHHHCCC
>Mature Secondary Structure 
AIEIKVPEMGESVTEATISAWTKKEGDAVKVDEVLAILETDKVSLEIPAPSSGVLKSIT
EEEEECCCCCCHHHHHHHHHHHCCCCCEEEHHHHHHHEECCCEEEEECCCCHHHHHHHH
KKVGDVVHVRDIMGMIEEGAVAAAPVSSGGAAPKVETPSAQPNTGKVNDELPPAARKLIE
HHHCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
ENKLDATKITGTGRNGQITKEDVILFMEKGGAGSVAPSKTSAPSPEIPKAVVVSANSGPR
HCCCCCEEEECCCCCCCEEHHHEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCC
ETVVPMTKLRQTIANRLVNAQHTAAILTTFNEVDMSPIMELRNKYKDKFKETHGVGLGFM
CCCCCHHHHHHHHHHHHCCCHHHHHEEHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHH
SLFTKAAVAALKAFPAINAEIRGTDIVYKNYYDIGVAVGGPKGLVVPIVRNADLLSFAGV
HHHHHHHHHHHHHCCCCCCEECCCEEEEECEEEEEEEECCCCCEEEEEECCCCHHHHCCH
EQEIARLAGKVKDGKISLEDMEGGTFSISNGGVYGSMMSTPILNPPQSGILGMHNIVKRA
HHHHHHHHCCCCCCEEEEEECCCCEEEECCCCEECHHHCCCCCCCCCCCCHHHHHHHHHH
VVVNDQIVIRPMMYLALSYDHRIVDGKEAVQFLVKIKEMVEDPTRLLFEV
HHCCCCHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12874367 [H]