Click here to switch to the map view.

The map label for this gene is ligA

Identifier: 183219642

GI number: 183219642

Start: 207283

End: 209298

Strand: Reverse

Name: ligA

Synonym: LEPBI_I0217

Alternate gene names: 183219642

Gene position: 209298-207283 (Counterclockwise)

Preceding gene: 183219643

Following gene: 183219641

Centisome position: 5.81

GC content: 40.28

Gene sequence:

>2016_bases
TTGGCTAAAAAAACAAAAGCCGAGGATCCTAAAAAACGAATCATTACACTTCGTAAAGAAATTGGTCGTCATAATGATTT
GTATTACAAAGAAAATGCACCTGTCATCACTGACAAAGAATTTGATATCCTTGTCAAAGAACTCCAAAAACTCGAAAGTG
AAAATCCAGATTTAGCTGACGTATCCTCTCCCACCGCACAAGTAGGATCTGACCTAAGCCCCCAATTTAGCAAATTCAAA
CACAAAGTACCTGTATTATCCTTAGAAAACACATATAACGAAACCGAACTCTCAGAGTGGTTAGAAAAAACGGGGATCGA
AGAAAACTATTCCCTGGAATGGAAAATTGATGGCGCCTCTATCTTGTTATACTACGAAAAGGGAAAACTCACCAATTGTG
TGACAAGAGGTTCTGGTGGAATCGGTGATGTCGTCACAGAAAACGTAAAAACCATTTCAACCATCCCACATACTCTTTCT
GAAGAGATGAACCTGACTGTCCGCGGAGAAATTTTTATGACCTTCGCTGATTTTGAAGAATTCAACGAAGAATATGGTGG
CAAGTTTGCCAATCCTAGGAATTTGGCGGCAGGTTCGATCAAACAAAAAGACCCATTAGATGTCGCCAAACGTCCGTTAC
GAATCTATGTATATGATGTTTATTTTTCTAGCTCTAGAAAAGGGATCAATACTCATAAAGACATTTTAAGTTTATTAAAA
AAAGAAAAATTCCCTCTCGCACCAGATACTACGATCCTTACTGGTAAAAAACTCCTAAGAGAAATTGAATCCTTTCGCAA
AAAGAAAGATAAAATGCCATTCCCCGTCGATGGACTTGTCATCAAATTGGATTCGTTGAACTTACGAGAAAGTTTGGGTG
AAACAAGCCATTCTCCAAGGTGGGCAAGGGCATTTAAATTTGATGCCTTACTCAAGGAATCAACGATCGAAGAAATTGAT
TTTGCCATTGGTCGCACAGGAAAAGTCACGCCACGTGCCAAAGTGACTCCGATTTCCCTTGCAGGAACCACGGTCACTTA
CGCCACCTTACACAACCAAGACTATATTAACCAACTCGGTGCCGGCATTGGAGCAAAAGTACTGATCTCCAAACGAGGAG
AAATCATTCCTGCCGTGGAAAAAGTGACCTTTCCACCAAAAACGATATTTGTCCTACCAAACCAATGTCCATCGTGTAAC
ACAAAGTTAACGAAAGTGGACGATTCAGTGGATTTCTTTTGCACAAACAGGCATTGCCCAGAACGAAAGCTCAACCAACT
CATCTTTTTTTGTAGTAAAAAACAGATGAACATCGAAGGTCTCGGGGAAAGGCAAATTCAGATTTTTTTTGAGAAAGGTT
GGGTCAAAGACATTCCCGATTTATATACATTAGAAAAATATAAACCAACCTTACTTGAGTTAGATGGATTTGGTGAAAAA
TCAGTCAAAATCATATTCGATGCCATAGAAAAATCCAAAGAAAAAGACTTTCGTTTCACACTTCCTTCTATCGGCCTAAA
TGAAGTAGGCCCCAAAGTCACAGAAATTCTCATTGAAAATGGATATGATTCCTGGGACAAACTCCTCACCCTTTCCAAAT
CCAAAACAGCAAACGAAGACCTAAAAGCCATTCATGGAATTGGCCCAAGAACCATCGAAGCTTTACTCACTCATCTCAAA
GACAAAGAAACTTTGAAATTAGTCGCCACGCTCAAGAAACTCGGACTTAAATTCCAAGCAGACGAAACCGAAAAAAGCGA
CTTGCAACCGTTTGTTGGTCAAAGTTGGTGTGTCACAGGAAGTTTTGAAAACTTCCAACCTCGTGACCTTGCCATGGACC
TCATTACGAAACATGGTGGCAAAAAAGTAACAAGTGTTTCCTCTAAAACCACTCACTTACTCTACGGCCCAGGCGCCGGC
TCCAAATTGGACAAGGCCACGGAACTGGGTGTAAAACTTGTCACGGAGTCAGAGTTTTTGGATTTATTGAAACAGGAAGG
GATAGCGATTGATTAA

Upstream 100 bases:

>100_bases
CACTGGTAACGTAACAGGGCCTCACCTACATTATGAAATTTTCATTTCGGAAGAAGGAAAAACCGATCCAGAACAGTACA
TGCAAGCTGGAGTTTACTGA

Downstream 100 bases:

>100_bases
TGGAGGAGTGCAATCCTAGAAAGGTCGCTTCTTTGAATTATTGAAATCAGTGAAGTCCTATGTTGTTAAAACATATAATA
AATCTTGTTTTTAAAAGGCT

Product: DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 671; Mature: 670

Protein sequence:

>671_residues
MAKKTKAEDPKKRIITLRKEIGRHNDLYYKENAPVITDKEFDILVKELQKLESENPDLADVSSPTAQVGSDLSPQFSKFK
HKVPVLSLENTYNETELSEWLEKTGIEENYSLEWKIDGASILLYYEKGKLTNCVTRGSGGIGDVVTENVKTISTIPHTLS
EEMNLTVRGEIFMTFADFEEFNEEYGGKFANPRNLAAGSIKQKDPLDVAKRPLRIYVYDVYFSSSRKGINTHKDILSLLK
KEKFPLAPDTTILTGKKLLREIESFRKKKDKMPFPVDGLVIKLDSLNLRESLGETSHSPRWARAFKFDALLKESTIEEID
FAIGRTGKVTPRAKVTPISLAGTTVTYATLHNQDYINQLGAGIGAKVLISKRGEIIPAVEKVTFPPKTIFVLPNQCPSCN
TKLTKVDDSVDFFCTNRHCPERKLNQLIFFCSKKQMNIEGLGERQIQIFFEKGWVKDIPDLYTLEKYKPTLLELDGFGEK
SVKIIFDAIEKSKEKDFRFTLPSIGLNEVGPKVTEILIENGYDSWDKLLTLSKSKTANEDLKAIHGIGPRTIEALLTHLK
DKETLKLVATLKKLGLKFQADETEKSDLQPFVGQSWCVTGSFENFQPRDLAMDLITKHGGKKVTSVSSKTTHLLYGPGAG
SKLDKATELGVKLVTESEFLDLLKQEGIAID

Sequences:

>Translated_671_residues
MAKKTKAEDPKKRIITLRKEIGRHNDLYYKENAPVITDKEFDILVKELQKLESENPDLADVSSPTAQVGSDLSPQFSKFK
HKVPVLSLENTYNETELSEWLEKTGIEENYSLEWKIDGASILLYYEKGKLTNCVTRGSGGIGDVVTENVKTISTIPHTLS
EEMNLTVRGEIFMTFADFEEFNEEYGGKFANPRNLAAGSIKQKDPLDVAKRPLRIYVYDVYFSSSRKGINTHKDILSLLK
KEKFPLAPDTTILTGKKLLREIESFRKKKDKMPFPVDGLVIKLDSLNLRESLGETSHSPRWARAFKFDALLKESTIEEID
FAIGRTGKVTPRAKVTPISLAGTTVTYATLHNQDYINQLGAGIGAKVLISKRGEIIPAVEKVTFPPKTIFVLPNQCPSCN
TKLTKVDDSVDFFCTNRHCPERKLNQLIFFCSKKQMNIEGLGERQIQIFFEKGWVKDIPDLYTLEKYKPTLLELDGFGEK
SVKIIFDAIEKSKEKDFRFTLPSIGLNEVGPKVTEILIENGYDSWDKLLTLSKSKTANEDLKAIHGIGPRTIEALLTHLK
DKETLKLVATLKKLGLKFQADETEKSDLQPFVGQSWCVTGSFENFQPRDLAMDLITKHGGKKVTSVSSKTTHLLYGPGAG
SKLDKATELGVKLVTESEFLDLLKQEGIAID
>Mature_670_residues
AKKTKAEDPKKRIITLRKEIGRHNDLYYKENAPVITDKEFDILVKELQKLESENPDLADVSSPTAQVGSDLSPQFSKFKH
KVPVLSLENTYNETELSEWLEKTGIEENYSLEWKIDGASILLYYEKGKLTNCVTRGSGGIGDVVTENVKTISTIPHTLSE
EMNLTVRGEIFMTFADFEEFNEEYGGKFANPRNLAAGSIKQKDPLDVAKRPLRIYVYDVYFSSSRKGINTHKDILSLLKK
EKFPLAPDTTILTGKKLLREIESFRKKKDKMPFPVDGLVIKLDSLNLRESLGETSHSPRWARAFKFDALLKESTIEEIDF
AIGRTGKVTPRAKVTPISLAGTTVTYATLHNQDYINQLGAGIGAKVLISKRGEIIPAVEKVTFPPKTIFVLPNQCPSCNT
KLTKVDDSVDFFCTNRHCPERKLNQLIFFCSKKQMNIEGLGERQIQIFFEKGWVKDIPDLYTLEKYKPTLLELDGFGEKS
VKIIFDAIEKSKEKDFRFTLPSIGLNEVGPKVTEILIENGYDSWDKLLTLSKSKTANEDLKAIHGIGPRTIEALLTHLKD
KETLKLVATLKKLGLKFQADETEKSDLQPFVGQSWCVTGSFENFQPRDLAMDLITKHGGKKVTSVSSKTTHLLYGPGAGS
KLDKATELGVKLVTESEFLDLLKQEGIAID

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=678, Percent_Identity=34.5132743362832, Blast_Score=363, Evalue=1e-101,
Organism=Escherichia coli, GI87082305, Length=448, Percent_Identity=23.6607142857143, Blast_Score=112, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_LEPBA (B0SA75)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001961335.1
- ProteinModelPortal:   B0SA75
- GeneID:   6387819
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_0211
- HOGENOM:   HBG620317
- OMA:   HNQDYID
- ProtClustDB:   CLSK575130
- BioCyc:   LBIF355278:LBF_0211-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 75388; Mature: 75257

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 115-115 BINDING 113-113 BINDING 136-136 BINDING 170-170 BINDING 282-282 BINDING 306-306

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKTKAEDPKKRIITLRKEIGRHNDLYYKENAPVITDKEFDILVKELQKLESENPDLAD
CCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCEEECCHHHHHHHHHHHHHCCCCCCCC
VSSPTAQVGSDLSPQFSKFKHKVPVLSLENTYNETELSEWLEKTGIEENYSLEWKIDGAS
CCCCHHHHCCCCCHHHHHHHHCCCEEEECCCCCHHHHHHHHHHCCCCCCCEEEEEECCEE
ILLYYEKGKLTNCVTRGSGGIGDVVTENVKTISTIPHTLSEEMNLTVRGEIFMTFADFEE
EEEEEECCCEEEHEECCCCCCCHHHHCCHHHHHHCCHHHHHHCCEEEEEEEEEEEHHHHH
FNEEYGGKFANPRNLAAGSIKQKDPLDVAKRPLRIYVYDVYFSSSRKGINTHKDILSLLK
HHHHHCCCCCCCCCCCCCCCCCCCCHHHHCCCEEEEEEEEEECCCCCCCCHHHHHHHHHH
KEKFPLAPDTTILTGKKLLREIESFRKKKDKMPFPVDGLVIKLDSLNLRESLGETSHSPR
HCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHCCCCCCCC
WARAFKFDALLKESTIEEIDFAIGRTGKVTPRAKVTPISLAGTTVTYATLHNQDYINQLG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECEEEECCCEEEEEEECCHHHHHHHC
AGIGAKVLISKRGEIIPAVEKVTFPPKTIFVLPNQCPSCNTKLTKVDDSVDFFCTNRHCP
CCCCEEEEEECCCCEEEHHHHCCCCCCEEEEECCCCCCCCCEEEEECCCCEEEECCCCCC
ERKLNQLIFFCSKKQMNIEGLGERQIQIFFEKGWVKDIPDLYTLEKYKPTLLELDGFGEK
HHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHCCCCEEEECCCCCC
SVKIIFDAIEKSKEKDFRFTLPSIGLNEVGPKVTEILIENGYDSWDKLLTLSKSKTANED
HHHHHHHHHHHCCCCCEEEECCCCCHHHCCHHHHHHHHHCCCCCHHHHEEECCCCCCCHH
LKAIHGIGPRTIEALLTHLKDKETLKLVATLKKLGLKFQADETEKSDLQPFVGQSWCVTG
HHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCEEEEC
SFENFQPRDLAMDLITKHGGKKVTSVSSKTTHLLYGPGAGSKLDKATELGVKLVTESEFL
CCCCCCHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCCCCCHHHHHHCCEEEEEHHHHH
DLLKQEGIAID
HHHHHCCCCCC
>Mature Secondary Structure 
AKKTKAEDPKKRIITLRKEIGRHNDLYYKENAPVITDKEFDILVKELQKLESENPDLAD
CCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCEEECCHHHHHHHHHHHHHCCCCCCCC
VSSPTAQVGSDLSPQFSKFKHKVPVLSLENTYNETELSEWLEKTGIEENYSLEWKIDGAS
CCCCHHHHCCCCCHHHHHHHHCCCEEEECCCCCHHHHHHHHHHCCCCCCCEEEEEECCEE
ILLYYEKGKLTNCVTRGSGGIGDVVTENVKTISTIPHTLSEEMNLTVRGEIFMTFADFEE
EEEEEECCCEEEHEECCCCCCCHHHHCCHHHHHHCCHHHHHHCCEEEEEEEEEEEHHHHH
FNEEYGGKFANPRNLAAGSIKQKDPLDVAKRPLRIYVYDVYFSSSRKGINTHKDILSLLK
HHHHHCCCCCCCCCCCCCCCCCCCCHHHHCCCEEEEEEEEEECCCCCCCCHHHHHHHHHH
KEKFPLAPDTTILTGKKLLREIESFRKKKDKMPFPVDGLVIKLDSLNLRESLGETSHSPR
HCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHCCCCCCCC
WARAFKFDALLKESTIEEIDFAIGRTGKVTPRAKVTPISLAGTTVTYATLHNQDYINQLG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECEEEECCCEEEEEEECCHHHHHHHC
AGIGAKVLISKRGEIIPAVEKVTFPPKTIFVLPNQCPSCNTKLTKVDDSVDFFCTNRHCP
CCCCEEEEEECCCCEEEHHHHCCCCCCEEEEECCCCCCCCCEEEEECCCCEEEECCCCCC
ERKLNQLIFFCSKKQMNIEGLGERQIQIFFEKGWVKDIPDLYTLEKYKPTLLELDGFGEK
HHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHCCCCEEEECCCCCC
SVKIIFDAIEKSKEKDFRFTLPSIGLNEVGPKVTEILIENGYDSWDKLLTLSKSKTANED
HHHHHHHHHHHCCCCCEEEECCCCCHHHCCHHHHHHHHHCCCCCHHHHEEECCCCCCCHH
LKAIHGIGPRTIEALLTHLKDKETLKLVATLKKLGLKFQADETEKSDLQPFVGQSWCVTG
HHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCEEEEC
SFENFQPRDLAMDLITKHGGKKVTSVSSKTTHLLYGPGAGSKLDKATELGVKLVTESEFL
CCCCCCHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCCCCCHHHHHHCCEEEEEHHHHH
DLLKQEGIAID
HHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA