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The map label for this gene is hmp [H]

Identifier: 182678938

GI number: 182678938

Start: 2252320

End: 2253531

Strand: Reverse

Name: hmp [H]

Synonym: Bind_1972

Alternate gene names: 182678938

Gene position: 2253531-2252320 (Counterclockwise)

Preceding gene: 182678941

Following gene: 182678937

Centisome position: 54.04

GC content: 57.59

Gene sequence:

>1212_bases
ATGGCCGAGCCTCTTTCTCCAGAAACCATTGCCCTCATCAAGGCGACAGTGCCGGCGCTGGAAGCGCATGGCCTGGACAT
TACGAGGGAAATGTATGCGCGCTTGCTGCAGAACCCGGCCATTAAATCCCTCTTCAACCAGTCCCATCAAGGCGATGGTG
CGCAGCCTCGGGCTCTTGCCATGGCGGTTTTGGCCTATGCCAAGAATATCGATAATCTTGGCATGCTGGCTGGCGCCGCC
GTCGAACGCATTGCTCAGAAACATGTCGGCCTGAACATTCTGGCGGAACATTATCCTTATGTGGGAGAGGCTTTGATCGG
CGCGATCCGGGCGGTTCTTGGCGACGCCGCCACACCGGAGATTATCGACGCCTGGGGCAAGGCCTATTGGTTTCTCGCTG
ATCTGCTCATCGGCCGCGAGAAGCAGATTTATGACGCGCATGAACAAGCGCCGGGCGGCTGGACCGGCTGGCGGGACTTT
ACCATCGCGCGCCGTATAGACGAAAGCGACACCATCACATCCTTCGAACTGGCTCCCTCGGATGGCAAGGAGGTTCTCCG
GCATCGGCCGGGCCAATATCTGAGCTTTGCTTTCGATCTTGCGGACAAGGGATCCTTCCGCCGTAATTACAGCATTTCTT
CCGCGCCTAGCCAGGAGAGCTATCGGATCAGTGTCAAACGTGCCGACCAGGGAAAAATATCGAACTGGCTGCATGATGAG
GGGAAGATCGGCGTCACGCTGAAGGTCTCGGCACCAGCCGGAGAATTCGCGCTGCCAGCTTCGGAAACATGCCCGCTCGT
TCTGTTGAGCGGGGGCGTCGGGCTGACACCGATGATCAGCATGCTGGAGGATCTTGCGCAAACGGATTCGCAAGTGCCTG
TGCGGTTCATCCATGCCACACATTCAGGCGCGACCCATGCGTTTGGAGCGCGTGTGCGTGCCCTGTCGGACGCTTCGAAA
GGGCGTATTCGTCCGACGATTTTCTACAGTGCGCCGCGTCTCGAGGATGAAATTGGCGTCGCTTATGACAAAGCCGGTCA
TGTGACCGCCGCCTGGTTGCGCGCCGAAATGCCGGAAGATGCGGATTATTATGTCTGTGGCCCGCATGAATTTCAGCGTG
ATCTCATCAGTGGCCTGCGTGCATCGGGAGTTGCCGATGCGCGGATTCATTATGAATTTTTCGGTCCCACCGATGAATTT
CTGACCAGCTGA

Upstream 100 bases:

>100_bases
TCGGTGTGCAGGGTCAGGCGCATGGATAAGATGTACCATCTTTACATCTTATTTCCAGAGGTTATAAGAGGTATATTGTT
TACCCCTTATAGGAGCGATC

Downstream 100 bases:

>100_bases
GATGGGAAGGGTATAAAAGGCGCATTCCGAACAAAGACTGTTGGCATTCTTACAGGCAGTCTGCATAATAATACATCAGA
ACCCCCAAGGCGCTTGCCGT

Product: oxidoreductase FAD-binding subunit

Products: NA

Alternate protein names: Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD [H]

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MAEPLSPETIALIKATVPALEAHGLDITREMYARLLQNPAIKSLFNQSHQGDGAQPRALAMAVLAYAKNIDNLGMLAGAA
VERIAQKHVGLNILAEHYPYVGEALIGAIRAVLGDAATPEIIDAWGKAYWFLADLLIGREKQIYDAHEQAPGGWTGWRDF
TIARRIDESDTITSFELAPSDGKEVLRHRPGQYLSFAFDLADKGSFRRNYSISSAPSQESYRISVKRADQGKISNWLHDE
GKIGVTLKVSAPAGEFALPASETCPLVLLSGGVGLTPMISMLEDLAQTDSQVPVRFIHATHSGATHAFGARVRALSDASK
GRIRPTIFYSAPRLEDEIGVAYDKAGHVTAAWLRAEMPEDADYYVCGPHEFQRDLISGLRASGVADARIHYEFFGPTDEF
LTS

Sequences:

>Translated_403_residues
MAEPLSPETIALIKATVPALEAHGLDITREMYARLLQNPAIKSLFNQSHQGDGAQPRALAMAVLAYAKNIDNLGMLAGAA
VERIAQKHVGLNILAEHYPYVGEALIGAIRAVLGDAATPEIIDAWGKAYWFLADLLIGREKQIYDAHEQAPGGWTGWRDF
TIARRIDESDTITSFELAPSDGKEVLRHRPGQYLSFAFDLADKGSFRRNYSISSAPSQESYRISVKRADQGKISNWLHDE
GKIGVTLKVSAPAGEFALPASETCPLVLLSGGVGLTPMISMLEDLAQTDSQVPVRFIHATHSGATHAFGARVRALSDASK
GRIRPTIFYSAPRLEDEIGVAYDKAGHVTAAWLRAEMPEDADYYVCGPHEFQRDLISGLRASGVADARIHYEFFGPTDEF
LTS
>Mature_402_residues
AEPLSPETIALIKATVPALEAHGLDITREMYARLLQNPAIKSLFNQSHQGDGAQPRALAMAVLAYAKNIDNLGMLAGAAV
ERIAQKHVGLNILAEHYPYVGEALIGAIRAVLGDAATPEIIDAWGKAYWFLADLLIGREKQIYDAHEQAPGGWTGWRDFT
IARRIDESDTITSFELAPSDGKEVLRHRPGQYLSFAFDLADKGSFRRNYSISSAPSQESYRISVKRADQGKISNWLHDEG
KIGVTLKVSAPAGEFALPASETCPLVLLSGGVGLTPMISMLEDLAQTDSQVPVRFIHATHSGATHAFGARVRALSDASKG
RIRPTIFYSAPRLEDEIGVAYDKAGHVTAAWLRAEMPEDADYYVCGPHEFQRDLISGLRASGVADARIHYEFFGPTDEFL
TS

Specific function: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a centra

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788903, Length=399, Percent_Identity=40.3508771929825, Blast_Score=273, Evalue=1e-74,
Organism=Escherichia coli, GI1787658, Length=248, Percent_Identity=25.4032258064516, Blast_Score=82, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6321673, Length=410, Percent_Identity=38.2926829268293, Blast_Score=239, Evalue=6e-64,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017927
- InterPro:   IPR012292
- InterPro:   IPR009050
- InterPro:   IPR000971
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR000951
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00042 Globin; PF00175 NAD_binding_1 [H]

EC number: =1.14.12.17 [H]

Molecular weight: Translated: 43932; Mature: 43801

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00196 COPPER_BLUE ; PS01033 GLOBIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEPLSPETIALIKATVPALEAHGLDITREMYARLLQNPAIKSLFNQSHQGDGAQPRALA
CCCCCCCCCEEEEHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHH
MAVLAYAKNIDNLGMLAGAAVERIAQKHVGLNILAEHYPYVGEALIGAIRAVLGDAATPE
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCHH
IIDAWGKAYWFLADLLIGREKQIYDAHEQAPGGWTGWRDFTIARRIDESDTITSFELAPS
HHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCEEEEEECCC
DGKEVLRHRPGQYLSFAFDLADKGSFRRNYSISSAPSQESYRISVKRADQGKISNWLHDE
CHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHCCCC
GKIGVTLKVSAPAGEFALPASETCPLVLLSGGVGLTPMISMLEDLAQTDSQVPVRFIHAT
CCEEEEEEEECCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEEC
HSGATHAFGARVRALSDASKGRIRPTIFYSAPRLEDEIGVAYDKAGHVTAAWLRAEMPED
CCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHCCEECCCCCCEEEHHHHCCCCC
ADYYVCGPHEFQRDLISGLRASGVADARIHYEFFGPTDEFLTS
CCEEEECCHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHCC
>Mature Secondary Structure 
AEPLSPETIALIKATVPALEAHGLDITREMYARLLQNPAIKSLFNQSHQGDGAQPRALA
CCCCCCCCEEEEHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHH
MAVLAYAKNIDNLGMLAGAAVERIAQKHVGLNILAEHYPYVGEALIGAIRAVLGDAATPE
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCHH
IIDAWGKAYWFLADLLIGREKQIYDAHEQAPGGWTGWRDFTIARRIDESDTITSFELAPS
HHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCEEEEEECCC
DGKEVLRHRPGQYLSFAFDLADKGSFRRNYSISSAPSQESYRISVKRADQGKISNWLHDE
CHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHCCCC
GKIGVTLKVSAPAGEFALPASETCPLVLLSGGVGLTPMISMLEDLAQTDSQVPVRFIHAT
CCEEEEEEEECCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEEC
HSGATHAFGARVRALSDASKGRIRPTIFYSAPRLEDEIGVAYDKAGHVTAAWLRAEMPED
CCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHCCEECCCCCCEEEHHHHCCCCC
ADYYVCGPHEFQRDLISGLRASGVADARIHYEFFGPTDEFLTS
CCEEEECCHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]