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The map label for this gene is dhaAF [H]

Identifier: 182679052

GI number: 182679052

Start: 2387488

End: 2388381

Strand: Reverse

Name: dhaAF [H]

Synonym: Bind_2088

Alternate gene names: 182679052

Gene position: 2388381-2387488 (Counterclockwise)

Preceding gene: 182679059

Following gene: 182679047

Centisome position: 57.27

GC content: 55.03

Gene sequence:

>894_bases
ATGAATATGAATATCGCAGCCATTCCGAACTCTGTGGAAATCTCCTTGCGCACAGCCAAGGTCGGTCCTGTGACGGTCTT
CTACCGCGAAGCTGGCGATGCAGCGAACCCGACGCTTCTGCTGTTACATGGCTTTCCGACTTCCTCGCATATGTTCCGGG
ATCTGATCCCGCTTTTGGCTGATCGTTATCATATTATCGCGCCTGATATGCCGGGTTTTGGTTTCACCACGGCGCCCGAA
CGGGGTCGGTTCGACTATACATTCGACAATTTGACAAAGGTCATCCAGGATTTTACCGATGTCATCCGCCTTGAGCATTA
TGCGATCTATGTCTTCGATTACGGGGCGCCCGTCGGTTATCGTCTGGCCATGGCGCGGCCCGAGCGCGTGTCAGCGATTA
TCTCGCAGAACGGCAATGCTTATGAAGAAGGTTTGAGCGAGGGCTGGACGCCAATCCAGAACTATTGGCGTGAGCCAAGC
CAGGAGAACCGGGACGCTATTCGCTCGCTGCTGACGCTCGAGGGCACCAAATGGCAATATGTGCATGGTGTCGCCGATGA
GATGAAAGTAGCACCCGAAGCCTATTGGCTCGATTATGCTTTGCTATCGCGCCCAGGCAATGACGAGATCCAGCTCGACC
TCTTTGGCAATTATGCAACGAATGTCGCGCTCTATCCCAAGTTTCATGCCTATTTCGCGGCCCAGCAGCCGCCATTCCTC
GCGGTCTGGGGCAAGAATGATCCGTTCTTCCTGCCGCCAGGCGCCGAAGCCTTCAAGCGAGACAATCCAAAAGCCGAAGT
GCATTTCTTCGACACCGGTCATTTCGCACTTGAGACGCATGCGCGTGAGATCGCCAGCACGATACGGGCGTTTTTGGGCC
GCGTGACTGCGTGA

Upstream 100 bases:

>100_bases
GACTGCCGAACTCTAAATATTCTGCCGGAATACGCAAGGCTATATCACAAGAAGGCATGATGATACGCGTCGATGAGACG
TGATAATCCGGAGAGACGCT

Downstream 100 bases:

>100_bases
AACCGAGACTTGATTCCCGTCATTGGTCGAGGATTGGCATGATCACCAACCCTCTTGCCATGTTGCATGGATGCGTCAGG
ATCATTGCATCAGTTCAAGC

Product: alpha/beta hydrolase fold protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MNMNIAAIPNSVEISLRTAKVGPVTVFYREAGDAANPTLLLLHGFPTSSHMFRDLIPLLADRYHIIAPDMPGFGFTTAPE
RGRFDYTFDNLTKVIQDFTDVIRLEHYAIYVFDYGAPVGYRLAMARPERVSAIISQNGNAYEEGLSEGWTPIQNYWREPS
QENRDAIRSLLTLEGTKWQYVHGVADEMKVAPEAYWLDYALLSRPGNDEIQLDLFGNYATNVALYPKFHAYFAAQQPPFL
AVWGKNDPFFLPPGAEAFKRDNPKAEVHFFDTGHFALETHAREIASTIRAFLGRVTA

Sequences:

>Translated_297_residues
MNMNIAAIPNSVEISLRTAKVGPVTVFYREAGDAANPTLLLLHGFPTSSHMFRDLIPLLADRYHIIAPDMPGFGFTTAPE
RGRFDYTFDNLTKVIQDFTDVIRLEHYAIYVFDYGAPVGYRLAMARPERVSAIISQNGNAYEEGLSEGWTPIQNYWREPS
QENRDAIRSLLTLEGTKWQYVHGVADEMKVAPEAYWLDYALLSRPGNDEIQLDLFGNYATNVALYPKFHAYFAAQQPPFL
AVWGKNDPFFLPPGAEAFKRDNPKAEVHFFDTGHFALETHAREIASTIRAFLGRVTA
>Mature_297_residues
MNMNIAAIPNSVEISLRTAKVGPVTVFYREAGDAANPTLLLLHGFPTSSHMFRDLIPLLADRYHIIAPDMPGFGFTTAPE
RGRFDYTFDNLTKVIQDFTDVIRLEHYAIYVFDYGAPVGYRLAMARPERVSAIISQNGNAYEEGLSEGWTPIQNYWREPS
QENRDAIRSLLTLEGTKWQYVHGVADEMKVAPEAYWLDYALLSRPGNDEIQLDLFGNYATNVALYPKFHAYFAAQQPPFL
AVWGKNDPFFLPPGAEAFKRDNPKAEVHFFDTGHFALETHAREIASTIRAFLGRVTA

Specific function: Catalyzes hydrolytic cleavage of carbon-halogen bonds in halogenated aliphatic compounds, leading to the formation of the corresponding primary alcohols, halide ions and protons. Has a broad substrate specificity, which includes mono- and di- chlorinated

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the haloalkane dehalogenase family. Type 2 subfamily [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6324392, Length=270, Percent_Identity=50.3703703703704, Blast_Score=264, Evalue=2e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.8.1.5 [H]

Molecular weight: Translated: 33544; Mature: 33544

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMNIAAIPNSVEISLRTAKVGPVTVFYREAGDAANPTLLLLHGFPTSSHMFRDLIPLLA
CCCEEEECCCCEEEEEEEECCCCEEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHHH
DRYHIIAPDMPGFGFTTAPERGRFDYTFDNLTKVIQDFTDVIRLEHYAIYVFDYGAPVGY
CCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCE
RLAMARPERVSAIISQNGNAYEEGLSEGWTPIQNYWREPSQENRDAIRSLLTLEGTKWQY
EEEECCCHHHHHHHHCCCCHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHHHCCCCCEEE
VHGVADEMKVAPEAYWLDYALLSRPGNDEIQLDLFGNYATNVALYPKFHAYFAAQQPPFL
EECCHHHHHCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEECCCHHEEEECCCCCEE
AVWGKNDPFFLPPGAEAFKRDNPKAEVHFFDTGHFALETHAREIASTIRAFLGRVTA
EEECCCCCEEECCCHHHHHCCCCCEEEEEEECCCEEEHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNMNIAAIPNSVEISLRTAKVGPVTVFYREAGDAANPTLLLLHGFPTSSHMFRDLIPLLA
CCCEEEECCCCEEEEEEEECCCCEEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHHH
DRYHIIAPDMPGFGFTTAPERGRFDYTFDNLTKVIQDFTDVIRLEHYAIYVFDYGAPVGY
CCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCE
RLAMARPERVSAIISQNGNAYEEGLSEGWTPIQNYWREPSQENRDAIRSLLTLEGTKWQY
EEEECCCHHHHHHHHCCCCHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHHHCCCCCEEE
VHGVADEMKVAPEAYWLDYALLSRPGNDEIQLDLFGNYATNVALYPKFHAYFAAQQPPFL
EECCHHHHHCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEECCCHHEEEECCCCCEE
AVWGKNDPFFLPPGAEAFKRDNPKAEVHFFDTGHFALETHAREIASTIRAFLGRVTA
EEECCCCCEEECCCHHHHHCCCCCEEEEEEECCCEEEHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10094681; 10735862 [H]