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The map label for this gene is ntrB [H]

Identifier: 182678494

GI number: 182678494

Start: 1717984

End: 1719159

Strand: Direct

Name: ntrB [H]

Synonym: Bind_1518

Alternate gene names: 182678494

Gene position: 1717984-1719159 (Clockwise)

Preceding gene: 182678493

Following gene: 182678495

Centisome position: 41.2

GC content: 59.61

Gene sequence:

>1176_bases
ATGAGCGTCCCATCCCTCGTCTTTCGGGCCAATTCCGGCATACTCGCACCATCAGATTCCATGGCTGCCGCGCCCATCGA
CATCTCCCCTGACTCCTCCAAACTGCTGAATGCCTTGCCGCATCCGGTGCTGGCAGTCTTGCCTGATGGCCGGATCGCCG
ACGCCAATGTGGCGGCTGAAGCCTTTTTCGGCATGAGCCGCACGGCGCTCTGGCAACATCGCCTGGTGCATCTCCTGCCG
ATCGGTTCGCCGCTTCTCTCGCTCATCGAGCAGGTGCGCAGCCGGGGCGCGGCGATCAACGAATATCGCGTTGATCTCGG
CCTTCCCCGGATCGAGGGGCTCGTCGATATTTTCGTCTCGCCTTTGCCCGATCTGGATGAAGGCGTCGTCATCATGCTGC
AGGAGCGTTCCATCGCCGATAAGATGGAGCGGCAATTGACGCATCGCGGCGCGGCTCGCTCGGTTGCTGCCATGGCCTCC
ATGCTGGCCCATGAAATCAAGAATCCTCTGTCGGGCATTCGCGGTGCGGCGCAGCTTCTTGAGACCGATGCCAATGATAA
TGATCGCGCCTTGACGCAATTGATCTGCGAGGAGACCGATCGGATTGTGAAGCTCGTCGACCGGATGAGCGCCTTTTCCG
ATCAGCGGCCGATCGAACGCGAGGCCGTCAATATTCATGTGATTCTCGATCACGTGAAGAAAGTGGCCCAGGCCGGCTTC
GCGCGGCATATTCGCTTCGTCGAAAATTACGATCCATCGCTGCCGCCTGTGCTTGGCAATCGCGATCAACTTGTTCAGAT
CCTTCTCAATCTCGTGAAGAATGCGGCGGAAGCCATCGGCGAGGACCGTCTCGATGGCGAGATCGAGCTGTCCACGGCGT
TCCGGCCGGGTGTCCGTCTACAACTCGTCGGCTCCAAGGCCCCCGTTTCTCTGCCGCTTGAATTCTGCGTAAGGGATAAT
GGCGCCGGTGTGCCGGAGGATGTTCTGCCTTATCTCTTCGATCCTTTCATCACGACCAAAGCCACCGGAACTGGCCTTGG
ACTTGCACTGGTTGCGAAGATCATCGGTGACCATGGAGGGATCATCGAATGTGAATCGCATCCGCATCGCACGACGTTCC
GGGTGCTCATGCCCATGTCCGCCTCCCTGGATGGACGCGGGACCGCACGCGGGTAG

Upstream 100 bases:

>100_bases
CGTCCGATCTCCGGCGCCGCCTCGTCACCAGTGAACATCCTGCCGAGGTTAAATCCATTCTCCTGTCGCTTGCACGCGAC
ACCTTTTGGGCGGAGGCCGC

Downstream 100 bases:

>100_bases
GCAGGCGCGAGACGCCGCCCGGCGTCAGGCAGCGATTGCAACAAACCACCAATCAAGATGGGCGGCCTTTCCGCCTTGCA
TCGAGCGCATTTGATCCGAA

Product: signal transduction histidine kinase, nitrogen specific, NtrB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MSVPSLVFRANSGILAPSDSMAAAPIDISPDSSKLLNALPHPVLAVLPDGRIADANVAAEAFFGMSRTALWQHRLVHLLP
IGSPLLSLIEQVRSRGAAINEYRVDLGLPRIEGLVDIFVSPLPDLDEGVVIMLQERSIADKMERQLTHRGAARSVAAMAS
MLAHEIKNPLSGIRGAAQLLETDANDNDRALTQLICEETDRIVKLVDRMSAFSDQRPIEREAVNIHVILDHVKKVAQAGF
ARHIRFVENYDPSLPPVLGNRDQLVQILLNLVKNAAEAIGEDRLDGEIELSTAFRPGVRLQLVGSKAPVSLPLEFCVRDN
GAGVPEDVLPYLFDPFITTKATGTGLGLALVAKIIGDHGGIIECESHPHRTTFRVLMPMSASLDGRGTARG

Sequences:

>Translated_391_residues
MSVPSLVFRANSGILAPSDSMAAAPIDISPDSSKLLNALPHPVLAVLPDGRIADANVAAEAFFGMSRTALWQHRLVHLLP
IGSPLLSLIEQVRSRGAAINEYRVDLGLPRIEGLVDIFVSPLPDLDEGVVIMLQERSIADKMERQLTHRGAARSVAAMAS
MLAHEIKNPLSGIRGAAQLLETDANDNDRALTQLICEETDRIVKLVDRMSAFSDQRPIEREAVNIHVILDHVKKVAQAGF
ARHIRFVENYDPSLPPVLGNRDQLVQILLNLVKNAAEAIGEDRLDGEIELSTAFRPGVRLQLVGSKAPVSLPLEFCVRDN
GAGVPEDVLPYLFDPFITTKATGTGLGLALVAKIIGDHGGIIECESHPHRTTFRVLMPMSASLDGRGTARG
>Mature_390_residues
SVPSLVFRANSGILAPSDSMAAAPIDISPDSSKLLNALPHPVLAVLPDGRIADANVAAEAFFGMSRTALWQHRLVHLLPI
GSPLLSLIEQVRSRGAAINEYRVDLGLPRIEGLVDIFVSPLPDLDEGVVIMLQERSIADKMERQLTHRGAARSVAAMASM
LAHEIKNPLSGIRGAAQLLETDANDNDRALTQLICEETDRIVKLVDRMSAFSDQRPIEREAVNIHVILDHVKKVAQAGFA
RHIRFVENYDPSLPPVLGNRDQLVQILLNLVKNAAEAIGEDRLDGEIELSTAFRPGVRLQLVGSKAPVSLPLEFCVRDNG
AGVPEDVLPYLFDPFITTKATGTGLGLALVAKIIGDHGGIIECESHPHRTTFRVLMPMSASLDGRGTARG

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=358, Percent_Identity=33.7988826815642, Blast_Score=158, Evalue=6e-40,
Organism=Escherichia coli, GI1788549, Length=256, Percent_Identity=29.296875, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1790436, Length=227, Percent_Identity=33.0396475770925, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI1789149, Length=237, Percent_Identity=26.5822784810127, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 42276; Mature: 42145

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVPSLVFRANSGILAPSDSMAAAPIDISPDSSKLLNALPHPVLAVLPDGRIADANVAAE
CCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEECCCCCCCCCHHHHH
AFFGMSRTALWQHRLVHLLPIGSPLLSLIEQVRSRGAAINEYRVDLGLPRIEGLVDIFVS
HHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHC
PLPDLDEGVVIMLQERSIADKMERQLTHRGAARSVAAMASMLAHEIKNPLSGIRGAAQLL
CCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETDANDNDRALTQLICEETDRIVKLVDRMSAFSDQRPIEREAVNIHVILDHVKKVAQAGF
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEHHHHHHHHHHHHHHHH
ARHIRFVENYDPSLPPVLGNRDQLVQILLNLVKNAAEAIGEDRLDGEIELSTAFRPGVRL
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEE
QLVGSKAPVSLPLEFCVRDNGAGVPEDVLPYLFDPFITTKATGTGLGLALVAKIIGDHGG
EEECCCCCCCCCHHHEEECCCCCCCHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHCCCCC
IIECESHPHRTTFRVLMPMSASLDGRGTARG
EEEECCCCCCCEEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
SVPSLVFRANSGILAPSDSMAAAPIDISPDSSKLLNALPHPVLAVLPDGRIADANVAAE
CCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEECCCCCCCCCHHHHH
AFFGMSRTALWQHRLVHLLPIGSPLLSLIEQVRSRGAAINEYRVDLGLPRIEGLVDIFVS
HHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHC
PLPDLDEGVVIMLQERSIADKMERQLTHRGAARSVAAMASMLAHEIKNPLSGIRGAAQLL
CCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETDANDNDRALTQLICEETDRIVKLVDRMSAFSDQRPIEREAVNIHVILDHVKKVAQAGF
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEHHHHHHHHHHHHHHHH
ARHIRFVENYDPSLPPVLGNRDQLVQILLNLVKNAAEAIGEDRLDGEIELSTAFRPGVRL
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEE
QLVGSKAPVSLPLEFCVRDNGAGVPEDVLPYLFDPFITTKATGTGLGLALVAKIIGDHGG
EEECCCCCCCCCHHHEEECCCCCCCHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHCCCCC
IIECESHPHRTTFRVLMPMSASLDGRGTARG
EEEECCCCCCCEEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3020561 [H]