Click here to switch to the map view.

The map label for this gene is ispA2 [H]

Identifier: 183220059

GI number: 183220059

Start: 650505

End: 651458

Strand: Direct

Name: ispA2 [H]

Synonym: LEPBI_I0646

Alternate gene names: 183220059

Gene position: 650505-651458 (Clockwise)

Preceding gene: 183220058

Following gene: 183220062

Centisome position: 18.07

GC content: 35.43

Gene sequence:

>954_bases
ATGAAGTCAAAGATTAAGATCCAATCTGTACTGTCCAAATTTGATAAAAATTTAGACAGTATCATTCACGAAGACATTCC
CATCCTTAAAAAAATAAAAAAACAAGTCATCACATCCGGAGGAAAAAGAATTCGACCTTTCTCCCATTATCTCTTTTGTC
AATTTATGAATGTAAAGGATGTTAGTTGGTTAGATGTTGGAAGTGTTGCCGAGCTGATCCATGCGGCAAGTTTGTTACAT
GATGATGTGGTTGACAATGCCCCCATTCGTCGTGGAAAACCAACCATTGGTGCTAGTTTCGGTAACAAAACGGCAATCCT
TGCTGGAGATTATCTATTGGCTTGTGGGATTAGCCGACTGAACTCACTTGGCAATCCTGATCTTATGGAAATTTTTTCCC
AAGTGTTAAGAGATCTTTCTGTGAGTGAACTTTTGCAGATGGAATGGGAAAAAAATCCAAATATCACATTAAAAATCTAC
GATCAAATCATTTATGGAAAAACGGCTTCCTTATTCGGTGTTTGTACGGAAGCAGCAGCTATATTGGCAAACCAATCAAA
GGAAAAACGAAAAGAGTTTCGTCAATTCGGTGTTAGGTTAGGGAAGTTATTCCAAAAAAAAGATGATTGTTTGGATTATT
TTGAAGATTCCAAGGCAAGTGGAAAGGAATTTTTAAAAGATTTTAAAAACGGTCTTTTTACCTATCCTGTTTTGGTATTA
CGCTCAAAACTAAACCTTTTGGAAAAACGGAAGTTAAACCGATTGTTCCAAAAAGAAATTCGTGATGTAAAAGATGAATC
TATAATTTTGGATTTAATGAATTCCAAAAAAATTCCAGCCATCCTGCACAAAGAATTGGAATCCGAAAAAAATGATTTAT
TACGATTCCTCAATCAGTTTCCTAAATCAGAGGAACGAGAACTCTTTATAGAACAACTGAGTCGTCTTACTTGA

Upstream 100 bases:

>100_bases
ACTCCATATCTATCGCGACAATCAGTCCAAAGAAATGGAACATATGATTAAGGATCATCTTCAGATTCTCGAAAGTCGAT
CCACTCATGTGGAATAACGG

Downstream 100 bases:

>100_bases
GTTTGGTTTTTTAAGAAAAATGATTCATTCCGTGGCGTGAGGGTCGATGAGTTCGATCGCAGGTAATAGAATACAAAAAC
AAGTCCTTCCTGGTTCTGAT

Product: octoprenyl-diphosphate synthase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MKSKIKIQSVLSKFDKNLDSIIHEDIPILKKIKKQVITSGGKRIRPFSHYLFCQFMNVKDVSWLDVGSVAELIHAASLLH
DDVVDNAPIRRGKPTIGASFGNKTAILAGDYLLACGISRLNSLGNPDLMEIFSQVLRDLSVSELLQMEWEKNPNITLKIY
DQIIYGKTASLFGVCTEAAAILANQSKEKRKEFRQFGVRLGKLFQKKDDCLDYFEDSKASGKEFLKDFKNGLFTYPVLVL
RSKLNLLEKRKLNRLFQKEIRDVKDESIILDLMNSKKIPAILHKELESEKNDLLRFLNQFPKSEERELFIEQLSRLT

Sequences:

>Translated_317_residues
MKSKIKIQSVLSKFDKNLDSIIHEDIPILKKIKKQVITSGGKRIRPFSHYLFCQFMNVKDVSWLDVGSVAELIHAASLLH
DDVVDNAPIRRGKPTIGASFGNKTAILAGDYLLACGISRLNSLGNPDLMEIFSQVLRDLSVSELLQMEWEKNPNITLKIY
DQIIYGKTASLFGVCTEAAAILANQSKEKRKEFRQFGVRLGKLFQKKDDCLDYFEDSKASGKEFLKDFKNGLFTYPVLVL
RSKLNLLEKRKLNRLFQKEIRDVKDESIILDLMNSKKIPAILHKELESEKNDLLRFLNQFPKSEERELFIEQLSRLT
>Mature_317_residues
MKSKIKIQSVLSKFDKNLDSIIHEDIPILKKIKKQVITSGGKRIRPFSHYLFCQFMNVKDVSWLDVGSVAELIHAASLLH
DDVVDNAPIRRGKPTIGASFGNKTAILAGDYLLACGISRLNSLGNPDLMEIFSQVLRDLSVSELLQMEWEKNPNITLKIY
DQIIYGKTASLFGVCTEAAAILANQSKEKRKEFRQFGVRLGKLFQKKDDCLDYFEDSKASGKEFLKDFKNGLFTYPVLVL
RSKLNLLEKRKLNRLFQKEIRDVKDESIILDLMNSKKIPAILHKELESEKNDLLRFLNQFPKSEERELFIEQLSRLT

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=217, Percent_Identity=35.0230414746544, Blast_Score=118, Evalue=7e-27,
Organism=Homo sapiens, GI4758430, Length=212, Percent_Identity=25.9433962264151, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI83700220, Length=212, Percent_Identity=25.9433962264151, Blast_Score=89, Evalue=7e-18,
Organism=Escherichia coli, GI1789578, Length=217, Percent_Identity=34.5622119815668, Blast_Score=151, Evalue=6e-38,
Organism=Escherichia coli, GI1786623, Length=245, Percent_Identity=28.9795918367347, Blast_Score=73, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17505681, Length=195, Percent_Identity=37.9487179487179, Blast_Score=133, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6319475, Length=220, Percent_Identity=33.6363636363636, Blast_Score=106, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6325188, Length=219, Percent_Identity=26.4840182648402, Blast_Score=73, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24651612, Length=220, Percent_Identity=32.2727272727273, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI281365769, Length=199, Percent_Identity=22.6130653266332, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24660002, Length=199, Percent_Identity=22.6130653266332, Blast_Score=76, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 36341; Mature: 36341

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSKIKIQSVLSKFDKNLDSIIHEDIPILKKIKKQVITSGGKRIRPFSHYLFCQFMNVKD
CCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCC
VSWLDVGSVAELIHAASLLHDDVVDNAPIRRGKPTIGASFGNKTAILAGDYLLACGISRL
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHH
NSLGNPDLMEIFSQVLRDLSVSELLQMEWEKNPNITLKIYDQIIYGKTASLFGVCTEAAA
HCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEHHHHHHCCHHHHHHHHHHHHH
ILANQSKEKRKEFRQFGVRLGKLFQKKDDCLDYFEDSKASGKEFLKDFKNGLFTYPVLVL
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHH
RSKLNLLEKRKLNRLFQKEIRDVKDESIILDLMNSKKIPAILHKELESEKNDLLRFLNQF
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHC
PKSEERELFIEQLSRLT
CCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKSKIKIQSVLSKFDKNLDSIIHEDIPILKKIKKQVITSGGKRIRPFSHYLFCQFMNVKD
CCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCC
VSWLDVGSVAELIHAASLLHDDVVDNAPIRRGKPTIGASFGNKTAILAGDYLLACGISRL
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHH
NSLGNPDLMEIFSQVLRDLSVSELLQMEWEKNPNITLKIYDQIIYGKTASLFGVCTEAAA
HCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEHHHHHHCCHHHHHHHHHHHHH
ILANQSKEKRKEFRQFGVRLGKLFQKKDDCLDYFEDSKASGKEFLKDFKNGLFTYPVLVL
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHH
RSKLNLLEKRKLNRLFQKEIRDVKDESIILDLMNSKKIPAILHKELESEKNDLLRFLNQF
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHC
PKSEERELFIEQLSRLT
CCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]