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The map label for this gene is yafC [C]

Identifier: 182678802

GI number: 182678802

Start: 2083888

End: 2084772

Strand: Reverse

Name: yafC [C]

Synonym: Bind_1833

Alternate gene names: 182678802

Gene position: 2084772-2083888 (Counterclockwise)

Preceding gene: 182678808

Following gene: 182678799

Centisome position: 49.99

GC content: 62.71

Gene sequence:

>885_bases
TTGGACAGGCTGCTAGCGATCGAAACTTTCGTGCGGGTGGTGGACACCGGCTCCTTTTCGGCGGCTGCGCGCATCCAAAA
CATCGGCCAGCCCACTGTTTCCAAAGCGATTGCGCAGCTGGAGGATTGGCTCGGCGTCCGCTTGCTTTTGCGCTCGACTC
GCGGCTTGGCGCCGACCGAAGCGGGAATGAGTTTTTACGAGCGGGCGCGACGCGCGATCGAGGAGGCCGATGAAGCGGTG
CTCGCCGCCCGCGGGACGGCCGCTGGCCTTTCTGGGCGGGTGCGAGTCTCGGCGGCGGTCTGTTTTGCCCGCCTGCACAT
CGTGCCCAAGCTGCCGGCCTTCCTTGCGACCCATCCCAACGTCGAAGTTGATCTCCTCCTTGAGGATCGCAACATCGACC
TTGTGGCAGAGGGAGTCGACGTCGCTTTGCGTATGGGGCCGCTCACAGACCCCGGCATGACCGTCCGCAAGATCGCGGAA
CGACGACGGCTCGTGATGGCGACGCCCGAATATTTTGAACGCTACGGGAAGCCAGAAACGCCGAGCGACCTAATCGGACA
CCAGGCAGTGATCTACACACGGGATAACGGTGGCGAGGATTTCACCTTCACGCGCAACACCGAGCAAACCTCAATCGGCC
TTTCCGGCCGTGTGCGCATCAGCGCAACCGAAGGGTTGCGCGCGGCCGTGCTCGCCGGCATCGGCCTGACGATTGCCTCC
GAGTTCGCTTTTTCACCTGAATTGAAGACGGGGGAGGTGGTAACCGCGCTAGACGACTGGACGTTGCCGGCGATCAGCCT
GTCCGCAGTCTATCCCACAGGCCGCATGGCGAGCACGAAGGCCCGGGCCTTCGTGTCCTTCGTCGAGGAATGTCTCAAGA
ATTGA

Upstream 100 bases:

>100_bases
GCTGGCAGCTCATGCTGCGCTGTGGCCTAACCTGCTCATTCTCGATCATTGGCAGAAGGCCGGCTGTTGGATATGATTTA
TTCCAAAAAGGAATGAATAA

Downstream 100 bases:

>100_bases
CCAATGAGGCATCAGGTTTCTGTCATTTAGGCAGCAAGCCTGGGAATCATCTGTTCGACCAATCGCAAGAATCGGGTGTT
GCGTGCCCCCGCAACCCACT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MDRLLAIETFVRVVDTGSFSAAARIQNIGQPTVSKAIAQLEDWLGVRLLLRSTRGLAPTEAGMSFYERARRAIEEADEAV
LAARGTAAGLSGRVRVSAAVCFARLHIVPKLPAFLATHPNVEVDLLLEDRNIDLVAEGVDVALRMGPLTDPGMTVRKIAE
RRRLVMATPEYFERYGKPETPSDLIGHQAVIYTRDNGGEDFTFTRNTEQTSIGLSGRVRISATEGLRAAVLAGIGLTIAS
EFAFSPELKTGEVVTALDDWTLPAISLSAVYPTGRMASTKARAFVSFVEECLKN

Sequences:

>Translated_294_residues
MDRLLAIETFVRVVDTGSFSAAARIQNIGQPTVSKAIAQLEDWLGVRLLLRSTRGLAPTEAGMSFYERARRAIEEADEAV
LAARGTAAGLSGRVRVSAAVCFARLHIVPKLPAFLATHPNVEVDLLLEDRNIDLVAEGVDVALRMGPLTDPGMTVRKIAE
RRRLVMATPEYFERYGKPETPSDLIGHQAVIYTRDNGGEDFTFTRNTEQTSIGLSGRVRISATEGLRAAVLAGIGLTIAS
EFAFSPELKTGEVVTALDDWTLPAISLSAVYPTGRMASTKARAFVSFVEECLKN
>Mature_294_residues
MDRLLAIETFVRVVDTGSFSAAARIQNIGQPTVSKAIAQLEDWLGVRLLLRSTRGLAPTEAGMSFYERARRAIEEADEAV
LAARGTAAGLSGRVRVSAAVCFARLHIVPKLPAFLATHPNVEVDLLLEDRNIDLVAEGVDVALRMGPLTDPGMTVRKIAE
RRRLVMATPEYFERYGKPETPSDLIGHQAVIYTRDNGGEDFTFTRNTEQTSIGLSGRVRISATEGLRAAVLAGIGLTIAS
EFAFSPELKTGEVVTALDDWTLPAISLSAVYPTGRMASTKARAFVSFVEECLKN

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=291, Percent_Identity=34.3642611683849, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI145693193, Length=296, Percent_Identity=30.4054054054054, Blast_Score=151, Evalue=5e-38,
Organism=Escherichia coli, GI1787128, Length=282, Percent_Identity=30.8510638297872, Blast_Score=137, Evalue=6e-34,
Organism=Escherichia coli, GI1789440, Length=283, Percent_Identity=28.9752650176678, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI87081978, Length=285, Percent_Identity=29.4736842105263, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1789639, Length=196, Percent_Identity=33.6734693877551, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1787589, Length=287, Percent_Identity=26.8292682926829, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1789173, Length=254, Percent_Identity=28.740157480315, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788297, Length=120, Percent_Identity=36.6666666666667, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788706, Length=295, Percent_Identity=21.6949152542373, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787601, Length=178, Percent_Identity=28.0898876404494, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI157672245, Length=150, Percent_Identity=30, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788887, Length=292, Percent_Identity=27.0547945205479, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1789204, Length=281, Percent_Identity=23.8434163701068, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1786448, Length=276, Percent_Identity=24.2753623188406, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31864; Mature: 31864

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRLLAIETFVRVVDTGSFSAAARIQNIGQPTVSKAIAQLEDWLGVRLLLRSTRGLAPTE
CCCHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
AGMSFYERARRAIEEADEAVLAARGTAAGLSGRVRVSAAVCFARLHIVPKLPAFLATHPN
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHCCCHHHHCCCC
VEVDLLLEDRNIDLVAEGVDVALRMGPLTDPGMTVRKIAERRRLVMATPEYFERYGKPET
CEEEEEEECCCCEEEECCCEEEEEECCCCCCCHHHHHHHHHCCEEEECHHHHHHCCCCCC
PSDLIGHQAVIYTRDNGGEDFTFTRNTEQTSIGLSGRVRISATEGLRAAVLAGIGLTIAS
CHHHHCCEEEEEEECCCCCCEEEECCCCCCEECCCCEEEEEECCCHHHHHHHHHHHHHHH
EFAFSPELKTGEVVTALDDWTLPAISLSAVYPTGRMASTKARAFVSFVEECLKN
HHCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDRLLAIETFVRVVDTGSFSAAARIQNIGQPTVSKAIAQLEDWLGVRLLLRSTRGLAPTE
CCCHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
AGMSFYERARRAIEEADEAVLAARGTAAGLSGRVRVSAAVCFARLHIVPKLPAFLATHPN
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHCCCHHHHCCCC
VEVDLLLEDRNIDLVAEGVDVALRMGPLTDPGMTVRKIAERRRLVMATPEYFERYGKPET
CEEEEEEECCCCEEEECCCEEEEEECCCCCCCHHHHHHHHHCCEEEECHHHHHHCCCCCC
PSDLIGHQAVIYTRDNGGEDFTFTRNTEQTSIGLSGRVRISATEGLRAAVLAGIGLTIAS
CHHHHCCEEEEEEECCCCCCEEEECCCCCCEECCCCEEEEEECCCHHHHHHHHHHHHHHH
EFAFSPELKTGEVVTALDDWTLPAISLSAVYPTGRMASTKARAFVSFVEECLKN
HHCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]