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The map label for this gene is ygbJ [H]

Identifier: 182679049

GI number: 182679049

Start: 2384261

End: 2385187

Strand: Direct

Name: ygbJ [H]

Synonym: Bind_2085

Alternate gene names: 182679049

Gene position: 2384261-2385187 (Clockwise)

Preceding gene: 182679048

Following gene: 182679050

Centisome position: 57.17

GC content: 60.19

Gene sequence:

>927_bases
ATGAATGCGGTCACAAATGCCACGGAATTCCGGCTCGGGCGCGTCGCCGTCATCGGACTGGGCTCCATGGGTTATGGCAT
GGCGCAATCGTTAAAGCGCGCCGGTTTCGATGTCACCGGTTGCGACACCAATCAGGACGCCGTTGCTCGCTTTGTTGCGG
AGGGGGGACGCAGCAGCCCCATACTGGCGGACGTAGCGGCGGACGCCGATTGCGTTATCTCGGTCGTGGTCAATGCGGCG
CAGACGGAAGCTATCCTCTTCGGCGATGGTGGCGTGGCCGAGACCATGCCCAAGGACAGCGTGTTCATCTCCTGCGCCAC
CATGGATCCCGATATTGTTCGCCGTCTCGCTGCCAAGCTCGAAGCGACGGGCCGACATTATCTCGATGCACCAATCAGCG
GCGGCGCAAAGCGCGCGGCGGAAGGCGCCTTGACCATCCTCGCCTCCGGTTCGCCAAAAGCTTTTGCAAAGGCGCGCCCG
GCACTCAATGCCATGGCTGCCAAACTCTATGAACTCGGCGATGCGCCAGGCCAAGGCGCCGCGTTCAAAATGATCAACCA
GCTTCTCGCCGGCGTACACATTGCCGTCGCCAGCGAGGCAATCACCTTTGCCGCCCGTCAAGGTCTCGACCTCGACAAGG
TTTATGAGGTTATCACCGCTTCGGCAGGCAATTCCTGGATGTTCGAGAATCGCGTGCCACATGTCCTCGAAGGCGATTAC
ACGCCGCGCAGCGCCGTGGATATTTTCGTCAAGGATCTCGGCATTATTCAGGATATGGCACGCAACACCAAATTTCCCGT
GCCGATCGCCGCCGCCGCATTGCAAATGTTTCTGATGACCTCCGCCGCCGGCATGGGCCGCGAGGATGATGCCTCAGTCG
CACGGCTTTATGCCAACATCACCGGGACGAAACTGCCTGGAGCATAA

Upstream 100 bases:

>100_bases
AATCACTGAGACCAAGGGTGGATCCGGCTCTGAATCCACCCGATCTCAGACATTTTCACCAATCATTTCACGGCCTCTTC
GACAATGAAGGAAAAATAGA

Downstream 100 bases:

>100_bases
CCCCCGAAAGCAGAACGCTTTCTGGATCACGATTATGCGTTAAGACAACAAGATAAGGGCGTTTTCAGATTCGGATGAAT
CTGGAAATACACCGATAAGC

Product: 3-hydroxyisobutyrate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MNAVTNATEFRLGRVAVIGLGSMGYGMAQSLKRAGFDVTGCDTNQDAVARFVAEGGRSSPILADVAADADCVISVVVNAA
QTEAILFGDGGVAETMPKDSVFISCATMDPDIVRRLAAKLEATGRHYLDAPISGGAKRAAEGALTILASGSPKAFAKARP
ALNAMAAKLYELGDAPGQGAAFKMINQLLAGVHIAVASEAITFAARQGLDLDKVYEVITASAGNSWMFENRVPHVLEGDY
TPRSAVDIFVKDLGIIQDMARNTKFPVPIAAAALQMFLMTSAAGMGREDDASVARLYANITGTKLPGA

Sequences:

>Translated_308_residues
MNAVTNATEFRLGRVAVIGLGSMGYGMAQSLKRAGFDVTGCDTNQDAVARFVAEGGRSSPILADVAADADCVISVVVNAA
QTEAILFGDGGVAETMPKDSVFISCATMDPDIVRRLAAKLEATGRHYLDAPISGGAKRAAEGALTILASGSPKAFAKARP
ALNAMAAKLYELGDAPGQGAAFKMINQLLAGVHIAVASEAITFAARQGLDLDKVYEVITASAGNSWMFENRVPHVLEGDY
TPRSAVDIFVKDLGIIQDMARNTKFPVPIAAAALQMFLMTSAAGMGREDDASVARLYANITGTKLPGA
>Mature_308_residues
MNAVTNATEFRLGRVAVIGLGSMGYGMAQSLKRAGFDVTGCDTNQDAVARFVAEGGRSSPILADVAADADCVISVVVNAA
QTEAILFGDGGVAETMPKDSVFISCATMDPDIVRRLAAKLEATGRHYLDAPISGGAKRAAEGALTILASGSPKAFAKARP
ALNAMAAKLYELGDAPGQGAAFKMINQLLAGVHIAVASEAITFAARQGLDLDKVYEVITASAGNSWMFENRVPHVLEGDY
TPRSAVDIFVKDLGIIQDMARNTKFPVPIAAAALQMFLMTSAAGMGREDDASVARLYANITGTKLPGA

Specific function: Unknown

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=288, Percent_Identity=32.9861111111111, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI40556376, Length=284, Percent_Identity=24.6478873239437, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1789092, Length=303, Percent_Identity=47.8547854785479, Blast_Score=253, Evalue=1e-68,
Organism=Escherichia coli, GI145693186, Length=287, Percent_Identity=29.2682926829268, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1790315, Length=280, Percent_Identity=32.1428571428571, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1786719, Length=292, Percent_Identity=25.6849315068493, Blast_Score=109, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17557316, Length=268, Percent_Identity=29.4776119402985, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24655230, Length=290, Percent_Identity=30.3448275862069, Blast_Score=137, Evalue=8e-33,
Organism=Drosophila melanogaster, GI19922568, Length=290, Percent_Identity=30.3448275862069, Blast_Score=137, Evalue=8e-33,
Organism=Drosophila melanogaster, GI28574115, Length=277, Percent_Identity=24.5487364620939, Blast_Score=102, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.-.- [C]

Molecular weight: Translated: 32016; Mature: 32016

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAVTNATEFRLGRVAVIGLGSMGYGMAQSLKRAGFDVTGCDTNQDAVARFVAEGGRSSP
CCCCCCCHHHCCCEEEEEEECCCCHHHHHHHHHCCCEEECCCCCHHHHHHHHHCCCCCCC
ILADVAADADCVISVVVNAAQTEAILFGDGGVAETMPKDSVFISCATMDPDIVRRLAAKL
EEEEHHCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHHH
EATGRHYLDAPISGGAKRAAEGALTILASGSPKAFAKARPALNAMAAKLYELGDAPGQGA
HHCCCEEECCCCCCCHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCH
AFKMINQLLAGVHIAVASEAITFAARQGLDLDKVYEVITASAGNSWMFENRVPHVLEGDY
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEECCCCCHHCCCCC
TPRSAVDIFVKDLGIIQDMARNTKFPVPIAAAALQMFLMTSAAGMGREDDASVARLYANI
CCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHC
TGTKLPGA
CCCCCCCC
>Mature Secondary Structure
MNAVTNATEFRLGRVAVIGLGSMGYGMAQSLKRAGFDVTGCDTNQDAVARFVAEGGRSSP
CCCCCCCHHHCCCEEEEEEECCCCHHHHHHHHHCCCEEECCCCCHHHHHHHHHCCCCCCC
ILADVAADADCVISVVVNAAQTEAILFGDGGVAETMPKDSVFISCATMDPDIVRRLAAKL
EEEEHHCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHHH
EATGRHYLDAPISGGAKRAAEGALTILASGSPKAFAKARPALNAMAAKLYELGDAPGQGA
HHCCCEEECCCCCCCHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCH
AFKMINQLLAGVHIAVASEAITFAARQGLDLDKVYEVITASAGNSWMFENRVPHVLEGDY
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEECCCCCHHCCCCC
TPRSAVDIFVKDLGIIQDMARNTKFPVPIAAAALQMFLMTSAAGMGREDDASVARLYANI
CCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHC
TGTKLPGA
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]