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The map label for this gene is nuoB

Identifier: 182679352

GI number: 182679352

Start: 2739099

End: 2739641

Strand: Reverse

Name: nuoB

Synonym: Bind_2398

Alternate gene names: 182679352

Gene position: 2739641-2739099 (Counterclockwise)

Preceding gene: 182679353

Following gene: 182679351

Centisome position: 65.7

GC content: 58.56

Gene sequence:

>543_bases
ATGGGATTGATCGCCACACCTCAGCCGGTCGTCGGCACTCCCCCGGCGCAGGATAATTATTTCCTGTCGATCAACGACCA
GCTTGCCGACAAAGGGTTTCTCGTCACGTCGACGGATGAGCTCATCAATTGGGCGCGTACCGGCTCCTTGATGTGGATGA
CCTTTGGTCTTGCCTGCTGCGCCGTGGAAATGATGCAGATGTCGATGCCGCGTTATGATTGCGAGCGGTTCGGCTTTGCG
CCGCGCGGTTCGCCACGCCAATCCGATGTCATGATCGTCGCCGGCACGTTGACCAACAAGATGGCGCCCGCCTTGCGCAA
GGTCTACGACCAGATGCCCGAGCCGCGCTATGTCATTTCCATGGGCTCCTGCGCCAATGGTGGTGGCTATTACCATTATT
CCTATTCCGTCGTGCGCGGCTGCGATCGGATCGTGCCCGTCGATATTTACGTGCCGGGCTGTCCGCCTTCGGCGGAAGCG
CTGCTCTATGGTGTGCTTCTGCTGCAGAAAAAGATCCGCCGTACAGGCACGATCGAACGATAA

Upstream 100 bases:

>100_bases
CCTTCCGCACGATTGGGGATCTCGGCTTCTGGTCGATGATCATCTTCCTCGGAATCCTGACGGTCGGATTCATCTACGAA
TGGAAGAAAGGGGCGCTCGA

Downstream 100 bases:

>100_bases
GAGCCCATCTTGCGCCATGATCTTTCACCATGGCGCAAGATATCCTCGAGGAAGATGCGATGGATAATGATCGTCGGGAA
TTGCTGCATCAGGAATTGAC

Product: NADH-quinone oxidoreductase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B

Number of amino acids: Translated: 180; Mature: 179

Protein sequence:

>180_residues
MGLIATPQPVVGTPPAQDNYFLSINDQLADKGFLVTSTDELINWARTGSLMWMTFGLACCAVEMMQMSMPRYDCERFGFA
PRGSPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPSAEA
LLYGVLLLQKKIRRTGTIER

Sequences:

>Translated_180_residues
MGLIATPQPVVGTPPAQDNYFLSINDQLADKGFLVTSTDELINWARTGSLMWMTFGLACCAVEMMQMSMPRYDCERFGFA
PRGSPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPSAEA
LLYGVLLLQKKIRRTGTIER
>Mature_179_residues
GLIATPQPVVGTPPAQDNYFLSINDQLADKGFLVTSTDELINWARTGSLMWMTFGLACCAVEMMQMSMPRYDCERFGFAP
RGSPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPSAEAL
LYGVLLLQKKIRRTGTIER

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=147, Percent_Identity=78.2312925170068, Blast_Score=249, Evalue=1e-66,
Organism=Escherichia coli, GI1788624, Length=148, Percent_Identity=47.972972972973, Blast_Score=152, Evalue=1e-38,
Organism=Escherichia coli, GI1789074, Length=129, Percent_Identity=42.6356589147287, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1788834, Length=123, Percent_Identity=40.650406504065, Blast_Score=100, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17509685, Length=142, Percent_Identity=69.0140845070423, Blast_Score=221, Evalue=1e-58,
Organism=Drosophila melanogaster, GI18859983, Length=141, Percent_Identity=77.3049645390071, Blast_Score=243, Evalue=6e-65,
Organism=Drosophila melanogaster, GI24642371, Length=141, Percent_Identity=77.3049645390071, Blast_Score=243, Evalue=6e-65,
Organism=Drosophila melanogaster, GI24651058, Length=141, Percent_Identity=76.5957446808511, Blast_Score=237, Evalue=3e-63,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB_BEII9 (B2IHW6)

Other databases:

- EMBL:   CP001016
- RefSeq:   YP_001833498.1
- GeneID:   6200103
- GenomeReviews:   CP001016_GR
- KEGG:   bid:Bind_2398
- HOGENOM:   HBG553221
- OMA:   GKPIGSN
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 19906; Mature: 19775

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
6.1 %Met     (Translated Protein)
9.4 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
8.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLIATPQPVVGTPPAQDNYFLSINDQLADKGFLVTSTDELINWARTGSLMWMTFGLACC
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEECHHHHHHHHHCCCHHHHHHHHHHH
AVEMMQMSMPRYDCERFGFAPRGSPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVIS
HHHHHHHCCCCCCHHHCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHCCCCEEEEE
MGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPSAEALLYGVLLLQKKIRRTGTIER
ECCCCCCCCEEEEEEHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
GLIATPQPVVGTPPAQDNYFLSINDQLADKGFLVTSTDELINWARTGSLMWMTFGLACC
CCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEECHHHHHHHHHCCCHHHHHHHHHHH
AVEMMQMSMPRYDCERFGFAPRGSPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVIS
HHHHHHHCCCCCCHHHCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHCCCCEEEEE
MGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPSAEALLYGVLLLQKKIRRTGTIER
ECCCCCCCCEEEEEEHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA