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The map label for this gene is hyi [C]

Identifier: 182679050

GI number: 182679050

Start: 2385323

End: 2386132

Strand: Direct

Name: hyi [C]

Synonym: Bind_2086

Alternate gene names: 182679050

Gene position: 2385323-2386132 (Clockwise)

Preceding gene: 182679049

Following gene: 182679051

Centisome position: 57.2

GC content: 58.52

Gene sequence:

>810_bases
ATGCCACGTTTTGCCGCCAATCTCACGATGATGTTCAATGAATGGGATTTTCTCGATCGTTTCGATGCCGCCGCCGACGC
CGGTTTCGAGGCTGTCGAATATTTGTTTCCCTATGATTTCACGCCGGAGGCGATTGCCGAGCGTTTGACACGCAATGGTC
TGACCCAGGCCCTGTTCAATCTGCCGCCGGGCGATTGGGCCGCCGGCGAACGCGGTCTTGCCGCCCTACCCGGCCGCTTC
GAGGATGTGAAGGCGGCCGTGACACACGCTCTCCCCTATGCGGAGGCGACGGGTGTCAAGCGGCTGCATCTGATGGCGGG
CATCGGCGAGCGATCGAGTGCGGCAGCGACCGCCGCCTATTCTCAATCCGTTGCCTGGACGGCGGAAATTTTGGCCGACA
AAGGCCTCGACCTTCTGCTCGAACCGATCAACAGCCGTGACATGCCAGGCTATTTCCTGAATGATTTTGCCTGGACGGAT
GCCTTCATCAAAGAGCTCGCCGCGCCCAATCTAAAACTACAATTCGACATTTATCATCGTCAGATCATGCATGGCGATGT
GACCATGGCTTTCCGCCAGCTCCTGCCTTTCATCGGTCACGTGCAGATCGCAAGCGTCCCCTCGCGCCATGAACCGGATG
GCGAGGAATTGAACTATGGTTTTCTTTTCGAGGAGATGGACCGGCTGGGCTATGCCGGTTTCACGGGCTGCGAATACCGC
CCGCGCGCCGGCACCTTGGCGGGACTCGATTGGTTCAAACCCTATGCGCGGAGAGAACCCGTGTTGAGCTTGACGGGAGA
ACGGTCATGA

Upstream 100 bases:

>100_bases
TGCGTTAAGACAACAAGATAAGGGCGTTTTCAGATTCGGATGAATCTGGAAATACACCGATAAGCCATGCGATCACCCTG
AACGTGTAGAGGATGCCGCC

Downstream 100 bases:

>100_bases
GCTTGCTGCTCGGTTGCATCGCCGATGATTATACAGGCGCGTCCGATCTCGCAAATACACTTGCCCGCAATGGATTGCGG
ACGATCCAGACCATCGGCCT

Product: hydroxypyruvate isomerase

Products: 2-hydroxy-3-oxopropanoate

Alternate protein names: ORF4 [H]

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MPRFAANLTMMFNEWDFLDRFDAAADAGFEAVEYLFPYDFTPEAIAERLTRNGLTQALFNLPPGDWAAGERGLAALPGRF
EDVKAAVTHALPYAEATGVKRLHLMAGIGERSSAAATAAYSQSVAWTAEILADKGLDLLLEPINSRDMPGYFLNDFAWTD
AFIKELAAPNLKLQFDIYHRQIMHGDVTMAFRQLLPFIGHVQIASVPSRHEPDGEELNYGFLFEEMDRLGYAGFTGCEYR
PRAGTLAGLDWFKPYARREPVLSLTGERS

Sequences:

>Translated_269_residues
MPRFAANLTMMFNEWDFLDRFDAAADAGFEAVEYLFPYDFTPEAIAERLTRNGLTQALFNLPPGDWAAGERGLAALPGRF
EDVKAAVTHALPYAEATGVKRLHLMAGIGERSSAAATAAYSQSVAWTAEILADKGLDLLLEPINSRDMPGYFLNDFAWTD
AFIKELAAPNLKLQFDIYHRQIMHGDVTMAFRQLLPFIGHVQIASVPSRHEPDGEELNYGFLFEEMDRLGYAGFTGCEYR
PRAGTLAGLDWFKPYARREPVLSLTGERS
>Mature_268_residues
PRFAANLTMMFNEWDFLDRFDAAADAGFEAVEYLFPYDFTPEAIAERLTRNGLTQALFNLPPGDWAAGERGLAALPGRFE
DVKAAVTHALPYAEATGVKRLHLMAGIGERSSAAATAAYSQSVAWTAEILADKGLDLLLEPINSRDMPGYFLNDFAWTDA
FIKELAAPNLKLQFDIYHRQIMHGDVTMAFRQLLPFIGHVQIASVPSRHEPDGEELNYGFLFEEMDRLGYAGFTGCEYRP
RAGTLAGLDWFKPYARREPVLSLTGERS

Specific function: Catalyzes The Reversible Isomerization Between Hydroxypyruvate And 2-Hydroxy-3-Oxopropanoate (Also Termed Tartronate Semialdehyde). [C]

COG id: COG3622

COG function: function code G; Hydroxypyruvate isomerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hyi family [H]

Homologues:

Organism=Homo sapiens, GI300068962, Length=265, Percent_Identity=43.7735849056604, Blast_Score=200, Evalue=1e-51,
Organism=Homo sapiens, GI300068964, Length=243, Percent_Identity=43.2098765432099, Blast_Score=174, Evalue=1e-43,
Organism=Escherichia coli, GI1786718, Length=257, Percent_Identity=43.579766536965, Blast_Score=224, Evalue=6e-60,
Organism=Escherichia coli, GI1789095, Length=257, Percent_Identity=42.8015564202335, Blast_Score=204, Evalue=5e-54,
Organism=Caenorhabditis elegans, GI17552022, Length=240, Percent_Identity=37.0833333333333, Blast_Score=162, Evalue=2e-40,
Organism=Drosophila melanogaster, GI17530883, Length=256, Percent_Identity=33.203125, Blast_Score=149, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013022
- InterPro:   IPR012307 [H]

Pfam domain/function: PF01261 AP_endonuc_2 [H]

EC number: 5.3.1.22

Molecular weight: Translated: 30010; Mature: 29879

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRFAANLTMMFNEWDFLDRFDAAADAGFEAVEYLFPYDFTPEAIAERLTRNGLTQALFN
CCCCCCCEEEEECCCCHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHC
LPPGDWAAGERGLAALPGRFEDVKAAVTHALPYAEATGVKRLHLMAGIGERSSAAATAAY
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHCCCHHHHHHHCCCCCCHHHHHHHH
SQSVAWTAEILADKGLDLLLEPINSRDMPGYFLNDFAWTDAFIKELAAPNLKLQFDIYHR
HHHHHHHHHHHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHH
QIMHGDVTMAFRQLLPFIGHVQIASVPSRHEPDGEELNYGFLFEEMDRLGYAGFTGCEYR
HHHCCHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCC
PRAGTLAGLDWFKPYARREPVLSLTGERS
CCCCCCCCHHHHHHHHHCCCEEECCCCCC
>Mature Secondary Structure 
PRFAANLTMMFNEWDFLDRFDAAADAGFEAVEYLFPYDFTPEAIAERLTRNGLTQALFN
CCCCCCEEEEECCCCHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHC
LPPGDWAAGERGLAALPGRFEDVKAAVTHALPYAEATGVKRLHLMAGIGERSSAAATAAY
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHCCCHHHHHHHCCCCCCHHHHHHHH
SQSVAWTAEILADKGLDLLLEPINSRDMPGYFLNDFAWTDAFIKELAAPNLKLQFDIYHR
HHHHHHHHHHHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHH
QIMHGDVTMAFRQLLPFIGHVQIASVPSRHEPDGEELNYGFLFEEMDRLGYAGFTGCEYR
HHHCCHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCC
PRAGTLAGLDWFKPYARREPVLSLTGERS
CCCCCCCCHHHHHHHHHCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 10 {hydroxypyruvate}} [C]

Substrates: hydroxypyruvate

Specific reaction: hydroxypyruvate = 2-hydroxy-3-oxopropanoate; Other Name(s): hydroxypyruvate ketol-isomerase

General reaction: Intramolecular oxidoreduction; Isomerization [C]

Inhibitor: Cu2+; Fe2+; Hydroxy l amine; Iodoacetate; NaCN [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7851418 [H]