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The map label for this gene is trmE

Identifier: 183219714

GI number: 183219714

Start: 284399

End: 285784

Strand: Reverse

Name: trmE

Synonym: LEPBI_I0290

Alternate gene names: 183219714

Gene position: 285784-284399 (Counterclockwise)

Preceding gene: 183219715

Following gene: 183219713

Centisome position: 7.94

GC content: 38.38

Gene sequence:

>1386_bases
TTGATTGATACCATAGCGGCATTGTCCACTGCCCAAGGCCCCGGAGCGATTGGTATCCTTCGGGTCTCGGGTTCCTTGGT
GATGCCTATCGCCCTTGCTGTTCTAGAAAAAAATCAAAAACCCCTCACAGAAACCTTCCTACAAAATCAAAAACGTTCTG
CCATCTTTTGCGACTTTGTCGAAAATGGAAAACCTCTAGACCAAATTGTATTCTTTTACTTTCCAGCGCCTAACTCTTAC
ACAGGTGAAGATTTAGCCGAATTTCATCTCCACGGAAATCCACTTTTATTAAAACGAGCCCTTCATGTGTTATTTGAAAA
GGGAGCACGACCTGCACAAAAAGGTGAATTTACGAAACGTGCCTATATGAATGGCAAAATCAATTTATCTGGCGCTGAAG
CGATCAGTCGATTGATTGAAGCACGATCCAAATATGAATTGGAACTCGCCCAAAAAAATGTATTTGGTGAAATCACAAAA
TTAAGTTCCAAAATTCGAAGCGATTTAATTTCACTCAAAGCAGAATGTGAAGCCGAAATTGATTTTTCTACAGAAGACCT
AACCTTCGAGAGTTTAGAAGAACGAAAAAATAGAATGGTGGCTCTAAAAAATTTATGTTCTAAGCTCATCAAAGACTCTG
AAAGAGCGGAATCTTACATATTACAATCTACTGTAGTATTGTATGGAGAACCTAACACTGGAAAGTCGAGTCTTATGAAT
TTGCTTATCGGAAAGGATCGATCCATTATTTCCGATGTTCCTGGAACAACTAGGGATTATATTGCCGAAGAATTGAGTTT
GGATGGAATCCCTATCCGGCTTGTTGACACAGCAGGAATCCGGGATACAACCGACAACATCGAACAGATGGGCATTGAAC
GGAGCAAAAGAGAAGCAGATAGTGCCAATGTTAAATTATTTTTAATCGATACATCTCTTCCATTTGAAAAACAATCATTC
CTTCTCAAACACAAAGATCGCCTATTCGGTTCCCTTATTGTTGCCAACAAAATAGATTCAAAACATCCTTCTTGGCATAC
GGAATCTATTCATGATATCCAAGAGGAATTCCAACTCACTATTTCAGAAATTTCCTGTAAAACAAAACAAGGGATACCCG
AATTATTAGAATTGTTAAAATCAAAATTAACATCCAAAGATGATACGGAAGACTTGGTCTTATTAGAAGACCGCCAACGG
TATCACATTCAAAAAATTGAATCTTGTTTATCGGAAGCCATCCAATTGATGGAAAACAACGCTCCCGCAGAAATTTATAT
CCAGGAAATTAATGTCGCTTTGCATGAAATTGGCCAAGTGAATGGAGTTGTGGAAAATGAGGAAATTTTGGGTCGAATTT
TCAGCAAATTTTGTGTCGGAAAATAA

Upstream 100 bases:

>100_bases
ATTTCTGCCAAAGAAAAACACAAATACAAAGATTTGGAAGATCCATCTAAAAAAGGTTTACCTGTTGAAGATTTTGCCGA
TGGAGTCGACCAAGAAGATC

Downstream 100 bases:

>100_bases
ATCGGTTCTAGTCAAAAATTTAAGATTGTATAATCACCGAATTAGTATCTAATTGGTCGCACATTCGATACGAGGTAAAA
AATGAAAAAATTTCTTGTGA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MIDTIAALSTAQGPGAIGILRVSGSLVMPIALAVLEKNQKPLTETFLQNQKRSAIFCDFVENGKPLDQIVFFYFPAPNSY
TGEDLAEFHLHGNPLLLKRALHVLFEKGARPAQKGEFTKRAYMNGKINLSGAEAISRLIEARSKYELELAQKNVFGEITK
LSSKIRSDLISLKAECEAEIDFSTEDLTFESLEERKNRMVALKNLCSKLIKDSERAESYILQSTVVLYGEPNTGKSSLMN
LLIGKDRSIISDVPGTTRDYIAEELSLDGIPIRLVDTAGIRDTTDNIEQMGIERSKREADSANVKLFLIDTSLPFEKQSF
LLKHKDRLFGSLIVANKIDSKHPSWHTESIHDIQEEFQLTISEISCKTKQGIPELLELLKSKLTSKDDTEDLVLLEDRQR
YHIQKIESCLSEAIQLMENNAPAEIYIQEINVALHEIGQVNGVVENEEILGRIFSKFCVGK

Sequences:

>Translated_461_residues
MIDTIAALSTAQGPGAIGILRVSGSLVMPIALAVLEKNQKPLTETFLQNQKRSAIFCDFVENGKPLDQIVFFYFPAPNSY
TGEDLAEFHLHGNPLLLKRALHVLFEKGARPAQKGEFTKRAYMNGKINLSGAEAISRLIEARSKYELELAQKNVFGEITK
LSSKIRSDLISLKAECEAEIDFSTEDLTFESLEERKNRMVALKNLCSKLIKDSERAESYILQSTVVLYGEPNTGKSSLMN
LLIGKDRSIISDVPGTTRDYIAEELSLDGIPIRLVDTAGIRDTTDNIEQMGIERSKREADSANVKLFLIDTSLPFEKQSF
LLKHKDRLFGSLIVANKIDSKHPSWHTESIHDIQEEFQLTISEISCKTKQGIPELLELLKSKLTSKDDTEDLVLLEDRQR
YHIQKIESCLSEAIQLMENNAPAEIYIQEINVALHEIGQVNGVVENEEILGRIFSKFCVGK
>Mature_461_residues
MIDTIAALSTAQGPGAIGILRVSGSLVMPIALAVLEKNQKPLTETFLQNQKRSAIFCDFVENGKPLDQIVFFYFPAPNSY
TGEDLAEFHLHGNPLLLKRALHVLFEKGARPAQKGEFTKRAYMNGKINLSGAEAISRLIEARSKYELELAQKNVFGEITK
LSSKIRSDLISLKAECEAEIDFSTEDLTFESLEERKNRMVALKNLCSKLIKDSERAESYILQSTVVLYGEPNTGKSSLMN
LLIGKDRSIISDVPGTTRDYIAEELSLDGIPIRLVDTAGIRDTTDNIEQMGIERSKREADSANVKLFLIDTSLPFEKQSF
LLKHKDRLFGSLIVANKIDSKHPSWHTESIHDIQEEFQLTISEISCKTKQGIPELLELLKSKLTSKDDTEDLVLLEDRQR
YHIQKIESCLSEAIQLMENNAPAEIYIQEINVALHEIGQVNGVVENEEILGRIFSKFCVGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=476, Percent_Identity=32.1428571428571, Blast_Score=208, Evalue=7e-54,
Organism=Homo sapiens, GI193082993, Length=463, Percent_Identity=32.1814254859611, Blast_Score=203, Evalue=2e-52,
Organism=Homo sapiens, GI193082988, Length=508, Percent_Identity=30.3149606299213, Blast_Score=196, Evalue=3e-50,
Organism=Escherichia coli, GI2367268, Length=476, Percent_Identity=33.4033613445378, Blast_Score=230, Evalue=2e-61,
Organism=Escherichia coli, GI87082120, Length=124, Percent_Identity=34.6774193548387, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17507259, Length=470, Percent_Identity=31.063829787234, Blast_Score=181, Evalue=7e-46,
Organism=Saccharomyces cerevisiae, GI6323665, Length=503, Percent_Identity=30.8151093439364, Blast_Score=184, Evalue=2e-47,
Organism=Drosophila melanogaster, GI45550813, Length=492, Percent_Identity=28.4552845528455, Blast_Score=164, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_LEPBA (B0SAE4)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001961404.1
- ProteinModelPortal:   B0SAE4
- SMR:   B0SAE4
- GeneID:   6388839
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_0281
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- ProtClustDB:   PRK05291
- BioCyc:   LBIF355278:LBF_0281-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 51676; Mature: 51676

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: BINDING 21-21 BINDING 87-87 BINDING 126-126 BINDING 461-461

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDTIAALSTAQGPGAIGILRVSGSLVMPIALAVLEKNQKPLTETFLQNQKRSAIFCDFV
CCCCHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCHHHHHHCCCCCCEEEEEEC
ENGKPLDQIVFFYFPAPNSYTGEDLAEFHLHGNPLLLKRALHVLFEKGARPAQKGEFTKR
CCCCCHHHEEEEEECCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHH
AYMNGKINLSGAEAISRLIEARSKYELELAQKNVFGEITKLSSKIRSDLISLKAECEAEI
HHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
DFSTEDLTFESLEERKNRMVALKNLCSKLIKDSERAESYILQSTVVLYGEPNTGKSSLMN
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEEEEEEEECCCCCHHHHHH
LLIGKDRSIISDVPGTTRDYIAEELSLDGIPIRLVDTAGIRDTTDNIEQMGIERSKREAD
HHHCCCCHHHHCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHCCHHHHHCCC
SANVKLFLIDTSLPFEKQSFLLKHKDRLFGSLIVANKIDSKHPSWHTESIHDIQEEFQLT
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCHHHHHHHHHHHHH
ISEISCKTKQGIPELLELLKSKLTSKDDTEDLVLLEDRQRYHIQKIESCLSEAIQLMENN
HHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCC
APAEIYIQEINVALHEIGQVNGVVENEEILGRIFSKFCVGK
CCCEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIDTIAALSTAQGPGAIGILRVSGSLVMPIALAVLEKNQKPLTETFLQNQKRSAIFCDFV
CCCCHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCHHHHHHCCCCCCEEEEEEC
ENGKPLDQIVFFYFPAPNSYTGEDLAEFHLHGNPLLLKRALHVLFEKGARPAQKGEFTKR
CCCCCHHHEEEEEECCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHH
AYMNGKINLSGAEAISRLIEARSKYELELAQKNVFGEITKLSSKIRSDLISLKAECEAEI
HHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
DFSTEDLTFESLEERKNRMVALKNLCSKLIKDSERAESYILQSTVVLYGEPNTGKSSLMN
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEEEEEEEECCCCCHHHHHH
LLIGKDRSIISDVPGTTRDYIAEELSLDGIPIRLVDTAGIRDTTDNIEQMGIERSKREAD
HHHCCCCHHHHCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHCCHHHHHCCC
SANVKLFLIDTSLPFEKQSFLLKHKDRLFGSLIVANKIDSKHPSWHTESIHDIQEEFQLT
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCHHHHHHHHHHHHH
ISEISCKTKQGIPELLELLKSKLTSKDDTEDLVLLEDRQRYHIQKIESCLSEAIQLMENN
HHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCC
APAEIYIQEINVALHEIGQVNGVVENEEILGRIFSKFCVGK
CCCEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA