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The map label for this gene is recF [H]

Identifier: 182680050

GI number: 182680050

Start: 3589413

End: 3590624

Strand: Reverse

Name: recF [H]

Synonym: Bind_3146

Alternate gene names: 182680050

Gene position: 3590624-3589413 (Counterclockwise)

Preceding gene: 182680053

Following gene: 182680049

Centisome position: 86.1

GC content: 65.18

Gene sequence:

>1212_bases
ATGTCCGAACACTGCGATGCCACCGGGCTTGCGATACCCCTCGTGCGGCGGCTGATTCTGTCTGATTTCCGCTCCTATCC
GGCGCTCGATCTGTCGCTTGGCGCCAAAATGATCGTCGTCACGGGTGAGAATGGCGCAGGTAAGACCAATCTGATCGAAG
CCTTGTCTCTCTTCACGCCTGGGCGCGGCCTGCGGCGGGTTGAACTTGCGTCCTGCGCCCGCGCGGAAGGGCCAGGCGGA
TTTGCGGTTTCGATCGAGATCGAGACGGGAGCGGAGAGGGTTCAACTCGGCACGGGTCTGGTGCCGCGGAGCGAGGGCGG
ATTTGCGCGTCAGTACCGGATCGAGCGCGCGCCCGTTGGCTCGGCGCGGAGTTTTGCCGATCATCTGCGGATCGTCTGGC
TGACTCCGGCGCAGGATGGGCTCTTCGCGGCGTCCCCCGGCGAGCGGCGGCGTTTCCTTGACCGGCTGGTGCTCTGTGTC
GATGCCGAGCATGGCGCCCGCGTCACGGTTTTGGAGCGCGCCTTGCGCAATCGCAATCGTCAGCTCGAGGATCATTATGC
CGATTCGCGCTGGCTCGATGCGACGGAAAAGGAAATCGCCGAAATCGCCGTGGCGGTCGCGGCGGCACGCGTGGAAACCG
TCGCTCGCCTGCGGCGGCTGATCGACGAGAGCCGCGATACGATCACGCCCTTTCCCTGGGCGGATCTCGAAATCCGGGGC
GATCTCGAACGGCTCGTCGGTGAGCGTCCGGCGCTGGAGGCGGAAGAGCTTTATCGGGGCATTCTGCGCGATAACCGGCG
GCGCGACGCGGCGGCCGGCCGGGCCTTGGTCGGGCCGCAAAACAGCGACCTTCTGGTGCGTCATGGGCCGAAACAGGCGG
ATGCGGCGCGCTCCTCGACGGGTGAACAAAAGGCCTTGCTGGTTGGTCTGGTGCTGGCCCATGCCCGTCTCGTTGCCCGC
GCCACGCAAACGGCTCCAATGATCCTGCTCGACGAGATCGCTGCCCATTTCGACGCTTCCCGCCGGGTCGCGCTTTATGA
GGAATTGGCGGCGCTTCCCGGACAAATCTGGATGACCGGCGCGGAAGCTTCCGCGTTTTCCGGCCTCGCCGATCATGCTG
ATCTGCTCCAGGTGAGGCCCGGTCTCGTGGGCCGGCTCGCGCATCAAAGCGACAGGATCCTCCCGGCATCCGATTCAACG
AGCGATGTTTGA

Upstream 100 bases:

>100_bases
GCATTGCCTAAGGGGGAATCGGCATAAACCACGATTCTACTCAAAAATCCTTTATAAGCCATCCACTCATGGCTCATTTC
GAATGCGATCAACCGGTCCC

Downstream 100 bases:

>100_bases
CGCGAGGCTCTTTCGGGATCGATTCGAGAGTCGAGGCGTTTTGTTCAAACTCAATTCAGCCTGATGGACTAGAATCGCGG
TTGATGATCCTTTTGTCAGG

Product: DNA replication and repair protein RecF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MSEHCDATGLAIPLVRRLILSDFRSYPALDLSLGAKMIVVTGENGAGKTNLIEALSLFTPGRGLRRVELASCARAEGPGG
FAVSIEIETGAERVQLGTGLVPRSEGGFARQYRIERAPVGSARSFADHLRIVWLTPAQDGLFAASPGERRRFLDRLVLCV
DAEHGARVTVLERALRNRNRQLEDHYADSRWLDATEKEIAEIAVAVAAARVETVARLRRLIDESRDTITPFPWADLEIRG
DLERLVGERPALEAEELYRGILRDNRRRDAAAGRALVGPQNSDLLVRHGPKQADAARSSTGEQKALLVGLVLAHARLVAR
ATQTAPMILLDEIAAHFDASRRVALYEELAALPGQIWMTGAEASAFSGLADHADLLQVRPGLVGRLAHQSDRILPASDST
SDV

Sequences:

>Translated_403_residues
MSEHCDATGLAIPLVRRLILSDFRSYPALDLSLGAKMIVVTGENGAGKTNLIEALSLFTPGRGLRRVELASCARAEGPGG
FAVSIEIETGAERVQLGTGLVPRSEGGFARQYRIERAPVGSARSFADHLRIVWLTPAQDGLFAASPGERRRFLDRLVLCV
DAEHGARVTVLERALRNRNRQLEDHYADSRWLDATEKEIAEIAVAVAAARVETVARLRRLIDESRDTITPFPWADLEIRG
DLERLVGERPALEAEELYRGILRDNRRRDAAAGRALVGPQNSDLLVRHGPKQADAARSSTGEQKALLVGLVLAHARLVAR
ATQTAPMILLDEIAAHFDASRRVALYEELAALPGQIWMTGAEASAFSGLADHADLLQVRPGLVGRLAHQSDRILPASDST
SDV
>Mature_402_residues
SEHCDATGLAIPLVRRLILSDFRSYPALDLSLGAKMIVVTGENGAGKTNLIEALSLFTPGRGLRRVELASCARAEGPGGF
AVSIEIETGAERVQLGTGLVPRSEGGFARQYRIERAPVGSARSFADHLRIVWLTPAQDGLFAASPGERRRFLDRLVLCVD
AEHGARVTVLERALRNRNRQLEDHYADSRWLDATEKEIAEIAVAVAAARVETVARLRRLIDESRDTITPFPWADLEIRGD
LERLVGERPALEAEELYRGILRDNRRRDAAAGRALVGPQNSDLLVRHGPKQADAARSSTGEQKALLVGLVLAHARLVARA
TQTAPMILLDEIAAHFDASRRVALYEELAALPGQIWMTGAEASAFSGLADHADLLQVRPGLVGRLAHQSDRILPASDSTS
DV

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=377, Percent_Identity=25.4641909814324, Blast_Score=85, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- InterPro:   IPR007406 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 43822; Mature: 43691

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEHCDATGLAIPLVRRLILSDFRSYPALDLSLGAKMIVVTGENGAGKTNLIEALSLFTP
CCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCCCCHHHHHHHHHHCCC
GRGLRRVELASCARAEGPGGFAVSIEIETGAERVQLGTGLVPRSEGGFARQYRIERAPVG
CCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCCEECCCCCCCCCCCCCHHHEEECCCCCC
SARSFADHLRIVWLTPAQDGLFAASPGERRRFLDRLVLCVDAEHGARVTVLERALRNRNR
CHHHHHCCEEEEEEECCCCCEEECCCCHHHHHHHHHHEEEECCCCCCHHHHHHHHHHCCC
QLEDHYADSRWLDATEKEIAEIAVAVAAARVETVARLRRLIDESRDTITPFPWADLEIRG
CHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECC
DLERLVGERPALEAEELYRGILRDNRRRDAAAGRALVGPQNSDLLVRHGPKQADAARSST
CHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHCCCEEECCCCCCEEEECCCCHHHHHHCCC
GEQKALLVGLVLAHARLVARATQTAPMILLDEIAAHFDASRRVALYEELAALPGQIWMTG
CCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEC
AEASAFSGLADHADLLQVRPGLVGRLAHQSDRILPASDSTSDV
CCHHHHCCHHHHHHHHHHCCCHHHHHHHCCCCEECCCCCCCCC
>Mature Secondary Structure 
SEHCDATGLAIPLVRRLILSDFRSYPALDLSLGAKMIVVTGENGAGKTNLIEALSLFTP
CCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCCCCHHHHHHHHHHCCC
GRGLRRVELASCARAEGPGGFAVSIEIETGAERVQLGTGLVPRSEGGFARQYRIERAPVG
CCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCCEECCCCCCCCCCCCCHHHEEECCCCCC
SARSFADHLRIVWLTPAQDGLFAASPGERRRFLDRLVLCVDAEHGARVTVLERALRNRNR
CHHHHHCCEEEEEEECCCCCEEECCCCHHHHHHHHHHEEEECCCCCCHHHHHHHHHHCCC
QLEDHYADSRWLDATEKEIAEIAVAVAAARVETVARLRRLIDESRDTITPFPWADLEIRG
CHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECC
DLERLVGERPALEAEELYRGILRDNRRRDAAAGRALVGPQNSDLLVRHGPKQADAARSST
CHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHCCCEEECCCCCCEEEECCCCHHHHHHCCC
GEQKALLVGLVLAHARLVARATQTAPMILLDEIAAHFDASRRVALYEELAALPGQIWMTG
CCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEC
AEASAFSGLADHADLLQVRPGLVGRLAHQSDRILPASDSTSDV
CCHHHHCCHHHHHHHHHHCCCHHHHHHHCCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA