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The map label for this gene is rlmE [H]

Identifier: 182678893

GI number: 182678893

Start: 2196006

End: 2196710

Strand: Reverse

Name: rlmE [H]

Synonym: Bind_1926

Alternate gene names: 182678893

Gene position: 2196710-2196006 (Counterclockwise)

Preceding gene: 182678894

Following gene: 182678890

Centisome position: 52.68

GC content: 60.43

Gene sequence:

>705_bases
ATGAACGATGTTTCTTCTTCTGGCCGCGAGGGCGGCCGCTCGCTGAAGACGCGGGTCAAGACTGCACGCAAGCGGTCCTT
GTCCTCGACGCTTTGGCTTGAGCGCCAGCTCAACGATCCTTATGTCGCTCGCGCCAAGCGTGAGGGCATGCGCTCGCGCG
CCGCCTATAAACTCATCGAAATCGACGAGCGCTATCCCTTTCTGAAACCGGGGCAGAAAGTCGTCGATCTTGGCGCTGCA
CCCGGTGGCTGGTCGCAAGTCGCTGTGCGCAAAGTCCAGAGCGAGCAGGGGAAAGGGCGCGTCATCGGCATAGACCTTCT
GGAAGTTGATCCCATGCCGGGCGCCGAATTCAAGGTCCTGGATTTTCTCGATCCGGCCGCGCCCGAAACGCTCGAACATT
GGCTCGGCGCGAAGGCGGATGTCGTCTTGTCGGATATGGCGGCCAATGCGACAGGCCATAAGAAGACCGATCATTTGCGA
ATCGTCGGCCTGGTGGAATTGGCGGCGGATTTCGCCAGCACGATCCTGGCGCCGGGCGGCAGCTTTCTCGCCAAGGTCTT
GCAGGGCGGCACAGAAAATAGCCTGCTCGCAAAGCTGAAACAGGAATTTGCCAGCGTGCGGCATGTGAAGCCGCAAGCGA
GCCGCGCCGATTCCTCGGAGCTTTATATGCTCGCCACGGGATTTCGAGGTAAGGCCTCGGCTTGA

Upstream 100 bases:

>100_bases
GTGCGCATTGCCGACCGTGGTCTGCGCGAAGGCATTTTGATGCAAATGATTAACGCGGACCGGTTGCACGCCGGGACGGA
AGGGCGCTAAAGCCTCCTCC

Downstream 100 bases:

>100_bases
GCGTCGCAAATAGTCGCTCACTTCATCGATGATGTCGTCACTCTTTGGCTCGCACCACGAGAATCGGGCAGGGAGACTTG
CGCATCACGGCCTCGGCGAC

Product: ribosomal RNA methyltransferase RrmJ/FtsJ

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase [H]

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MNDVSSSGREGGRSLKTRVKTARKRSLSSTLWLERQLNDPYVARAKREGMRSRAAYKLIEIDERYPFLKPGQKVVDLGAA
PGGWSQVAVRKVQSEQGKGRVIGIDLLEVDPMPGAEFKVLDFLDPAAPETLEHWLGAKADVVLSDMAANATGHKKTDHLR
IVGLVELAADFASTILAPGGSFLAKVLQGGTENSLLAKLKQEFASVRHVKPQASRADSSELYMLATGFRGKASA

Sequences:

>Translated_234_residues
MNDVSSSGREGGRSLKTRVKTARKRSLSSTLWLERQLNDPYVARAKREGMRSRAAYKLIEIDERYPFLKPGQKVVDLGAA
PGGWSQVAVRKVQSEQGKGRVIGIDLLEVDPMPGAEFKVLDFLDPAAPETLEHWLGAKADVVLSDMAANATGHKKTDHLR
IVGLVELAADFASTILAPGGSFLAKVLQGGTENSLLAKLKQEFASVRHVKPQASRADSSELYMLATGFRGKASA
>Mature_234_residues
MNDVSSSGREGGRSLKTRVKTARKRSLSSTLWLERQLNDPYVARAKREGMRSRAAYKLIEIDERYPFLKPGQKVVDLGAA
PGGWSQVAVRKVQSEQGKGRVIGIDLLEVDPMPGAEFKVLDFLDPAAPETLEHWLGAKADVVLSDMAANATGHKKTDHLR
IVGLVELAADFASTILAPGGSFLAKVLQGGTENSLLAKLKQEFASVRHVKPQASRADSSELYMLATGFRGKASA

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit [H]

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family [H]

Homologues:

Organism=Homo sapiens, GI7019377, Length=233, Percent_Identity=44.206008583691, Blast_Score=188, Evalue=3e-48,
Organism=Homo sapiens, GI29029591, Length=191, Percent_Identity=35.6020942408377, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI29029589, Length=191, Percent_Identity=35.6020942408377, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI7110661, Length=191, Percent_Identity=35.6020942408377, Blast_Score=119, Evalue=2e-27,
Organism=Homo sapiens, GI194097365, Length=191, Percent_Identity=35.6020942408377, Blast_Score=112, Evalue=3e-25,
Organism=Escherichia coli, GI1789569, Length=208, Percent_Identity=46.1538461538462, Blast_Score=176, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17553474, Length=204, Percent_Identity=38.2352941176471, Blast_Score=144, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17554650, Length=199, Percent_Identity=33.1658291457286, Blast_Score=120, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI71987550, Length=199, Percent_Identity=32.6633165829146, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71987561, Length=199, Percent_Identity=32.6633165829146, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17553860, Length=199, Percent_Identity=32.6633165829146, Blast_Score=95, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6319535, Length=199, Percent_Identity=32.6633165829146, Blast_Score=105, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6319796, Length=194, Percent_Identity=32.4742268041237, Blast_Score=89, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6321302, Length=98, Percent_Identity=41.8367346938776, Blast_Score=84, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21356387, Length=227, Percent_Identity=40.0881057268723, Blast_Score=154, Evalue=4e-38,
Organism=Drosophila melanogaster, GI18859957, Length=191, Percent_Identity=37.696335078534, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24647580, Length=198, Percent_Identity=32.8282828282828, Blast_Score=101, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24648639, Length=196, Percent_Identity=33.6734693877551, Blast_Score=101, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015507
- InterPro:   IPR002877 [H]

Pfam domain/function: PF01728 FtsJ [H]

EC number: =2.1.1.166 [H]

Molecular weight: Translated: 25341; Mature: 25341

Theoretical pI: Translated: 10.31; Mature: 10.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDVSSSGREGGRSLKTRVKTARKRSLSSTLWLERQLNDPYVARAKREGMRSRAAYKLIE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEE
IDERYPFLKPGQKVVDLGAAPGGWSQVAVRKVQSEQGKGRVIGIDLLEVDPMPGAEFKVL
ECCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCEEEE
DFLDPAAPETLEHWLGAKADVVLSDMAANATGHKKTDHLRIVGLVELAADFASTILAPGG
ECCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCH
SFLAKVLQGGTENSLLAKLKQEFASVRHVKPQASRADSSELYMLATGFRGKASA
HHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCC
>Mature Secondary Structure
MNDVSSSGREGGRSLKTRVKTARKRSLSSTLWLERQLNDPYVARAKREGMRSRAAYKLIE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEE
IDERYPFLKPGQKVVDLGAAPGGWSQVAVRKVQSEQGKGRVIGIDLLEVDPMPGAEFKVL
ECCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCEEEE
DFLDPAAPETLEHWLGAKADVVLSDMAANATGHKKTDHLRIVGLVELAADFASTILAPGG
ECCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCH
SFLAKVLQGGTENSLLAKLKQEFASVRHVKPQASRADSSELYMLATGFRGKASA
HHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA