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The map label for this gene is rfbA

Identifier: 183219936

GI number: 183219936

Start: 517165

End: 518196

Strand: Direct

Name: rfbA

Synonym: LEPBI_I0518

Alternate gene names: NA

Gene position: 517165-518196 (Clockwise)

Preceding gene: 183219935

Following gene: 183219937

Centisome position: 14.37

GC content: 35.95

Gene sequence:

>1032_bases
GTGAATCTCTTATTCGACGATTCTAAACGTAATCCCTCTCTTGAACCACTTTCGAGGTTCCATTCATTTTTTGAATGGAA
CTTAGGAGGAATGAATCTACTCGAAAAATTAGATAGAAAATACCCAGGTTCAAAATTTTTTTATAAAGGACCAAATCCGG
ATTTTGAAACATTAATCTTCAATCGTTACCCACATGTTTTTCCAGCAACTTTAGAAAACTATGATTCTATTTATAGTTCT
AATTCCTTTTTACCTTGGGAGTTACTTGGAACTGTAACATCGATTATAGAAGACACTTTGAACATTGATAAAGAATGGAA
ACGGTTTCGCCAAAAGTACAAATCAAAACAATCAGGTTTTCATATTGTTGGCAAAGACAAACACCTTTACATCCATTCAG
GGGCAACCATTTATCCTGGAGTTGTTTTTGATACTACTCATGGTCCCATTCTCATTGAAGATGGAGTCAAAATTTCATCT
TTTAGTTTTTTGGAAGGACCTTTATTTATAGGTAAAAATTGCCAGATCGACAATGCAAGGATCACTGGTGGAACGTTGAT
TGGAAACCAATGTCGGATAGGTGGTGAAGTTGAAAATTCCATCATTTTGGATTATACCAACAAACACCATGAAGGTTTTT
TGGGTCATAGTTTTGTTTCTAGTTGGGTCAATTTAGGTGCTCTGTCCACAACAAGTGATCTAAAAAATAATTATGGAATC
GTGAAACTCAAAGTAGGTGATGCCATCGTCAATACAGGCACCATTAAATTTGGGTCCATCATTGGTCCATTTACAAAACT
TGCGATTGGTGTGATGTCAAACACAGGAACCGTATTTGATATTGCAAGTAACATAGTGGAATCAAGGATCCAAGGGTATG
TGCCTGCATTTACTTGGATCAAACCTGGAGAACGTTACCGACTGGAAGAATTTTTATTTGATACCAAAAAGATCATGGCA
CGTAGAGGGATGAATTTGTTTGAATTTGAGGATCTTTATTTGAGAAAACTGTATGGAAAGTGTACGGAGTAA

Upstream 100 bases:

>100_bases
CATTCCTTTACAGTTGTTATCGTATCACATAGCAATCCTTAGAGGGTGCAATGTGGACCAACCTAGGAACTTAGCAAAAT
CTGTTACAGTGGAGTGATTT

Downstream 100 bases:

>100_bases
AACATGACACCTAGTTTACAGGCACATATTAATTCCGGTAAAACTGTTGAAATCGATGGTTTGAGTTTTTTTTATATTGA
AGAAGGAAAGGGTGACGAAA

Product: glucose-1-phosphate thymidylyltransferase

Products: diphosphate; dTDPglucose

Alternate protein names: Glucose-1-Phosphate Thymidylyltransferase; Sugar Phospate Transferase; Sugar-Phosphate Nucleotydyltransferase; Sugar Phosphate Nucleotydyl Transferase; Nucleoside-Diphosphate-Sugar Pyrophosphorylase; UDP-N-Acetylglucosamine Diphosphorylase; Transferase Hexapeptide Repeat Containing Protein; Transferase Hexapeptide Repeat; Transferase; N-Acetylglucosamine-1-Phosphate Uridyltransferase; Glucose-1-Phosphate Thymidylyltransferase-Like Protein; UDP-GlcNAc Pyrophosphorylase; Sugar Nucleotidyltransferase; Sugar-1-Phosphate Guanyl Transferase; Hexapeptide Transferase Family Protein; Bifunctional GlmU Protein; Transferase LpxA Family; Hexapeptide Repeat-Containing Protein; Translation Initiation Factor EIF2B Subunit

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MNLLFDDSKRNPSLEPLSRFHSFFEWNLGGMNLLEKLDRKYPGSKFFYKGPNPDFETLIFNRYPHVFPATLENYDSIYSS
NSFLPWELLGTVTSIIEDTLNIDKEWKRFRQKYKSKQSGFHIVGKDKHLYIHSGATIYPGVVFDTTHGPILIEDGVKISS
FSFLEGPLFIGKNCQIDNARITGGTLIGNQCRIGGEVENSIILDYTNKHHEGFLGHSFVSSWVNLGALSTTSDLKNNYGI
VKLKVGDAIVNTGTIKFGSIIGPFTKLAIGVMSNTGTVFDIASNIVESRIQGYVPAFTWIKPGERYRLEEFLFDTKKIMA
RRGMNLFEFEDLYLRKLYGKCTE

Sequences:

>Translated_343_residues
MNLLFDDSKRNPSLEPLSRFHSFFEWNLGGMNLLEKLDRKYPGSKFFYKGPNPDFETLIFNRYPHVFPATLENYDSIYSS
NSFLPWELLGTVTSIIEDTLNIDKEWKRFRQKYKSKQSGFHIVGKDKHLYIHSGATIYPGVVFDTTHGPILIEDGVKISS
FSFLEGPLFIGKNCQIDNARITGGTLIGNQCRIGGEVENSIILDYTNKHHEGFLGHSFVSSWVNLGALSTTSDLKNNYGI
VKLKVGDAIVNTGTIKFGSIIGPFTKLAIGVMSNTGTVFDIASNIVESRIQGYVPAFTWIKPGERYRLEEFLFDTKKIMA
RRGMNLFEFEDLYLRKLYGKCTE
>Mature_343_residues
MNLLFDDSKRNPSLEPLSRFHSFFEWNLGGMNLLEKLDRKYPGSKFFYKGPNPDFETLIFNRYPHVFPATLENYDSIYSS
NSFLPWELLGTVTSIIEDTLNIDKEWKRFRQKYKSKQSGFHIVGKDKHLYIHSGATIYPGVVFDTTHGPILIEDGVKISS
FSFLEGPLFIGKNCQIDNARITGGTLIGNQCRIGGEVENSIILDYTNKHHEGFLGHSFVSSWVNLGALSTTSDLKNNYGI
VKLKVGDAIVNTGTIKFGSIIGPFTKLAIGVMSNTGTVFDIASNIVESRIQGYVPAFTWIKPGERYRLEEFLFDTKKIMA
RRGMNLFEFEDLYLRKLYGKCTE

Specific function: Unknown

COG id: COG1208

COG function: function code MJ; Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.7.24

Molecular weight: Translated: 38928; Mature: 38928

Theoretical pI: Translated: 8.41; Mature: 8.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLLFDDSKRNPSLEPLSRFHSFFEWNLGGMNLLEKLDRKYPGSKFFYKGPNPDFETLIF
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEECCCCCCHHHHEE
NRYPHVFPATLENYDSIYSSNSFLPWELLGTVTSIIEDTLNIDKEWKRFRQKYKSKQSGF
CCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCE
HIVGKDKHLYIHSGATIYPGVVFDTTHGPILIEDGVKISSFSFLEGPLFIGKNCQIDNAR
EEEECCCEEEEECCCEEECCEEEECCCCCEEEECCCEEECEEEECCCEEECCCCEECCCE
ITGGTLIGNQCRIGGEVENSIILDYTNKHHEGFLGHSFVSSWVNLGALSTTSDLKNNYGI
ECCCEEECCCEEECCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCHHHHHCCCCE
VKLKVGDAIVNTGTIKFGSIIGPFTKLAIGVMSNTGTVFDIASNIVESRIQGYVPAFTWI
EEEEECCEEEECCCEEECHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEE
KPGERYRLEEFLFDTKKIMARRGMNLFEFEDLYLRKLYGKCTE
CCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNLLFDDSKRNPSLEPLSRFHSFFEWNLGGMNLLEKLDRKYPGSKFFYKGPNPDFETLIF
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEECCCCCCHHHHEE
NRYPHVFPATLENYDSIYSSNSFLPWELLGTVTSIIEDTLNIDKEWKRFRQKYKSKQSGF
CCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCE
HIVGKDKHLYIHSGATIYPGVVFDTTHGPILIEDGVKISSFSFLEGPLFIGKNCQIDNAR
EEEECCCEEEEECCCEEECCEEEECCCCCEEEECCCEEECEEEECCCEEECCCCEECCCE
ITGGTLIGNQCRIGGEVENSIILDYTNKHHEGFLGHSFVSSWVNLGALSTTSDLKNNYGI
ECCCEEECCCEEECCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCHHHHHCCCCE
VKLKVGDAIVNTGTIKFGSIIGPFTKLAIGVMSNTGTVFDIASNIVESRIQGYVPAFTWI
EEEEECCEEEECCCEEECHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEE
KPGERYRLEEFLFDTKKIMARRGMNLFEFEDLYLRKLYGKCTE
CCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTTP; alpha-D-glucose 1-phosphate

Specific reaction: dTTP + alpha-D-glucose 1-phosphate = diphosphate + dTDP-glucose

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA