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The map label for this gene is thiG [H]

Identifier: 182679127

GI number: 182679127

Start: 2471224

End: 2472027

Strand: Reverse

Name: thiG [H]

Synonym: Bind_2164

Alternate gene names: 182679127

Gene position: 2472027-2471224 (Counterclockwise)

Preceding gene: 182679128

Following gene: 182679126

Centisome position: 59.28

GC content: 61.82

Gene sequence:

>804_bases
ATGAGCGATGGCAAGGAAACGGAGCCTTTTTCCGTCTATGGCGTTTCGCTCGCCTCGCGGCTGTTCGTTGGAACGGCGCA
ATATCCCTCGCCGGCCGTGCTCAGTCAGGCCTTGGAGGTCTCCGGCACGGAGGTCGTCACGGTTTCCTTGCGGCGGGAGT
CGGGAGCGAGCCGCGCGGGCGAGCATTTCTGGACACTCATCCGCGAACAAGGGCTGCGCGTGCTGCCGAATACCGCTGGC
TGCCGCACGGTGAAGGAAGCGGTTACGACCGCGCAAATGGCCCGCGAAGTGTTTGAGACATCCTGGATCAAGCTCGAAGT
CATTGGTGAAGACGATACCTTGCAGCCGGATGTCTTTGGTCTCGTCGAGGCCGCGGGCATTCTCTCGCGGGATGGTTTTC
AGGTCTTTCCCTATACGACCGATGATCTCGTCGTGGCCGAGAAACTGCTGGGCGCGGGCTGCGAGGTTTTGATGCCCTGG
GCCGCGCCGATCGGCTCCGGCCGTGGGCTTAATAATCCCTATGCTTTGCGCTCCTTGCGCGGACATTTCCCCAATGTGCC
CTTGATCGTTGATGCCGGTATCGGCGCGCCTTCCCATGCGGCGTCGGCGATGGAAATGGGCTATGACGCGATCCTGCTCA
ACACGGCCATTGCCAAAGCGGGTGATCCAGTCGCCATGGCGCGTGCCTTTGCCCTTGCCGTCGAGGCGGGACGTCTCGCT
TATCGGTCGCATCCCATGGAGCCACGCGACATGGCGACACCTTCCACCACGATGAGTGGCCTTGCCTTTCGAGATAGCGC
CTGA

Upstream 100 bases:

>100_bases
ATCAGCGCGCGACGACATCGTTGCAAGAAGGGGATAGCGTGGAAATCCTCATGCCGCGCCAAGGCGGCTAATCAAGACGG
ACGATTGGGAGGATCATATC

Downstream 100 bases:

>100_bases
TGCTCGATCCCTTTTATCTTATCGTCGATAATGCCGCCTGGCTCGCGCGATTGCTGCCCTGCGGCGTCAAGCTGGTGCAA
TTGCGGGTCAAGGACCTGAC

Product: thiazole biosynthesis family protein

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MSDGKETEPFSVYGVSLASRLFVGTAQYPSPAVLSQALEVSGTEVVTVSLRRESGASRAGEHFWTLIREQGLRVLPNTAG
CRTVKEAVTTAQMAREVFETSWIKLEVIGEDDTLQPDVFGLVEAAGILSRDGFQVFPYTTDDLVVAEKLLGAGCEVLMPW
AAPIGSGRGLNNPYALRSLRGHFPNVPLIVDAGIGAPSHAASAMEMGYDAILLNTAIAKAGDPVAMARAFALAVEAGRLA
YRSHPMEPRDMATPSTTMSGLAFRDSA

Sequences:

>Translated_267_residues
MSDGKETEPFSVYGVSLASRLFVGTAQYPSPAVLSQALEVSGTEVVTVSLRRESGASRAGEHFWTLIREQGLRVLPNTAG
CRTVKEAVTTAQMAREVFETSWIKLEVIGEDDTLQPDVFGLVEAAGILSRDGFQVFPYTTDDLVVAEKLLGAGCEVLMPW
AAPIGSGRGLNNPYALRSLRGHFPNVPLIVDAGIGAPSHAASAMEMGYDAILLNTAIAKAGDPVAMARAFALAVEAGRLA
YRSHPMEPRDMATPSTTMSGLAFRDSA
>Mature_266_residues
SDGKETEPFSVYGVSLASRLFVGTAQYPSPAVLSQALEVSGTEVVTVSLRRESGASRAGEHFWTLIREQGLRVLPNTAGC
RTVKEAVTTAQMAREVFETSWIKLEVIGEDDTLQPDVFGLVEAAGILSRDGFQVFPYTTDDLVVAEKLLGAGCEVLMPWA
APIGSGRGLNNPYALRSLRGHFPNVPLIVDAGIGAPSHAASAMEMGYDAILLNTAIAKAGDPVAMARAFALAVEAGRLAY
RSHPMEPRDMATPSTTMSGLAFRDSA

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family [H]

Homologues:

Organism=Escherichia coli, GI48994993, Length=243, Percent_Identity=42.3868312757202, Blast_Score=180, Evalue=8e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR008867 [H]

Pfam domain/function: PF05690 ThiG [H]

EC number: NA

Molecular weight: Translated: 28374; Mature: 28242

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDGKETEPFSVYGVSLASRLFVGTAQYPSPAVLSQALEVSGTEVVTVSLRRESGASRAG
CCCCCCCCCEEEEHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCHHH
EHFWTLIREQGLRVLPNTAGCRTVKEAVTTAQMAREVFETSWIKLEVIGEDDTLQPDVFG
HHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHH
LVEAAGILSRDGFQVFPYTTDDLVVAEKLLGAGCEVLMPWAAPIGSGRGLNNPYALRSLR
HHHHHHHHCCCCCEEECCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHH
GHFPNVPLIVDAGIGAPSHAASAMEMGYDAILLNTAIAKAGDPVAMARAFALAVEAGRLA
CCCCCCCEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
YRSHPMEPRDMATPSTTMSGLAFRDSA
HHCCCCCCHHCCCCCCHHCCEEECCCC
>Mature Secondary Structure 
SDGKETEPFSVYGVSLASRLFVGTAQYPSPAVLSQALEVSGTEVVTVSLRRESGASRAG
CCCCCCCCEEEEHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCHHH
EHFWTLIREQGLRVLPNTAGCRTVKEAVTTAQMAREVFETSWIKLEVIGEDDTLQPDVFG
HHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHH
LVEAAGILSRDGFQVFPYTTDDLVVAEKLLGAGCEVLMPWAAPIGSGRGLNNPYALRSLR
HHHHHHHHCCCCCEEECCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHH
GHFPNVPLIVDAGIGAPSHAASAMEMGYDAILLNTAIAKAGDPVAMARAFALAVEAGRLA
CCCCCCCEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
YRSHPMEPRDMATPSTTMSGLAFRDSA
HHCCCCCCHHCCCCCCHHCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA