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The map label for this gene is dhfrIII [H]

Identifier: 182678702

GI number: 182678702

Start: 1956084

End: 1956680

Strand: Direct

Name: dhfrIII [H]

Synonym: Bind_1729

Alternate gene names: 182678702

Gene position: 1956084-1956680 (Clockwise)

Preceding gene: 182678701

Following gene: 182678703

Centisome position: 46.91

GC content: 58.63

Gene sequence:

>597_bases
GTGAGAGCTCTGTTTCTCCGTTCCTTGCCCAATCATAAGCTGGATCCCGCCTTGGGGGATAACGACTTTCCTCTTACAAT
CGTCGCGGCCCTTGCCGACAATCGCGTCATCGGGCTGAATCAGGCCATGCCTTGGGATCTTCCTGGTGATCGCCGTCATT
TTCGCGAGCGGACCTGGGGCAAACCCATGCTGATGGGGCGCAAAACCTTCGAGTCTATCGGCCGCGCCCTGCCCGGGCGC
GAAACCATTGTCCTGACGAGAGACGAGACCTTTGCCGCGCCCGGCGTCTTGGTCGCGCATGATCTTGAAGGCGCGCTCGG
CCTCGCACACGATTGGGCCAGAATCATGGGCGCCAAGACGATCATTCTGGTCGGCGGCGGCGAGCTTTTCGCCGCTCTGT
TGCCAAGGGTCGAACGGCTCGAACTCACCCTTGTCGAGGGGAATCCAAAAGGCGATACATTCTTCCCTCCGATCGATTGG
TCACAATGGGAGGAAGAGAAGCGATCAGGCCCACTGCAGGGTCCAAACGACACAATCTCTTACCAATTCGTCGATTATCA
TCGGAAGCTTGGCGCCGGCGCGACTATCCACTCATGA

Upstream 100 bases:

>100_bases
CTCGCGGCCCTCTAAGCCGTTTCAAGCAAAGCCTTTTTGCTGAGTGACAAGAAAAGGCTCATTCGATGAGGCAGAGCTTT
CACACGACTCGAGGAAGCGT

Downstream 100 bases:

>100_bases
TTGCTTGACTTACAAAACGCATCAGGAAGGATATCCGGCGATGCATGACCTCTATGCGCAGCGAACCTGGAAAAGATGAA
TGCCCTGGAGCAATAACGGC

Product: dihydrofolate reductase

Products: NA

Alternate protein names: Dihydrofolate reductase type III [H]

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MRALFLRSLPNHKLDPALGDNDFPLTIVAALADNRVIGLNQAMPWDLPGDRRHFRERTWGKPMLMGRKTFESIGRALPGR
ETIVLTRDETFAAPGVLVAHDLEGALGLAHDWARIMGAKTIILVGGGELFAALLPRVERLELTLVEGNPKGDTFFPPIDW
SQWEEEKRSGPLQGPNDTISYQFVDYHRKLGAGATIHS

Sequences:

>Translated_198_residues
MRALFLRSLPNHKLDPALGDNDFPLTIVAALADNRVIGLNQAMPWDLPGDRRHFRERTWGKPMLMGRKTFESIGRALPGR
ETIVLTRDETFAAPGVLVAHDLEGALGLAHDWARIMGAKTIILVGGGELFAALLPRVERLELTLVEGNPKGDTFFPPIDW
SQWEEEKRSGPLQGPNDTISYQFVDYHRKLGAGATIHS
>Mature_198_residues
MRALFLRSLPNHKLDPALGDNDFPLTIVAALADNRVIGLNQAMPWDLPGDRRHFRERTWGKPMLMGRKTFESIGRALPGR
ETIVLTRDETFAAPGVLVAHDLEGALGLAHDWARIMGAKTIILVGGGELFAALLPRVERLELTLVEGNPKGDTFFPPIDW
SQWEEEKRSGPLQGPNDTISYQFVDYHRKLGAGATIHS

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Escherichia coli, GI1786233, Length=169, Percent_Identity=35.5029585798817, Blast_Score=104, Evalue=4e-24,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 21986; Mature: 21986

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRALFLRSLPNHKLDPALGDNDFPLTIVAALADNRVIGLNQAMPWDLPGDRRHFRERTWG
CCCEEECCCCCCCCCCCCCCCCCCCEEEEEECCCEEEECCCCCCCCCCCCHHHHHHHCCC
KPMLMGRKTFESIGRALPGRETIVLTRDETFAAPGVLVAHDLEGALGLAHDWARIMGAKT
CCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCE
IILVGGGELFAALLPRVERLELTLVEGNPKGDTFFPPIDWSQWEEEKRSGPLQGPNDTIS
EEEEECHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCEE
YQFVDYHRKLGAGATIHS
EEEEHHHHHHCCCCCCCC
>Mature Secondary Structure
MRALFLRSLPNHKLDPALGDNDFPLTIVAALADNRVIGLNQAMPWDLPGDRRHFRERTWG
CCCEEECCCCCCCCCCCCCCCCCCCEEEEEECCCEEEECCCCCCCCCCCCHHHHHHHCCC
KPMLMGRKTFESIGRALPGRETIVLTRDETFAAPGVLVAHDLEGALGLAHDWARIMGAKT
CCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCE
IILVGGGELFAALLPRVERLELTLVEGNPKGDTFFPPIDWSQWEEEKRSGPLQGPNDTIS
EEEEECHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCEE
YQFVDYHRKLGAGATIHS
EEEEHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840679; 6371010 [H]