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The map label for this gene is thcD [H]

Identifier: 182679438

GI number: 182679438

Start: 2840894

End: 2842099

Strand: Reverse

Name: thcD [H]

Synonym: Bind_2488

Alternate gene names: 182679438

Gene position: 2842099-2840894 (Counterclockwise)

Preceding gene: 182679439

Following gene: 182679437

Centisome position: 68.15

GC content: 57.71

Gene sequence:

>1206_bases
ATGGACGGTATCGTCATCATCGGTGCGGGGGAATGCGGCACACGAGCGGCATTCGCCCTGCGCGAAGCGGAATATTCTGG
ACCCGTAACACTGGTTGGAGCCGAGCCTCATCTTCCCTATGAACGGCCTCCGCTATCGAAGTCGATGAGCGGTGTGGTGC
AAATGAAGCTGATATGCGCGAGTGAAGCGCTTGAGGCTGCCGGTATCGACTATCTTCAGGGATTATCGGCCTCGAAGCTT
AATGCTGAAGCTGGATCTGTGATCCTGAGTGACGGGCGGGTGCTGAAGTATGAAAAGCTGCTTCTCGCCACGGGTGCACG
TCCAAGAAGACTAACATGTCCCGGTGCCGAACTTGCGCTTGACTTTCGTACTTATGCCGATGCCGAAGCGATTTTTTCCC
ATGCGGCAAAAGGTCGGCACGTTGCAATCATCGGCGGCGGTTTGATCGGGATGGAACTTGCGTCGGTCCTGCGTGGAAAG
AATATCAGCGTCAGTGTGATCGAAGCCGCGCCAAGACCTCTCGGACGTGCGGTGCCTGCGCGCTTGGCCGAAAAACTATA
TGCCAGACACATGGCTGAAGGCGTTGGCTTTCACCTGGATCGGGGCGTTGTGGCTATCAGCCACGATGGAGTGACTCTGA
CCGATGGTAGTATAGTGCCCGCTGATCTCGTTGTCAGTGCCATAGGTGTTTTGCCTGAAGTCGCTTTGGCGGAAGCGGCC
GGCCTGGCGACAGGCAACGGAATCCTGACAGATATGTATCTTCGCACCAGCACCCCAAACATTTTCGCTGCAGGCGATTG
TGCGGCGGTGATGCAACCCGGTGGAGGGCACATCCGGTATGAAGCTTGGCGAAATGCGAGAACCCAGGCCGAGACTGCCG
CGCGAAACATGGCTGGTGCTGCGGAGGCTTTCGCTGCCATCCCCTGGTTTTGGTCCGATCAATACGATCTTGGCCTCCAG
GTGGCGGGGCTGCCACAACCTGCCCATCAAAGTGTCTTGCGTTCGCTCATGACGGGCGAACTCGAATTTTATCTAGACGA
TGGGCGCCTGGTGGCTGTGGCAGGCCTGGGTGTCGGCAATAGCCTGGCCAAGGACATCAAGCTGGCTGAGATGTTGATTG
CCGCAGGGGCCAGCCCTGACCCTGTGGCATTGGCCGACCCCGGGACGAACCTTAAGACGTTTTTGAAAAACGCGCGGGCC
GCATGA

Upstream 100 bases:

>100_bases
CGTCGACAGGCAGGTGATGGTGCCTTTCGAACTCGTTACGCCCGAGAATATGGCAAAATACAACAATAAGAACTGACCCG
TCTTTTTGATTGGAGAGCAC

Downstream 100 bases:

>100_bases
TGCAGAAACTGCTAATCTTCCAGTCGCTCTGGGCCATGGAACGCCGGCATACGGATGGCCAGGAGCGCAGCCTCGACGAG
AACATCGCAATGATTTCGGC

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MDGIVIIGAGECGTRAAFALREAEYSGPVTLVGAEPHLPYERPPLSKSMSGVVQMKLICASEALEAAGIDYLQGLSASKL
NAEAGSVILSDGRVLKYEKLLLATGARPRRLTCPGAELALDFRTYADAEAIFSHAAKGRHVAIIGGGLIGMELASVLRGK
NISVSVIEAAPRPLGRAVPARLAEKLYARHMAEGVGFHLDRGVVAISHDGVTLTDGSIVPADLVVSAIGVLPEVALAEAA
GLATGNGILTDMYLRTSTPNIFAAGDCAAVMQPGGGHIRYEAWRNARTQAETAARNMAGAAEAFAAIPWFWSDQYDLGLQ
VAGLPQPAHQSVLRSLMTGELEFYLDDGRLVAVAGLGVGNSLAKDIKLAEMLIAAGASPDPVALADPGTNLKTFLKNARA
A

Sequences:

>Translated_401_residues
MDGIVIIGAGECGTRAAFALREAEYSGPVTLVGAEPHLPYERPPLSKSMSGVVQMKLICASEALEAAGIDYLQGLSASKL
NAEAGSVILSDGRVLKYEKLLLATGARPRRLTCPGAELALDFRTYADAEAIFSHAAKGRHVAIIGGGLIGMELASVLRGK
NISVSVIEAAPRPLGRAVPARLAEKLYARHMAEGVGFHLDRGVVAISHDGVTLTDGSIVPADLVVSAIGVLPEVALAEAA
GLATGNGILTDMYLRTSTPNIFAAGDCAAVMQPGGGHIRYEAWRNARTQAETAARNMAGAAEAFAAIPWFWSDQYDLGLQ
VAGLPQPAHQSVLRSLMTGELEFYLDDGRLVAVAGLGVGNSLAKDIKLAEMLIAAGASPDPVALADPGTNLKTFLKNARA
A
>Mature_401_residues
MDGIVIIGAGECGTRAAFALREAEYSGPVTLVGAEPHLPYERPPLSKSMSGVVQMKLICASEALEAAGIDYLQGLSASKL
NAEAGSVILSDGRVLKYEKLLLATGARPRRLTCPGAELALDFRTYADAEAIFSHAAKGRHVAIIGGGLIGMELASVLRGK
NISVSVIEAAPRPLGRAVPARLAEKLYARHMAEGVGFHLDRGVVAISHDGVTLTDGSIVPADLVVSAIGVLPEVALAEAA
GLATGNGILTDMYLRTSTPNIFAAGDCAAVMQPGGGHIRYEAWRNARTQAETAARNMAGAAEAFAAIPWFWSDQYDLGLQ
VAGLPQPAHQSVLRSLMTGELEFYLDDGRLVAVAGLGVGNSLAKDIKLAEMLIAAGASPDPVALADPGTNLKTFLKNARA
A

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=394, Percent_Identity=29.9492385786802, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI226437568, Length=389, Percent_Identity=30.0771208226221, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI65787454, Length=394, Percent_Identity=29.9492385786802, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI4757732, Length=340, Percent_Identity=29.1176470588235, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI22202629, Length=340, Percent_Identity=29.1176470588235, Blast_Score=112, Evalue=8e-25,
Organism=Escherichia coli, GI1788892, Length=402, Percent_Identity=33.5820895522388, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI1789065, Length=281, Percent_Identity=31.6725978647687, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1789765, Length=279, Percent_Identity=31.1827956989247, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1787352, Length=333, Percent_Identity=24.024024024024, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1789915, Length=185, Percent_Identity=30.2702702702703, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17559934, Length=383, Percent_Identity=27.9373368146214, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI32564386, Length=359, Percent_Identity=30.08356545961, Blast_Score=118, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24639257, Length=405, Percent_Identity=26.4197530864197, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI281359715, Length=405, Percent_Identity=26.4197530864197, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI281359713, Length=405, Percent_Identity=26.4197530864197, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24639250, Length=405, Percent_Identity=26.4197530864197, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI18543267, Length=405, Percent_Identity=26.4197530864197, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24639252, Length=405, Percent_Identity=26.4197530864197, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24585130, Length=403, Percent_Identity=23.5732009925558, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24581020, Length=357, Percent_Identity=26.0504201680672, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI28573993, Length=357, Percent_Identity=26.0504201680672, Blast_Score=96, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 41698; Mature: 41698

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGIVIIGAGECGTRAAFALREAEYSGPVTLVGAEPHLPYERPPLSKSMSGVVQMKLICA
CCCEEEEECCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHEEEEHHH
SEALEAAGIDYLQGLSASKLNAEAGSVILSDGRVLKYEKLLLATGARPRRLTCPGAELAL
HHHHHHCCCHHHHCCCCCCCCCCCCCEEEECCCEEEEEHHHHHCCCCCCEEECCCHHEEE
DFRTYADAEAIFSHAAKGRHVAIIGGGLIGMELASVLRGKNISVSVIEAAPRPLGRAVPA
EHHHHCCHHHHHHHHCCCCEEEEEECCHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHH
RLAEKLYARHMAEGVGFHLDRGVVAISHDGVTLTDGSIVPADLVVSAIGVLPEVALAEAA
HHHHHHHHHHHHHHCCEEECCCEEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHC
GLATGNGILTDMYLRTSTPNIFAAGDCAAVMQPGGGHIRYEAWRNARTQAETAARNMAGA
CCCCCCCEEEEEEEEECCCCEEECCCEEEEECCCCCEEEEHHHHCCHHHHHHHHHHHHHH
AEAFAAIPWFWSDQYDLGLQVAGLPQPAHQSVLRSLMTGELEFYLDDGRLVAVAGLGVGN
HHHHHHCCCEECCCCCCCEEEECCCCHHHHHHHHHHHCCCEEEEEECCCEEEEEECCCCH
SLAKDIKLAEMLIAAGASPDPVALADPGTNLKTFLKNARAA
HHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHCCCC
>Mature Secondary Structure
MDGIVIIGAGECGTRAAFALREAEYSGPVTLVGAEPHLPYERPPLSKSMSGVVQMKLICA
CCCEEEEECCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHEEEEHHH
SEALEAAGIDYLQGLSASKLNAEAGSVILSDGRVLKYEKLLLATGARPRRLTCPGAELAL
HHHHHHCCCHHHHCCCCCCCCCCCCCEEEECCCEEEEEHHHHHCCCCCCEEECCCHHEEE
DFRTYADAEAIFSHAAKGRHVAIIGGGLIGMELASVLRGKNISVSVIEAAPRPLGRAVPA
EHHHHCCHHHHHHHHCCCCEEEEEECCHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHH
RLAEKLYARHMAEGVGFHLDRGVVAISHDGVTLTDGSIVPADLVVSAIGVLPEVALAEAA
HHHHHHHHHHHHHHCCEEECCCEEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHC
GLATGNGILTDMYLRTSTPNIFAAGDCAAVMQPGGGHIRYEAWRNARTQAETAARNMAGA
CCCCCCCEEEEEEEEECCCCEEECCCEEEEECCCCCEEEEHHHHCCHHHHHHHHHHHHHH
AEAFAAIPWFWSDQYDLGLQVAGLPQPAHQSVLRSLMTGELEFYLDDGRLVAVAGLGVGN
HHHHHHCCCEECCCCCCCEEEECCCCHHHHHHHHHHHCCCEEEEEECCCEEEEEECCCCH
SLAKDIKLAEMLIAAGASPDPVALADPGTNLKTFLKNARAA
HHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]