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The map label for this gene is dus [H]

Identifier: 182678493

GI number: 182678493

Start: 1716935

End: 1717987

Strand: Direct

Name: dus [H]

Synonym: Bind_1517

Alternate gene names: 182678493

Gene position: 1716935-1717987 (Clockwise)

Preceding gene: 182678488

Following gene: 182678494

Centisome position: 41.17

GC content: 63.53

Gene sequence:

>1053_bases
ATGAATTTTGCTGATCCTTTAGGCATACAGCGAACAGCAGCGGGCGAGGAGGCCGGCCCTGTGCGCGCTGGCGCTTTGCG
CATCGGTCCCTTGGAGGTTGCGGGCCGAGCCTTTCTCGCGCCTATGGCTGGCATTACCGATCTTGGCATGCGTCGCTTGG
CGCAACAGTTCGGCGCGGGGCTCGTCGTGTCCGAAATGGTCTCGAGCGATGAATATGCGAAGGGCGACGCCGCCAACAGG
CTCAAAGCGGGCGGGCAGGGGATCGAGCAATCCGTAGTGCAGATTGCTGGTTGCGATCCCGTCGCCATGGGTGAGGCCGC
GCGTCAAGCTGAAGGCTCCGGCGCGGCCATGATCGATATCAATATGGGCTGCCCCGCGCGGAAAGTGATCGGCGGCTATG
CGGGCTCCCATCTCATGCGTGATCTTGATCGCGCCGTGGCCCTGATCCGCGCCGTTATCGGCGCCGTCCGCGTGCCGGTG
AGCGTAAAAATGCGTCTTGGCTGGGATGATCAACAAAGAAACGCCGCCGACCTTGGCCGTCTCGCCGAGGCCGAAGGCGT
GGCCATGCTGAGCGTGCACGGCCGCACCCGCTGCCAGTTCTATCGTGGCAAGGCGGATTGGGCCGCCGTGCGCGCGGTGA
AAGAGGCGGTGACAATACCGGTCGTCGTCAATGGTGATTGCACCGGTCCCGCCGTCGCCTCGGCCATGCTCGCGCAATCG
GGAGCCGATGCGGTCATGATCGGCCGTGCGGCCGTGGGGAAACCCTGGCTTGTGGGCGATATCGCTTATTATCTTGAGAC
GGGTGAGGTTCGTCCCGAGCCCTCGGCTCAGATTCAGGCCGAGGCCACGCTCGACCATTACGAGACCCTGCTTTCTCTTT
ATGGCACGGAGCATGGCGTGCGGCATGCGCGCAAGCATCTTGCGGGCTATGCGGTTCATCGTCACGCGCCGTCCGATCTC
CGGCGCCGCCTCGTCACCAGTGAACATCCTGCCGAGGTTAAATCCATTCTCCTGTCGCTTGCACGCGACACCTTTTGGGC
GGAGGCCGCATGA

Upstream 100 bases:

>100_bases
GATTTGATAGGGGAGCAATGCAGGGATGCGGCATTTTTGATATGGAATTGAGCATGTTCGTGATTGCCGCGCTGTATGAA
ATGATTATAATCTGGGCATG

Downstream 100 bases:

>100_bases
GCGTCCCATCCCTCGTCTTTCGGGCCAATTCCGGCATACTCGCACCATCAGATTCCATGGCTGCCGCGCCCATCGACATC
TCCCCTGACTCCTCCAAACT

Product: NifR3 family TIM-barrel protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MNFADPLGIQRTAAGEEAGPVRAGALRIGPLEVAGRAFLAPMAGITDLGMRRLAQQFGAGLVVSEMVSSDEYAKGDAANR
LKAGGQGIEQSVVQIAGCDPVAMGEAARQAEGSGAAMIDINMGCPARKVIGGYAGSHLMRDLDRAVALIRAVIGAVRVPV
SVKMRLGWDDQQRNAADLGRLAEAEGVAMLSVHGRTRCQFYRGKADWAAVRAVKEAVTIPVVVNGDCTGPAVASAMLAQS
GADAVMIGRAAVGKPWLVGDIAYYLETGEVRPEPSAQIQAEATLDHYETLLSLYGTEHGVRHARKHLAGYAVHRHAPSDL
RRRLVTSEHPAEVKSILLSLARDTFWAEAA

Sequences:

>Translated_350_residues
MNFADPLGIQRTAAGEEAGPVRAGALRIGPLEVAGRAFLAPMAGITDLGMRRLAQQFGAGLVVSEMVSSDEYAKGDAANR
LKAGGQGIEQSVVQIAGCDPVAMGEAARQAEGSGAAMIDINMGCPARKVIGGYAGSHLMRDLDRAVALIRAVIGAVRVPV
SVKMRLGWDDQQRNAADLGRLAEAEGVAMLSVHGRTRCQFYRGKADWAAVRAVKEAVTIPVVVNGDCTGPAVASAMLAQS
GADAVMIGRAAVGKPWLVGDIAYYLETGEVRPEPSAQIQAEATLDHYETLLSLYGTEHGVRHARKHLAGYAVHRHAPSDL
RRRLVTSEHPAEVKSILLSLARDTFWAEAA
>Mature_350_residues
MNFADPLGIQRTAAGEEAGPVRAGALRIGPLEVAGRAFLAPMAGITDLGMRRLAQQFGAGLVVSEMVSSDEYAKGDAANR
LKAGGQGIEQSVVQIAGCDPVAMGEAARQAEGSGAAMIDINMGCPARKVIGGYAGSHLMRDLDRAVALIRAVIGAVRVPV
SVKMRLGWDDQQRNAADLGRLAEAEGVAMLSVHGRTRCQFYRGKADWAAVRAVKEAVTIPVVVNGDCTGPAVASAMLAQS
GADAVMIGRAAVGKPWLVGDIAYYLETGEVRPEPSAQIQAEATLDHYETLLSLYGTEHGVRHARKHLAGYAVHRHAPSDL
RRRLVTSEHPAEVKSILLSLARDTFWAEAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=305, Percent_Identity=28.1967213114754, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI239788462, Length=305, Percent_Identity=28.1967213114754, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI31742496, Length=220, Percent_Identity=30.4545454545455, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI40807366, Length=268, Percent_Identity=26.4925373134328, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI8923374, Length=241, Percent_Identity=25.7261410788382, Blast_Score=79, Evalue=9e-15,
Organism=Escherichia coli, GI1789660, Length=306, Percent_Identity=44.7712418300654, Blast_Score=245, Evalue=2e-66,
Organism=Escherichia coli, GI1788462, Length=261, Percent_Identity=32.5670498084291, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI145693211, Length=245, Percent_Identity=27.3469387755102, Blast_Score=76, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17507177, Length=245, Percent_Identity=28.1632653061224, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI25144369, Length=223, Percent_Identity=28.2511210762332, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17543114, Length=329, Percent_Identity=26.4437689969605, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323560, Length=225, Percent_Identity=29.3333333333333, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6323433, Length=329, Percent_Identity=24.3161094224924, Blast_Score=80, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6323437, Length=258, Percent_Identity=23.2558139534884, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24585320, Length=300, Percent_Identity=29, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24580595, Length=258, Percent_Identity=27.906976744186, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI19920448, Length=258, Percent_Identity=27.906976744186, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI19921524, Length=229, Percent_Identity=30.5676855895196, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI45549423, Length=246, Percent_Identity=30.0813008130081, Blast_Score=89, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 36935; Mature: 36935

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFADPLGIQRTAAGEEAGPVRAGALRIGPLEVAGRAFLAPMAGITDLGMRRLAQQFGAG
CCCCCCCCCCCCCCCCCCCCCEECEEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
LVVSEMVSSDEYAKGDAANRLKAGGQGIEQSVVQIAGCDPVAMGEAARQAEGSGAAMIDI
HHHHHHHCCCCCCCCCHHHHHHCCCCCHHHHHHHHCCCCCHHHCHHHHHCCCCCEEEEEE
NMGCPARKVIGGYAGSHLMRDLDRAVALIRAVIGAVRVPVSVKMRLGWDDQQRNAADLGR
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHCCHHHHHH
LAEAEGVAMLSVHGRTRCQFYRGKADWAAVRAVKEAVTIPVVVNGDCTGPAVASAMLAQS
HHHHCCEEEEEECCCCEEEEECCCCHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHC
GADAVMIGRAAVGKPWLVGDIAYYLETGEVRPEPSAQIQAEATLDHYETLLSLYGTEHGV
CCCEEEECCHHCCCCCHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCHHHH
RHARKHLAGYAVHRHAPSDLRRRLVTSEHPAEVKSILLSLARDTFWAEAA
HHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNFADPLGIQRTAAGEEAGPVRAGALRIGPLEVAGRAFLAPMAGITDLGMRRLAQQFGAG
CCCCCCCCCCCCCCCCCCCCCEECEEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
LVVSEMVSSDEYAKGDAANRLKAGGQGIEQSVVQIAGCDPVAMGEAARQAEGSGAAMIDI
HHHHHHHCCCCCCCCCHHHHHHCCCCCHHHHHHHHCCCCCHHHCHHHHHCCCCCEEEEEE
NMGCPARKVIGGYAGSHLMRDLDRAVALIRAVIGAVRVPVSVKMRLGWDDQQRNAADLGR
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHCCHHHHHH
LAEAEGVAMLSVHGRTRCQFYRGKADWAAVRAVKEAVTIPVVVNGDCTGPAVASAMLAQS
HHHHCCEEEEEECCCCEEEEECCCCHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHC
GADAVMIGRAAVGKPWLVGDIAYYLETGEVRPEPSAQIQAEATLDHYETLLSLYGTEHGV
CCCEEEECCHHCCCCCHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCHHHH
RHARKHLAGYAVHRHAPSDLRRRLVTSEHPAEVKSILLSLARDTFWAEAA
HHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]