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The map label for this gene is dapA

Identifier: 183220145

GI number: 183220145

Start: 747953

End: 748837

Strand: Direct

Name: dapA

Synonym: LEPBI_I0733

Alternate gene names: 183220145

Gene position: 747953-748837 (Clockwise)

Preceding gene: 183220142

Following gene: 183220146

Centisome position: 20.78

GC content: 43.73

Gene sequence:

>885_bases
ATGTTTCAGGGCGTTTATACTGCGGTCATCACCCCCTTCCGCCAGGGGAAAATCGATTATGATAGTTATTTTAAGATCCT
AGAAAACCAGATCCGTTCCGGTGTCGCTGGTGTGGTCCCTTGTGGGACAACGGGAGAGTCTCCGACCTTATCCTACGAAG
AACACAAGGAACTCATCCAAAAAACCGTGCAAGTGGTTTCAGGGAAGATCCAAGTGATCGCAGGCACAGGTTCCAATTCT
ACCAAAGAAGCAATTGAGCTCACAGAGTCAGCTTGTGCGGACGGAGTGGACGGAATCCTTTCCGTTAACCCATATTACAA
CAAACCTACCCAAGAAGGGATGTTCCAACATTTTACAGCGATTGCCAATGTTTCATCCAAACCGGTCATGTTGTACAATA
TCCCTGGTAGAACCAATGTTAATTTATTACCAGAAACTGTCAGTCGTTTGGCGGCGCATCCAAAAATTGCGGCGATCAAA
GAAGCAACAGGTGACTTGGGACAAATGGCAAAGGTAATTTCCCAATGCCCTACCAACTTTGATTTATTATCTGGTGATGA
CAACCTAACACTGCCAGTACTCTCGATCGGTGGAAAAGGGGTTGTATCCGTTGTTTCTAATTTATTCCCACGCGCTTGTG
TGGATATGGTGTCCTTGTACTTACGCGGAGACTTAGAAGCTTCCAAAAAGATATATTACAAACTGCTTCCTGTTTTTGTA
AATGCCTTCATTGAAACAAACCCAATCCCTATCAAAGCAGCGATGAGTTGGTTTGGGTATTGTAGTAACGAACTCAGATT
ACCGATGACTTCTTTATCTGAAGGTACAGCATCAGAGTCGTTCAAAAAAATTGTATTCCAACTAAAAGAGGAAGGCATTG
TCTAA

Upstream 100 bases:

>100_bases
GAAACAGGGAAAAAAGGGACCATTTTCGCAAAGTTTCTCAGATCAGGGTGTTTTGGCAGTTTTTTATTGCAAAAAACAGT
CCCTCCTCGATGTTTAATTT

Downstream 100 bases:

>100_bases
AATCAAAGTAGGTGTGATAGGGGCTGGAGGAAGGATGGGGAAAGCCATCATCCAAGTACTTTCCCTTTCCAAAAAATCAG
AGTTAAGCGCTGCTGTTGTG

Product: dihydrodipicolinate synthase

Products: NA

Alternate protein names: DHDPS

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MFQGVYTAVITPFRQGKIDYDSYFKILENQIRSGVAGVVPCGTTGESPTLSYEEHKELIQKTVQVVSGKIQVIAGTGSNS
TKEAIELTESACADGVDGILSVNPYYNKPTQEGMFQHFTAIANVSSKPVMLYNIPGRTNVNLLPETVSRLAAHPKIAAIK
EATGDLGQMAKVISQCPTNFDLLSGDDNLTLPVLSIGGKGVVSVVSNLFPRACVDMVSLYLRGDLEASKKIYYKLLPVFV
NAFIETNPIPIKAAMSWFGYCSNELRLPMTSLSEGTASESFKKIVFQLKEEGIV

Sequences:

>Translated_294_residues
MFQGVYTAVITPFRQGKIDYDSYFKILENQIRSGVAGVVPCGTTGESPTLSYEEHKELIQKTVQVVSGKIQVIAGTGSNS
TKEAIELTESACADGVDGILSVNPYYNKPTQEGMFQHFTAIANVSSKPVMLYNIPGRTNVNLLPETVSRLAAHPKIAAIK
EATGDLGQMAKVISQCPTNFDLLSGDDNLTLPVLSIGGKGVVSVVSNLFPRACVDMVSLYLRGDLEASKKIYYKLLPVFV
NAFIETNPIPIKAAMSWFGYCSNELRLPMTSLSEGTASESFKKIVFQLKEEGIV
>Mature_294_residues
MFQGVYTAVITPFRQGKIDYDSYFKILENQIRSGVAGVVPCGTTGESPTLSYEEHKELIQKTVQVVSGKIQVIAGTGSNS
TKEAIELTESACADGVDGILSVNPYYNKPTQEGMFQHFTAIANVSSKPVMLYNIPGRTNVNLLPETVSRLAAHPKIAAIK
EATGDLGQMAKVISQCPTNFDLLSGDDNLTLPVLSIGGKGVVSVVSNLFPRACVDMVSLYLRGDLEASKKIYYKLLPVFV
NAFIETNPIPIKAAMSWFGYCSNELRLPMTSLSEGTASESFKKIVFQLKEEGIV

Specific function: Biosynthesis of diaminopimelate and lysine from aspartate semialdehyde; first step. [C]

COG id: COG0329

COG function: function code EM; Dihydrodipicolinate synthase/N-acetylneuraminate lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHDPS family

Homologues:

Organism=Homo sapiens, GI31543060, Length=279, Percent_Identity=28.673835125448, Blast_Score=121, Evalue=7e-28,
Organism=Homo sapiens, GI13540533, Length=232, Percent_Identity=27.5862068965517, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1788823, Length=276, Percent_Identity=42.3913043478261, Blast_Score=232, Evalue=3e-62,
Organism=Escherichia coli, GI87082415, Length=240, Percent_Identity=32.0833333333333, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1786463, Length=243, Percent_Identity=30.0411522633745, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1789620, Length=232, Percent_Identity=28.8793103448276, Blast_Score=105, Evalue=2e-24,

Paralogues:

None

Copy number: 840 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): DAPA_LEPBA (B0SCT0)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001961820.1
- ProteinModelPortal:   B0SCT0
- SMR:   B0SCT0
- GeneID:   6388338
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_0710
- HOGENOM:   HBG358848
- OMA:   QKGLIEH
- ProtClustDB:   PRK03170
- BioCyc:   LBIF355278:LBF_0710-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00418
- InterPro:   IPR013785
- InterPro:   IPR005263
- InterPro:   IPR002220
- InterPro:   IPR020625
- InterPro:   IPR020624
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR12128
- PRINTS:   PR00146
- TIGRFAMs:   TIGR00674

Pfam domain/function: PF00701 DHDPS

EC number: =4.2.1.52

Molecular weight: Translated: 31889; Mature: 31889

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00665 DHDPS_1; PS00666 DHDPS_2

Important sites: ACT_SITE 160-160 BINDING 105-105

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQGVYTAVITPFRQGKIDYDSYFKILENQIRSGVAGVVPCGTTGESPTLSYEEHKELIQ
CCCCHHHHHHCHHHCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHH
KTVQVVSGKIQVIAGTGSNSTKEAIELTESACADGVDGILSVNPYYNKPTQEGMFQHFTA
HHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHH
IANVSSKPVMLYNIPGRTNVNLLPETVSRLAAHPKIAAIKEATGDLGQMAKVISQCPTNF
HHCCCCCCEEEEECCCCCCCCCCHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHCCCCC
DLLSGDDNLTLPVLSIGGKGVVSVVSNLFPRACVDMVSLYLRGDLEASKKIYYKLLPVFV
EEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
NAFIETNPIPIKAAMSWFGYCSNELRLPMTSLSEGTASESFKKIVFQLKEEGIV
HHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFQGVYTAVITPFRQGKIDYDSYFKILENQIRSGVAGVVPCGTTGESPTLSYEEHKELIQ
CCCCHHHHHHCHHHCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHH
KTVQVVSGKIQVIAGTGSNSTKEAIELTESACADGVDGILSVNPYYNKPTQEGMFQHFTA
HHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHH
IANVSSKPVMLYNIPGRTNVNLLPETVSRLAAHPKIAAIKEATGDLGQMAKVISQCPTNF
HHCCCCCCEEEEECCCCCCCCCCHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHCCCCC
DLLSGDDNLTLPVLSIGGKGVVSVVSNLFPRACVDMVSLYLRGDLEASKKIYYKLLPVFV
EEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
NAFIETNPIPIKAAMSWFGYCSNELRLPMTSLSEGTASESFKKIVFQLKEEGIV
HHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA