Click here to switch to the map view.

The map label for this gene is lipB [H]

Identifier: 182678595

GI number: 182678595

Start: 1832423

End: 1833142

Strand: Direct

Name: lipB [H]

Synonym: Bind_1621

Alternate gene names: 182678595

Gene position: 1832423-1833142 (Clockwise)

Preceding gene: 182678594

Following gene: 182678596

Centisome position: 43.94

GC content: 61.39

Gene sequence:

>720_bases
ATGCTGACACGCGACAGCTTGACCCCTGCCATGCTGCCTGAGGCCGATGGCACCGGCATCGAATGGCGGATTTCGGAAAC
TTATGTTCCTTATCCGGAAGCCTTGGCGCTGATGGAGGAGCGGGCCGCAGCCATTGCCGAGGGCACTGCCGGCGAGCTTG
TCTGGCTGGTCGAGCACCCTCCTCTCTATACCGCCGGCACTTCGGCGCATGATTCTGATCTTCTCGATCAACGGTTTCCT
GTCTATCGCGCCGGGCGCGGCGGCCAGTTCACTTACCATGGCCCGGGCCAGCGCGTGGTCTATGTCATGCTTGATCTGAA
GCGGCGCGTCCCCGATCTTCGCGCTTATGTCGCTGCCCTCGAAGCCTGGATCATCGACACTTTGGCCTCCTTCAACATCA
AGGGGGAACGGCGTGAGGACCGGGTGGGCGTGTGGGTCGCACGGCCCGACAAACCGTCAGGTTTGGGCGGTGAAATGGCC
GAGGATAAGATTGCCGCTATCGGCATCAGGGTCCGCCGCTGGGTCACGTTCCACGGCATTGCCTTGAATGTCGAGCCGGA
TCTGTCGCATTTTTCAGGCATCGTGCCCTGTGGCATTGCCAGCGCGCATTATGGCGTGACGAGCCTCGTCGATCTCGGCC
TTCCAGTCAGTATGGACGATGCCGATCTGGCCCTGCGCCAGGCTTTCGAGCCGATTTTCGGCAAGACGTACCTGCCCTGA

Upstream 100 bases:

>100_bases
ACTTTCTCTTGCGATCGGATTGGGGCAAAGTCGCCAAGAGCGTTGGTGGCGGCTTTCGAGTAAGGTTCATGGCCCCTCCC
TTTTCTTTCCGGAGATCTTC

Downstream 100 bases:

>100_bases
ATATCTCTCGGGAAATTCCCCTTCACGTGGATTTCACAAGAGCTTTGGCTTCGCTTTTGCTTGTTGAGGAAGAGCTTCCG
AAGGTTTTGCGCCTGGGGAA

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MLTRDSLTPAMLPEADGTGIEWRISETYVPYPEALALMEERAAAIAEGTAGELVWLVEHPPLYTAGTSAHDSDLLDQRFP
VYRAGRGGQFTYHGPGQRVVYVMLDLKRRVPDLRAYVAALEAWIIDTLASFNIKGERREDRVGVWVARPDKPSGLGGEMA
EDKIAAIGIRVRRWVTFHGIALNVEPDLSHFSGIVPCGIASAHYGVTSLVDLGLPVSMDDADLALRQAFEPIFGKTYLP

Sequences:

>Translated_239_residues
MLTRDSLTPAMLPEADGTGIEWRISETYVPYPEALALMEERAAAIAEGTAGELVWLVEHPPLYTAGTSAHDSDLLDQRFP
VYRAGRGGQFTYHGPGQRVVYVMLDLKRRVPDLRAYVAALEAWIIDTLASFNIKGERREDRVGVWVARPDKPSGLGGEMA
EDKIAAIGIRVRRWVTFHGIALNVEPDLSHFSGIVPCGIASAHYGVTSLVDLGLPVSMDDADLALRQAFEPIFGKTYLP
>Mature_239_residues
MLTRDSLTPAMLPEADGTGIEWRISETYVPYPEALALMEERAAAIAEGTAGELVWLVEHPPLYTAGTSAHDSDLLDQRFP
VYRAGRGGQFTYHGPGQRVVYVMLDLKRRVPDLRAYVAALEAWIIDTLASFNIKGERREDRVGVWVARPDKPSGLGGEMA
EDKIAAIGIRVRRWVTFHGIALNVEPDLSHFSGIVPCGIASAHYGVTSLVDLGLPVSMDDADLALRQAFEPIFGKTYLP

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family [H]

Homologues:

Organism=Homo sapiens, GI221554520, Length=225, Percent_Identity=35.1111111111111, Blast_Score=79, Evalue=4e-15,
Organism=Escherichia coli, GI87081767, Length=172, Percent_Identity=41.8604651162791, Blast_Score=126, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6323268, Length=168, Percent_Identity=32.7380952380952, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21355483, Length=187, Percent_Identity=39.0374331550802, Blast_Score=108, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 26231; Mature: 26231

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS01313 LIPB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRDSLTPAMLPEADGTGIEWRISETYVPYPEALALMEERAAAIAEGTAGELVWLVEHP
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECC
PLYTAGTSAHDSDLLDQRFPVYRAGRGGQFTYHGPGQRVVYVMLDLKRRVPDLRAYVAAL
CEEECCCCCCCHHHHHHCCCEEECCCCCCEEEECCCCEEEEEEEEHHHCCHHHHHHHHHH
EAWIIDTLASFNIKGERREDRVGVWVARPDKPSGLGGEMAEDKIAAIGIRVRRWVTFHGI
HHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHEEEEEEEEEEEEE
ALNVEPDLSHFSGIVPCGIASAHYGVTSLVDLGLPVSMDDADLALRQAFEPIFGKTYLP
EEEECCCHHHHCCCCCCCCCHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLTRDSLTPAMLPEADGTGIEWRISETYVPYPEALALMEERAAAIAEGTAGELVWLVEHP
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECC
PLYTAGTSAHDSDLLDQRFPVYRAGRGGQFTYHGPGQRVVYVMLDLKRRVPDLRAYVAAL
CEEECCCCCCCHHHHHHCCCEEECCCCCCEEEECCCCEEEEEEEEHHHCCHHHHHHHHHH
EAWIIDTLASFNIKGERREDRVGVWVARPDKPSGLGGEMAEDKIAAIGIRVRRWVTFHGI
HHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHEEEEEEEEEEEEE
ALNVEPDLSHFSGIVPCGIASAHYGVTSLVDLGLPVSMDDADLALRQAFEPIFGKTYLP
EEEECCCHHHHCCCCCCCCCHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA