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The map label for this gene is nadB [H]

Identifier: 182680393

GI number: 182680393

Start: 3969804

End: 3971372

Strand: Reverse

Name: nadB [H]

Synonym: Bind_3493

Alternate gene names: 182680393

Gene position: 3971372-3969804 (Counterclockwise)

Preceding gene: 182680394

Following gene: 182680392

Centisome position: 95.23

GC content: 64.31

Gene sequence:

>1569_bases
ATGACCCAGTCCCGGATGACCGAATTTCACGATGTCGGGGGCGCACCGATCATCATCGGCGGCGGCATTGCGGGGCTGAT
GACGGCGCTGACACTGGCGCCGCGGCCCGTCGTCCTTCTGGCCAAATCGCCGCTTGGCCATGAAGCGTCGAGCGTGCTCG
CGCAGGGCGGCGTTGCCGTGAGCCTTGACGCCGATGATAGCGTCGATCTCCATCTCGCCGATACTTTGGCGGCGGGCGAT
GGTCTTTGCGATGCGGCGGCGGCGCGCGCCATCCTTGCCGCCGGGCCCGCCATGATCGAGGAATTGGTGCGCCTCGGCGT
GCCTTTCGACCGCGATGCCAAGGGCGAACTTGCGCTCGGCCTTGAGGCTGCTCATAGCCGGAGGCGCATCGTTCATGCGG
GCGGTGATTCGACGGGGCACGCGATCGTCAAAACCTTGATGGCCCGCATTCAGGCAACGCCTTCGGTCACTTTGATCGAA
CGGGTAGCAGCGCGCCGGATCCTCGTCGAGGATGGGGGCGTGGTTGGCGTCCTCGCCAATACGCATTGCGGTCCGGTGGT
GTTTCCGACCGATAAAGTGATCATTGCAACAGGCGGCATCGGTGCCCTTTATGAATATGGGACCAATCCCGCCGGTTCTT
TTGGCCAGGGGCTGGCGCTCGCTGCGCGGGCTGGTGCGATCATGGGCGATCTTGAATTCGTTCAGTTCCATCCGACCGCG
CTCGATAGCGATTCATTTCCGCTGAAACTGATCAGCGAGGCGGTGCGCGGCGAAGGCGCCATCCTTGTCGATGAGCAAGG
GCATCGTTTCATGAGTGGCGTGCCGGGGGGGGAACTCGCCCCGCGCGATGTCGTGGCGCGCGCCGTCTGGAAACAGATGC
AGGCGGGACATAAAGTCTTTCTCGATGCGCGTGAAAAACCGGGCGTGGATTTTCCAAAGCGTTTCCCGGCCGTCACCGCC
TTGTGCAAGGCCGCGGGCATCGATCCCGTGCGCCAGCCGATCCCGATCCGTCCGGCGGCGCATTATCATATGGGCGGCAT
CAAGGTGGATGGTTCAGGCCGCTCCTCGATCGACGGCCTTTGGGCTTGCGGCGAGGCCGCCTGCACGGGCCTCCACGGCG
CCAATCGGCTGGCCAGCAATTCGCTCCTCGAAGCCGCTGTCTGCGGCCGCCGCGTTGCCGAAGATATTGCGGGCGGCAAG
ATCAAGGGCAAGTCACTGCTCCGGGCGCGAGCCGAGCGGCAGGCCAATGCCGTGCCGGCACCGGGGCCGAGCGATCCTGC
ATTGGTGCGGCCGATTGTTTCGCGCGGAATCGGCGTGTTGCGGGACGCTCAAGGCCTGCGCGAAGCCATAGCGGATCTTT
ATCCGCTCGCGACCGGCGAAACACCCGCCAGTGATGCGGCGACAGTCGGCCTGATGATCGCCGTTGCAGCACTTCGGCGA
GAGGAAAGCCGCGGTGCACATATGCGCACGGATTTCCCCAACCACGCCTCCGAAGCCAGGCGCACGAGCCTGTGTCTTGA
GGATGCTTTGGCGCAAGCGCGTGAAAATTTGCCGGAACCCGTCGTTTAA

Upstream 100 bases:

>100_bases
ACATCCGCCATGCGCTGGAGACGATGACGCATGAAGTGACGATCGACCCGGAAGTGGCCGGGCGGGCGCGTCTTGCCGTC
GAGCGCATGCTGGCCGTGAA

Downstream 100 bases:

>100_bases
TCCCAGGACCAATCCATGACGAGCGCGCCCGCTCCTATCTCCCCGCAATTACCTCGTCTCCTGGTTGAGCCCCTGGTGCG
TGCCGCGCTCCTCGAAGATC

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 522; Mature: 521

Protein sequence:

>522_residues
MTQSRMTEFHDVGGAPIIIGGGIAGLMTALTLAPRPVVLLAKSPLGHEASSVLAQGGVAVSLDADDSVDLHLADTLAAGD
GLCDAAAARAILAAGPAMIEELVRLGVPFDRDAKGELALGLEAAHSRRRIVHAGGDSTGHAIVKTLMARIQATPSVTLIE
RVAARRILVEDGGVVGVLANTHCGPVVFPTDKVIIATGGIGALYEYGTNPAGSFGQGLALAARAGAIMGDLEFVQFHPTA
LDSDSFPLKLISEAVRGEGAILVDEQGHRFMSGVPGGELAPRDVVARAVWKQMQAGHKVFLDAREKPGVDFPKRFPAVTA
LCKAAGIDPVRQPIPIRPAAHYHMGGIKVDGSGRSSIDGLWACGEAACTGLHGANRLASNSLLEAAVCGRRVAEDIAGGK
IKGKSLLRARAERQANAVPAPGPSDPALVRPIVSRGIGVLRDAQGLREAIADLYPLATGETPASDAATVGLMIAVAALRR
EESRGAHMRTDFPNHASEARRTSLCLEDALAQARENLPEPVV

Sequences:

>Translated_522_residues
MTQSRMTEFHDVGGAPIIIGGGIAGLMTALTLAPRPVVLLAKSPLGHEASSVLAQGGVAVSLDADDSVDLHLADTLAAGD
GLCDAAAARAILAAGPAMIEELVRLGVPFDRDAKGELALGLEAAHSRRRIVHAGGDSTGHAIVKTLMARIQATPSVTLIE
RVAARRILVEDGGVVGVLANTHCGPVVFPTDKVIIATGGIGALYEYGTNPAGSFGQGLALAARAGAIMGDLEFVQFHPTA
LDSDSFPLKLISEAVRGEGAILVDEQGHRFMSGVPGGELAPRDVVARAVWKQMQAGHKVFLDAREKPGVDFPKRFPAVTA
LCKAAGIDPVRQPIPIRPAAHYHMGGIKVDGSGRSSIDGLWACGEAACTGLHGANRLASNSLLEAAVCGRRVAEDIAGGK
IKGKSLLRARAERQANAVPAPGPSDPALVRPIVSRGIGVLRDAQGLREAIADLYPLATGETPASDAATVGLMIAVAALRR
EESRGAHMRTDFPNHASEARRTSLCLEDALAQARENLPEPVV
>Mature_521_residues
TQSRMTEFHDVGGAPIIIGGGIAGLMTALTLAPRPVVLLAKSPLGHEASSVLAQGGVAVSLDADDSVDLHLADTLAAGDG
LCDAAAARAILAAGPAMIEELVRLGVPFDRDAKGELALGLEAAHSRRRIVHAGGDSTGHAIVKTLMARIQATPSVTLIER
VAARRILVEDGGVVGVLANTHCGPVVFPTDKVIIATGGIGALYEYGTNPAGSFGQGLALAARAGAIMGDLEFVQFHPTAL
DSDSFPLKLISEAVRGEGAILVDEQGHRFMSGVPGGELAPRDVVARAVWKQMQAGHKVFLDAREKPGVDFPKRFPAVTAL
CKAAGIDPVRQPIPIRPAAHYHMGGIKVDGSGRSSIDGLWACGEAACTGLHGANRLASNSLLEAAVCGRRVAEDIAGGKI
KGKSLLRARAERQANAVPAPGPSDPALVRPIVSRGIGVLRDAQGLREAIADLYPLATGETPASDAATVGLMIAVAALRRE
ESRGAHMRTDFPNHASEARRTSLCLEDALAQARENLPEPVV

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=380, Percent_Identity=34.4736842105263, Blast_Score=182, Evalue=7e-46,
Organism=Escherichia coli, GI1788928, Length=523, Percent_Identity=36.5200764818356, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI1790597, Length=498, Percent_Identity=32.5301204819277, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI1786942, Length=517, Percent_Identity=31.1411992263056, Blast_Score=186, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI17505833, Length=514, Percent_Identity=30.1556420233463, Blast_Score=175, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI17550100, Length=513, Percent_Identity=30.0194931773879, Blast_Score=172, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI71986328, Length=447, Percent_Identity=30.6487695749441, Blast_Score=102, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6322701, Length=516, Percent_Identity=33.3333333333333, Blast_Score=206, Evalue=6e-54,
Organism=Saccharomyces cerevisiae, GI6322416, Length=509, Percent_Identity=32.0235756385069, Blast_Score=196, Evalue=9e-51,
Organism=Drosophila melanogaster, GI17137288, Length=509, Percent_Identity=31.041257367387, Blast_Score=177, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24655642, Length=509, Percent_Identity=31.041257367387, Blast_Score=177, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24655647, Length=509, Percent_Identity=31.041257367387, Blast_Score=177, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24663005, Length=514, Percent_Identity=29.3774319066148, Blast_Score=173, Evalue=3e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 54031; Mature: 53899

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQSRMTEFHDVGGAPIIIGGGIAGLMTALTLAPRPVVLLAKSPLGHEASSVLAQGGVAV
CCCHHHHHHHHCCCCCEEECCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHCCCEEE
SLDADDSVDLHLADTLAAGDGLCDAAAARAILAAGPAMIEELVRLGVPFDRDAKGELALG
EECCCCCCEEEEHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEC
LEAAHSRRRIVHAGGDSTGHAIVKTLMARIQATPSVTLIERVAARRILVEDGGVVGVLAN
HHHHHCCCEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHEEEEECCCEEEEEEC
THCGPVVFPTDKVIIATGGIGALYEYGTNPAGSFGQGLALAARAGAIMGDLEFVQFHPTA
CCCCCEEECCCEEEEEECCCHHHHHCCCCCCCCCCCCHHHHHHHCHHHCCCEEEEECCCC
LDSDSFPLKLISEAVRGEGAILVDEQGHRFMSGVPGGELAPRDVVARAVWKQMQAGHKVF
CCCCCCCHHHHHHHHCCCCEEEECCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEE
LDAREKPGVDFPKRFPAVTALCKAAGIDPVRQPIPIRPAAHYHMGGIKVDGSGRSSIDGL
EECCCCCCCCCCHHHHHHHHHHHHCCCCHHCCCCCCCCCCCEECCCEEECCCCCCCCCHH
WACGEAACTGLHGANRLASNSLLEAAVCGRRVAEDIAGGKIKGKSLLRARAERQANAVPA
HHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCC
PGPSDPALVRPIVSRGIGVLRDAQGLREAIADLYPLATGETPASDAATVGLMIAVAALRR
CCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
EESRGAHMRTDFPNHASEARRTSLCLEDALAQARENLPEPVV
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TQSRMTEFHDVGGAPIIIGGGIAGLMTALTLAPRPVVLLAKSPLGHEASSVLAQGGVAV
CCHHHHHHHHCCCCCEEECCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHCCCEEE
SLDADDSVDLHLADTLAAGDGLCDAAAARAILAAGPAMIEELVRLGVPFDRDAKGELALG
EECCCCCCEEEEHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEC
LEAAHSRRRIVHAGGDSTGHAIVKTLMARIQATPSVTLIERVAARRILVEDGGVVGVLAN
HHHHHCCCEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHEEEEECCCEEEEEEC
THCGPVVFPTDKVIIATGGIGALYEYGTNPAGSFGQGLALAARAGAIMGDLEFVQFHPTA
CCCCCEEECCCEEEEEECCCHHHHHCCCCCCCCCCCCHHHHHHHCHHHCCCEEEEECCCC
LDSDSFPLKLISEAVRGEGAILVDEQGHRFMSGVPGGELAPRDVVARAVWKQMQAGHKVF
CCCCCCCHHHHHHHHCCCCEEEECCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEE
LDAREKPGVDFPKRFPAVTALCKAAGIDPVRQPIPIRPAAHYHMGGIKVDGSGRSSIDGL
EECCCCCCCCCCHHHHHHHHHHHHCCCCHHCCCCCCCCCCCEECCCEEECCCCCCCCCHH
WACGEAACTGLHGANRLASNSLLEAAVCGRRVAEDIAGGKIKGKSLLRARAERQANAVPA
HHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCC
PGPSDPALVRPIVSRGIGVLRDAQGLREAIADLYPLATGETPASDAATVGLMIAVAALRR
CCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
EESRGAHMRTDFPNHASEARRTSLCLEDALAQARENLPEPVV
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]