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The map label for this gene is gatB

Identifier: 182678844

GI number: 182678844

Start: 2140541

End: 2142028

Strand: Reverse

Name: gatB

Synonym: Bind_1877

Alternate gene names: 182678844

Gene position: 2142028-2140541 (Counterclockwise)

Preceding gene: 182678845

Following gene: 182678840

Centisome position: 51.37

GC content: 59.07

Gene sequence:

>1488_bases
ATGAACACACATGTCAAACCTTCCAAGCTCATCAAGGGCGCGACCGGCGATTGGGAAATCATCATTGGTCTTGAAGTCCA
TGCCCAGGTGACATCGCGATCCAAACTCTTTTCCGGTGCCTCCACGCAATTCGGAGCCGAGCCGAACAGCAATGTTTCGC
TCGTCGATGCCGCCATGCCGGGCATGCTGCCGGTCATCAACGAGGAATGCGTGGCGCAGGCCGTGCGCACGGGTCTCGGC
CTCAAGGCTCAGATCAATCTGCGTTCTGTCTTCGATCGGAAGAACTATTTCTATCCCGATCTGCCGCAGGGCTATCAGAT
CTCCCAGTACAAGAGCCCTATCGTCGGCGAGGGCATCGTGATTGTCGATGTGTCGCCGACCGAACAGATCGAGGTCGGGA
TCGAGCGTCTGCATCTCGAACAGGATGCCGGCAAATCACTGCACGACCAATCAGCGACGGATTCCTTCGTCGATCTCAAC
CGGTCGGGTGTGGCCTTGATGGAGATCGTGTCCAAACCCGACATGCGATCCGCCGACGAGGCGCGCGCTTATGTCAGCAA
GCTGCGCACCATCCTGCGCTATCTTGGCACGTGCGACGGCAATATGGAGCAGGGCTCACTGCGCGCCGACGTCAATGTGT
CGGTCCGCCGGCCGGGCGAGCCGTTCGGCACGCGCTGCGAGATCAAGAACGTCAATTCGATCCGTTTCATCGGCCAGGCG
ATCGAGACGGAAGCCCGCCGGCAGATCGGTATCCTCGAGGATGGCGGCGTGATCGATCAGGAGACGCGCTTGTTCGATCC
GGGCAAGGGCGAGACGCGCTCGATGCGTTCGAAGGAAGAAGCGCATGACTATCGCTACTTCCCTGATCCCGATCTGTTGC
CGCTCGAATTCGATCAGGCCTATGTCGATGGGCTGGCCTCCGCGCTGCCGGAATTGCCGGATGCGAAGAAGGCTCGCTTC
ATGAACGATTACGGGCTGCCGGCCTATGACGCGGGCGTGCTCGTCGCCGACAAGGAGACGGCCGATTATTATGAGACGAC
CGTCAATTATAACGGCGTGAAACGCGATCCCAAGCTCGTCGCCAATTGGGTGACGGGCGATGTGGCGGCCTATGCCAATT
CGGTCGGGCTGCCGGTTTCGCAAACCCATCTTACACCAGCGCAGATCGCCACATTGGTCGATCTCATCAGCGAAGAAGTG
ATCTCGGGCAAGATCGCGAAGGATGTGCTGGCGATCCTGATCAATGAGGACAAGGCGGGTGATCCACGCACTATCGTCGA
GGCACGTGGCCTGAAACAGGTCACGGATACGGGTGCTATTGAAGCAGCCGTGGATGCGATCATCGCCGCCAATCCAGACA
AGGTGGCGCAGGCGCAGGCCAAGCCCACAATGCTCGGCTGGTTCGTGGGGCAGGTGATGAAACAGACCGGCGGCAAAGCC
AACCCGCAAGCCGTCAATGAAATACTGAAAGCCAAACTCGGGATCTAA

Upstream 100 bases:

>100_bases
AGGAGCACGGCGACGCAAAGCTATTGGGTTATGCGTCGCTGCCGCGAATGAGGTATAAGATTTCAAGAGACGAGCGTGGG
CTCGAACAAAGGGGTCCTGC

Downstream 100 bases:

>100_bases
CGATCGAACGAGAGAACGATCGGAGGCGGCTGAAAAGCATTCGGCTTTTGCATCCGAATGGCGAATCAATCAACGATGAT
TCGTCGAAGCCGTTCACGAA

Product: glutamyl-tRNA(Gln) amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MNTHVKPSKLIKGATGDWEIIIGLEVHAQVTSRSKLFSGASTQFGAEPNSNVSLVDAAMPGMLPVINEECVAQAVRTGLG
LKAQINLRSVFDRKNYFYPDLPQGYQISQYKSPIVGEGIVIVDVSPTEQIEVGIERLHLEQDAGKSLHDQSATDSFVDLN
RSGVALMEIVSKPDMRSADEARAYVSKLRTILRYLGTCDGNMEQGSLRADVNVSVRRPGEPFGTRCEIKNVNSIRFIGQA
IETEARRQIGILEDGGVIDQETRLFDPGKGETRSMRSKEEAHDYRYFPDPDLLPLEFDQAYVDGLASALPELPDAKKARF
MNDYGLPAYDAGVLVADKETADYYETTVNYNGVKRDPKLVANWVTGDVAAYANSVGLPVSQTHLTPAQIATLVDLISEEV
ISGKIAKDVLAILINEDKAGDPRTIVEARGLKQVTDTGAIEAAVDAIIAANPDKVAQAQAKPTMLGWFVGQVMKQTGGKA
NPQAVNEILKAKLGI

Sequences:

>Translated_495_residues
MNTHVKPSKLIKGATGDWEIIIGLEVHAQVTSRSKLFSGASTQFGAEPNSNVSLVDAAMPGMLPVINEECVAQAVRTGLG
LKAQINLRSVFDRKNYFYPDLPQGYQISQYKSPIVGEGIVIVDVSPTEQIEVGIERLHLEQDAGKSLHDQSATDSFVDLN
RSGVALMEIVSKPDMRSADEARAYVSKLRTILRYLGTCDGNMEQGSLRADVNVSVRRPGEPFGTRCEIKNVNSIRFIGQA
IETEARRQIGILEDGGVIDQETRLFDPGKGETRSMRSKEEAHDYRYFPDPDLLPLEFDQAYVDGLASALPELPDAKKARF
MNDYGLPAYDAGVLVADKETADYYETTVNYNGVKRDPKLVANWVTGDVAAYANSVGLPVSQTHLTPAQIATLVDLISEEV
ISGKIAKDVLAILINEDKAGDPRTIVEARGLKQVTDTGAIEAAVDAIIAANPDKVAQAQAKPTMLGWFVGQVMKQTGGKA
NPQAVNEILKAKLGI
>Mature_495_residues
MNTHVKPSKLIKGATGDWEIIIGLEVHAQVTSRSKLFSGASTQFGAEPNSNVSLVDAAMPGMLPVINEECVAQAVRTGLG
LKAQINLRSVFDRKNYFYPDLPQGYQISQYKSPIVGEGIVIVDVSPTEQIEVGIERLHLEQDAGKSLHDQSATDSFVDLN
RSGVALMEIVSKPDMRSADEARAYVSKLRTILRYLGTCDGNMEQGSLRADVNVSVRRPGEPFGTRCEIKNVNSIRFIGQA
IETEARRQIGILEDGGVIDQETRLFDPGKGETRSMRSKEEAHDYRYFPDPDLLPLEFDQAYVDGLASALPELPDAKKARF
MNDYGLPAYDAGVLVADKETADYYETTVNYNGVKRDPKLVANWVTGDVAAYANSVGLPVSQTHLTPAQIATLVDLISEEV
ISGKIAKDVLAILINEDKAGDPRTIVEARGLKQVTDTGAIEAAVDAIIAANPDKVAQAQAKPTMLGWFVGQVMKQTGGKA
NPQAVNEILKAKLGI

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=498, Percent_Identity=40.1606425702811, Blast_Score=364, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI115534094, Length=388, Percent_Identity=36.0824742268041, Blast_Score=222, Evalue=3e-58,
Organism=Saccharomyces cerevisiae, GI6319391, Length=522, Percent_Identity=30.6513409961686, Blast_Score=230, Evalue=4e-61,
Organism=Drosophila melanogaster, GI45550808, Length=491, Percent_Identity=37.2708757637475, Blast_Score=325, Evalue=3e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_BEII9 (B2IEA3)

Other databases:

- EMBL:   CP001016
- RefSeq:   YP_001832990.1
- GeneID:   6201083
- GenomeReviews:   CP001016_GR
- KEGG:   bid:Bind_1877
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- ProtClustDB:   CLSK2405596
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- Gene3D:   G3DSA:1.10.10.410
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 53758; Mature: 53758

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTHVKPSKLIKGATGDWEIIIGLEVHAQVTSRSKLFSGASTQFGAEPNSNVSLVDAAMP
CCCCCCHHHHHCCCCCCEEEEEEEEEEEEHHHHHHHHCCCCCCCCCCCCCCEEEEECCCC
GMLPVINEECVAQAVRTGLGLKAQINLRSVFDRKNYFYPDLPQGYQISQYKSPIVGEGIV
CCCHHHCHHHHHHHHHHCCCCEEEEHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCCCCEE
IVDVSPTEQIEVGIERLHLEQDAGKSLHDQSATDSFVDLNRSGVALMEIVSKPDMRSADE
EEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCHHHHHHHCCCCCCCHHH
ARAYVSKLRTILRYLGTCDGNMEQGSLRADVNVSVRRPGEPFGTRCEIKNVNSIRFIGQA
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCCCEEEECCCCHHHHHHHH
IETEARRQIGILEDGGVIDQETRLFDPGKGETRSMRSKEEAHDYRYFPDPDLLPLEFDQA
HHHHHHHHCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
YVDGLASALPELPDAKKARFMNDYGLPAYDAGVLVADKETADYYETTVNYNGVKRDPKLV
HHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCEEEECCCCCCHHHHCCCCCCCCCCHHHH
ANWVTGDVAAYANSVGLPVSQTHLTPAQIATLVDLISEEVISGKIAKDVLAILINEDKAG
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHEECCCCCC
DPRTIVEARGLKQVTDTGAIEAAVDAIIAANPDKVAQAQAKPTMLGWFVGQVMKQTGGKA
CCHHHHHHCCCHHHHCCCHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCC
NPQAVNEILKAKLGI
CHHHHHHHHHHHCCC
>Mature Secondary Structure
MNTHVKPSKLIKGATGDWEIIIGLEVHAQVTSRSKLFSGASTQFGAEPNSNVSLVDAAMP
CCCCCCHHHHHCCCCCCEEEEEEEEEEEEHHHHHHHHCCCCCCCCCCCCCCEEEEECCCC
GMLPVINEECVAQAVRTGLGLKAQINLRSVFDRKNYFYPDLPQGYQISQYKSPIVGEGIV
CCCHHHCHHHHHHHHHHCCCCEEEEHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCCCCEE
IVDVSPTEQIEVGIERLHLEQDAGKSLHDQSATDSFVDLNRSGVALMEIVSKPDMRSADE
EEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCHHHHHHHCCCCCCCHHH
ARAYVSKLRTILRYLGTCDGNMEQGSLRADVNVSVRRPGEPFGTRCEIKNVNSIRFIGQA
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCCCEEEECCCCHHHHHHHH
IETEARRQIGILEDGGVIDQETRLFDPGKGETRSMRSKEEAHDYRYFPDPDLLPLEFDQA
HHHHHHHHCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
YVDGLASALPELPDAKKARFMNDYGLPAYDAGVLVADKETADYYETTVNYNGVKRDPKLV
HHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCEEEECCCCCCHHHHCCCCCCCCCCHHHH
ANWVTGDVAAYANSVGLPVSQTHLTPAQIATLVDLISEEVISGKIAKDVLAILINEDKAG
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHEECCCCCC
DPRTIVEARGLKQVTDTGAIEAAVDAIIAANPDKVAQAQAKPTMLGWFVGQVMKQTGGKA
CCHHHHHHCCCHHHHCCCHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCC
NPQAVNEILKAKLGI
CHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA