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The map label for this gene is nasA [H]

Identifier: 182678937

GI number: 182678937

Start: 2250684

End: 2251955

Strand: Reverse

Name: nasA [H]

Synonym: Bind_1971

Alternate gene names: 182678937

Gene position: 2251955-2250684 (Counterclockwise)

Preceding gene: 182678938

Following gene: 182678936

Centisome position: 54.0

GC content: 59.28

Gene sequence:

>1272_bases
ATGGATAGAGGTTTTCTGAAAGCAGGGCATCTGCCGACACTCTTTGCCGCCTTTCTCTATTTTGACATTTCCTTCATGGT
TTGGGTGCTGCTGGGTCCGCTCGGGATCTCGATAGCGCAGGACCTCCATCTCAATCCCGCTCAAAAAGGCTTCATGGTCG
CGACACCCGTTCTGGCGGGCGCCTTTTTGCGTGTTGTGCTGGGGGCGTTGGTCGATCGCTTCGGCGCCCGCGCTGTCGGT
ATTGGAGCCCAGATCGTTGTCATTCTGGGGCTCGTTACGACGTGGCTGATCGCGCCGCATTCCTATGAATATATGTTTGT
GCTGGCTCTGGTGCTCGGCCTCGCGGGTGCGTCCTTCGCGGTCGCCTTGCCTTTGGCTTCGTCCTGGTATCCGCCCAAAT
ATCAGGGCACGGCTTTGGGGATTGCCGGTGCGGGTAATTCCGGCACGGCGGTGGCCGCCTTGTTCGCGCCGGGCCTTGCC
GTCCTTTATGGCTGGCTGAATGTGATCGGTCTGGCGGCGATACCGCTTGTCATCGTGCTGGTCATCTTTATCGCGCTCGC
AAAAGACTCTCCGAAACGTCCCCCAGCGCGTCCGCTTTCGGCCTGGTTGCCTCTTCTGCGGCAGGCCGATTGTTGGTGGT
TCATGTTTTTCTACAGCGTCACTTTCGGCGGCTTCGTCGGGCTTGCAGCTTCGTTGACGATCTATTTCAACGATCAATAT
GGGTTGCCGCCGGCCATGGCTGGCCTGTGTACGGCGCTGATTGTTTTCGCCGGTTCGTTTGTCCGGCCGGTCGGTGGCGT
GATTGCTGATCGCGTGGGCGGTGTCCGGGCTCTGGTTGTCTTCTATGCCGTGGCGGCCATTGTTTTCGCCATTGTGGCTG
CCGGGCTCCCTTCGATCTGGCTGGCCATGCCGGTTTTTATTCTCGGCATGCTGGCTTTGGGTATGGGCAATGGGGCGGTC
TTCCAGCTCGTGCCGCAACATTTCAGTCATGCCATTGGAATTATGACTGGATTGGTGGGGATGAGTGGCGGCGTCGGCGG
CTTCTATTTAGCTTCGTCGCTGGGCGCCGCGCGGCAGCTCACCGGTTCCTATGGCGCGGGCTTTCTGTTGTTCAGCCTGC
TGGCCGTGGCTGCTCTCTTGGGCATCCTCTATTGCCAGCGCCGTTGGCGCGTCACCTCCTCGGTTGAAGAACAAGTTGGA
GAGATCGGGGAAGTCGCGGGGACGCGTAAGGCATCTGTGTATCTCGCTGTCCCGTTTGCAACTCTAGCCTGA

Upstream 100 bases:

>100_bases
TGAAAAGCCTTTGTGACGAACTGGTGGGTCAACGGCGGCCTGCTTGGCCCCAAGGGGCGACGATGGAACTGGAGTACTTC
CTCGTCATCGGATCAGACCT

Downstream 100 bases:

>100_bases
TTTTCTGAAAAGCCACGTCTCCCATCAAGAGACTGTCACGTCTTGTGTGTGCGTGCCCGATCCTGTTCGGGTTCGCCCCA
CCGTATGGGGCCATTGCGCG

Product: major facilitator transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MDRGFLKAGHLPTLFAAFLYFDISFMVWVLLGPLGISIAQDLHLNPAQKGFMVATPVLAGAFLRVVLGALVDRFGARAVG
IGAQIVVILGLVTTWLIAPHSYEYMFVLALVLGLAGASFAVALPLASSWYPPKYQGTALGIAGAGNSGTAVAALFAPGLA
VLYGWLNVIGLAAIPLVIVLVIFIALAKDSPKRPPARPLSAWLPLLRQADCWWFMFFYSVTFGGFVGLAASLTIYFNDQY
GLPPAMAGLCTALIVFAGSFVRPVGGVIADRVGGVRALVVFYAVAAIVFAIVAAGLPSIWLAMPVFILGMLALGMGNGAV
FQLVPQHFSHAIGIMTGLVGMSGGVGGFYLASSLGAARQLTGSYGAGFLLFSLLAVAALLGILYCQRRWRVTSSVEEQVG
EIGEVAGTRKASVYLAVPFATLA

Sequences:

>Translated_423_residues
MDRGFLKAGHLPTLFAAFLYFDISFMVWVLLGPLGISIAQDLHLNPAQKGFMVATPVLAGAFLRVVLGALVDRFGARAVG
IGAQIVVILGLVTTWLIAPHSYEYMFVLALVLGLAGASFAVALPLASSWYPPKYQGTALGIAGAGNSGTAVAALFAPGLA
VLYGWLNVIGLAAIPLVIVLVIFIALAKDSPKRPPARPLSAWLPLLRQADCWWFMFFYSVTFGGFVGLAASLTIYFNDQY
GLPPAMAGLCTALIVFAGSFVRPVGGVIADRVGGVRALVVFYAVAAIVFAIVAAGLPSIWLAMPVFILGMLALGMGNGAV
FQLVPQHFSHAIGIMTGLVGMSGGVGGFYLASSLGAARQLTGSYGAGFLLFSLLAVAALLGILYCQRRWRVTSSVEEQVG
EIGEVAGTRKASVYLAVPFATLA
>Mature_423_residues
MDRGFLKAGHLPTLFAAFLYFDISFMVWVLLGPLGISIAQDLHLNPAQKGFMVATPVLAGAFLRVVLGALVDRFGARAVG
IGAQIVVILGLVTTWLIAPHSYEYMFVLALVLGLAGASFAVALPLASSWYPPKYQGTALGIAGAGNSGTAVAALFAPGLA
VLYGWLNVIGLAAIPLVIVLVIFIALAKDSPKRPPARPLSAWLPLLRQADCWWFMFFYSVTFGGFVGLAASLTIYFNDQY
GLPPAMAGLCTALIVFAGSFVRPVGGVIADRVGGVRALVVFYAVAAIVFAIVAAGLPSIWLAMPVFILGMLALGMGNGAV
FQLVPQHFSHAIGIMTGLVGMSGGVGGFYLASSLGAARQLTGSYGAGFLLFSLLAVAALLGILYCQRRWRVTSSVEEQVG
EIGEVAGTRKASVYLAVPFATLA

Specific function: May function as a nitrate transporter [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=410, Percent_Identity=24.8780487804878, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1787743, Length=422, Percent_Identity=26.7772511848341, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 44236; Mature: 44236

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HCC
>Mature Secondary Structure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HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]