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The map label for this gene is thiE [H]

Identifier: 182679126

GI number: 182679126

Start: 2470619

End: 2471224

Strand: Reverse

Name: thiE [H]

Synonym: Bind_2163

Alternate gene names: 182679126

Gene position: 2471224-2470619 (Counterclockwise)

Preceding gene: 182679127

Following gene: 182679122

Centisome position: 59.26

GC content: 60.73

Gene sequence:

>606_bases
ATGCTCGATCCCTTTTATCTTATCGTCGATAATGCCGCCTGGCTCGCGCGATTGCTGCCCTGCGGCGTCAAGCTGGTGCA
ATTGCGGGTCAAGGACCTGACCGGCGAGCCTTTGCGCGCGGAAATCGCCAAGGCCAGAACCTTGTGCGATACGCATGGGG
CCCAATTGATCGTCAATGATTATTGGGTCGAGGCGATCGATCTCGGCTGCGATTATGTGCATCTCGGCCAGGGCGATCTC
GACACCGCCGATCTCCCCGCGATCCGCCGCGCGGGACTGCGGCTCGGCCTCTCCACACATGATGAAAAAGAGCTGGAACG
GGCCTTGGCCTGCGCGCCGGATTATATCGCGCTCGGGCCGATCTATCCGACGATTTTGAAAGCCATGCCTTTCGCGCCGC
AAGGCTTGGCGCGTCTCGGTGAATGGAAACGCAAGATCGGCTCCATTCCCCTCGTCGGCATCGGTGGCATCAATCTCGAC
CGCGTGGCGGGCGTGCTGGAAGCCGGTGCGGATAGTGCCTCTGTTGTGACCGATATCAGCCTAAATCCGGATCCTGAACA
AAGGACACGCGATTGGCTGGCCGCGACACGCCGCTTCGTGGCGTGA

Upstream 100 bases:

>100_bases
AGGCGGGACGTCTCGCTTATCGGTCGCATCCCATGGAGCCACGCGACATGGCGACACCTTCCACCACGATGAGTGGCCTT
GCCTTTCGAGATAGCGCCTG

Downstream 100 bases:

>100_bases
GCACTCGACGTCTTTACGAAGATACGTTTAATTATCGATATCCTGAAAGAGCTTTCCAGGAAGATAATATTCATTCAACC
ATGCTAACTTGATCACTTCT

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MLDPFYLIVDNAAWLARLLPCGVKLVQLRVKDLTGEPLRAEIAKARTLCDTHGAQLIVNDYWVEAIDLGCDYVHLGQGDL
DTADLPAIRRAGLRLGLSTHDEKELERALACAPDYIALGPIYPTILKAMPFAPQGLARLGEWKRKIGSIPLVGIGGINLD
RVAGVLEAGADSASVVTDISLNPDPEQRTRDWLAATRRFVA

Sequences:

>Translated_201_residues
MLDPFYLIVDNAAWLARLLPCGVKLVQLRVKDLTGEPLRAEIAKARTLCDTHGAQLIVNDYWVEAIDLGCDYVHLGQGDL
DTADLPAIRRAGLRLGLSTHDEKELERALACAPDYIALGPIYPTILKAMPFAPQGLARLGEWKRKIGSIPLVGIGGINLD
RVAGVLEAGADSASVVTDISLNPDPEQRTRDWLAATRRFVA
>Mature_201_residues
MLDPFYLIVDNAAWLARLLPCGVKLVQLRVKDLTGEPLRAEIAKARTLCDTHGAQLIVNDYWVEAIDLGCDYVHLGQGDL
DTADLPAIRRAGLRLGLSTHDEKELERALACAPDYIALGPIYPTILKAMPFAPQGLARLGEWKRKIGSIPLVGIGGINLD
RVAGVLEAGADSASVVTDISLNPDPEQRTRDWLAATRRFVA

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=196, Percent_Identity=44.3877551020408, Blast_Score=158, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6325042, Length=208, Percent_Identity=33.1730769230769, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 21862; Mature: 21862

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDPFYLIVDNAAWLARLLPCGVKLVQLRVKDLTGEPLRAEIAKARTLCDTHGAQLIVND
CCCCEEEEECCHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEH
YWVEAIDLGCDYVHLGQGDLDTADLPAIRRAGLRLGLSTHDEKELERALACAPDYIALGP
HHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEECC
IYPTILKAMPFAPQGLARLGEWKRKIGSIPLVGIGGINLDRVAGVLEAGADSASVVTDIS
HHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHCCCCCCEEEEEEC
LNPDPEQRTRDWLAATRRFVA
CCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLDPFYLIVDNAAWLARLLPCGVKLVQLRVKDLTGEPLRAEIAKARTLCDTHGAQLIVND
CCCCEEEEECCHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEH
YWVEAIDLGCDYVHLGQGDLDTADLPAIRRAGLRLGLSTHDEKELERALACAPDYIALGP
HHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEECC
IYPTILKAMPFAPQGLARLGEWKRKIGSIPLVGIGGINLDRVAGVLEAGADSASVVTDIS
HHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHCCCCCCEEEEEEC
LNPDPEQRTRDWLAATRRFVA
CCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371431 [H]