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The map label for this gene is pyk [H]

Identifier: 182679770

GI number: 182679770

Start: 3260193

End: 3261626

Strand: Reverse

Name: pyk [H]

Synonym: Bind_2858

Alternate gene names: 182679770

Gene position: 3261626-3260193 (Counterclockwise)

Preceding gene: 182679771

Following gene: 182679769

Centisome position: 78.21

GC content: 65.97

Gene sequence:

>1434_bases
ATGCGCCGGTATCGCCGAGCCAAGATTGTGGCAACACTGGGTCCCGCGAGTTCCAGTCCGGAAACCATCCGGGCTCTGTT
TGACGCGGGCGCCGATGTGTTTCGGTTCAACTTCAGCCATGGCACCCATGAGGACCATCGGCAGCGCTATGAGATCGTGC
GCGCCATCGAGCAGGAAACCGGCCGGCCGATCTCGGTCCTCGCCGATCTGCAAGGGCCGAAACTGCGCATCGGCAAGCTG
GCCGCGGGGCCGATCATGCTAGAGGAAGGCACGACGATCACCTTTGATCTCGATCCGGAGCCCGGCACGCTCGCGCGGGT
GCCTCTGCCGCATCCGGAAGTCTTCGCGGCGCTTTCGCCTGGGGTCACGCTGCTGATCGATGATGGCAAGCTGCGCCTCA
CCATCGAGAAGGCCGAGCCGCAATCGGCCGTGGCGAAGGTGGTGACCGGGGGCCCGCTTTCCGAGCGCAAGGGCGTCAGC
ATCGTCGGGGCGGTTCTGCCGGTCTCGGCGCTGACCGAAAAGGATCGCAAGGATCTCGCTTTCGCGCTGGAGCTTGGCGC
CGACTGGATCGCTTTGTCCTTCGTGCAGCGGGCCGAAGATCTCGACGAGGTGCGCGCGCTGGCCAATCGCCCGATTGCGC
TGATGGCGAAGCTCGAAAAGCCTTCCGCCATCGACGGGCTCGAAGCCATTATCGCACGGTCCGATGCGGTCATGGTGGCG
CGCGGTGATCTCGGTGTCGAAATGGAGCCCGAGCGTGTGCCGATCGTGCAGCGGCGCATCGTGCATGCGGCGCGGGGGGC
GGGCAAGCCGGTGATCGTCGCCACGCAAATGCTGGAATCGATGATCACCACGCCGACGCCGACCCGCGCCGAGGCCTCCG
ATGTCGCGACCGCCATCTATGAGGGCGCCGATGCGGTGATGCTGTCCGCCGAGACGGCGTCTGGCCGCTATCCGATCGAG
GCCGTGACGATGATGAACAAGATCATCACGGCCGTGGAGCAGGACCCTGTGTATCGCGAAGGGCTTGATGCGACGGGGCC
GACGCCGCAGGCGAATGTCTCGGATGTGATCTGCAGCGCCTTGCATCGCTCGGCGGCAATCCTGCCTGTGGCGGCGCTGG
TCACCTATACGACCTCAGGCCTGACGAGCCTGCGCACCGCGCGGGAACGGCCGGCAGCGCCGATCCTGAGCCTGACGCCG
GAACTGGCGATCGCGCGGCGCCTGGCGCTGGTTTGGGGCACGCATCCGGTGCTCAGCCGTGCGCAGGCGCATGTGACGGA
CATCGTCGAGGATGCCTGCGCCGCGACGGAAAAGGAAGGGCTGGCGATCCCCGGCGATATTATCGCCATAGCCGGCGGCA
TGCCCTTTGGTGTGTCCGGCACGACCAATCTCCTGCGCATTGCACAAGTGCCAAGCGGTGCCGCCGCCAAATAA

Upstream 100 bases:

>100_bases
AATCACAAGTCGAAATTCATCAATACACGCTTCGTGTTCTAATGCGATTACGTAGCCATTCAATCACCTAGGGAGGCGGC
CCGCTGGGCCGATTGAGATC

Downstream 100 bases:

>100_bases
AGGATCGCTTTAGATCCCCACCGCGAACGAAACCCCATCAACCCCAAAAGCTCCCGCTTTTGGGGTTTTTGCTTGTGAAG
GTGAAAAGATCCCTCTCTTA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MRRYRRAKIVATLGPASSSPETIRALFDAGADVFRFNFSHGTHEDHRQRYEIVRAIEQETGRPISVLADLQGPKLRIGKL
AAGPIMLEEGTTITFDLDPEPGTLARVPLPHPEVFAALSPGVTLLIDDGKLRLTIEKAEPQSAVAKVVTGGPLSERKGVS
IVGAVLPVSALTEKDRKDLAFALELGADWIALSFVQRAEDLDEVRALANRPIALMAKLEKPSAIDGLEAIIARSDAVMVA
RGDLGVEMEPERVPIVQRRIVHAARGAGKPVIVATQMLESMITTPTPTRAEASDVATAIYEGADAVMLSAETASGRYPIE
AVTMMNKIITAVEQDPVYREGLDATGPTPQANVSDVICSALHRSAAILPVAALVTYTTSGLTSLRTARERPAAPILSLTP
ELAIARRLALVWGTHPVLSRAQAHVTDIVEDACAATEKEGLAIPGDIIAIAGGMPFGVSGTTNLLRIAQVPSGAAAK

Sequences:

>Translated_477_residues
MRRYRRAKIVATLGPASSSPETIRALFDAGADVFRFNFSHGTHEDHRQRYEIVRAIEQETGRPISVLADLQGPKLRIGKL
AAGPIMLEEGTTITFDLDPEPGTLARVPLPHPEVFAALSPGVTLLIDDGKLRLTIEKAEPQSAVAKVVTGGPLSERKGVS
IVGAVLPVSALTEKDRKDLAFALELGADWIALSFVQRAEDLDEVRALANRPIALMAKLEKPSAIDGLEAIIARSDAVMVA
RGDLGVEMEPERVPIVQRRIVHAARGAGKPVIVATQMLESMITTPTPTRAEASDVATAIYEGADAVMLSAETASGRYPIE
AVTMMNKIITAVEQDPVYREGLDATGPTPQANVSDVICSALHRSAAILPVAALVTYTTSGLTSLRTARERPAAPILSLTP
ELAIARRLALVWGTHPVLSRAQAHVTDIVEDACAATEKEGLAIPGDIIAIAGGMPFGVSGTTNLLRIAQVPSGAAAK
>Mature_477_residues
MRRYRRAKIVATLGPASSSPETIRALFDAGADVFRFNFSHGTHEDHRQRYEIVRAIEQETGRPISVLADLQGPKLRIGKL
AAGPIMLEEGTTITFDLDPEPGTLARVPLPHPEVFAALSPGVTLLIDDGKLRLTIEKAEPQSAVAKVVTGGPLSERKGVS
IVGAVLPVSALTEKDRKDLAFALELGADWIALSFVQRAEDLDEVRALANRPIALMAKLEKPSAIDGLEAIIARSDAVMVA
RGDLGVEMEPERVPIVQRRIVHAARGAGKPVIVATQMLESMITTPTPTRAEASDVATAIYEGADAVMLSAETASGRYPIE
AVTMMNKIITAVEQDPVYREGLDATGPTPQANVSDVICSALHRSAAILPVAALVTYTTSGLTSLRTARERPAAPILSLTP
ELAIARRLALVWGTHPVLSRAQAHVTDIVEDACAATEKEGLAIPGDIIAIAGGMPFGVSGTTNLLRIAQVPSGAAAK

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI32967597, Length=490, Percent_Identity=35.7142857142857, Blast_Score=275, Evalue=7e-74,
Organism=Homo sapiens, GI10835121, Length=490, Percent_Identity=35.7142857142857, Blast_Score=275, Evalue=8e-74,
Organism=Homo sapiens, GI33286422, Length=490, Percent_Identity=34.6938775510204, Blast_Score=273, Evalue=2e-73,
Organism=Homo sapiens, GI33286420, Length=490, Percent_Identity=34.6938775510204, Blast_Score=273, Evalue=2e-73,
Organism=Homo sapiens, GI33286418, Length=492, Percent_Identity=35.1626016260163, Blast_Score=268, Evalue=7e-72,
Organism=Homo sapiens, GI310128732, Length=287, Percent_Identity=33.4494773519164, Blast_Score=165, Evalue=8e-41,
Organism=Homo sapiens, GI310128730, Length=287, Percent_Identity=33.4494773519164, Blast_Score=165, Evalue=8e-41,
Organism=Homo sapiens, GI310128736, Length=235, Percent_Identity=31.063829787234, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI310128734, Length=235, Percent_Identity=31.063829787234, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=29.4392523364486, Blast_Score=105, Evalue=1e-22,
Organism=Escherichia coli, GI1788160, Length=488, Percent_Identity=38.9344262295082, Blast_Score=300, Evalue=1e-82,
Organism=Escherichia coli, GI1787965, Length=468, Percent_Identity=37.1794871794872, Blast_Score=288, Evalue=4e-79,
Organism=Caenorhabditis elegans, GI71984413, Length=482, Percent_Identity=37.1369294605809, Blast_Score=280, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI71984406, Length=482, Percent_Identity=37.1369294605809, Blast_Score=280, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI17506829, Length=482, Percent_Identity=37.1369294605809, Blast_Score=280, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI17506831, Length=482, Percent_Identity=37.1369294605809, Blast_Score=279, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI17544584, Length=488, Percent_Identity=34.0163934426229, Blast_Score=279, Evalue=3e-75,
Organism=Saccharomyces cerevisiae, GI6319279, Length=484, Percent_Identity=32.2314049586777, Blast_Score=234, Evalue=2e-62,
Organism=Saccharomyces cerevisiae, GI6324923, Length=491, Percent_Identity=32.7902240325866, Blast_Score=223, Evalue=4e-59,
Organism=Drosophila melanogaster, GI28571814, Length=492, Percent_Identity=33.739837398374, Blast_Score=259, Evalue=3e-69,
Organism=Drosophila melanogaster, GI24648964, Length=492, Percent_Identity=33.739837398374, Blast_Score=259, Evalue=3e-69,
Organism=Drosophila melanogaster, GI24648966, Length=406, Percent_Identity=35.9605911330049, Blast_Score=237, Evalue=1e-62,
Organism=Drosophila melanogaster, GI24581235, Length=492, Percent_Identity=30.2845528455285, Blast_Score=212, Evalue=5e-55,
Organism=Drosophila melanogaster, GI24646914, Length=287, Percent_Identity=36.2369337979094, Blast_Score=161, Evalue=7e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 50532; Mature: 50532

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRYRRAKIVATLGPASSSPETIRALFDAGADVFRFNFSHGTHEDHRQRYEIVRAIEQET
CCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHC
GRPISVLADLQGPKLRIGKLAAGPIMLEEGTTITFDLDPEPGTLARVPLPHPEVFAALSP
CCCEEEEECCCCCCEEECEEECCCEEEECCCEEEEEECCCCCCEEECCCCCHHHHHCCCC
GVTLLIDDGKLRLTIEKAEPQSAVAKVVTGGPLSERKGVSIVGAVLPVSALTEKDRKDLA
CEEEEEECCEEEEEEECCCCHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHCCHHHHE
FALELGADWIALSFVQRAEDLDEVRALANRPIALMAKLEKPSAIDGLEAIIARSDAVMVA
EEEHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEE
RGDLGVEMEPERVPIVQRRIVHAARGAGKPVIVATQMLESMITTPTPTRAEASDVATAIY
ECCCCCEECCCCCCHHHHHHHHHHCCCCCCEEHHHHHHHHHHCCCCCCCCCHHHHHHHHH
EGADAVMLSAETASGRYPIEAVTMMNKIITAVEQDPVYREGLDATGPTPQANVSDVICSA
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCHHHHHHHH
LHRSAAILPVAALVTYTTSGLTSLRTARERPAAPILSLTPELAIARRLALVWGTHPVLSR
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHCCCHHHHH
AQAHVTDIVEDACAATEKEGLAIPGDIIAIAGGMPFGVSGTTNLLRIAQVPSGAAAK
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCCC
>Mature Secondary Structure
MRRYRRAKIVATLGPASSSPETIRALFDAGADVFRFNFSHGTHEDHRQRYEIVRAIEQET
CCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHC
GRPISVLADLQGPKLRIGKLAAGPIMLEEGTTITFDLDPEPGTLARVPLPHPEVFAALSP
CCCEEEEECCCCCCEEECEEECCCEEEECCCEEEEEECCCCCCEEECCCCCHHHHHCCCC
GVTLLIDDGKLRLTIEKAEPQSAVAKVVTGGPLSERKGVSIVGAVLPVSALTEKDRKDLA
CEEEEEECCEEEEEEECCCCHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHCCHHHHE
FALELGADWIALSFVQRAEDLDEVRALANRPIALMAKLEKPSAIDGLEAIIARSDAVMVA
EEEHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEE
RGDLGVEMEPERVPIVQRRIVHAARGAGKPVIVATQMLESMITTPTPTRAEASDVATAIY
ECCCCCEECCCCCCHHHHHHHHHHCCCCCCEEHHHHHHHHHHCCCCCCCCCHHHHHHHHH
EGADAVMLSAETASGRYPIEAVTMMNKIITAVEQDPVYREGLDATGPTPQANVSDVICSA
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCHHHHHHHH
LHRSAAILPVAALVTYTTSGLTSLRTARERPAAPILSLTPELAIARRLALVWGTHPVLSR
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHCCCHHHHH
AQAHVTDIVEDACAATEKEGLAIPGDIIAIAGGMPFGVSGTTNLLRIAQVPSGAAAK
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA