Click here to switch to the map view.

The map label for this gene is yddQ [H]

Identifier: 182678232

GI number: 182678232

Start: 1449809

End: 1450381

Strand: Direct

Name: yddQ [H]

Synonym: Bind_1247

Alternate gene names: 182678232

Gene position: 1449809-1450381 (Clockwise)

Preceding gene: 182678230

Following gene: 182678236

Centisome position: 34.77

GC content: 55.67

Gene sequence:

>573_bases
ATGACGAAACGTGCTTTGATCATCGTCGATCTACAGAATGACTATTTTGCTGGTGGTAAATTCACCCTCGTTGGCATCGA
TGCCGCCGCCGCCAACGCCGCGCGTCTTCTGGATGCGGCGCGTGCGGCAGGTGACCTCGTGGTCCATGTCCGCCATGAAT
TCCCATCAGCCGATGCGCCCTTTTTCGTGCCGGGCTCGCATGGTGCCGAGATCAACAAAAGTGTTTTGAACCGTGATAGT
GAGCCTGTCGTGCTCAAGAACTCCATCAATGCCTTCCGTGGCACGGCCCTCAAGGAGATCCTCGACCACAATGGCATTGA
TGAGGTCGTGATTGCCGGCGCCATGAGCCATATCTGTATTGACGCTATCACACGCGCTGCCGTCGATTTCGGTTACAAGG
CAACAGTTGTGCATGATGCTGTTGCCACGCGCGATCTCGAGTTCAATGGCATTATCGTCCCCGCTGCACAGGCGCATGCT
GCTTTCATGGCTGCCTTAGGGTTTGCCTATGCCACGTTGCTTTCAACCGATGAACTGCTCGCCAAGAGCCGCGTACTGGA
TGTCACCGCTTGA

Upstream 100 bases:

>100_bases
TCGTTCTTTGTCGTTCCTGCCGCTCCGTAGCATGCCAGTTCACCTCTATCCTGGCAATCGGGAGGCAATGAGGCCCCTTC
AACAAACCTGGATGGCTGAC

Downstream 100 bases:

>100_bases
TTGCACGAGGATCGGCGCGTGTCTCTCCCGAACATGCAGGGCGATCACGACCAGATTGTACCAATCGCGGATTCTACCCT
GCTTTCGACCATGCGCATGA

Product: isochorismatase hydrolase

Products: Nicotinate; pyrazinoic acid; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 190; Mature: 189

Protein sequence:

>190_residues
MTKRALIIVDLQNDYFAGGKFTLVGIDAAAANAARLLDAARAAGDLVVHVRHEFPSADAPFFVPGSHGAEINKSVLNRDS
EPVVLKNSINAFRGTALKEILDHNGIDEVVIAGAMSHICIDAITRAAVDFGYKATVVHDAVATRDLEFNGIIVPAAQAHA
AFMAALGFAYATLLSTDELLAKSRVLDVTA

Sequences:

>Translated_190_residues
MTKRALIIVDLQNDYFAGGKFTLVGIDAAAANAARLLDAARAAGDLVVHVRHEFPSADAPFFVPGSHGAEINKSVLNRDS
EPVVLKNSINAFRGTALKEILDHNGIDEVVIAGAMSHICIDAITRAAVDFGYKATVVHDAVATRDLEFNGIIVPAAQAHA
AFMAALGFAYATLLSTDELLAKSRVLDVTA
>Mature_189_residues
TKRALIIVDLQNDYFAGGKFTLVGIDAAAANAARLLDAARAAGDLVVHVRHEFPSADAPFFVPGSHGAEINKSVLNRDSE
PVVLKNSINAFRGTALKEILDHNGIDEVVIAGAMSHICIDAITRAAVDFGYKATVVHDAVATRDLEFNGIIVPAAQAHAA
FMAALGFAYATLLSTDELLAKSRVLDVTA

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 20053; Mature: 19922

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKRALIIVDLQNDYFAGGKFTLVGIDAAAANAARLLDAARAAGDLVVHVRHEFPSADAP
CCCCEEEEEECCCCEECCCEEEEEEECHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCC
FFVPGSHGAEINKSVLNRDSEPVVLKNSINAFRGTALKEILDHNGIDEVVIAGAMSHICI
EEECCCCCCHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
DAITRAAVDFGYKATVVHDAVATRDLEFNGIIVPAAQAHAAFMAALGFAYATLLSTDELL
HHHHHHHHHCCCEEEEEEHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHH
AKSRVLDVTA
HHHHEEEECC
>Mature Secondary Structure 
TKRALIIVDLQNDYFAGGKFTLVGIDAAAANAARLLDAARAAGDLVVHVRHEFPSADAP
CCCEEEEEECCCCEECCCEEEEEEECHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCC
FFVPGSHGAEINKSVLNRDSEPVVLKNSINAFRGTALKEILDHNGIDEVVIAGAMSHICI
EEECCCCCCHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
DAITRAAVDFGYKATVVHDAVATRDLEFNGIIVPAAQAHAAFMAALGFAYATLLSTDELL
HHHHHHHHHCCCEEEEEEHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHH
AKSRVLDVTA
HHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Nicotinamide; pyrazinamide; H2O [C]

Specific reaction: Nicotinamide + H2O = Nicotinate + NH3 pyrazinamide + H2O = pyrazinoic acid + NH3 [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]