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The map label for this gene is nuoI

Identifier: 182679344

GI number: 182679344

Start: 2731041

End: 2731529

Strand: Reverse

Name: nuoI

Synonym: Bind_2390

Alternate gene names: 182679344

Gene position: 2731529-2731041 (Counterclockwise)

Preceding gene: 182679345

Following gene: 182679343

Centisome position: 65.5

GC content: 59.1

Gene sequence:

>489_bases
ATGAGACTCGATTCTGCCGCTAAGGCGCTTTTTCTCAAGGAATTCGTGGGCGCTTTCGCGTTGTCCATGCGCTATTTCTT
CAAGCCGCGGCCGACGCTGAATTATCCGCACGAGAAGAATCCACAATCGCCACGGTATCGGGGCGAACATGCGCTACGCC
GTTATCCCAATGGCGAGGAACGCTGCATCGCCTGCAAGCTTTGCGAGGCGATCTGTCCGGCCAAGGCGATCACCATTGAG
GCGGGTCCCCGCCGCAATGACGGAACCCGCCGCACGACCCGCTACGATATCGACATGGTGAAATGCATCTATTGCGGTTT
CTGCCAGGAAGCCTGTCCGGTCGATGCGATCGTCGAGGGTCCGAACGCCGAATTCGCGGTCGAGACACGCGAGGAATTAT
ATTACGACAAGGATCGGCTCCTGCAGAACGGCGCCCGCTGGGAGCGCGAGATCGCACGGAATATCGCTCTCGACGCGCCC
TATCGCTGA

Upstream 100 bases:

>100_bases
CGCTACCGGCTGGGGCGCGGGCATGGGGCAATAGAGCCACGGAAAAGAACATGCGCCGCCACTTTCGGGTGTAGGGCACA
TGCTTCGGCAAGGAAACGAT

Downstream 100 bases:

>100_bases
GGAACCGATCATGGATGTTCAGGCGTTTTTCTTCACGCTCTTCTCGACCGTCATGATCGTGTCGGCCATTATGGTCATCG
CGGCGCGCAATCCGGTCCAT

Product: NADH dehydrogenase subunit I

Products: NA

Alternate protein names: NADH dehydrogenase I subunit I; NDH-1 subunit I

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MRLDSAAKALFLKEFVGAFALSMRYFFKPRPTLNYPHEKNPQSPRYRGEHALRRYPNGEERCIACKLCEAICPAKAITIE
AGPRRNDGTRRTTRYDIDMVKCIYCGFCQEACPVDAIVEGPNAEFAVETREELYYDKDRLLQNGARWEREIARNIALDAP
YR

Sequences:

>Translated_162_residues
MRLDSAAKALFLKEFVGAFALSMRYFFKPRPTLNYPHEKNPQSPRYRGEHALRRYPNGEERCIACKLCEAICPAKAITIE
AGPRRNDGTRRTTRYDIDMVKCIYCGFCQEACPVDAIVEGPNAEFAVETREELYYDKDRLLQNGARWEREIARNIALDAP
YR
>Mature_162_residues
MRLDSAAKALFLKEFVGAFALSMRYFFKPRPTLNYPHEKNPQSPRYRGEHALRRYPNGEERCIACKLCEAICPAKAITIE
AGPRRNDGTRRTTRYDIDMVKCIYCGFCQEACPVDAIVEGPNAEFAVETREELYYDKDRLLQNGARWEREIARNIALDAP
YR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Homo sapiens, GI4505371, Length=159, Percent_Identity=68.5534591194968, Blast_Score=228, Evalue=1e-60,
Organism=Escherichia coli, GI1788617, Length=142, Percent_Identity=38.0281690140845, Blast_Score=98, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17555194, Length=160, Percent_Identity=63.125, Blast_Score=211, Evalue=9e-56,
Organism=Drosophila melanogaster, GI17864306, Length=159, Percent_Identity=68.5534591194968, Blast_Score=230, Evalue=3e-61,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOI_BEII9 (B2IHV8)

Other databases:

- EMBL:   CP001016
- RefSeq:   YP_001833490.1
- GeneID:   6199402
- GenomeReviews:   CP001016_GR
- KEGG:   bid:Bind_2390
- HOGENOM:   HBG724359
- OMA:   NYPYEKG
- ProtClustDB:   PRK05888
- HAMAP:   MF_01351
- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR010226
- Gene3D:   G3DSA:1.10.1060.10
- TIGRFAMs:   TIGR01971

Pfam domain/function: PF00037 Fer4

EC number: =1.6.99.5

Molecular weight: Translated: 18732; Mature: 18732

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
4.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDSAAKALFLKEFVGAFALSMRYFFKPRPTLNYPHEKNPQSPRYRGEHALRRYPNGEE
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCH
RCIACKLCEAICPAKAITIEAGPRRNDGTRRTTRYDIDMVKCIYCGFCQEACPVDAIVEG
HHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCEEEC
PNAEFAVETREELYYDKDRLLQNGARWEREIARNIALDAPYR
CCCCEEEHHHHHHHHCHHHHHHCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRLDSAAKALFLKEFVGAFALSMRYFFKPRPTLNYPHEKNPQSPRYRGEHALRRYPNGEE
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCH
RCIACKLCEAICPAKAITIEAGPRRNDGTRRTTRYDIDMVKCIYCGFCQEACPVDAIVEG
HHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCEEEC
PNAEFAVETREELYYDKDRLLQNGARWEREIARNIALDAPYR
CCCCEEEHHHHHHHHCHHHHHHCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA