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The map label for this gene is mnmG

Identifier: 182678269

GI number: 182678269

Start: 1492866

End: 1494722

Strand: Direct

Name: mnmG

Synonym: Bind_1285

Alternate gene names: 182678269

Gene position: 1492866-1494722 (Clockwise)

Preceding gene: 182678268

Following gene: 182678270

Centisome position: 35.8

GC content: 61.34

Gene sequence:

>1857_bases
ATGAAAGGGACCTATGATGTCGTGGTGGTGGGCGGTGGCCATGCCGGCTGCGAGGCGGCGGCCGCCTCGGCGCGGCTCGG
CGCGCGCACGGCTTTGGTCACCCAGGCCTTTGCCACGATCGGGTCCATGTCCTGCAATCCGGCGATTGGCGGTCTCGGCA
AGGGGCATCTTGTGCGCGAGATCGACGCGCTTGACGGGTTGATGGGCCGTGTAGCGGATGCGGCGGGCATTCAGTTCAGG
GTTCTCAATCGCGCCAAAGGTCCCGCCGTGCGCGGTCCACGCGCCCAGGCCGACCGCAAGCTCTACCGTGCCGCCATGCA
GGCGGAAATCCAGGCCGTTAACGGCCTGGATGTGATTGAAGGCGAGGCCGAGGGGCTTTGCTTCGATGGCGAAAAGATCG
CCGGCGTTCTTGTTGGAGTGGAAACCCTGGCCTGCGCCGCCGTGGTGCTGACGACGGGCACTTTTTTGCGCGGGCTGATC
CATATCGGTGAAAAGCAGGTCCGGGCAGGACGGATTGGCGAGGCGCCCGCCGAGGCTATGGGGTTGGCCTTGGAAGCGCT
CGGCCTCAGCATGGGCCGGCTGAAAACCGGCACGCCGGCGAGGCTTGATGGACGCAGCATCGATTGGAGCCGGCTCGAAC
GCCAATCAGGCGATGTCGAGCCTGAGCCCTTTTCTTTTCTGACAGAAGCGATCACGCGACCGCAGGTCGATTGTTTCATC
ACGACGACGACGCCGGAGACCCATGCGGTCATTCGCGAGAATCTGCATTATTCACCGGTCTATTCCGGCGCGATCAATGG
GCGCGGCCCGCGTTATTGCCCCTCGATCGAGGACAAGGTCGTACGCTTTGCCGATCGTGAAGCGCATCAGATCTTTCTCG
AGCCCGAAGGGCTCGATGTGCCGACGATCTATCCCAACGGCATTTCGACAGCTTTGCCAGAGTCTGTCCAGGAGCGCTTT
TTGCGCACGATTCCGGGGCTCGAACATGTCGCCATTCTCCAGCATGGCTATGCGATCGAATATGATTATGTCGATCCGCG
TGAATTGACGGCCGGCCTCGAGGTCAAGCGCGTGCCGGGCCTGTTCCTCGCTGGCCAGATCAATGGGACAACCGGCTATG
AGGAAGCAGGCGCTCAAGGGCTCATCGCTGGACTGAACGCGGCTGCCCGGGCTGGCGGGCTCGCCCCCATCACCTTCGAC
CGTTCGGAAGCCTATCTGGGGGTGATGATCGATGATCTCATCAATCGCGGCGTGTCCGAGCCTTATCGCATGTTTACCTC
ACGGGCCGAATATCGTCTGACCTTGCGGGCCGACAATGCGGATCAAAGATTGACTGGCAAGGGGATGATTCTGGGTTGTA
TTGGTCAGCAACGCGCCGAGATTTTCAAGGCGAAGAGCGAGGCATTGAATTCCGCGCGTGATCTGCTTGCGGGCTTGAGC
CTCAGCCCCACTGAGGCAACGCGGCACGGCCTCAAGGTCAATCGCGACGGCGTCCGGCGCACGGCTTTTGATCTTCTGGC
TCTTCCTGATGTGACACTTGAAACTTTGCGGTCTATCTGGCCAGAATTAAAGACAATTCCTGAGACTATCGCGGCACAGA
TCGAAATCGACGCGCATTATGCCGTTTATCTCGACCGGCAAGAGGCCGATATCGCGGCTTTCCGCCGGGATGAGGGTTTG
GCTCTGCCGACAGATCTCGATTATGCCTCCATGCAGGGGCTTTCCAATGAGATTCGTGGCCGTCTGGCGGCGATACGGCC
CGCGACCTTGGGCCAGGCTGGTCGTATCGAAGGCATGACACCCACGGCGCTCACGCTTCTGGCGGCGCGTGTGCGGCGGG
CTGGCGCTCACGCATGA

Upstream 100 bases:

>100_bases
GGATCGAGACGGAAGAGATTTTGGGGGAAATTTTTGCGCGTTTCTGTATAGGAAAGTGAGCGCGGCAAATGGAAAGACTG
AAACAAGGACGGCCGGACCG

Downstream 100 bases:

>100_bases
AAGGCGTCAAAAAGCAGGTTCAGGGGCTGCCGGACCGCGGACCGGAAAGGCAGGCGGAAGAGGCGGCCATCGCCTCATCA
ATGGCGCCGGAATTACGCGA

Product: tRNA uridine 5-carboxymethylaminomethyl modification protein GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A

Number of amino acids: Translated: 618; Mature: 618

Protein sequence:

>618_residues
MKGTYDVVVVGGGHAGCEAAAASARLGARTALVTQAFATIGSMSCNPAIGGLGKGHLVREIDALDGLMGRVADAAGIQFR
VLNRAKGPAVRGPRAQADRKLYRAAMQAEIQAVNGLDVIEGEAEGLCFDGEKIAGVLVGVETLACAAVVLTTGTFLRGLI
HIGEKQVRAGRIGEAPAEAMGLALEALGLSMGRLKTGTPARLDGRSIDWSRLERQSGDVEPEPFSFLTEAITRPQVDCFI
TTTTPETHAVIRENLHYSPVYSGAINGRGPRYCPSIEDKVVRFADREAHQIFLEPEGLDVPTIYPNGISTALPESVQERF
LRTIPGLEHVAILQHGYAIEYDYVDPRELTAGLEVKRVPGLFLAGQINGTTGYEEAGAQGLIAGLNAAARAGGLAPITFD
RSEAYLGVMIDDLINRGVSEPYRMFTSRAEYRLTLRADNADQRLTGKGMILGCIGQQRAEIFKAKSEALNSARDLLAGLS
LSPTEATRHGLKVNRDGVRRTAFDLLALPDVTLETLRSIWPELKTIPETIAAQIEIDAHYAVYLDRQEADIAAFRRDEGL
ALPTDLDYASMQGLSNEIRGRLAAIRPATLGQAGRIEGMTPTALTLLAARVRRAGAHA

Sequences:

>Translated_618_residues
MKGTYDVVVVGGGHAGCEAAAASARLGARTALVTQAFATIGSMSCNPAIGGLGKGHLVREIDALDGLMGRVADAAGIQFR
VLNRAKGPAVRGPRAQADRKLYRAAMQAEIQAVNGLDVIEGEAEGLCFDGEKIAGVLVGVETLACAAVVLTTGTFLRGLI
HIGEKQVRAGRIGEAPAEAMGLALEALGLSMGRLKTGTPARLDGRSIDWSRLERQSGDVEPEPFSFLTEAITRPQVDCFI
TTTTPETHAVIRENLHYSPVYSGAINGRGPRYCPSIEDKVVRFADREAHQIFLEPEGLDVPTIYPNGISTALPESVQERF
LRTIPGLEHVAILQHGYAIEYDYVDPRELTAGLEVKRVPGLFLAGQINGTTGYEEAGAQGLIAGLNAAARAGGLAPITFD
RSEAYLGVMIDDLINRGVSEPYRMFTSRAEYRLTLRADNADQRLTGKGMILGCIGQQRAEIFKAKSEALNSARDLLAGLS
LSPTEATRHGLKVNRDGVRRTAFDLLALPDVTLETLRSIWPELKTIPETIAAQIEIDAHYAVYLDRQEADIAAFRRDEGL
ALPTDLDYASMQGLSNEIRGRLAAIRPATLGQAGRIEGMTPTALTLLAARVRRAGAHA
>Mature_618_residues
MKGTYDVVVVGGGHAGCEAAAASARLGARTALVTQAFATIGSMSCNPAIGGLGKGHLVREIDALDGLMGRVADAAGIQFR
VLNRAKGPAVRGPRAQADRKLYRAAMQAEIQAVNGLDVIEGEAEGLCFDGEKIAGVLVGVETLACAAVVLTTGTFLRGLI
HIGEKQVRAGRIGEAPAEAMGLALEALGLSMGRLKTGTPARLDGRSIDWSRLERQSGDVEPEPFSFLTEAITRPQVDCFI
TTTTPETHAVIRENLHYSPVYSGAINGRGPRYCPSIEDKVVRFADREAHQIFLEPEGLDVPTIYPNGISTALPESVQERF
LRTIPGLEHVAILQHGYAIEYDYVDPRELTAGLEVKRVPGLFLAGQINGTTGYEEAGAQGLIAGLNAAARAGGLAPITFD
RSEAYLGVMIDDLINRGVSEPYRMFTSRAEYRLTLRADNADQRLTGKGMILGCIGQQRAEIFKAKSEALNSARDLLAGLS
LSPTEATRHGLKVNRDGVRRTAFDLLALPDVTLETLRSIWPELKTIPETIAAQIEIDAHYAVYLDRQEADIAAFRRDEGL
ALPTDLDYASMQGLSNEIRGRLAAIRPATLGQAGRIEGMTPTALTLLAARVRRAGAHA

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family

Homologues:

Organism=Homo sapiens, GI74024895, Length=630, Percent_Identity=46.031746031746, Blast_Score=508, Evalue=1e-144,
Organism=Homo sapiens, GI19882217, Length=655, Percent_Identity=44.2748091603053, Blast_Score=494, Evalue=1e-139,
Organism=Homo sapiens, GI183227703, Length=670, Percent_Identity=43.2835820895522, Blast_Score=489, Evalue=1e-138,
Organism=Escherichia coli, GI2367273, Length=623, Percent_Identity=50.0802568218299, Blast_Score=571, Evalue=1e-164,
Organism=Caenorhabditis elegans, GI17534255, Length=615, Percent_Identity=45.0406504065041, Blast_Score=505, Evalue=1e-143,
Organism=Saccharomyces cerevisiae, GI6321202, Length=631, Percent_Identity=49.1283676703645, Blast_Score=563, Evalue=1e-161,
Organism=Drosophila melanogaster, GI24658174, Length=630, Percent_Identity=47.9365079365079, Blast_Score=551, Evalue=1e-157,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMG_BEII9 (B2IJQ4)

Other databases:

- EMBL:   CP001016
- RefSeq:   YP_001832415.1
- ProteinModelPortal:   B2IJQ4
- SMR:   B2IJQ4
- GeneID:   6201797
- GenomeReviews:   CP001016_GR
- KEGG:   bid:Bind_1285
- HOGENOM:   HBG284774
- OMA:   GIQFRVL
- ProtClustDB:   PRK05192
- GO:   GO:0005737
- HAMAP:   MF_00129
- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00136

Pfam domain/function: PF01134 GIDA

EC number: NA

Molecular weight: Translated: 66136; Mature: 66136

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGTYDVVVVGGGHAGCEAAAASARLGARTALVTQAFATIGSMSCNPAIGGLGKGHLVRE
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
IDALDGLMGRVADAAGIQFRVLNRAKGPAVRGPRAQADRKLYRAAMQAEIQAVNGLDVIE
HHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEC
GEAEGLCFDGEKIAGVLVGVETLACAAVVLTTGTFLRGLIHIGEKQVRAGRIGEAPAEAM
CCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCHHHH
GLALEALGLSMGRLKTGTPARLDGRSIDWSRLERQSGDVEPEPFSFLTEAITRPQVDCFI
HHHHHHHCCHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEEE
TTTTPETHAVIRENLHYSPVYSGAINGRGPRYCPSIEDKVVRFADREAHQIFLEPEGLDV
EECCCCHHHHHHHCCCCCCEECCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCC
PTIYPNGISTALPESVQERFLRTIPGLEHVAILQHGYAIEYDYVDPRELTAGLEVKRVPG
CEECCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCEEEECCCCHHHHHCCCEEECCCC
LFLAGQINGTTGYEEAGAQGLIAGLNAAARAGGLAPITFDRSEAYLGVMIDDLINRGVSE
EEEEEEECCCCCCHHCCCCHHHHHCCHHHHCCCCCEEEECCCCCEEHHHHHHHHHCCCCH
PYRMFTSRAEYRLTLRADNADQRLTGKGMILGCIGQQRAEIFKAKSEALNSARDLLAGLS
HHHHHHCCCEEEEEEEECCCCCEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCC
LSPTEATRHGLKVNRDGVRRTAFDLLALPDVTLETLRSIWPELKTIPETIAAQIEIDAHY
CCCCHHHHCCCEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCEE
AVYLDRQEADIAAFRRDEGLALPTDLDYASMQGLSNEIRGRLAAIRPATLGQAGRIEGMT
EEEEECCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEECCHHCCCCCCCCCCC
PTALTLLAARVRRAGAHA
HHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKGTYDVVVVGGGHAGCEAAAASARLGARTALVTQAFATIGSMSCNPAIGGLGKGHLVRE
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
IDALDGLMGRVADAAGIQFRVLNRAKGPAVRGPRAQADRKLYRAAMQAEIQAVNGLDVIE
HHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEC
GEAEGLCFDGEKIAGVLVGVETLACAAVVLTTGTFLRGLIHIGEKQVRAGRIGEAPAEAM
CCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCHHHH
GLALEALGLSMGRLKTGTPARLDGRSIDWSRLERQSGDVEPEPFSFLTEAITRPQVDCFI
HHHHHHHCCHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEEE
TTTTPETHAVIRENLHYSPVYSGAINGRGPRYCPSIEDKVVRFADREAHQIFLEPEGLDV
EECCCCHHHHHHHCCCCCCEECCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCC
PTIYPNGISTALPESVQERFLRTIPGLEHVAILQHGYAIEYDYVDPRELTAGLEVKRVPG
CEECCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCEEEECCCCHHHHHCCCEEECCCC
LFLAGQINGTTGYEEAGAQGLIAGLNAAARAGGLAPITFDRSEAYLGVMIDDLINRGVSE
EEEEEEECCCCCCHHCCCCHHHHHCCHHHHCCCCCEEEECCCCCEEHHHHHHHHHCCCCH
PYRMFTSRAEYRLTLRADNADQRLTGKGMILGCIGQQRAEIFKAKSEALNSARDLLAGLS
HHHHHHCCCEEEEEEEECCCCCEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCC
LSPTEATRHGLKVNRDGVRRTAFDLLALPDVTLETLRSIWPELKTIPETIAAQIEIDAHY
CCCCHHHHCCCEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCEE
AVYLDRQEADIAAFRRDEGLALPTDLDYASMQGLSNEIRGRLAAIRPATLGQAGRIEGMT
EEEEECCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEECCHHCCCCCCCCCCC
PTALTLLAARVRRAGAHA
HHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA