Click here to switch to the map view.

The map label for this gene is aes [H]

Identifier: 182678832

GI number: 182678832

Start: 2125553

End: 2126569

Strand: Direct

Name: aes [H]

Synonym: Bind_1865

Alternate gene names: 182678832

Gene position: 2125553-2126569 (Clockwise)

Preceding gene: 182678831

Following gene: 182678833

Centisome position: 50.97

GC content: 55.56

Gene sequence:

>1017_bases
ATGAAAAAAATCATGCTGGTATCGGTCACCGCGCTGAGCCTTGTGGCGAGCAGTCATAAGGCACCCGCCGAGCCGCAGGA
CACGAAAAAAGAGGTGTTCGCGATCCGCGAGGGTATGAACAATGGCAATGCTGGCATGGAACAAGGCCTCGCCAAACATA
TTCCCGACGGTGTCGCCTCCGTGCTCGACGAGGCATACACCCAAGGCGACGCCGATGCCCGCCTCGATGTCTTTTACCCC
GCCTCACTCGCTCAGTCAGGCCAGGCTTTGCAGACGATCGTCTGGCTGCATGGGGGCGGTTGGGTCTCTGGCGACAAGGA
TTATGTCGCTCCTTATCTCCAGATTCTCGCCGCGCGTGGCTTCACCGTTGTCGGCCTCAATTATTCACTCGCGCCAGAGC
ATCATTATCCGGCACCGCTCCAACAGATCGATGCGGCCCTTGCTTATCTTCAAAAAAATGCGGCGCGCCTGCATATCGAT
CCCAACAGATTTTTCCTCGCGGGCGATTCAGCAGGTGCCCAGCTTGCCGGCCAGTTCGGCAATATCGTCACCGATCCGGT
TTATGCGCGAGACCTCGGCATTAGTCCTTCCATTGCGCCCGCCCAATTGCGCGGTGTGATCCTGATGTGCGGCGTCTATG
ATCCGGACAGTTTCGATTTGAAACAGGCACCCGAGGGTCATTTTGCGAAAATCCTCAATGCCTATTTCGGAACGGCCAAT
GCCGCGACCGATCCGCGACTGGCGCAATTTTCAGTGGTGCGCCACGTCACACCAAACTTTCCCCCGATCTTCCTTTCCGT
CGGCAATGGCGATCGCCTGGCTCCGCAATCTCAAAAACTAGCCCAAATCGCCAAAGGACAAGGCGTGTCGGTCGACAGCC
TGTTTTTTGACACCGATCACAAGCCAGCTTTGCCGCATGAATATCAGTTCAATCTCGACCAGGAGGACGGCAAAACCGCT
TTCGAACGTCTGGTTACTTTTCTGAACGCGTCAAGTCATGCTCAATTGAATCAATAA

Upstream 100 bases:

>100_bases
CCACGAAGAATGATCGGATCGTGATCATTGTCGGTGTCCCGTTCGGAACGCCAGGCGCCACCAATCTCATCCGCCTCGCC
CGCGTTTAAAGGAACGGCCC

Downstream 100 bases:

>100_bases
TAGAAATTTGTCTCATCCCCTCTCTCCACAGTGCCGAATTGTTGATTTCATAGGCAAAAGCCATAGACCAGAGCCATTGA
AGGCCATAACGTTTCCGCAT

Product: alpha/beta hydrolase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MKKIMLVSVTALSLVASSHKAPAEPQDTKKEVFAIREGMNNGNAGMEQGLAKHIPDGVASVLDEAYTQGDADARLDVFYP
ASLAQSGQALQTIVWLHGGGWVSGDKDYVAPYLQILAARGFTVVGLNYSLAPEHHYPAPLQQIDAALAYLQKNAARLHID
PNRFFLAGDSAGAQLAGQFGNIVTDPVYARDLGISPSIAPAQLRGVILMCGVYDPDSFDLKQAPEGHFAKILNAYFGTAN
AATDPRLAQFSVVRHVTPNFPPIFLSVGNGDRLAPQSQKLAQIAKGQGVSVDSLFFDTDHKPALPHEYQFNLDQEDGKTA
FERLVTFLNASSHAQLNQ

Sequences:

>Translated_338_residues
MKKIMLVSVTALSLVASSHKAPAEPQDTKKEVFAIREGMNNGNAGMEQGLAKHIPDGVASVLDEAYTQGDADARLDVFYP
ASLAQSGQALQTIVWLHGGGWVSGDKDYVAPYLQILAARGFTVVGLNYSLAPEHHYPAPLQQIDAALAYLQKNAARLHID
PNRFFLAGDSAGAQLAGQFGNIVTDPVYARDLGISPSIAPAQLRGVILMCGVYDPDSFDLKQAPEGHFAKILNAYFGTAN
AATDPRLAQFSVVRHVTPNFPPIFLSVGNGDRLAPQSQKLAQIAKGQGVSVDSLFFDTDHKPALPHEYQFNLDQEDGKTA
FERLVTFLNASSHAQLNQ
>Mature_338_residues
MKKIMLVSVTALSLVASSHKAPAEPQDTKKEVFAIREGMNNGNAGMEQGLAKHIPDGVASVLDEAYTQGDADARLDVFYP
ASLAQSGQALQTIVWLHGGGWVSGDKDYVAPYLQILAARGFTVVGLNYSLAPEHHYPAPLQQIDAALAYLQKNAARLHID
PNRFFLAGDSAGAQLAGQFGNIVTDPVYARDLGISPSIAPAQLRGVILMCGVYDPDSFDLKQAPEGHFAKILNAYFGTAN
AATDPRLAQFSVVRHVTPNFPPIFLSVGNGDRLAPQSQKLAQIAKGQGVSVDSLFFDTDHKPALPHEYQFNLDQEDGKTA
FERLVTFLNASSHAQLNQ

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1786682, Length=123, Percent_Identity=34.1463414634146, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.-

Molecular weight: Translated: 36395; Mature: 36395

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIMLVSVTALSLVASSHKAPAEPQDTKKEVFAIREGMNNGNAGMEQGLAKHIPDGVAS
CCCEEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHHH
VLDEAYTQGDADARLDVFYPASLAQSGQALQTIVWLHGGGWVSGDKDYVAPYLQILAARG
HHHHHHHCCCCCCEEEEEECHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCC
FTVVGLNYSLAPEHHYPAPLQQIDAALAYLQKNAARLHIDPNRFFLAGDSAGAQLAGQFG
CEEEEECEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCEEEEECCCCCHHHHHHHC
NIVTDPVYARDLGISPSIAPAQLRGVILMCGVYDPDSFDLKQAPEGHFAKILNAYFGTAN
CCCCCCHHHHHCCCCCCCCHHHHCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCC
AATDPRLAQFSVVRHVTPNFPPIFLSVGNGDRLAPQSQKLAQIAKGQGVSVDSLFFDTDH
CCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCCCCEEEEECCC
KPALPHEYQFNLDQEDGKTAFERLVTFLNASSHAQLNQ
CCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKKIMLVSVTALSLVASSHKAPAEPQDTKKEVFAIREGMNNGNAGMEQGLAKHIPDGVAS
CCCEEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHHH
VLDEAYTQGDADARLDVFYPASLAQSGQALQTIVWLHGGGWVSGDKDYVAPYLQILAARG
HHHHHHHCCCCCCEEEEEECHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCC
FTVVGLNYSLAPEHHYPAPLQQIDAALAYLQKNAARLHIDPNRFFLAGDSAGAQLAGQFG
CEEEEECEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCEEEEECCCCCHHHHHHHC
NIVTDPVYARDLGISPSIAPAQLRGVILMCGVYDPDSFDLKQAPEGHFAKILNAYFGTAN
CCCCCCHHHHHCCCCCCCCHHHHCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCC
AATDPRLAQFSVVRHVTPNFPPIFLSVGNGDRLAPQSQKLAQIAKGQGVSVDSLFFDTDH
CCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCCCCEEEEECCC
KPALPHEYQFNLDQEDGKTAFERLVTFLNASSHAQLNQ
CCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA