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The map label for this gene is trmE

Identifier: 182678268

GI number: 182678268

Start: 1491469

End: 1492824

Strand: Direct

Name: trmE

Synonym: Bind_1284

Alternate gene names: 182678268

Gene position: 1491469-1492824 (Clockwise)

Preceding gene: 182678265

Following gene: 182678269

Centisome position: 35.77

GC content: 62.17

Gene sequence:

>1356_bases
ATGACGCATTTCGCACAAGCCGAACCGGCCAGCCTGTCTCATGCTGCGACGCTTGAGACAATTTTTGCTCCTGCTTCGGG
CCAGGGGCGTAGCGCCGTTGCCATCGTGCGCGTGTCGGGTCCCAAGGCGGGTTTCCTTCTTGGGGCCTTGGCGGGCTATT
GCCCGGAGCCTCGGCGGGCGACGCTCGCTATTCTGCATGACCCCGAAAATGGTGAAGCGCTCGACGAGGCTCTGGTTCTA
TGGTTCCCGGGGCCGAAAAGTTTCACGGGCGAGGATTGCGCTGAGTTCCATGTGCATGGCGGCCGGGCGGTCATGGCGGG
CTTGTTGGCAGCATTAGGCCGGTTCGAGCAGGTGCGGCCGGCGGAACCGGGAGAATTTACCCGCCGCGCGCTGCTGAACG
GCAAGCTTGATCTTGCCGAGGTCGAAGGTCTCGCCGATATGATCGAGGCCGAGACCGAATGGCAAAGGCGCCAGGCTTTG
CGGCAGATGCGGGGGGCCTTGAGCCGCCAAGCGGAGATGTGGCGGCAAGCCTTGCTCGAGGCCTTGAGCCTGGCCGAGGC
CGAAATCGACTTTTCCGACGAGGCCGATGTTCCTCCAGAAACAAGCCGACGGGTTGCTGCCTTGATCGAACCCGTGCTCG
CGGATTTGCGGGCGGAGCTTGGGCAAGCCCGCGCTGGCGAGCGTATTCGGGAGGGGCTTTCCATCGTCATCATGGGGCCG
CCCAATGCCGGTAAATCCACGCTCCTCAATGCTTTGGCGCGGCGTGAGGTGGCGATTGTCTCGGAGATTGCCGGCACGAC
GCGTGACTTGATCGAAGTCCATCTCGATCTCAAGGGATGTGCTGTGGTTCTGACCGATACGGCGGGCCTCCGGGACAATG
CGGATAAGATCGAACAGATCGGTATTGCCCGCGCTTATGAACGAGGACGCGAGGCTGATCTCGTGCTCTGGTTGAGTGAA
GCCGAGGCTCCGGTCGCGCCTCCAGAAAATTTGGGGATGGAGGTTTGGCCTGTTTTCACCAAGGCCGATCGCGTCGAGCC
CTTAGAAAACAGGGAAGGCCTCGCCATCAGTGCCACGAGCGGGCTTCATCTTGGCCGGCTGGTCGAGGCCATTGCTGATT
TTGCGGGGAAACTCGCGCCCTCGGGCCATGCCGGCCTTATCACGCGGGCGCGCCATCGCCAGGCTTTCGAGCGGGCGGCG
GCGGCGCTCGATCGTTGTATCCGCGAAGCGAGCCCCGTCGAATTGCTGGCCGAGGATCTGCGGCTTGCGGCGCAAGCTTT
ACTGAGCCTGACCGGACGGATCGAGACGGAAGAGATTTTGGGGGAAATTTTTGCGCGTTTCTGTATAGGAAAGTGA

Upstream 100 bases:

>100_bases
GACCACGGGACGAGGCCCCTCAGAATGTTGCTCGCTCATCCTTTATAGGTTTTGGTTTAAAGACCTGATCTTGTCTGAAC
CTTACTCGACAAATGTGAAG

Downstream 100 bases:

>100_bases
GCGCGGCAAATGGAAAGACTGAAACAAGGACGGCCGGACCGATGAAAGGGACCTATGATGTCGTGGTGGTGGGCGGTGGC
CATGCCGGCTGCGAGGCGGC

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MTHFAQAEPASLSHAATLETIFAPASGQGRSAVAIVRVSGPKAGFLLGALAGYCPEPRRATLAILHDPENGEALDEALVL
WFPGPKSFTGEDCAEFHVHGGRAVMAGLLAALGRFEQVRPAEPGEFTRRALLNGKLDLAEVEGLADMIEAETEWQRRQAL
RQMRGALSRQAEMWRQALLEALSLAEAEIDFSDEADVPPETSRRVAALIEPVLADLRAELGQARAGERIREGLSIVIMGP
PNAGKSTLLNALARREVAIVSEIAGTTRDLIEVHLDLKGCAVVLTDTAGLRDNADKIEQIGIARAYERGREADLVLWLSE
AEAPVAPPENLGMEVWPVFTKADRVEPLENREGLAISATSGLHLGRLVEAIADFAGKLAPSGHAGLITRARHRQAFERAA
AALDRCIREASPVELLAEDLRLAAQALLSLTGRIETEEILGEIFARFCIGK

Sequences:

>Translated_451_residues
MTHFAQAEPASLSHAATLETIFAPASGQGRSAVAIVRVSGPKAGFLLGALAGYCPEPRRATLAILHDPENGEALDEALVL
WFPGPKSFTGEDCAEFHVHGGRAVMAGLLAALGRFEQVRPAEPGEFTRRALLNGKLDLAEVEGLADMIEAETEWQRRQAL
RQMRGALSRQAEMWRQALLEALSLAEAEIDFSDEADVPPETSRRVAALIEPVLADLRAELGQARAGERIREGLSIVIMGP
PNAGKSTLLNALARREVAIVSEIAGTTRDLIEVHLDLKGCAVVLTDTAGLRDNADKIEQIGIARAYERGREADLVLWLSE
AEAPVAPPENLGMEVWPVFTKADRVEPLENREGLAISATSGLHLGRLVEAIADFAGKLAPSGHAGLITRARHRQAFERAA
AALDRCIREASPVELLAEDLRLAAQALLSLTGRIETEEILGEIFARFCIGK
>Mature_450_residues
THFAQAEPASLSHAATLETIFAPASGQGRSAVAIVRVSGPKAGFLLGALAGYCPEPRRATLAILHDPENGEALDEALVLW
FPGPKSFTGEDCAEFHVHGGRAVMAGLLAALGRFEQVRPAEPGEFTRRALLNGKLDLAEVEGLADMIEAETEWQRRQALR
QMRGALSRQAEMWRQALLEALSLAEAEIDFSDEADVPPETSRRVAALIEPVLADLRAELGQARAGERIREGLSIVIMGPP
NAGKSTLLNALARREVAIVSEIAGTTRDLIEVHLDLKGCAVVLTDTAGLRDNADKIEQIGIARAYERGREADLVLWLSEA
EAPVAPPENLGMEVWPVFTKADRVEPLENREGLAISATSGLHLGRLVEAIADFAGKLAPSGHAGLITRARHRQAFERAAA
ALDRCIREASPVELLAEDLRLAAQALLSLTGRIETEEILGEIFARFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=459, Percent_Identity=44.6623093681917, Blast_Score=332, Evalue=6e-91,
Organism=Homo sapiens, GI193082993, Length=442, Percent_Identity=45.4751131221719, Blast_Score=324, Evalue=9e-89,
Organism=Homo sapiens, GI193082988, Length=491, Percent_Identity=42.3625254582485, Blast_Score=318, Evalue=5e-87,
Organism=Escherichia coli, GI2367268, Length=462, Percent_Identity=40.2597402597403, Blast_Score=281, Evalue=9e-77,
Organism=Caenorhabditis elegans, GI17507259, Length=451, Percent_Identity=34.1463414634146, Blast_Score=218, Evalue=5e-57,
Organism=Saccharomyces cerevisiae, GI6323665, Length=489, Percent_Identity=31.4928425357873, Blast_Score=239, Evalue=5e-64,
Organism=Drosophila melanogaster, GI45550813, Length=472, Percent_Identity=40.0423728813559, Blast_Score=305, Evalue=4e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_BEII9 (B2IJQ3)

Other databases:

- EMBL:   CP001016
- RefSeq:   YP_001832414.1
- GeneID:   6200966
- GenomeReviews:   CP001016_GR
- KEGG:   bid:Bind_1284
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- ProtClustDB:   PRK05291
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR003593
- InterPro:   IPR018948
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- PRINTS:   PR00326
- SMART:   SM00382
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 48540; Mature: 48409

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: BINDING 37-37 BINDING 95-95 BINDING 135-135 BINDING 451-451

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHFAQAEPASLSHAATLETIFAPASGQGRSAVAIVRVSGPKAGFLLGALAGYCPEPRRA
CCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCE
TLAILHDPENGEALDEALVLWFPGPKSFTGEDCAEFHVHGGRAVMAGLLAALGRFEQVRP
EEEEEECCCCCCHHHCEEEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCC
AEPGEFTRRALLNGKLDLAEVEGLADMIEAETEWQRRQALRQMRGALSRQAEMWRQALLE
CCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALSLAEAEIDFSDEADVPPETSRRVAALIEPVLADLRAELGQARAGERIREGLSIVIMGP
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCEEEEECC
PNAGKSTLLNALARREVAIVSEIAGTTRDLIEVHLDLKGCAVVLTDTAGLRDNADKIEQI
CCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHH
GIARAYERGREADLVLWLSEAEAPVAPPENLGMEVWPVFTKADRVEPLENREGLAISATS
HHHHHHHCCCCCEEEEEEECCCCCCCCCHHCCCEEEEEHHCCCCCCCCCCCCCEEEEECC
GLHLGRLVEAIADFAGKLAPSGHAGLITRARHRQAFERAAAALDRCIREASPVELLAEDL
CCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
RLAAQALLSLTGRIETEEILGEIFARFCIGK
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
THFAQAEPASLSHAATLETIFAPASGQGRSAVAIVRVSGPKAGFLLGALAGYCPEPRRA
CCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCE
TLAILHDPENGEALDEALVLWFPGPKSFTGEDCAEFHVHGGRAVMAGLLAALGRFEQVRP
EEEEEECCCCCCHHHCEEEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCC
AEPGEFTRRALLNGKLDLAEVEGLADMIEAETEWQRRQALRQMRGALSRQAEMWRQALLE
CCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALSLAEAEIDFSDEADVPPETSRRVAALIEPVLADLRAELGQARAGERIREGLSIVIMGP
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCEEEEECC
PNAGKSTLLNALARREVAIVSEIAGTTRDLIEVHLDLKGCAVVLTDTAGLRDNADKIEQI
CCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHH
GIARAYERGREADLVLWLSEAEAPVAPPENLGMEVWPVFTKADRVEPLENREGLAISATS
HHHHHHHCCCCCEEEEEEECCCCCCCCCHHCCCEEEEEHHCCCCCCCCCCCCCEEEEECC
GLHLGRLVEAIADFAGKLAPSGHAGLITRARHRQAFERAAAALDRCIREASPVELLAEDL
CCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
RLAAQALLSLTGRIETEEILGEIFARFCIGK
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA